##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921485_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1870311 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.331127283109602 33.0 31.0 34.0 28.0 34.0 2 31.391989888312693 33.0 31.0 34.0 28.0 34.0 3 31.465730030994845 33.0 31.0 34.0 28.0 34.0 4 34.9831798027173 37.0 35.0 37.0 32.0 37.0 5 34.65186912764776 37.0 35.0 37.0 31.0 37.0 6 34.62606058564592 37.0 35.0 37.0 31.0 37.0 7 34.47355332883141 37.0 35.0 37.0 30.0 37.0 8 34.449107661773894 37.0 35.0 37.0 30.0 37.0 9 36.026827089184636 39.0 37.0 39.0 30.0 39.0 10-11 35.90197432405627 39.0 37.0 39.0 30.0 39.0 12-13 35.807618358658 39.0 37.0 39.0 30.0 39.0 14-15 36.98950816201156 40.0 37.0 41.0 30.0 41.0 16-17 36.97175095478774 40.0 37.0 41.0 30.0 41.0 18-19 36.99796531165138 40.0 37.0 41.0 30.0 41.0 20-21 36.98498351343707 40.0 37.0 41.0 30.0 41.0 22-23 36.92107195006606 40.0 37.0 41.0 30.0 41.0 24-25 36.85309983206002 40.0 37.0 41.0 30.0 41.0 26-27 36.72672165217442 40.0 36.5 41.0 30.0 41.0 28-29 36.53742746527182 40.0 36.0 41.0 29.5 41.0 30-31 36.47939326668131 40.0 36.0 41.0 30.0 41.0 32-33 36.34132612169847 40.0 35.5 41.0 29.5 41.0 34-35 36.20746683305611 40.0 35.0 41.0 29.0 41.0 36-37 36.06242571422614 40.0 35.0 41.0 28.0 41.0 38-39 35.885659925007126 40.0 35.0 41.0 27.5 41.0 40-41 35.67812919883377 40.0 35.0 41.0 27.0 41.0 42-43 35.49612925337016 39.0 35.0 41.0 26.0 41.0 44-45 35.25783733293554 39.0 35.0 41.0 25.5 41.0 46-47 34.94356072332356 39.0 35.0 41.0 24.0 41.0 48-49 34.653694225185006 39.0 34.0 41.0 23.0 41.0 50-51 34.42582837827506 39.0 34.0 41.0 22.5 41.0 52-53 34.224289436355775 38.0 34.0 41.0 22.0 41.0 54-55 34.00779415829774 38.0 33.5 40.0 21.5 41.0 56-57 33.74619434949589 37.5 33.0 40.0 20.0 41.0 58-59 33.59940405633074 37.0 33.0 40.0 20.5 41.0 60-61 33.47650444230933 37.0 33.0 40.0 20.0 41.0 62-63 33.20838566420237 36.0 33.0 40.0 20.0 41.0 64-65 32.915990976901696 36.0 33.0 39.0 19.5 41.0 66-67 32.61151166838029 35.0 33.0 39.0 18.0 41.0 68-69 32.293390778325104 35.0 32.5 39.0 18.0 41.0 70-71 31.992216535111005 35.0 32.0 37.5 17.0 40.0 72-73 31.683091207825864 35.0 32.0 37.0 14.0 39.0 74-75 31.395856624914252 35.0 32.0 36.5 13.0 39.0 76-77 31.110855627753885 35.0 32.0 36.0 11.5 39.0 78-79 30.842539556255616 35.0 31.5 35.5 9.0 37.0 80-81 30.62114375630577 35.0 31.0 35.0 7.5 37.0 82-83 30.439952232543142 35.0 31.0 35.0 7.0 36.5 84-85 30.25399305249234 35.0 31.0 35.0 7.0 36.0 86-87 30.115804537320265 35.0 31.0 35.0 6.0 36.0 88-89 30.011141729904814 35.0 31.0 35.0 3.5 36.0 90-91 29.916614669966652 35.0 31.0 35.0 2.0 35.0 92-93 29.975840114291152 35.0 31.0 35.0 2.0 35.0 94-95 29.950538974534183 35.0 31.0 35.0 2.0 35.0 96-97 29.93030116381714 35.0 31.0 35.0 2.0 35.0 98-99 29.891403087507907 35.0 31.0 35.0 2.0 35.0 100-101 29.844422932870522 35.0 31.0 35.0 2.0 35.0 102-103 28.972085925816614 33.5 29.5 35.0 2.0 35.0 104-105 29.67301213541491 34.5 31.0 35.0 2.0 35.0 106-107 29.77973315667822 35.0 31.0 