##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921485_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1870311
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.08700852425078	31.0	30.0	34.0	26.0	34.0
2	30.111610315075943	31.0	30.0	34.0	26.0	34.0
3	30.259944469128396	31.0	31.0	34.0	26.0	34.0
4	33.640025642794164	37.0	35.0	37.0	28.0	37.0
5	33.682299360908424	37.0	35.0	37.0	28.0	37.0
6	33.68183098960547	37.0	35.0	37.0	28.0	37.0
7	33.556923955427735	37.0	35.0	37.0	27.0	37.0
8	33.466459321471135	37.0	35.0	37.0	27.0	37.0
9	34.94103547484884	39.0	35.0	39.0	27.0	39.0
10-11	34.836999835856176	39.0	35.0	39.0	27.0	39.0
12-13	34.687787485610684	39.0	35.0	39.0	26.5	39.0
14-15	35.868003770495925	40.0	36.0	41.0	26.5	41.0
16-17	35.802043350009704	40.0	36.0	41.0	26.0	41.0
18-19	35.82179354128805	40.0	36.0	41.0	26.0	41.0
20-21	35.85529706022153	40.0	36.0	41.0	26.0	41.0
22-23	35.82897550193524	40.0	36.0	41.0	26.0	41.0
24-25	35.795155458102954	40.0	36.0	41.0	26.0	41.0
26-27	35.72277765569469	40.0	36.0	41.0	26.0	41.0
28-29	35.68744021716174	40.0	35.0	41.0	26.0	41.0
30-31	35.60104201921499	40.0	35.0	41.0	26.0	41.0
32-33	35.49500965347474	40.0	35.0	41.0	25.5	41.0
34-35	35.328338442109356	40.0	35.0	41.0	25.0	41.0
36-37	35.19039133063966	40.0	35.0	41.0	25.0	41.0
38-39	35.05826624556023	39.5	35.0	41.0	24.0	41.0
40-41	34.90590147841722	39.0	34.5	41.0	24.0	41.0
42-43	34.70930823804169	39.0	34.0	41.0	23.0	41.0
44-45	34.50400227555738	39.0	34.0	41.0	22.5	41.0
46-47	34.243749568921956	39.0	33.0	41.0	21.0	41.0
48-49	34.08951773261238	39.0	33.0	41.0	20.0	41.0
50-51	33.887883084684844	38.0	33.0	40.5	18.0	41.0
52-53	33.69970261630285	38.0	33.0	40.0	18.0	41.0
54-55	33.49356550862397	38.0	33.0	40.0	17.5	41.0
56-57	33.247751309808905	37.0	33.0	40.0	15.0	41.0
58-59	33.00665023089743	37.0	32.5	40.0	14.0	41.0
60-61	32.69846164621819	36.0	32.0	40.0	12.0	41.0
62-63	32.37683545677697	36.0	32.0	39.0	8.5	41.0
64-65	32.06558107181105	35.0	31.5	39.0	8.0	41.0
66-67	32.01690013051305	35.0	32.0	39.0	7.5	41.0
68-69	31.765713563145383	35.0	32.0	38.5	7.0	40.5
70-71	31.46306977823474	35.0	31.5	37.0	3.5	40.0
72-73	31.132993122534167	35.0	31.0	37.0	2.0	39.0
74-75	30.829562035404805	35.0	31.0	36.5	2.0	39.0
76-77	30.547648225348617	35.0	31.0	36.0	2.0	38.5
78-79	30.27569131550849	35.0	31.0	35.5	2.0	37.0
80-81	30.03702218508045	35.0	31.0	35.0	2.0	37.0
82-83	29.841691568942277	35.0	30.5	35.0	2.0	36.5
84-85	29.643806030120125	35.0	30.0	35.0	2.0	36.0
86-87	29.491715281576166	35.0	30.0	35.0	2.0	36.0
88-89	29.373948503751514	35.0	30.0	35.0	2.0	35.5
90-91	29.261611838886687	34.0	30.0	35.0	2.0	35.0
92-93	29.16349660564473	34.0	30.0	35.0	2.0	35.0
94-95	29.10011463334173	34.0	30.0	35.0	2.0	35.0
96-97	28.998486347992394	34.0	30.0	35.0	2.0	35.0
98-99	28.937389557137823	34.0	30.0	35.0	2.0	35.0
100-101	28.86912550907309	34.0	30.0	35.0	2.0	35.0
102-103	27.881569161492394	33.5	28.0	34.5	2.0	35.0
