FastQCFastQC Report
Mon 15 Aug 2016
SRR2921485_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921485_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1870311
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35750.19114468128562576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31710.16954399562425715No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30100.085.566121
GTATCAA62750.060.996641
TCAACGC82900.046.033264
CAACGCA84750.044.937725
ATCAACG86800.043.9661253
AACGCAG87600.043.7380686
TATCAAC90500.042.3024442
GTACATG97850.037.7150231
ACGCAGA102900.037.0602957
TACATGG102550.035.4158172
CGCAGAG108800.035.050598
ACATGGG103100.034.7052883
GCAGAGT114650.032.847019
CATGGGG86750.031.1569274
ATGGGGG46150.029.012265
CAGAGTA111650.028.34778610-11
AGAGTAC107150.027.7338110-11
GAGTACT63850.026.74158912-13
GTGGTAT11450.025.5138021
TATACCG2109.806536E-825.5031595
Produced by FastQC (version 0.11.5)