35.0 2.0 35.0 108-109 29.785869569285538 35.0 31.0 35.0 2.0 35.0 110-111 29.752490628563912 35.0 31.0 35.0 2.0 35.0 112-113 29.70392811676775 35.0 31.0 35.0 2.0 35.0 114-115 29.64318661441867 35.0 31.0 35.0 2.0 35.0 116-117 29.565215089896814 35.0 31.0 35.0 2.0 35.0 118-119 29.47176592555997 35.0 31.0 35.0 2.0 35.0 120-121 29.35380960706535 35.0 31.0 35.0 2.0 35.0 122-123 29.234666587535443 35.0 31.0 35.0 2.0 35.0 124-125 28.32253325783787 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28081.0 3 10890.0 4 6176.0 5 5278.0 6 5658.0 7 6856.0 8 7701.0 9 8553.0 10 9984.0 11 11186.0 12 11938.0 13 14155.0 14 18333.0 15 15496.0 16 8242.0 17 6897.0 18 6119.0 19 5512.0 20 5181.0 21 5506.0 22 6599.0 23 8563.0 24 12302.0 25 16858.0 26 21192.0 27 24101.0 28 27383.0 29 33550.0 30 38837.0 31 46302.0 32 57523.0 33 74023.0 34 103008.0 35 166028.0 36 330945.0 37 535097.0 38 169917.0 39 341.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.80788986395734 17.671252456481927 12.64479927109871 24.876058408462022 2 16.994762303987407 19.80459693908104 37.36510098979409 25.835539767137465 3 18.927735462304053 24.46221795353777 29.30384732417836 27.30619925997982 4 13.374851942165694 15.632981347120323 35.40147078806892 35.59069592264507 5 14.221590912129587 37.24846015660222 33.31056837696201 15.219380554306186 6 33.383317272160404 35.08770897796393 16.68668519851503 14.842288551360635 7 29.28339613984596 31.797795649158385 20.470752057855787 18.448056153139873 8 28.070351796411185 33.122536026876254 19.207721522980684 19.59939065373187 9 28.01904366740312 14.050586345622031 18.828934226201298 39.10143576077355 10-11 26.41180234005277 24.714471373347312 26.737307246955694 22.136419039644224 12-13 26.8507787454895 22.571494950191674 27.07957541882116 23.498150885497665 14-15 24.36849699276644 23.404850873457185 24.804724312194075 27.421927821582305 16-17 23.999726219752496 26.436316949831372 25.262136566079164 24.301820264336968 18-19 24.0319677296287 25.167996873059195 27.553331905677393 23.24670349163471 20-21 25.1464114750662 24.110729426460587 27.38768881625948 23.355170282213734 22-23 24.952543659832955 23.765948003892756 27.20884532708781 24.072663009186478 24-25 24.354478750263695 24.325682859653856 27.262195273570317 24.057643116512132 26-27 24.04426094031104 24.35308866491831 27.437088148378475 24.165562246392174 28-29 24.141346947222296 24.43184704588271 27.390349247159566 24.03645675973543 30-31 24.422449480540106 24.345334005731786 27.220425653890562 24.011790859837546 32-33 24.07741867865056 24.411845467444856 27.17514440002235 24.33559145388223 34-35 24.299670922542592 24.487138384464185 27.27297278579964 23.940217907193578 36-37 24.477137132287474 24.31577703376557 26.996974932635165 24.21011090131179 38-39 24.494271701515622 24.557937288460995 26.86243901499898 24.085351995024403 40-41 24.449287265750577 24.46321683825975 26.932414035020173 24.1550818609695 42-43 24.449452982129678 24.48375522688141 26.70479215459805 