##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921486_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1878594
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.743771139479843	31.0	30.0	34.0	25.0	34.0
2	29.77027872973085	31.0	30.0	34.0	25.0	34.0
3	29.839790822285178	31.0	30.0	34.0	25.0	34.0
4	33.19917076281517	37.0	35.0	37.0	26.0	37.0
5	33.19066919195952	37.0	35.0	37.0	26.0	37.0
6	33.20881254810779	37.0	35.0	37.0	26.0	37.0
7	33.01099758649288	37.0	35.0	37.0	25.0	37.0
8	32.97075365938569	37.0	35.0	37.0	25.0	37.0
9	34.41187079273116	39.0	35.0	39.0	25.0	39.0
10-11	34.29533922710282	39.0	35.0	39.0	25.0	39.0
12-13	34.173377802761	39.0	35.0	39.0	23.0	39.0
14-15	35.32303946462088	40.0	36.0	41.0	23.5	41.0
16-17	35.27812582175819	40.0	36.0	41.0	23.0	41.0
18-19	35.30741394894267	40.0	36.0	41.0	23.0	41.0
20-21	35.31774055490436	40.0	35.0	41.0	23.0	41.0
22-23	35.29290043511264	40.0	35.0	41.0	24.0	41.0
24-25	35.27062340239562	40.0	35.0	41.0	24.0	41.0
26-27	35.192760117406955	40.0	35.0	41.0	24.0	41.0
28-29	35.14857521103549	40.0	35.0	41.0	24.0	41.0
30-31	35.046515106510505	40.0	35.0	41.0	24.0	41.0
32-33	34.93447626256658	40.0	35.0	41.0	23.5	41.0
34-35	34.76396203756639	39.5	34.0	41.0	22.5	41.0
36-37	34.63371276603672	39.0	34.0	41.0	21.5	41.0
38-39	34.50907221038713	39.0	34.0	41.0	20.5	41.0
40-41	34.34749631905564	39.0	34.0	41.0	18.5	41.0
42-43	34.14906334205262	39.0	33.0	41.0	17.0	41.0
44-45	33.954659974427685	39.0	33.0	41.0	15.0	41.0
46-47	33.69377018131645	38.5	33.0	41.0	13.5	41.0
48-49	33.53432859894155	38.0	33.0	41.0	10.0	41.0
50-51	33.3318859742978	38.0	33.0	40.0	8.0	41.0
52-53	33.13474997790901	38.0	33.0	40.0	7.5	41.0
54-55	32.928918648734104	38.0	32.0	40.0	7.0	41.0
56-57	32.680931058014664	37.0	32.0	40.0	3.5	41.0
58-59	32.42842120223955	37.0	32.0	40.0	2.0	41.0
60-61	32.11829618320935	36.0	31.5	40.0	2.0	41.0
62-63	31.799644042299718	35.5	31.0	39.0	2.0	41.0
64-65	31.49114976413211	35.0	31.0	39.0	2.0	41.0
66-67	31.435276062842743	35.0	31.0	39.0	2.0	41.0
68-69	31.183931440215396	35.0	31.0	38.5	2.0	40.0
70-71	30.87560217907648	35.0	31.0	37.0	2.0	40.0
72-73	30.553580230747038	35.0	31.0	37.0	2.0	39.0
74-75	30.252092522386423	35.0	30.0	36.0	2.0	39.0
76-77	29.975366683807145	35.0	30.0	36.0	2.0	38.5
78-79	29.713011166862024	35.0	30.0	35.0	2.0	37.0
80-81	29.47935770049303	35.0	30.0	35.0	2.0	37.0
82-83	29.286014168042698	35.0	30.0	35.0	2.0	36.5
84-85	29.095692576469425	35.0	30.0	35.0	2.0	36.0
86-87	28.94660581264499	34.0	29.5	35.0	2.0	36.0
88-89	28.82282015166662	34.0	29.0	35.0	2.0	35.5
90-91	28.720088800453958	34.0	29.0	35.0	2.0	35.0
92-93	28.6257509605588	34.0	29.0	35.0	2.0	35.0
94-95	28.56119789587319	34.0	29.0	35.0	2.0	35.0
96-97	28.46081910194539	34.0	29.0	35.0	2.0	35.0
98-99	28.39966911424182	34.0	29.0	35.0	2.0	35.0
100-101	28.336275693417523	34.0	29.0	35.0	2.0	35.0
102-103	27.39088461902891	32.5	26.5	34.5	2.0	35.0
104-105	28.184162464055568	34.0	29.0	35.0	2.0	35.0
