FastQCFastQC Report
Mon 15 Aug 2016
SRR2921484_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921484_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909087
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19650.074.514781
GTATCAA41250.058.5830421
TCAACGC52750.045.006194
CAACGCA53250.044.3601235
AACGCAG55600.042.37046
ATCAACG56700.041.973583
TATCAAC60750.039.860912
GTACATG101050.037.756491
TACATGG104250.036.240712
ACATGGG106000.035.586273
ACGCAGA67500.034.8125237
CATGGGG88550.032.58934
CGCAGAG72800.032.113828
GTATAGG5150.030.0494121
ATGGGGG45950.029.7827075
GCAGAGT79650.028.9777979
AGAGTAC70150.025.6507510-11
CAGAGTA74500.025.15108110-11
ATGGGTA12200.023.897815
GTGGTAT10350.023.5783481
Produced by FastQC (version 0.11.5)