104-105	28.7052107911465	34.0	29.5	35.0	2.0	35.0
106-107	29.032630402109596	35.0	30.0	35.0	2.0	35.0
108-109	29.071227993633144	35.0	30.0	35.0	2.0	35.0
110-111	29.013102901068322	35.0	30.0	35.0	2.0	35.0
112-113	28.939013885925924	35.0	30.0	35.0	2.0	35.0
114-115	28.81714297782561	35.0	30.0	35.0	2.0	35.0
116-117	28.75078743588633	35.0	30.0	35.0	2.0	35.0
118-119	28.592103398846504	35.0	30.0	35.0	2.0	35.0
120-121	28.421990781212322	35.0	30.0	35.0	2.0	35.0
122-123	28.295192083027903	35.0	30.0	35.0	2.0	35.0
124-125	27.44004740388096	34.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61962.0
3	11579.0
4	7728.0
5	6705.0
6	7003.0
7	8112.0
8	9030.0
9	9743.0
10	10566.0
11	11181.0
12	11416.0
13	11818.0
14	13508.0
15	11684.0
16	8357.0
17	7130.0
18	6597.0
19	5988.0
20	5889.0
21	6135.0
22	7235.0
23	10053.0
24	14330.0
25	20388.0
26	23698.0
27	25047.0
28	29625.0
29	34994.0
30	39298.0
31	46907.0
32	59519.0
33	77447.0
34	108625.0
35	173216.0
36	331316.0
37	495513.0
38	140841.0
39	128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.56184249444151	17.209911315473768	12.26596693197323	24.962279258111497
2	16.77181764750239	19.562039970029698	38.098918042132766	25.567224340335137
3	18.925392962060332	24.28676938150224	29.06001967016376	27.727817986273667
4	13.396649634232304	15.190724400807373	35.72607481363978	35.686551151320536
5	14.325735578447885	37.35138809628612	33.33966227010583	14.98321405516016
6	33.300846494336646	34.652402412441994	17.384559836317685	14.662191256903675
7	29.602772807869126	31.383649312547973	20.743961895286922	18.26961598429598
8	27.810918317735915	33.294787732657554	19.600972803957113	19.29332114564942
9	27.903361065888525	14.540391193754933	18.89874871908968	38.65749902126686
10-11	26.287665756069643	24.865610053205074	26.915991073207458	21.93073311751783
12-13	26.521316129529154	22.716675295668097	26.94887958478101	23.813128990021738
14-15	24.117219334167526	23.74651309626355	24.294736864625367	27.841530704943562
16-17	24.17389690455872	26.691712533425616	24.846139877992325	24.28825068402334
18-19	24.259740648781918	25.850575760987976	26.389974727124354	23.49970886310575
20-21	25.491917993338376	24.793870578756632	26.004620123752552	23.70959130415244
22-23	25.35212134083484	24.423143689687066	25.8270016845761	24.397733284901992
24-25	24.64463825556085	24.91396336183432	25.895844373531556	24.54555400907327
26-27	24.35860574128869	24.938196187220285	26.050223504732877	24.65297456675815
28-29	24.424844656704153	25.010457856176927	26.06036242489601	24.504335062222907
30-31	24.68202585517486	24.914531994802648	25.911074759831028	24.492367390191465
32-33	24.34057514157329	25.01800844879114	25.864438977639033	24.77697743199654
34-35	24.640321824915436	25.04257622535018	25.978852025152065	24.338249924582314
36-37	24.79207316991624	24.790521826466186	25.824358499422928	24.593046504194646
38-39	24.746956645251927	25.101494198974905	25.712345230876068	24.4392039248971
