##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921484_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1909087
Sequences flagged as poor quality	0
Sequence length	125
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.001923432509887	31.0	30.0	34.0	26.0	34.0
2	30.094460336275926	31.0	30.0	34.0	26.0	34.0
3	30.180918941881643	31.0	31.0	34.0	26.0	34.0
4	33.57573017887608	37.0	35.0	37.0	28.0	37.0
5	33.59587436298084	37.0	35.0	37.0	28.0	37.0
6	33.66809422514532	37.0	35.0	37.0	28.0	37.0
7	33.559193478348554	37.0	35.0	37.0	27.0	37.0
8	33.53159232659381	37.0	35.0	37.0	27.0	37.0
9	35.034371403712875	39.0	35.0	39.0	27.0	39.0
10-11	34.9191734059265	39.0	35.0	39.0	27.0	39.0
12-13	34.76844219252449	39.0	35.0	39.0	27.0	39.0
14-15	35.95025161241997	40.0	36.0	41.0	27.0	41.0
16-17	35.8763827944981	40.0	36.0	41.0	27.0	41.0
18-19	35.89070377620297	40.0	36.0	41.0	27.0	41.0
20-21	35.90812074043771	40.0	36.0	41.0	27.0	41.0
22-23	35.88352678531675	40.0	36.0	41.0	26.5	41.0
24-25	35.84399218055542	40.0	36.0	41.0	26.5	41.0
26-27	35.76712899935938	40.0	36.0	41.0	26.0	41.0
28-29	35.7236865056438	40.0	35.5	41.0	26.0	41.0
30-31	35.629079502400884	40.0	35.0	41.0	26.0	41.0
32-33	35.52488336047545	40.0	35.0	41.0	25.5	41.0
34-35	35.35436886846959	40.0	35.0	41.0	25.0	41.0
36-37	35.21577984659683	40.0	35.0	41.0	25.0	41.0
38-39	35.088486538329576	40.0	35.0	41.0	24.0	41.0
40-41	34.93298210086811	39.0	35.0	41.0	24.0	41.0
42-43	34.74205444801625	39.0	34.0	41.0	23.0	41.0
44-45	34.547761573987984	39.0	34.0	41.0	22.5	41.0
46-47	34.29579270090886	39.0	33.5	41.0	21.0	41.0
48-49	34.14167348056951	39.0	33.0	41.0	20.0	41.0
50-51	33.93695153756744	38.0	33.0	40.5	19.0	41.0
52-53	33.74262330632391	38.0	33.0	40.0	18.0	41.0
54-55	33.53054575302225	38.0	33.0	40.0	17.5	41.0
56-57	33.28356303301002	37.0	33.0	40.0	16.0	41.0
58-59	33.03708945689746	37.0	33.0	40.0	14.5	41.0
60-61	32.72497429399498	36.0	32.0	40.0	13.0	41.0
62-63	32.40545794927104	36.0	32.0	39.0	10.5	41.0
64-65	32.089972588991486	35.0	32.0	39.0	8.0	41.0
66-67	32.03695614710068	35.0	32.0	39.0	8.0	41.0
68-69	31.78967511695381	35.0	32.0	38.5	7.0	40.0
70-71	31.48973435993226	35.0	32.0	37.0	6.5	40.0
72-73	31.16376754961927	35.0	31.0	37.0	2.0	39.0
74-75	30.86406355498728	35.0	31.0	36.0	2.0	39.0
76-77	30.59343707227591	35.0	31.0	36.0	2.0	38.5
78-79	30.32726009867544	35.0	31.0	35.5	2.0	37.0
80-81	30.089946398461674	35.0	31.0	35.0	2.0	37.0
82-83	29.906914928444856	35.0	31.0	35.0	2.0	36.5
84-85	29.718359875689266	35.0	30.5	35.0	2.0	36.0
86-87	29.572850530122516	35.0	30.0	35.0	2.0	36.0
88-89	29.451678734389787	35.0	30.0	35.0	2.0	35.5
90-91	29.347785878799655	35.0	30.0	35.0	2.0	35.0
92-93	29.253785972037942	34.0	30.0	35.0	2.0	35.0
94-95	29.195188590148064	34.0	30.0	35.0	2.0	35.0
96-97	29.09656631677865	34.0	30.0	35.0	2.0	35.0
98-99	29.035163405334593	34.0	30.0	35.0	2.0	35.0
100-101	28.971138298045084	34.0	30.0	35.0	2.0	35.0
102-103	27.993060819124537	33.5	28.0	34.5	2.0	35.0
104-105	28.81903417706998	34.0	29.5	35.0	2.0	35.0
106-107	29.144537415005182	35.0	30.0	35.0	2.0	35.0
108-109	29.184188043813613	35.0	31.0	35.0	2.0	35.0
110-111	29.120839437909325	35.0	31.0	35.0	2.0	35.0
112-113	29.04968919698264	35.0	30.0	35.0	2.0	35.0
114-115	28.92597325318333	35.0	30.0	35.0	2.0	35.0
116-117	28.861420668623275	35.0	30.0	35.0	2.0	35.0
118-119	28.70604299332613	35.0	30.0	35.0	2.0	35.0
120-121	28.53048001479241	35.0	30.0	35.0	2.0	35.0
122-123	28.395983525109123	35.0	30.0	35.0	2.0	35.0
124-125	27.54787209802382	34.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	64211.0
3	11456.0
4	7753.0
5	6631.0
6	7294.0
7	8641.0
8	9398.0
9	9623.0
10	10193.0
11	10879.0
12	10962.0
13	11590.0
14	12821.0
15	11215.0
16	7850.0
17	7147.0
18	6391.0
19	5982.0
20	5902.0
21	6180.0
22	7431.0
23	10082.0
24	14548.0
25	19700.0
26	23525.0
27	25366.0
28	30240.0
29	35569.0
30	40087.0
31	47561.0
32	61332.0
33	78273.0
34	112190.0
35	180230.0
36	345309.0
37	504616.0
38	140754.0
