FastQCFastQC Report
Mon 15 Aug 2016
SRR2921485_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921485_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1870311
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21100.070.7923051
GTATCAA42250.061.130481
TCAACGC54650.046.3789864
CAACGCA55900.045.341895
AACGCAG57100.044.2788736
ATCAACG59150.043.1511883
TATCAAC62100.041.292952
GTACATG100950.039.3200721
TACATGG104600.037.0856672
ACGCAGA69100.036.6754387
ACATGGG106900.036.1764453
CGCAGAG74650.033.9469158
CATGGGG84000.032.936354
GCAGAGT78250.031.0175849
ATGGGGG44950.028.7231375
AGAGTAC72350.026.51693510-11
CAGAGTA75900.025.4334410-11
ATGGGGA33750.023.4465925
CATGGGT18500.022.8343414
GTATAGA5850.022.380081
Produced by FastQC (version 0.11.5)