106-107	28.494547784140693	34.5	29.0	35.0	2.0	35.0
108-109	28.532126153921496	35.0	30.0	35.0	2.0	35.0
110-111	28.472749300806882	35.0	30.0	35.0	2.0	35.0
112-113	28.40401145750492	35.0	29.0	35.0	2.0	35.0
114-115	28.28272793376323	35.0	29.0	35.0	2.0	35.0
116-117	28.22252200315768	34.5	29.0	35.0	2.0	35.0
118-119	28.065808524886165	34.0	29.0	35.0	2.0	35.0
120-121	27.89766974662966	34.0	29.0	35.0	2.0	35.0
122-123	27.767214736127123	34.0	29.0	35.0	2.0	35.0
124-125	26.93826420184457	33.5	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	79880.0
3	13617.0
4	8820.0
5	7528.0
6	8023.0
7	9169.0
8	9857.0
9	10443.0
10	11218.0
11	11838.0
12	11923.0
13	12748.0
14	14487.0
15	12348.0
16	8680.0
17	7392.0
18	6697.0
19	6121.0
20	6152.0
21	6413.0
22	7483.0
23	10479.0
24	15181.0
25	20661.0
26	23932.0
27	25466.0
28	30217.0
29	35131.0
30	39838.0
31	47824.0
32	60379.0
33	77485.0
34	108799.0
35	171397.0
36	330723.0
37	478508.0
38	131520.0
39	217.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.96964775420006	16.88735056843899	12.195106365984184	24.947895311376765
2	16.88293640546223	19.108071686084	38.1179075177317	25.891084390722064
3	19.439754433325078	23.636271667452032	28.725603733550575	28.198370165672316
4	13.620551486542972	14.92208528685873	35.2614329491511	36.1959302774472
5	14.603129637626791	37.01475148044629	33.189260168233865	15.192858713693056
6	34.088702638307296	34.144354141670085	16.94021111357859	14.826732106444018
7	30.02337238671528	31.30014692279551	20.169373919313344	18.507106771175867
8	28.17857430025757	33.32112137708267	19.254492020782337	19.24581230187743
9	27.84077476353057	14.61467356462362	18.654408522910007	38.890143148935806
10-11	26.6733655069366	24.73602682092104	26.541279073792147	22.04932859835021
12-13	26.83463546381476	22.431638149725124	26.73750817381525	23.996218212644866
14-15	24.224892370198592	23.538724097626954	24.187165362506757	28.0492181696677
16-17	24.43300330525086	26.503111685741835	24.631680459401647	24.432204549605654
18-19	24.474866025363646	25.616722697466965	26.274073827513895	23.634337449655494
20-21	25.588933812622404	24.560386854242044	25.98152972325477	23.869149609880782
22-23	25.362770968096715	24.22341541433423	25.828845548018435	24.58496806955062
24-25	24.69919290297924	24.70808814815111	25.924712668933093	24.66800627993656
26-27	24.5657004618664	24.636611399805346	26.051182093057623	24.746506045270632
28-29	24.55753726432943	24.7308343369989	26.046619255839786	24.665009142831885
30-31	24.877210444987483	24.679548885354432	25.802995093210885	24.6402455764472
32-33	24.504930785780655	24.839064723237662	25.80562176954372	24.850382721437967
34-35	24.825540047708397	24.783364525646526	25.86443179001276	24.526663636632318
36-37	24.94443831974449	24.6276315572236	25.779341440305746	24.648588682726164
38-39	24.992323472490867	24.817008362022108	25.660454338576276	24.53021382691075
40-41	24.911787848811123	24.767863110398665	25.785907624549488	24.53444141624073
