##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921486_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1878594
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28941059111229	31.0	31.0	34.0	28.0	34.0
2	31.348621894885216	33.0	31.0	34.0	28.0	34.0
3	31.427207262452665	33.0	31.0	34.0	28.0	34.0
4	34.92817234591402	37.0	35.0	37.0	32.0	37.0
5	34.582339771126705	37.0	35.0	37.0	31.0	37.0
6	34.55615795642912	37.0	35.0	37.0	31.0	37.0
7	34.395689542285346	37.0	35.0	37.0	30.0	37.0
8	34.368642186656615	37.0	35.0	37.0	30.0	37.0
9	35.94047782543753	39.0	37.0	39.0	30.0	39.0
10-11	35.81179461874146	39.0	37.0	39.0	30.0	39.0
12-13	35.71891478414176	39.0	37.0	39.0	30.0	39.0
14-15	36.907884566862236	40.0	37.0	41.0	30.0	41.0
16-17	36.891616549398115	40.0	37.0	41.0	30.0	41.0
18-19	36.914387302418724	40.0	37.0	41.0	30.0	41.0
20-21	36.90038667216014	40.0	37.0	41.0	30.0	41.0
22-23	36.84194376219662	40.0	37.0	41.0	30.0	41.0
24-25	36.778008712899116	40.0	37.0	41.0	30.0	41.0
26-27	36.65375408417146	40.0	36.5	41.0	30.0	41.0
28-29	36.472254515877296	40.0	36.0	41.0	29.5	41.0
30-31	36.421600941981076	40.0	36.0	41.0	29.5	41.0
32-33	36.293233929204504	40.0	35.5	41.0	29.0	41.0
34-35	36.166582295056834	40.0	35.0	41.0	29.0	41.0
36-37	36.028838322703045	40.0	35.0	41.0	28.0	41.0
38-39	35.855577628801115	40.0	35.0	41.0	27.5	41.0
40-41	35.65234345473263	40.0	35.0	41.0	27.0	41.0
42-43	35.47580557587217	39.0	35.0	41.0	26.0	41.0
44-45	35.25087379178258	39.0	35.0	41.0	25.5	41.0
46-47	34.95981382885286	39.0	35.0	41.0	24.0	41.0
48-49	34.6931707968832	39.0	34.0	41.0	23.0	41.0
50-51	34.468165287443696	39.0	34.0	41.0	23.0	41.0
52-53	34.25580833325348	38.0	34.0	41.0	22.0	41.0
54-55	34.03171307903677	38.0	33.5	40.0	21.5	41.0
56-57	33.764182148990145	37.5	33.0	40.0	20.5	41.0
58-59	33.60548633712234	37.0	33.0	40.0	20.5	41.0
60-61	33.476294239202296	37.0	33.0	40.0	20.0	41.0
62-63	33.19821526098774	36.0	33.0	40.0	20.0	41.0
64-65	32.89518890191282	36.0	33.0	39.0	19.0	41.0
66-67	32.58555813549921	35.0	33.0	39.0	18.0	41.0
68-69	32.26997983598372	35.0	32.0	38.5	18.0	40.5
70-71	31.966186946194867	35.0	32.0	37.5	17.0	40.0
72-73	31.64841179094578	35.0	32.0	37.0	14.0	39.0
74-75	31.36486382901255	35.0	32.0	36.5	13.0	39.0
76-77	31.084334347921903	35.0	32.0	36.0	11.5	38.5
78-79	30.8120551859529	35.0	31.0	35.5	9.0	37.0
80-81	30.595477522019127	35.0	31.0	35.0	7.0	37.0
82-83	30.41743399585009	35.0	31.0	35.0	7.0	36.5
84-85	30.236955670038334	35.0	31.0	35.0	7.0	36.0
86-87	30.10089087902974	35.0	31.0	35.0	6.5	36.0
88-89	30.0040394571685	35.0	31.0	35.0	3.5	35.5
90-91	29.908236958065448	35.0	31.0	35.0	2.0	35.0
92-93	29.970221612546403	35.0	31.0	35.0	2.0	35.0
94-95	29.941264850201797	35.0	31.0	35.0	2.0	35.0
96-97	29.92656662376224	35.0	31.0	35.0	2.0	35.0
98-99	29.88622554953332	35.0	31.0	35.0	2.0	35.0
100-101	29.83602364321402	35.0	31.0	35.0	2.0	35.0
102-103	28.96404518485633	33.5	29.5	35.0	2.0	35.0
104-105	29.665468696269656	34.5	31.0	35.0	2.0	35.0
106-107	29.772450566753648	35.0	31.0	35.0	2.0	35.0
108-109	29.775788435393704	35.0	31.0	35.0	2.0	35.0
110-111	29.746119970573737	35.0	31.0	35.0	2.0	35.0
112-113	29.69899163949209	35.0	31.0	35.0	2.0	35.0
114-115	29.63626733610349	35.0	31.0	35.0	2.0	35.0
116-117	29.5620676953083	35.0	31.0	35.0	2.0	35.0
118-119	29.468551746678635	35.0	31.0	35.0	2.0	35.0
120-121	29.348839344743993	35.0	31.0	35.0	2.0	35.0
122-123	29.234620146769338	35.0	31.0	35.0	2.0	35.0