39	155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.31932283462917	16.70544207634983	11.958231547882916	25.017003541138088
2	17.14966682838859	19.031439370520992	37.75338908722819	26.065504713862225
3	19.525407282516323	23.593831373983168	28.516849293680373	28.363912049820133
4	13.642401108703798	14.77714582127129	34.94837663443426	36.63207643559065
5	14.778115275366769	36.78398273819473	33.09976311127482	15.338138875163676
6	34.236459268412446	33.84678510958407	17.083512161072306	14.833243460931184
7	30.403050942310657	30.76738870484816	20.257714077910535	18.57184627493065
8	28.359371888839753	32.86600868138156	19.237214244843464	19.537405184935224
9	27.94917379383121	14.367294842191136	18.538487212496655	39.145044151481
10-11	26.84520960350777	24.430813825995305	26.507002939822584	22.216973630674342
12-13	26.95114132987783	22.08901081425353	26.682992276206885	24.276855579661753
14-15	24.348162573118106	23.307826869736786	23.938750952660783	28.40525960448432
16-17	24.643470492144733	26.308554271633923	24.477522783750537	24.57045245247081
18-19	24.734330504486433	25.444450733100027	25.95280992852943	23.868408833884114
20-21	25.849439699202254	24.42291454947686	25.624412668180646	24.10323308314024
22-23	25.631975934459135	24.006503775307927	25.493331796045588	24.86818849418735
24-25	25.049281390581683	24.5633598036816	25.449507605398757	24.93785120033796
26-27	24.68553652754767	24.57700552428571	25.686002671088957	25.05145527707766
28-29	24.7935824589307	24.658665265700925	25.63683456856762	24.910917706800753
30-31	25.04563840443893	24.585520487431523	25.511835328094733	24.857005780034815
32-33	24.69858727977919	24.618689226445863	25.519291488485795	25.16343200528915
34-35	25.057168847746414	24.667047792318307	25.565845831704554	24.70993752823072
36-37	25.088796437818388	24.47197072147908	25.470237897153613	24.968994943548918
38-39	25.05102905871991	24.68061142102269	25.422494924610117	24.845864595647285
40-41	25.073177352660732	24.53298424462971	25.55679901520092	24.837039387508636
42-43	24.966438063782224	24.59833771785123	25.46881235949256	24.96641185887399
44-45	24.890705535306378	24.624410420466937	25.399003738666014	25.08588030556067
46-47	24.94622421671326	24.710601376785842	25.339610319499435	25.00356408700146
48-49	25.16811013110009	24.82385873377429	24.96561547449073	25.042415660634887
50-51	25.03742449266328	25.070204496389355	24.980721112916502	24.91164989803087
52-53	25.167454234417058	24.888783134449767	25.072720735603653	24.871041895529522
54-55	25.020430975008836	24.851611774006034	25.20235115389483	24.9256060970903
56-57	24.927447525879405	24.896791550898875	25.038673691513903	25.137087231707817
58-59	25.082699532847535	24.738643274369604	25.04064559218368	25.138011600599185
60-61	25.04027433443535	24.861414109234424	25.231107479354577	24.867204076975657
62-63	24.868509378188357	25.039865095364434	25.125673959852456	24.96595156659475
64-65	24.932637719313874	24.70841119340494	25.228025643256068	25.130925444025117
66-67	24.863370798291808	24.907228379051062	25.189604129005218	25.039796693651915
68-69	24.977620122372148	24.96352544966067	25.04057458059834	25.01827984736884
70-71	24.87957825991601	24.776732080330596	25.22427672932903	25.11941293042436
72-73	25.089048058181206	24.999030662502882	25.032616896862493	24.87930438245342
74-75	24.801191801179225	25.190843294703548	25.00206982705678	25.005895077060448
76-77	24.921495980353168	24.83231604280008	25.044210089489084	25.201977887357668
78-79	24.91129131136907	24.942624917867175	25.09140715139791	25.054676619365846
80-81	24.867837970661864	25.14560090367916	24.874439522969734	25.112121602689243
82-83	25.043739209270537	25.047380323262814	24.917269436934465	24.991611030532184
84-85	24.80565815324165	25.09569089718402	24.92950884086444	25.16914210870989
86-87	24.660750827494507	25.172990621205027	25.013909451826144	25.152349099474325
88-89	24.81639047085143	24.993398544536248	24.963141873660717	25.227069110951604