42-43	24.793470303425085	24.781699422294743	25.694901424196726	24.72992885008345
44-45	24.732300715862067	24.783351926058035	25.66578953745793	24.818557820621972
46-47	24.86622527844211	24.937626836972214	25.44651940705546	24.749628477530212
48-49	25.05439493210453	24.979409677518138	25.21847843308511	24.747716957292223
50-51	24.855078657890832	25.26814675634228	25.128264940898404	24.748509644868484
52-53	25.031058762246072	25.075666202127895	25.203443214995342	24.689831820630687
54-55	24.87554849860697	25.02919068379826	25.318661081582405	24.77659973601237
56-57	24.86440025370533	25.15019153048112	25.19023905496363	24.795169160849923
58-59	24.93362449531051	25.020642908438685	25.17047540864438	24.875257187606426
60-61	24.874258604343062	25.095189651119043	25.417374104847195	24.613177639690697
62-63	24.795186073931117	25.231600562785637	25.267120814654582	24.706092548628664
64-65	24.861261625879013	24.948756932924702	25.34365738903992	24.846324052156362
66-67	24.792578459683728	25.173661125308115	25.240649456664993	24.793110958343163
68-69	24.913192826673725	25.09905415990198	25.190400705426423	24.79735230799787
70-71	24.769624950537867	24.926146309925073	25.396349468152245	24.907879271384818
72-73	24.973302907566957	25.21944064832335	25.108419082184447	24.698837361925246
74-75	24.721577648996263	25.375088365331234	25.1542515872477	24.7490823984248
76-77	24.855297727255785	25.062380390991663	25.160756637135158	24.921565244617394
78-79	24.813304433648625	25.130425313653078	25.164424420677317	24.89184583202098
80-81	24.776540350789386	25.318434159835647	24.9895707737807	24.91545471559427
82-83	24.910408958944384	25.254158509724707	25.10055865624353	24.73487387508738
84-85	24.811852738414146	25.220664240826363	24.969339802832373	24.99814321792712
86-87	24.615812182416803	25.291628155600325	25.108425511434056	24.984134150548808
88-89	24.705957206760516	25.148275941933917	25.117022123390818	25.02874472791474
90-91	24.709858926980466	25.13342137142982	25.120511128496315	25.036208573093404
92-93	24.778401091030386	25.314519111000845	25.01958631675819	24.88749348121058
94-95	24.68817846605314	25.13144739069742	25.17520994847518	25.00516419477426
96-97	24.699874040262397	25.09502879612091	25.191521621313704	25.013575542302984
98-99	24.683957733971894	25.173180407881652	25.033486159409023	25.10937569873743
100-101	24.638877073132818	25.245917086931964	25.14090621850672	24.9742996214285
102-103	24.672181065927912	25.101145822464595	25.25305087741339	24.9736222341941
104-105	24.475696837239038	25.276907610158162	25.122819401645863	25.124576150956933
106-107	24.562596657288193	25.29789742948177	25.10781696270526	25.031688950524778
108-109	24.571081302094203	25.217001503464783	25.140018143795974	25.07189905064504
110-111	24.55773730146689	25.361611741501704	25.098844002949217	24.981806954082188
112-113	24.577201459086176	25.345367103271023	25.159840420434872	24.917591017207933