124-125	28.331861487899992	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	30506.0
3	11619.0
4	6555.0
5	5263.0
6	5748.0
7	6941.0
8	8117.0
9	8852.0
10	10191.0
11	11022.0
12	11867.0
13	13403.0
14	16971.0
15	14589.0
16	7993.0
17	6595.0
18	6005.0
19	5360.0
20	5131.0
21	5547.0
22	6499.0
23	8711.0
24	12570.0
25	17511.0
26	21287.0
27	23431.0
28	28347.0
29	33766.0
30	38553.0
31	46736.0
32	57528.0
33	74169.0
34	103047.0
35	164905.0
36	335812.0
37	540730.0
38	166266.0
39	451.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.07384922531995	17.362095971749	12.46064776693565	25.1034070359954
2	17.021893862489573	19.516939277522233	37.20285132838239	26.25831553160581
3	19.356618038547545	23.660206580768822	28.94484080502609	28.038334575657544
4	13.67179760319574	15.251025299600531	34.95627163781624	36.12090545938748
5	14.622375284062471	36.831907430243625	33.161617968721714	15.384099316972186
6	34.167070411287106	34.42353254359111	16.334034340476506	15.075362704645281
7	29.801275495400432	31.44656766732559	20.04361323282556	18.708543604448423
8	28.24078411665503	33.17052578232799	18.920242577040355	19.668447523976628
9	27.852280397672224	14.193050743878729	18.560503627300466	39.39416523114858
10-11	26.744139311285064	24.581155030731136	26.378389428202055	22.296316229781745
12-13	27.012248858997452	22.243154982841375	26.802241041596186	23.942355116564986
14-15	24.406853194502112	23.1671936716812	24.52074483375387	27.905208300062817
16-17	24.3123842439168	26.249207441821838	24.95007748570467	24.48833082855669
18-19	24.302595526965383	25.04787061598583	27.04802925311192	23.601504603936863
20-21	25.33851544318246	23.9545609780314	27.042106106547354	23.66481747223879
22-23	25.08831194619539	23.632731970082407	26.889180918178063	24.38977516554414
24-25	24.52117600960842	24.17616612860635	26.90203966029796	24.400618201487262
26-27	24.348121664081155	24.19908667825798	27.01185252696684	24.44093913069402
28-29	24.492284545853106	24.249037914052323	26.996117051194922	24.26256048889964
30-31	24.66331028482925	24.21599680121477	26.790225602740474	24.3304673112155
32-33	24.309882437182484	24.353910264686036	26.75502400368407	24.58118329444741
34-35	24.568134874544747	24.342575508347135	26.8553542330915	24.23393538401662
36-37	24.815640509974966	24.187368816140957	26.6310853671684	24.365905306715682
38-39	24.76522909530011	24.389739401367468	26.552671846765065	24.292359656567356
40-41	24.796170372022676	24.338175911823157	26.511240641671375	24.354413074482792
42-43	24.682052506487516	24.411478717035713	26.387665561746054	24.518803214730713
44-45	24.723842796433754	24.424632562378946	26.21342527570466	24.63809936548264
46-47	24.854987549825985	24.648895509578676	25.86998153104398	24.62613540955136
48-49	25.049788109366926	24.822443928645875	25.387955922914884	24.73981203907232
50-51	24.903145192503754	25.057484665875535	25.35266756001484	24.686702581605864
52-53	25.092489040677606	24.955442604350825	25.260709015648263	24.691359339323302
54-55	24.869601929843522	24.89776588987732	25.45966085055159	24.772971329727568
56-57	24.82166606858203	25.01647838536315	25.345487052088732	24.81636849396609
58-59	24.90917280167301	24.79920587427301	25.429885236161365	24.861736087892616
60-61	24.874583152867064	24.955323783772705	25.57055514574443	24.59953791761581
62-63	24.7824956121097	24.979734375902634	25.514986445658444	24.72278356632922
64-65	24.849015965029363	24.714529899058252	25.597074741722597	24.839379394189788