40-41	24.706768123888363	24.936491167070088	25.909517632427082	24.447223076614467
42-43	24.600309699964452	24.978246372768233	25.722965383846862	24.698478543420453
44-45	24.55733156718909	25.013213983068983	25.698709416487624	24.730745033254298
46-47	24.652597118589796	25.05433708224648	25.639987170429713	24.65307862873401
48-49	24.715405012594875	25.257126442499807	25.343142840178	24.684325704727318
50-51	24.69063057173899	25.38586591076017	25.274330829633772	24.64917268786707
52-53	24.845548897924246	25.2642574363364	25.379199657607533	24.51099400813182
54-55	24.691500668912244	25.155058725734214	25.494088377723322	24.659352227630222
56-57	24.59009509820467	25.26800779999449	25.322594923691693	24.819302178109144
58-59	24.73873485459178	25.113548771544313	25.34147543061877	24.80624094324514
60-61	24.72069425765525	25.235835748407283	25.491039779256404	24.552430214681063
62-63	24.541029958620218	25.36964191724899	25.50612138354822	24.58320674058257
64-65	24.598155316203044	25.021308780340775	25.553847762638238	24.826688140817943
66-67	24.485631099585454	25.299689165673385	25.46089619663193	24.753783538109232
68-69	24.611996635882406	25.283777224816916	25.374806289134945	24.729419850165733
70-71	24.584995291286297	25.095023474027723	25.57750280637197	24.742478428314012
72-73	24.777025913985167	25.370348778750234	25.30317308864838	24.549452218616224
74-75	24.558837963296025	25.533522377625555	25.28335917247702	24.6242804866014
76-77	24.60365511229014	25.2106208984281	25.37053874665055	24.81518524263121
78-79	24.513283826242574	25.272309947298986	25.4085886039915	24.80581762246694
80-81	24.531034447719545	25.480397051395222	25.252916904098683	24.73565159678656
82-83	24.688906019256425	25.415761239397245	25.221426934659767	24.673905806686566
84-85	24.49113667088781	25.450760233417757	25.217759600246424	24.84034349544801
86-87	24.319145061975146	25.542391149440625	25.327549681588103	24.810914106996133
88-89	24.5127056837255	25.330801279111647	25.26579750750244	24.890695529660412
90-91	24.488952344284737	25.263598348733744	25.325012833675565	24.922436473305957
92-93	24.506971979724586	25.445794124386783	25.27720668392382	24.770027211964816
94-95	24.465151671929924	25.268637373702536	25.34507960928098	24.92113134508656
96-97	24.486478956913345	25.334186049060918	25.233469698334876	24.945865295690854
98-99	24.34622213702231	25.399047062167153	25.176867796385405	25.07786300442514
100-101	24.36517483141958	25.45851120915095	25.312396145342912	24.863917814086562
102-103	24.404299449308173	25.247207106432327	25.4437108897317	24.90478255452779
104-105	24.248638974820466	25.414563604383407	25.3497573374249	24.98704008337123
106-107	24.269015854343234	25.409753228350667	25.334999866320885	24.986231050985218
108-109	24.297689346481455	25.438952513720025	25.268638671027983	24.994719468770537
110-111	24.30474477387245	25.568543806539907	25.265206372920034	24.861505046667606