114-115	24.363332532133825	25.51527642580906	25.187583164376058	24.93380787768106
116-117	24.395658333018357	25.52116134108512	25.12341298592615	24.95976733997037
118-119	24.553135018377255	25.28414305655754	25.07763908869319	25.08508283637202
120-121	24.3561543066083	25.352309809863954	25.27172308939089	25.019812794136854
122-123	24.32656212560901	25.48731396927664	25.092036953222756	25.09408695189159
124-125	24.344791093854052	25.537297121195746	25.140309700759587	24.97760208419061
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	140.0
1	175.0
2	217.5
3	213.0
4	193.0
5	184.0
6	202.5
7	249.0
8	328.5
9	413.5
10	452.0
11	475.5
12	547.5
13	660.5
14	747.0
15	829.0
16	928.5
17	1040.5
18	1119.0
19	1210.5
20	1449.0
21	1678.0
22	1904.0
23	2292.0
24	2866.5
25	3587.0
26	4577.0
27	5877.5
28	6982.5
29	8193.5
30	9994.5
31	11912.0
32	14099.5
33	16538.0
34	18989.0
35	21627.5
36	24819.5
37	28207.5
38	30798.0
39	33841.0
40	37670.5
41	40902.0
42	44069.5
43	49090.0
44	54096.0
45	57594.0
46	61873.5
47	66477.5
48	71904.5
49	76762.0
50	77790.5
51	78146.5
52	78410.5
53	79588.5
54	81242.5
55	81056.0
56	78305.0
57	72575.0
58	68191.0
59	63749.0
60	56719.5
61	49278.0
62	41997.5
63	33277.5
64	25209.5
65	19720.0
66	15157.0
67	11719.0
68	9492.5
69	7657.0
70	6332.5
71	5140.5
72	4038.5
73	3480.5
74	2879.0
75	2308.0
76	1671.0
77	972.0
78	575.5
79	357.0
80	197.0
81	94.0
82	68.0
83	44.5
84	21.5
85	10.0
86	12.5
87	17.0
88	15.0
89	12.0
90	12.0
91	13.0
92	11.0
93	7.0
94	8.0
95	11.0
96	10.0
97	6.0
98	7.5
99	9.0
100	23.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03428095692842626
2	0.02453962910559706
3	0.026030105493789507
4	0.030820922455836654
5	0.03263078664149891
6	0.04455459774703848
7	0.039231467789208316
8	0.03476003862463097
9	0.029277214768065905
10-11	0.03497296382294418
12-13	0.03438741952758286
14-15	0.03348248743475173
16-17	0.03582466461619701
18-19	0.048254173067730444
20-21	0.0580753478399271
22-23	0.053098221329355884
24-25	0.06315893694965491
26-27	0.047189547076164406
28-29	0.04756216617321252
30-31	0.047349240974899315
32-33	0.05607917410574079
34-35	0.038725770443214444
36-37	0.05062296589896486
38-39	0.05948597727875209
40-41	0.046657234080381395
42-43	0.05746318789477663
44-45	0.05682441229983701
46-47	0.06302585870070915
48-49	0.047455703574055916
50-51	0.049904343354657786
52-53	0.05879397038423417
54-55	0.04548614548965876
56-57	0.043915822152098855
58-59	0.04420859429977952
60-61	0.03366879698327579
62-63	0.0421591892660149
64-65	0.04146718237149698
66-67	0.03486650122378757
68-69	0.030927385054993255
70-71	0.04764201312257997
72-73	0.0309007694052041
74-75	0.03920485213941916
76-77	0.03193877974698099
78-79	0.03212508929550504
80-81	0.024406550856651305
82-83	0.017938947957887655
84-85	0.018338182704724915
86-87	0.01841802965409237
88-89	0.022543455371410746
90-91	0.012802127548581545
92-93	0.01966896519418246
94-95	0.014691838683611255
96-97	0.011497960708913155
98-99	0.010965647713130139