24.36199963639086 44-45 24.406226771266155 24.554008310204868 26.54500826207627 24.494756656452708 46-47 24.64528245585984 24.66891870573885 26.22474009483377 24.46105874356754 48-49 24.843014842330323 24.88245499010119 25.669252514275996 24.60527765329249 50-51 24.687939581734096 25.153038947760475 25.587764477638196 24.57125699286723 52-53 24.80527873838217 25.125801071866043 25.620288392556567 24.448631797195223 54-55 24.71508621277431 25.01941849592686 25.65169274471516 24.613802546583667 56-57 24.60664260869937 25.088933905476573 25.520100239153592 24.78432324667046 58-59 24.622219655380047 24.936871725270883 25.717218484258304 24.723690135090763 60-61 24.72093497712104 25.079581026118618 25.751417351110284 24.44806664565006 62-63 24.60227161819573 25.055798423499002 25.795570406018932 24.546359552286336 64-65 24.630245645501596 24.73209102822125 25.902016132158888 24.73564719411826 66-67 24.526136709088192 25.000608262585487 25.763757228632674 24.70949779969365 68-69 24.688308041981415 25.044722240791494 25.577886222341068 24.68908349488602 70-71 24.511666110526452 24.82738263302446 25.906856139360183 24.754095117088905 72-73 24.902459058473227 25.06069570648733 25.493881231069356 24.542964003970088 74-75 24.630881437933347 25.276857304281396 25.406913457843917 24.685347799941333 76-77 24.61258502755667 24.968537030050715 25.485099239033282 24.933778703359337 78-79 24.512914559789323 25.10250595059979 25.533280973938233 24.851298515672653 80-81 24.557528217093605 25.216138752348694 25.347187034322026 24.87914599623567 82-83 24.683565286533096 25.15633914807129 25.31365416256491 24.846441402830706 84-85 24.47000675375427 25.196583445227482 25.274869242932485 25.058540558085763 86-87 24.34832246257174 25.23579905110884 25.41250295776512 25.0033755285543 88-89 24.44638807609612 25.05135902545798 25.377521926687415 25.124730971758485 90-91 24.389246808137457 24.969493087951882 25.49891233901655 25.14234776489411 92-93 24.39747818435904 25.229171506937508 25.396483349958466 24.976866958744985 94-95 24.37166791566219 24.94108154367826 25.50458661634681 25.18266392431274 96-97 24.350185668875802 24.985790783659002 25.414419881674522 25.249603665790676 98-99 24.291127920856663 25.00762587370056 25.312660821722982 25.388585383719796 100-101 24.263972392765158 25.072997843151356 25.460599613013173 25.20243015107031 102-103 24.32492259718428 24.865011253852096 25.585824557538743 25.224241591424885 104-105 24.201913642792025 25.02613280007272 25.41706879543164 25.354884761703616 106-107 24.209913693082722 24.992434376908115 25.41945097636638 25.378200953642782 108-109 24.2533547064824 24.91738404420445 25.370204243702027 25.45905700561112 110-111 24.26956217440041 24.99752045060163 25.393419610067795 25.339497764930165 112-113 24.151852220215236 25.01179059896243 25.47146354218468 25.36489363863766 114-115 23.94848766528692 25.18405760022414 25.43827216437408 25.429182570114854 116-117 23.97334675816298 25.156289560314548 