66-67	24.84146388533599	24.891001846027713	25.524134782649206	24.74339948598709
68-69	24.916926982531372	24.83469254863918	25.38988811964005	24.858492349189394
70-71	24.74319451147396	24.699724780435268	25.64298866246592	24.91409204562485
72-73	25.005883079517936	24.954239757052772	25.289309086882174	24.750568076547115
74-75	24.72649240232271	25.135306298960437	25.29566931995058	24.842531978766274
76-77	24.864335070149668	24.897981782860516	25.25233703571396	24.985346111275856
78-79	24.78114372338675	24.903370980357913	25.326547249048087	24.98893804720725
80-81	24.729775597211702	25.05647006520845	25.15534093011555	25.058413407464297
82-83	24.857998820815926	24.99234062715025	25.18585458238118	24.963805969652647
84-85	24.74962673267511	24.968010411290148	25.089606784268387	25.192756071766347
86-87	24.506571285665725	25.049225082605236	25.229914475994104	25.21428915573493
88-89	24.61709122166901	24.90928253137343	25.242310345185594	25.231315901771968
90-91	24.61259247803193	24.889910115332516	25.24365114836068	25.253846258274876
92-93	24.613274014276286	25.02401607300141	25.215266263652698	25.147443649069608
94-95	24.567318211363677	24.818987265625708	25.364114947411682	25.249579575598936
96-97	24.517105424607337	24.864396128592315	25.305349921734493	25.313148525065856
98-99	24.47380503391565	24.87645440717146	25.18722477219888	25.462515786714008
100-101	24.409876813997602	24.929819807397763	25.27716717446266	25.383136204141977
102-103	24.45794789283656	24.78437290810779	25.374115167025323	25.38356403203032
104-105	24.312586519730292	24.948876670807284	25.251424572894294	25.487112236568134
106-107	24.3694739139461	24.90974613939178	25.219499731181365	25.501280215480758
108-109	24.31705255592011	24.831976786748214	25.224578893776744	25.626391763554928
110-111	24.37975010346925	24.859448172886133	25.267070429620286	25.493731294024336
112-113	24.383580312899937	24.851444931613027	25.22992630007645	25.535048455410585
114-115	24.04894732639536	25.028239865853983	25.36238055947406	25.560432248276598
116-117	24.127422517632287	25.01653629720564	25.26293710723799	25.59310407792409
118-119	24.096856976834253	24.765671395074047	25.300036174432634	25.83743545365906
120-121	23.87214402827758	24.864121755424485	25.42490897089198	25.838825245405957
122-123	23.820241107260102	24.875850574608627	25.341094857134337	25.96281346099693
124-125	23.81341955442085	24.923786717284727	25.29200116606123	25.970792562233193
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	52.0
1	58.5
2	85.0
3	116.5
4	90.0
5	80.5
6	166.5
7	343.5
8	576.5
9	705.0
10	596.5
11	499.5
12	551.5
13	665.0
14	799.0
15	944.0
16	1128.5
17	1277.0
18	1489.0
19	1745.0
20	1947.0
21	2206.5
22	2482.0
23	2910.0
24	3462.5
25	4126.5
26	5173.0
27	6311.5
28	7286.0
29	8614.5
30	10280.5
31	11939.5
32	14061.5
33	16275.5
34	18577.0
35	21195.0
36	24349.0
37	27548.5
38	30221.0
39	33050.5
40	36439.0
41	39752.5
42	43054.5
43	48142.5
44	53252.5
45	56992.0
46	61307.0
47	65828.5
48	71305.5
49	76497.5
50	78035.0
51	78620.0
52	79133.0
53	80091.0
54	81659.0
55	81693.5
56	78842.5
57	73544.0
58	69286.5
59	64427.0
60	57116.5
61	49658.0
62	42282.5
63	33341.5
64	24944.0
65	19252.0
66	14762.5
67	11381.0
68	9253.5
69	7398.0
70	6015.5
71	4894.5
72	4022.5
73	3672.5
74	2977.0
75	2254.5
76	1569.5
77	858.5
78	492.0
79	292.0
80	144.0
81	63.0
82	38.0
83	19.5
84	7.5
85	4.0
86	4.0
87	3.0
88	2.5
89	1.5
90	1.0
91	1.0
92	0.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.5