100-101	0.011551192008491458
102-103	0.0062812933502395946
104-105	0.006627296797498555
106-107	0.009528402624515995
108-109	0.014159525687828238
110-111	0.006866837645600912
112-113	0.00841054533337166
114-115	0.008543623582317413
116-117	0.0077185384388537386
118-119	0.2415636374863329
120-121	0.35651662892567526
122-123	0.028957826970596092
124-125	0.003779422270059417
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1878594.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.43133493263456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.45340649182235	47.814533476868895
2	10.413744177155436	12.378054361905951
3	3.392159847314232	6.048017640923
4	1.685782376430507	4.0075318814869645
5	0.9553584552357729	2.8389114166920795
6	0.6389390594681371	2.278380074687602
7	0.4307198264111023	1.7918777985895105
8	0.305761285210822	1.4537441080637732
9	0.2343740283311082	1.2536245239531185
>10	1.3987894553656053	14.848119079014921
>50	0.0682023072509164	2.7383202988836874
>100	0.02213061993224527	2.166261343487046
>500	3.611828981528188E-4	0.1355014850623775
>1k	2.7088717361461366E-4	0.24712251038108818
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1967	0.10470596627051934	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.3231299578301645E-5	5.3231299578301645E-5
10-11	0.0	0.0	0.0	5.3231299578301645E-5	5.3231299578301645E-5
12-13	0.0	0.0	0.0	5.3231299578301645E-5	1.8630954852405577E-4
14-15	0.0	0.0	0.0	5.3231299578301645E-5	2.3954084810235739E-4
16-17	0.0	0.0	0.0	1.0646259915660329E-4	2.661564978915082E-4
18-19	0.0	0.0	0.0	1.0646259915660329E-4	3.193877974698099E-4
20-21	0.0	2.6615649789150823E-5	0.0	1.0646259915660329E-4	3.7261909704811155E-4
22-23	0.0	5.3231299578301645E-5	0.0	1.5969389873490494E-4	4.52466046415564E-4
24-25	0.0	5.3231299578301645E-5	0.0	2.661564978915082E-4	5.323129957830164E-4
26-27	0.0	5.3231299578301645E-5	0.0	4.2585039662641316E-4	6.387755949396198E-4
28-29	0.0	5.3231299578301645E-5	0.0	0.0015703233375598984	6.387755949396198E-4
30-31	0.0	5.3231299578301645E-5	0.0	0.004045578767950924	6.387755949396198E-4
32-33	0.0	5.3231299578301645E-5	0.0	0.009209014827046185	6.387755949396198E-4
34-35	0.0	5.3231299578301645E-5	0.0	0.017034015865056526	6.920068945179214E-4
36-37	0.0	5.3231299578301645E-5	0.0	0.028292435725867326	6.920068945179214E-4
38-39	0.0	5.3231299578301645E-5	0.0	0.0469233905782729	8.250851434636755E-4
40-41	0.0	5.3231299578301645E-5	0.0	0.06512849503405206	9.04932092831128E-4
42-43	0.0	5.3231299578301645E-5	0.0	0.08479746022823452	9.581633924094297E-4
44-45	0.0	5.3231299578301645E-5	0.0	0.1069949121523863	0.0010380103417768822
46-47	0.0	5.3231299578301645E-5	0.0	0.1334508680428022	0.0010646259915660329
48-49	0.0	5.3231299578301645E-5	0.0	0.1633934740555969	0.0011710885907226362
50-51	0.0	5.3231299578301645E-5	0.0	0.19378854611480714	0.001197704240511787
52-53	0.0	5.3231299578301645E-5	0.0	0.22370453647781266	0.001357398139246692