25.35648563911288 25.513878042409594 118-119 24.000104805352134 24.819832380950345 25.490693578827393 25.689369234870128 120-121 23.7542581638311 24.95854790130203 25.584807438225543 25.70238649664132 122-123 23.800405069138712 25.061246924286934 25.481393874131285 25.656954132443065 124-125 23.747889506119026 25.053226963193843 25.480352953193965 25.718530577493166 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 38.0 1 41.0 2 76.5 3 111.0 4 92.5 5 86.5 6 149.5 7 328.5 8 532.0 9 626.0 10 563.5 11 510.5 12 584.0 13 718.0 14 846.5 15 977.5 16 1197.5 17 1438.5 18 1642.5 19 1839.5 20 2124.5 21 2453.5 22 2783.5 23 3171.0 24 3527.0 25 4241.0 26 5566.5 27 6972.0 28 7753.0 29 9066.5 30 11446.5 31 13563.5 32 16129.5 33 18888.0 34 21789.5 35 24953.0 36 28356.0 37 31590.0 38 33670.0 39 35847.0 40 38236.5 41 40734.5 42 43754.5 43 48889.0 44 54574.5 45 58749.5 46 62674.0 47 66419.5 48 71075.0 49 75614.0 50 75944.5 51 75177.5 52 75124.5 53 75455.5 54 77484.0 55 77465.5 56 73507.5 57 67923.0 58 63933.0 59 59682.0 60 53082.5 61 46833.0 62 40700.5 63 32168.0 64 24470.0 65 19415.5 66 15169.0 67 12000.5 68 9779.0 69 7930.0 70 6653.5 71 5389.5 72 4203.5 73 3830.0 74 3326.5 75 2574.0 76 1763.5 77 1044.0 78 610.5 79 327.0 80 173.5 81 74.0 82 44.5 83 22.0 84 10.0 85 8.5 86 6.5 87 4.5 88 3.5 89 1.0 90 0.0 91 1.0 92 1.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12265339828509804 2 0.001229742005473956 3 0.01935506982528574 4 0.05929495148133118 5 0.02555724689637178 6 0.020157075481029626 7 0.015077706327985024 8 0.005881374808788485 9 0.004437764628449493 10-11 0.007458652598418124 12-13 0.004892234500037694 14-15 0.003769426581996256 16-17 0.010586474655819272 18-19 0.006015042418079132 20-21 0.01277862344818589 22-23 0.009142864475480282 24-25 0.013500428538355385 26-27 0.009329999128487186 28-29 0.013901431366227329 30-31 0.020745212961908472 32-33 0.013126159232341575 34-35 0.020665012396334084 36-37 0.014436101803389919 38-39 0.020531344787043437 40-41 0.010105271262372942 42-43 0.009142864475480282 44-45 0.017162921032919123 46-47 0.016147047202310204 48-49 0.020798680005624733 50-51 0.014997505762410636 52-53 0.021092748746064156 54-55 0.0168688522924797 56-57 0.02047787774332718 58-59 0.016521316508324016 60-61 0.019328336303427614 62-63 0.021948221445524302 64-65 0.017082720467344735 66-67 0.012324153576597689 68-69 0.023311631060288905 70-71 0.014917305196836247 72-73 0.017483723295216677 74-75 0.018686731778832504 76-77 0.013660829669504163 78-79 0.028952404172354226 80-81 0.02082541352748286 82-83 0.009517133781494094 84-85 0.012832090491902148 86-87 0.012484554707746466 88-89 0.020665012396334084 90-91 0.012992491623050925 92-93 0.008046790079296973 94-95 0.007565586685850643 96-97 0.002459484010947912 98-99 0.001791145964494675 100-101 0.011842950183151358 102-103 0.0033416902322661846 104-105 0.0029941544481105016 106-107 4.812033934463306E-4 108-109 0.01943527039086013 110-111 6.14871002736978E-4 112-113 0.009463666737777834 114-115 0.002165415270508488 116-117 0.007725987816999419 118-119 0.009490400259635965 120-121 0.004277363497300716 122-123 6.950715683113664E-4 124-125 0.026091917333534373 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1870311.