112-113	24.248195884006144	25.580696493577047	25.368309157252185	24.802798465164628
114-115	24.074842474045113	25.828642132685403	25.28919861745041	24.80731677581907
116-117	24.1341723987475	25.85382338346765	25.144932883322213	24.86707133446264
118-119	24.309876721539574	25.50283788886474	25.22107233313618	24.966213056459505
120-121	24.031304969364726	25.64240707729062	25.40541164172665	24.92087631161801
122-123	24.060614034524733	25.783588236175024	25.307878348340836	24.84791938095941
124-125	24.043324909283058	25.796842705523705	25.321422565707458	24.838409819485783
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	140.0
1	166.5
2	210.0
3	225.0
4	208.5
5	189.0
6	187.5
7	238.0
8	310.0
9	351.5
10	389.0
11	451.0
12	524.0
13	596.5
14	713.0
15	797.0
16	854.0
17	975.5
18	1119.0
19	1227.0
20	1392.0
21	1651.0
22	1962.5
23	2296.0
24	2707.0
25	3454.5
26	4718.5
27	6208.0
28	7214.0
29	8637.5
30	11021.0
31	13191.0
32	16005.0
33	19137.0
34	22206.5
35	25401.0
36	28576.0
37	31997.0
38	34457.5
39	36907.0
40	39460.5
41	41597.0
42	44398.0
43	49739.5
44	55379.0
45	59238.0
46	63282.5
47	66795.5
48	70919.0
49	75467.5
50	76207.0
51	75593.0
52	75137.0
53	74932.0
54	76258.0
55	76781.5
56	73635.0
57	67790.0
58	63895.0
59	59731.0
60	53387.5
61	47204.5
62	40983.0
63	32526.5
64	24655.0
65	19682.0
66	15554.0
67	12173.5
68	9903.5
69	8137.5
70	6777.0
71	5427.5
72	4250.5
73	3847.0
74	3202.5
75	2583.0
76	1913.0
77	1141.0
78	683.0
79	403.5
80	243.0
81	127.5
82	70.5
83	36.5
84	27.0
85	21.0
86	16.0
87	14.5
88	13.0
89	12.5
90	9.5
91	7.0
92	8.5
93	12.0
94	9.0
95	6.5
96	6.0
97	6.0
98	8.5
99	9.0
100	19.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03475357841556832
2	0.024594840109479117
3	0.025343378721506746
4	0.02913953882536113
5	0.0316524898800253
6	0.04469844854679249
7	0.038817073738004
8	0.035502117027595946
9	0.029246472912793645
10-11	0.03520804828715652
12-13	0.034593177284419546
14-15	0.03403177332539883
16-17	0.036063520986616665
18-19	0.048949078522235075
20-21	0.058653346956736074
22-23	0.05336010962882643
24-25	0.06386638371907132
26-27	0.04745200129817982
28-29	0.04828074047578183
30-31	0.04801340525720054
32-33	0.05611366238021377
34-35	0.03865667260685523
36-37	0.0515154966206155
38-39	0.05964248726548686
40-41	0.04737180073260543
42-43	0.05787807482285031
44-45	0.057690940169843406
46-47	0.06370598258792254
48-49	0.048120339344633056
50-51	0.05047288926814845
52-53	0.05940188556876369
54-55	0.04587472350855018
56-57	0.04397664345662299
58-59	0.04544698715882011
60-61	0.03371097106310127
62-63	0.042720167929290905
64-65	0.04151715944567508
66-67	0.035582317593170334
68-69	0.030930684789855802
70-71	0.04836094104135623
72-73	0.031385154661444
74-75	0.039111142478443425
76-77	0.03213369327347163
78-79	0.03274856427620861
80-81	0.02553051337451365
82-83	0.018045127254237396
84-85	0.019194668694136965