54-55	0.0	5.3231299578301645E-5	0.0	0.25747979606024507	0.0014106294388249938
56-57	0.0	5.3231299578301645E-5	0.0	0.29530063441062837	0.0015170920379815969
58-59	0.0	5.3231299578301645E-5	0.0	0.3369807419804386	0.0015437076877707478
60-61	0.0	5.3231299578301645E-5	0.0	0.38528814634774733	0.0015437076877707478
62-63	0.0	5.3231299578301645E-5	0.0	0.43620388439439284	0.0015437076877707478
64-65	0.0	5.3231299578301645E-5	0.0	0.4899142656688992	0.0015437076877707478
66-67	0.0	5.3231299578301645E-5	0.0	0.5479097665594588	0.0015437076877707478
68-69	0.0	5.3231299578301645E-5	0.0	0.6121067138508907	0.0015437076877707478
70-71	0.0	5.3231299578301645E-5	0.0	0.6889993260917473	0.0015437076877707478
72-73	0.0	5.3231299578301645E-5	0.0	0.7658653226828149	0.0015969389873490493
74-75	0.0	5.3231299578301645E-5	0.0	0.8464308945945744	0.0016235546371382002
76-77	0.0	5.3231299578301645E-5	0.0	0.9377758046709401	0.001650170286927351
78-79	0.0	5.3231299578301645E-5	0.0	1.0406719067557972	0.001650170286927351
80-81	0.0	5.3231299578301645E-5	0.0	1.1547731974018867	0.0016767859367165018
82-83	0.0	5.3231299578301645E-5	0.0	1.2778971933264986	0.001809864185662256
84-85	0.0	5.3231299578301645E-5	0.0	1.4029109003861397	0.001809864185662256
86-87	0.0	5.3231299578301645E-5	0.0	1.540593656745417	0.0018630954852405577
88-89	0.0	5.3231299578301645E-5	0.0	1.693394102184932	0.0019163267848188593
90-91	0.0	5.3231299578301645E-5	0.0	1.869163853392484	0.0019163267848188593
92-93	0.0	5.3231299578301645E-5	0.0	2.058826973789973	0.001969558084397161
94-95	0.0	5.3231299578301645E-5	0.0	2.2566078673731527	0.001969558084397161
96-97	0.0	5.3231299578301645E-5	0.0	2.4564647816398857	0.0020494050337646133
98-99	0.0	5.3231299578301645E-5	0.0	2.6826711891978787	0.002076020683553764
100-101	0.0	5.3231299578301645E-5	0.0	2.937888655026046	0.002076020683553764
102-103	0.0	5.3231299578301645E-5	0.0	3.2132541677446005	0.0021026363333429146
104-105	0.0	5.3231299578301645E-5	0.0	3.5019807366573086	0.0021292519831320657
106-107	0.0	5.3231299578301645E-5	0.0	3.795019040835859	0.0021824832827103675
108-109	0.0	5.3231299578301645E-5	0.0	4.105756752124195	0.0021824832827103675
110-111	0.0	5.3231299578301645E-5	0.0	4.446783072872584	0.002209098932499518
112-113	0.0	7.984694936745247E-5	0.0	4.8244591433806345	0.0022357145822886693
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2065	0.0	71.770294	1
GTATCAA	4200	0.0	57.39462	1
TCAACGC	5150	0.0	45.748814	4
CAACGCA	5255	0.0	44.945534	5
AACGCAG	5550	0.0	42.447075	6
TATCAAC	5680	0.0	41.79313	2
ATCAACG	5710	0.0	41.260967	3
GTACATG	9210	0.0	37.0952	1
ACGCAGA	6525	0.0	35.830894	7
TACATGG	9610	0.0	35.164566	2
ACATGGG	9745	0.0	34.555317	3
CGCAGAG	7010	0.0	33.264347	8
CATGGGG	8270	0.0	31.798647	4
GCAGAGT	7770	0.0	29.781015	9
CAGAGTA	7275	0.0	26.247198	10-11
AGAGTAC	6945	0.0	25.781319	10-11
GTACTAA	300	2.237357E-10	23.808138	1
ATGGGGG	4110	0.0	23.739456	5
ATGGGGC	3445	0.0	23.486507	5
GAGTACT	2860	0.0	21.319094	12-13
>>END_MODULE