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.641157317185275 #Duplication Level Percentage of deduplicated Percentage of total 1 77.2140340745969 40.646361148153595 2 11.467015989702666 12.072739853452342 3 3.9579271826395352 6.250496024138751 4 2.022607179722006 4.258895309544582 5 1.2184949987814964 3.2071493460530114 6 0.7895722519761776 2.4938398277737326 7 0.5793503192735137 2.1348369909046983 8 0.41872014193846224 1.763353029092539 9 0.3210923527509726 1.5212405749058187 >10 1.8694370620692151 17.803061145131764 >50 0.10130147652685415 3.6005220105300975 >100 0.039428396419070845 3.5472419940322824 >500 6.111441619045946E-4 0.19345696696806533 >1k 4.0742944126972975E-4 0.5068057793187787 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3575 0.19114468128562576 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3171 0.16954399562425715 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.0693408743251791E-4 7 0.0 0.0 0.0 0.0 1.0693408743251791E-4 8 0.0 0.0 0.0 0.0 1.0693408743251791E-4 9 0.0 0.0 0.0 5.3467043716258954E-5 2.1386817486503581E-4 10-11 0.0 0.0 0.0 5.3467043716258954E-5 2.1386817486503581E-4 12-13 0.0 0.0 0.0 5.3467043716258954E-5 2.4060169672316528E-4 14-15 0.0 2.6733521858129477E-5 0.0 5.3467043716258954E-5 2.673352185812948E-4 16-17 0.0 5.3467043716258954E-5 0.0 5.3467043716258954E-5 3.208022622975537E-4 18-19 0.0 5.3467043716258954E-5 0.0 5.3467043716258954E-5 3.742693060138127E-4 20-21 0.0 5.3467043716258954E-5 0.0 5.3467043716258954E-5 4.2773634973007163E-4 22-23 0.0 5.3467043716258954E-5 0.0 1.336676092906474E-4 5.61403959020719E-4 24-25 0.0 5.3467043716258954E-5 0.0 2.1386817486503581E-4 6.416045245951074E-4 26-27 0.0 5.3467043716258954E-5 0.0 4.8120339344633056E-4 6.950715683113664E-4 28-29 0.0 5.3467043716258954E-5 0.0 0.0015772777896296392 6.950715683113664E-4 30-31 0.0 5.3467043716258954E-5 0.0 0.005213036762335248 7.485386120276254E-4 32-33 0.0 5.3467043716258954E-5 0.0 0.012056818358016395 8.020056557438843E-4 34-35 0.0 5.3467043716258954E-5 0.0 0.02144028453021984 8.554726994601433E-4 36-37 0.0 8.020056557438843E-5 0.0 0.03584965281175163 8.554726994601433E-4 38-39 0.0 1.0693408743251791E-4 0.0 0.05884048160974298 8.822062213182727E-4 40-41 0.0 1.0693408743251791E-4 0.0 0.08752555056351591 9.089397431764022E-4 42-43 0.0 1.0693408743251791E-4 0.0 0.11511454512110553 9.089397431764022E-4 44-45 0.0 1.0693408743251791E-4 0.0 0.14569769412680567 9.089397431764022E-4 46-47 0.0 1.0693408743251791E-4 0.0 0.1802106708456508 9.356732650345317E-4 48-49 0.0 1.0693408743251791E-4 0.0 0.21868020879949912 0.001069340874325179 50-51 0.0 1.0693408743251791E-4 0.0 0.2596894313298697 0.001069340874325179 52-53 0.0 1.0693408743251791E-4 0.0 0.30168779416899116 