98	0.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12088828134232303
2	0.0016501702869273509
3	0.020973132033850848
4	0.058235041738662
5	0.0258704115950546
6	0.018311567054935765
7	0.014106294388249936
8	0.005216667358673561
9	0.0033003405738547017
10-11	0.006627296797498555
12-13	0.004923895210992902
14-15	0.002874490177228289
16-17	0.0097413278228292
18-19	0.004950510860782053
20-21	0.011098725962075893
22-23	0.008916242679365525
24-25	0.012881974497948997
26-27	0.00936870872578109
28-29	0.013494134443099468
30-31	0.020281125139332924
32-33	0.01258920235026834
34-35	0.019935121692073964
36-37	0.014186141337617388
38-39	0.020227893839754627
40-41	0.009954253021142407
42-43	0.009501786974726843
44-45	0.01682109066674332
46-47	0.016342008970538606
48-49	0.020467434687856983
50-51	0.014212756987406538
52-53	0.021212672881953207
54-55	0.016342008970538606
56-57	0.02001496864144142
58-59	0.016075852472647097
60-61	0.019030189599242835
62-63	0.022011142375627728
64-65	0.0176994071097853
66-67	0.01258920235026834
68-69	0.022729764919934805
70-71	0.014665223033822103
72-73	0.017353403662526336
74-75	0.018923727000086234
76-77	0.013467518793310316
78-79	0.02847874527439138
80-81	0.020573897287013587
82-83	0.00939532437557024
84-85	0.013227977945207957
86-87	0.012376277151955132
88-89	0.01982865909291736
90-91	0.01290859014773815
92-93	0.00875654878063062
94-95	0.0071862254430707226
96-97	0.0027946432278608364
98-99	0.0019429424346080102
100-101	0.011444729409334853
102-103	0.0032737249240655515
104-105	0.0025817180295476297
106-107	2.1292519831320655E-4
108-109	0.01897695829966454
110-111	8.783164430419771E-4
112-113	0.008836395729998073
114-115	0.0023421771814452724
116-117	0.008011310586534398
118-119	0.010406719067557971
120-121	0.003939116168794322
122-123	5.855442953613181E-4
124-125	0.025364714249060734
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1878594.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.75842184180435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.74693872571792	46.0605732751254
2	10.673636411187237	12.329847888467905
3	3.552006095140739	6.154747992833971
4	1.7116056464349907	3.954385638144257
5	1.0133757172566493	2.92654910807753
6	0.6472569877196582	2.243072528206056
7	0.46214276969753637	1.86848459303311
8	0.34536796775698575	1.595832701788373
9	0.26337893672663926	1.3691116558532814
>10	1.4814992473031219	15.393276255369956
>50	0.07667755864750292	2.9983594029694256
>100	0.025375986973707604	2.494867752161275
>500	4.6121839838888666E-4	0.182798511646909
>1k	2.76731039033332E-4	0.4280926963226214
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3284	0.1748115878151426	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3100	0.1650170286927351	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.3231299578301645E-5
2	0.0	0.0	0.0	0.0	5.3231299578301645E-5
3	0.0	0.0	0.0	0.0	5.3231299578301645E-5
4	0.0	0.0	0.0	0.0	5.3231299578301645E-5
5	0.0	0.0	0.0	0.0	5.3231299578301645E-5
6	0.0	0.0	0.0	0.0	5.3231299578301645E-5
7	0.0	0.0	0.0	0.0	5.3231299578301645E-5
8	0.0	0.0	0.0	0.0	5.3231299578301645E-5
9	0.0	0.0	0.0	0.0	5.3231299578301645E-5
10-11	0.0	0.0	0.0	0.0	5.3231299578301645E-5
12-13	0.0	0.0	0.0	0.0	1.0646259915660329E-4
14-15	0.0	0.0	0.0	2.6615649789150823E-5	1.8630954852405575E-4
16-17	0.0	0.0	0.0	7.984694936745247E-5	3.193877974698099E-4
18-19	0.0	0.0	0.0	1.0646259915660329E-4	3.193877974698099E-4