86-87	0.017510456817074808
88-89	0.022429424838970632
90-91	0.01235088709845582
92-93	0.019328336303427614
94-95	0.014516302368964307
96-97	0.011094411571123734
98-99	0.010666675221393663
100-101	0.011201345658556251
102-103	0.006309111158518556
104-105	0.006496245811525463
106-107	0.008608194038317692
108-109	0.012511288229604596
110-111	0.0062289105929441676
112-113	0.00764578725142503
114-115	0.00764578725142503
116-117	0.007218050901694959
118-119	0.24463845852374286
120-121	0.3621322870902219
122-123	0.030957418311713936
124-125	0.003769426581996256
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1870311.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.144978102600284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.0778026475312	42.27520914649723
2	11.019909215349056	11.933454863154354
3	3.8550870568135704	6.262008128243657
4	1.9196903625112343	4.157663705677743
5	1.1679929748316398	3.1620477023125053
6	0.7734093428263974	2.512573915900906
7	0.5533818153849096	2.097399239347519
8	0.40500913716619386	1.7543368690573282
9	0.311876491094586	1.5197891202928955
>10	1.7860680543489251	17.396242461662005
>50	0.09485382127298342	3.464644120284631
>100	0.034123408759990856	3.076518922682426
>500	4.972950683695442E-4	0.16871850766064894
>1k	2.983770410217265E-4	0.21939329722613582
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.0693408743251791E-4
7	0.0	0.0	0.0	0.0	1.0693408743251791E-4
8	0.0	0.0	0.0	0.0	1.0693408743251791E-4
9	0.0	0.0	0.0	5.3467043716258954E-5	1.0693408743251791E-4
10-11	0.0	0.0	0.0	5.3467043716258954E-5	1.0693408743251791E-4
12-13	0.0	0.0	0.0	5.3467043716258954E-5	1.0693408743251791E-4
14-15	0.0	0.0	0.0	5.3467043716258954E-5	1.0693408743251791E-4
16-17	0.0	0.0	0.0	5.3467043716258954E-5	1.6040113114877685E-4
18-19	0.0	0.0	0.0	1.0693408743251791E-4	1.6040113114877685E-4
20-21	0.0	0.0	0.0	1.0693408743251791E-4	1.6040113114877685E-4
22-23	0.0	0.0	0.0	1.8713465300690635E-4	1.6040113114877685E-4
24-25	0.0	0.0	0.0	2.673352185812948E-4	1.8713465300690635E-4
26-27	0.0	0.0	0.0	4.8120339344633056E-4	2.673352185812948E-4
28-29	0.0	0.0	0.0	0.0014970772240552508	2.673352185812948E-4
30-31	0.0	0.0	0.0	0.004999168587470212	2.673352185812948E-4
32-33	0.0	0.0	0.0	0.011736016095718841	2.673352185812948E-4
34-35	0.0	0.0	0.0	0.021306616920929194	3.742693060138127E-4
36-37	0.0	0.0	0.0	0.03576945224617724	4.544698715882011E-4
38-39	0.0	0.0	0.0	0.0584929458255873	4.8120339344633056E-4
40-41	0.0	0.0	0.0	0.08632254207990009	4.8120339344633056E-4
42-43	0.0	0.0	0.0	0.1130025968943133	4.8120339344633056E-4
44-45	0.0	0.0	0.0	0.14345207829072276	5.079369153044601E-4
46-47	0.0	0.0	0.0	0.177777920356561	5.346704371625896E-4
48-49	0.0	0.0	0.0	0.21606032365740244	5.881374808788485E-4
50-51	0.0	0.0	0.0	0.25632100757574544	5.881374808788485E-4
52-53	0.0	0.0	5.3467043716258954E-5	0.297116361931251	6.950715683113664E-4
54-55	0.0	0.0	5.3467043716258954E-5	0.34237621443706423	7.752721338857548E-4
