FastQCFastQC Report
Mon 15 Aug 2016
SRR2921486_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921486_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1878594
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19670.10470596627051934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20650.071.7702941
GTATCAA42000.057.394621
TCAACGC51500.045.7488144
CAACGCA52550.044.9455345
AACGCAG55500.042.4470756
TATCAAC56800.041.793132
ATCAACG57100.041.2609673
GTACATG92100.037.09521
ACGCAGA65250.035.8308947
TACATGG96100.035.1645662
ACATGGG97450.034.5553173
CGCAGAG70100.033.2643478
CATGGGG82700.031.7986474
GCAGAGT77700.029.7810159
CAGAGTA72750.026.24719810-11
AGAGTAC69450.025.78131910-11
GTACTAA3002.237357E-1023.8081381
ATGGGGG41100.023.7394565
ATGGGGC34450.023.4865075
GAGTACT28600.021.31909412-13
Produced by FastQC (version 0.11.5)