20-21	0.0	0.0	0.0	1.0646259915660329E-4	3.726190970481115E-4
22-23	0.0	0.0	0.0	1.5969389873490494E-4	3.9923474683726233E-4
24-25	0.0	0.0	0.0	3.193877974698099E-4	4.2585039662641316E-4
26-27	0.0	0.0	0.0	5.323129957830164E-4	4.52466046415564E-4
28-29	0.0	0.0	0.0	0.0015703233375598986	4.790816962047148E-4
30-31	0.0	0.0	0.0	0.004072194417740076	4.790816962047148E-4
32-33	0.0	0.0	0.0	0.009475171324937692	4.790816962047148E-4
34-35	0.0	0.0	0.0	0.017300172362948035	5.323129957830164E-4
36-37	0.0	0.0	0.0	0.02877151742207204	5.323129957830164E-4
38-39	0.0	0.0	0.0	0.04766862877236912	7.186225443070722E-4
40-41	0.0	0.0	0.0	0.06632619927456385	7.45238194096223E-4
42-43	0.0	0.0	0.0	0.08628793661642697	7.45238194096223E-4
44-45	0.0	0.0	0.0	0.10933708933383157	7.45238194096223E-4
46-47	0.0	0.0	0.0	0.13640520516939797	8.250851434636755E-4
48-49	0.0	0.0	0.0	0.16656073638050584	9.04932092831128E-4
50-51	0.0	0.0	0.0	0.1974348901359208	9.04932092831128E-4
52-53	0.0	0.0	0.0	0.2286284316888056	9.315477426202789E-4
54-55	0.0	0.0	0.0	0.2631489294653342	9.581633924094297E-4
56-57	0.0	0.0	0.0	0.3020876251068618	9.847790421985804E-4
58-59	0.0	0.0	0.0	0.3451251308159187	0.0010113946919877313
60-61	0.0	0.0	0.0	0.39428423597648027	0.0010113946919877313
62-63	0.0	0.0	0.0	0.4465041408627942	0.0011178572911443346
64-65	0.0	0.0	0.0	0.5009863759811859	0.0011444729409334853
66-67	0.0	0.0	0.0	0.5606054315088838	0.0011710885907226362
68-69	0.0	0.0	0.0	0.6261863925893514	0.0011710885907226362
70-71	0.0	0.0	0.0	0.704117015171985	0.0012509355400900886
72-73	0.0	0.0	0.0	0.7833251889444979	0.0014372450886141444
74-75	0.0	0.0	0.0	0.8647158459997211	0.0015437076877707478
76-77	0.0	0.0	0.0	0.9572052290170201	0.0015437076877707478
78-79	0.0	0.0	0.0	1.0613256509921782	0.0015437076877707478
80-81	0.0	0.0	0.0	1.1774231153724541	0.0015969389873490493
82-83	0.0	0.0	0.0	1.302356975482728	0.0018630954852405575
84-85	0.0	0.0	0.0	1.4295531658250797	0.0018630954852405575
86-87	0.0	0.0	0.0	1.5686997829227602	0.001916326784818859
88-89	0.0	0.0	0.0	1.7236028646956183	0.001969558084397161
90-91	0.0	0.0	0.0	1.902167259131031	0.001969558084397161
92-93	0.0	0.0	0.0	2.095982420895627	0.002076020683553764
94-95	0.0	0.0	0.0	2.298287974942963	0.0021292519831320657
96-97	0.0	0.0	0.0	2.503095400070478	0.0022357145822886693
98-99	0.0	0.0	0.0	2.734944325383771	0.0022889458818669706
100-101	0.0	0.0	0.0	2.9944735264777806	0.0022889458818669706
102-103	0.0	0.0	0.0	3.2742838527111235	0.002395408481023574
104-105	0.0	0.0	0.0	3.567348772539463	0.0024486397806018755
106-107	0.0	0.0	0.0	3.866269135321416	0.0024486397806018755
108-109	0.0	0.0	0.0	4.184246303352401	0.0024752554303910266
110-111	0.0	0.0	0.0	4.5341090198307885	0.0025817180295476297
112-113	0.0	0.0	0.0	4.921579649461246	0.0026083336793367804
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2935	0.0	74.7274	1
GTATCAA	5675	0.0	57.86632	1
TCAACGC	7375	0.0	43.678745	4
CAACGCA	7520	0.0	42.6509	5
TATCAAC	7880	0.0	41.258453	2
ATCAACG	7870	0.0	41.159557	3
AACGCAG	7800	0.0	41.1089	6
ACGCAGA	9240	0.0	34.702316	7
CGCAGAG	9670	0.0	32.849842	8
GTACATG	8670	0.0	32.583622	1
TACATGG	8945	0.0	30.954165	2
GCAGAGT	10445	0.0	29.78439	9
ACATGGG	9380	0.0	29.01081	3
TGTACGC	275	1.8189894E-12	28.148624	2
GTGGTAT	1155	0.0	27.348534	1
CATGGGG	7830	0.0	26.767996	4
AGAGTAC	9460	0.0	26.757069	10-11
CAGAGTA	9870	0.0	26.730467	10-11
TACGCGG	120	0.0012874693	24.793968	5
GAGTACT	5720	0.0	24.232687	12-13
>>END_MODULE