0.001176274961757697 54-55 0.0 1.0693408743251791E-4 0.0 0.3477496523305482 0.0012832090491902148 56-57 0.0 1.0693408743251791E-4 0.0 0.3995324841697451 0.0012832090491902148 58-59 0.0 1.0693408743251791E-4 0.0 0.45783829534232545 0.0012832090491902148 60-61 0.0 1.0693408743251791E-4 0.0 0.5158767712963246 0.0012832090491902148 62-63 0.0 1.0693408743251791E-4 0.0 0.5771232698732991 0.0014436101803389917 64-65 0.0 1.0693408743251791E-4 0.0 0.645641286395685 0.0014436101803389917 66-67 0.0 1.0693408743251791E-4 0.0 0.7200139442050012 0.0014703437021971212 68-69 0.0 1.0693408743251791E-4 0.0 0.8086623026865585 0.0014970772240552508 70-71 0.0 1.0693408743251791E-4 0.0 0.9095278806572811 0.0014970772240552508 72-73 0.0 1.0693408743251791E-4 0.0 1.0136816818165535 0.0018713465300690634 74-75 0.0 1.0693408743251791E-4 0.0 1.118690955675286 0.0018713465300690634 76-77 0.0 1.0693408743251791E-4 0.0 1.237173924550516 0.0018713465300690634 78-79 0.0 1.0693408743251791E-4 0.0 1.371376204278326 0.0018713465300690634 80-81 0.0 1.0693408743251791E-4 0.0 1.523115674345069 0.0019782806175015813 82-83 0.0 1.0693408743251791E-4 0.0 1.6835702725375619 0.0021921487923666173 84-85 0.0 1.0693408743251791E-4 0.0 1.85124292163175 0.0021921487923666173 86-87 0.0 1.336676092906474E-4 0.0 2.0252246818844566 0.0021921487923666173 88-89 0.0 1.6040113114877685E-4 0.0 2.225939963995293 0.0022188823142247466 90-91 0.0 1.6040113114877685E-4 0.0 2.4556878508440576 0.002245615836082876 92-93 0.0 1.6040113114877685E-4 0.0 2.6966905503950946 0.002299082879799135 94-95 0.0 1.6040113114877685E-4 0.0 2.9433340230581972 0.002352549923515394 96-97 0.0 1.6040113114877685E-4 0.0 3.203370990172223 0.002352549923515394 98-99 0.0 1.6040113114877685E-4 0.0 3.491692023412149 0.002352549923515394 100-101 0.0 1.6040113114877685E-4 0.0 3.8142854316741976 0.002352549923515394 102-103 0.0 1.6040113114877685E-4 0.0 4.159441932384507 0.0024862175328060412 104-105 0.0 1.6040113114877685E-4 0.0 4.520932614950134 0.0025664180983804297 106-107 0.0 1.6040113114877685E-4 0.0 4.887663067799954 0.0025931516202385594 108-109 0.0 1.6040113114877685E-4 0.0 5.281634979423208 0.002646618663954818 110-111 0.0 1.6040113114877685E-4 0.0 5.711616944989363 0.0027535527513873363 112-113 0.0 1.8713465300690635E-4 0.0 6.183196270566766 0.0028337533169617247 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3010 0.0 85.56612 1 GTATCAA 6275 0.0 60.99664 1 TCAACGC 8290 0.0 46.03326 4 CAACGCA 8475 0.0 44.93772 5 ATCAACG 8680 0.0 43.966125 3 AACGCAG 8760 0.0 43.738068 6 TATCAAC 9050 0.0 42.302444 2 GTACATG 9785 0.0 37.715023 1 ACGCAGA 10290 0.0 37.060295 7 TACATGG 10255 0.0 35.415817 2 CGCAGAG 10880 0.0 35.05059 8 ACATGGG 10310 0.0 34.705288 3 GCAGAGT 11465 0.0 32.84701 9 CATGGGG 8675 0.0 31.156927 4 ATGGGGG 4615 0.0 29.01226 5 CAGAGTA 11165 0.0 28.347786 10-11 AGAGTAC 10715 0.0 27.73381 10-11 GAGTACT 6385 0.0 26.741589 12-13 GTGGTAT 1145 0.0 25.513802 1 TATACCG 210 9.806536E-8 25.503159 5 >>END_MODULE