FastQCFastQC Report
Mon 15 Aug 2016
SRR2921486_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921486_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1878594
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32840.1748115878151426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31000.1650170286927351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29350.074.72741
GTATCAA56750.057.866321
TCAACGC73750.043.6787454
CAACGCA75200.042.65095
TATCAAC78800.041.2584532
ATCAACG78700.041.1595573
AACGCAG78000.041.10896
ACGCAGA92400.034.7023167
CGCAGAG96700.032.8498428
GTACATG86700.032.5836221
TACATGG89450.030.9541652
GCAGAGT104450.029.784399
ACATGGG93800.029.010813
TGTACGC2751.8189894E-1228.1486242
GTGGTAT11550.027.3485341
CATGGGG78300.026.7679964
AGAGTAC94600.026.75706910-11
CAGAGTA98700.026.73046710-11
TACGCGG1200.001287469324.7939685
GAGTACT57200.024.23268712-13
Produced by FastQC (version 0.11.5)