56-57	0.0	0.0	5.3467043716258954E-5	0.39391844457953784	8.020056557438843E-4
58-59	0.0	0.0	5.3467043716258954E-5	0.4514489836182325	8.020056557438843E-4
60-61	0.0	0.0	5.3467043716258954E-5	0.508551786307197	8.020056557438843E-4
62-63	0.0	0.0	5.3467043716258954E-5	0.5696913507967392	9.089397431764022E-4
64-65	0.0	0.0	5.3467043716258954E-5	0.6371934934885162	9.089397431764022E-4
66-67	0.0	0.0	5.3467043716258954E-5	0.7106037445109397	9.089397431764022E-4
68-69	0.0	0.0	5.3467043716258954E-5	0.7983431632493205	9.089397431764022E-4
70-71	0.0	0.0	5.3467043716258954E-5	0.8978987986489948	9.624067868926611E-4
72-73	0.0	0.0	5.3467043716258954E-5	1.001838731633402	0.001069340874325179
74-75	0.0	0.0	5.3467043716258954E-5	1.1056182634866607	0.001069340874325179
76-77	0.0	0.0	5.3467043716258954E-5	1.2234596278372956	0.001069340874325179
78-79	0.0	0.0	5.3467043716258954E-5	1.3572876382590917	0.001069340874325179
80-81	0.0	0.0	5.3467043716258954E-5	1.507449830536205	0.001069340874325179
82-83	0.0	0.0	5.3467043716258954E-5	1.6661132827642033	0.001122807918041438
84-85	0.0	0.0	5.3467043716258954E-5	1.8312729808037274	0.001122807918041438
86-87	0.0	0.0	5.3467043716258954E-5	2.003811130876095	0.001229742005473956
88-89	0.0	0.0	5.3467043716258954E-5	2.2017193931918273	0.0012564755273320855
90-91	0.0	0.0	5.3467043716258954E-5	2.4272701171088658	0.0012832090491902148
92-93	0.0	0.0	5.3467043716258954E-5	2.6645033900779067	0.0012832090491902148
94-95	0.0	0.0	5.3467043716258954E-5	2.905506089628944	0.0014436101803389917
96-97	0.0	0.0	5.3467043716258954E-5	3.161372627333101	0.0014703437021971212
98-99	0.0	0.0	5.3467043716258954E-5	3.444346956201402	0.0014970772240552508
100-101	0.0	0.0	5.3467043716258954E-5	3.7615401930481083	0.0014970772240552508
102-103	0.0	0.0	5.3467043716258954E-5	4.100948986558921	0.0014970772240552508
104-105	0.0	0.0	5.3467043716258954E-5	4.457333566449645	0.0014970772240552508
106-107	0.0	0.0	5.3467043716258954E-5	4.817808375184661	0.0015772777896296392
108-109	0.0	0.0	5.3467043716258954E-5	5.205658310302404	0.0017644124426365454
110-111	0.0	0.0	5.3467043716258954E-5	5.625829073346625	0.001898080051927193
112-113	0.0	0.0	5.3467043716258954E-5	6.085458514653446	0.0019248135737853223
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2110	0.0	70.792305	1
GTATCAA	4225	0.0	61.13048	1
TCAACGC	5465	0.0	46.378986	4
CAACGCA	5590	0.0	45.34189	5
AACGCAG	5710	0.0	44.278873	6
ATCAACG	5915	0.0	43.151188	3
TATCAAC	6210	0.0	41.29295	2
GTACATG	10095	0.0	39.320072	1
TACATGG	10460	0.0	37.085667	2
ACGCAGA	6910	0.0	36.675438	7
ACATGGG	10690	0.0	36.176445	3
CGCAGAG	7465	0.0	33.946915	8
CATGGGG	8400	0.0	32.93635	4
GCAGAGT	7825	0.0	31.017584	9
ATGGGGG	4495	0.0	28.723137	5
AGAGTAC	7235	0.0	26.516935	10-11
CAGAGTA	7590	0.0	25.43344	10-11
ATGGGGA	3375	0.0	23.446592	5
CATGGGT	1850	0.0	22.834341	4
GTATAGA	585	0.0	22.38008	1
>>END_MODULE