90-91	24.78407190076973	24.945111016930063	25.01874162127728	25.252075461022926
92-93	24.801942655512637	25.195581873263734	24.95206885215412	25.05040661906951
94-95	24.805167794587447	24.94373493180611	25.015480752003068	25.235616521603376
96-97	24.784429017163955	24.99135620603452	24.98093126664585	25.243283510155674
98-99	24.740465849198046	24.932723069452003	24.950351013488913	25.376460067861036
100-101	24.64635648628239	25.130567503379602	25.023855630413355	25.199220379924654
102-103	24.812771531372114	24.833961029657704	25.169902321389426	25.183365117580763
104-105	24.588414634146343	25.045757899589304	25.068833291425697	25.296994174838655
106-107	24.617210862811444	25.12446196572768	25.019219017699307	25.23910815376157
108-109	24.69110223022474	25.018473227375633	24.98617618468984	25.304248357709785
110-111	24.696805042951365	25.18078546895786	25.02064604236387	25.101763445726906
112-113	24.70588882407653	25.138225377102717	25.031228235511055	25.1246575633097
114-115	24.478512807765778	25.36455450788252	25.09744284719343	25.059489837158278
116-117	24.566548250752575	25.380477587280335	24.94819794866234	25.104776213304753
118-119	24.693808753932245	25.084079525447546	24.951967684057365	25.270144036562847
120-121	24.399750611249516	25.17241415565033	25.174543212225934	25.25329202087422
122-123	24.47818463190177	25.295840122022177	25.02748878638285	25.198486459693196
124-125	24.483096221027537	25.373531111135556	25.0313905996351	25.111982068201804
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	89.0
1	107.0
2	143.0
3	163.5
4	159.0
5	156.5
6	161.0
7	174.0
8	229.5
9	300.5
10	342.0
11	373.0
12	398.5
13	462.5
14	554.5
15	651.5
16	741.5
17	811.0
18	870.5
19	965.0
20	1138.0
21	1312.5
22	1519.5
23	1846.0
24	2229.0
25	2797.0
26	3754.5
27	4865.5
28	5779.5
29	7146.5
30	9247.0
31	11212.0
32	13412.5
33	15767.5
34	18178.0
35	21054.0
36	24541.0
37	28407.0
38	31620.0
39	34940.5
40	37908.0
41	40531.5
42	43983.0
43	49707.0
44	55496.0
45	59469.0
46	64113.0
47	68544.0
48	73640.0
49	78710.5
50	79555.5
51	79766.5
52	80478.5
53	81165.0
54	82033.0
55	81098.5
56	77394.0
57	71807.0
58	68270.5
59	63948.5
60	57004.5
61	50544.5
62	44135.0
63	35325.0
64	27106.5
65	21729.0
66	17004.5
67	13175.0
68	10803.5
69	9081.0
70	7841.5
71	6439.5
72	5198.5
73	4688.5
74	3925.0
75	3095.0
76	2236.0
77	1361.5
78	800.5
79	510.0
80	317.0
81	156.0
82	88.5
83	48.5
84	32.5
85	26.5
86	23.5
87	20.5
88	16.5
89	13.5
90	10.5
91	10.0
92	7.0
93	6.0
94	10.5
95	11.0
96	12.5
97	11.0
98	6.0
99	6.0
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034623880420326575
2	0.02414766849284501
3	0.02414766849284501
4	0.028914344919849122
5	0.03121911154389507
6	0.04326675526049886
7	0.036300074328723626
8	0.03326197286975397
9	0.028495296442749857
10-11	0.0334453065784849
12-13	0.03318340128029786
14-15	0.03229292326646192
16-17	0.03428340353268343
18-19	0.046566762017655555
20-21	0.05730487924332417
22-23	0.05052153202027985
24-25	0.061076315537217526
26-27	0.04591199877218796
28-29	0.046069141951100186
30-31	0.04612152301073759
32-33	0.05397868195634877
34-35	0.03692864704437252
36-37	0.04874057599260798
38-39	0.05790726142915435
40-41	0.04612152301073759
42-43	0.054869159970184704
44-45	0.055183446328009145
46-47	0.061023934477580116
48-49	0.04654057148783686
50-51	0.04774533585949724
52-53	0.05777630878006084
54-55	0.043607232148142015
56-57	0.042585801485212564
58-59	0.04313580261140535
60-61	0.032135780087549706
62-63	0.040647702278628475
64-65	0.03923341366841846
66-67	0.033366734989028785
68-69	0.02956910816531672
70-71	0.04701200102457353
72-73	0.02970006081441024
74-75	0.037452457640746596
76-77	0.03069530094752099
78-79	0.03106196836498284
80-81	0.023309571538646483
82-83	0.017311940210163287
84-85	0.01765241709780644
86-87	0.01639527166650865
88-89	0.021842901868799065
90-91	0.012257167955153431
92-93	0.018830990939648115
94-95	0.013880980803913074
96-97	0.010633355106393788
98-99	0.009742877092557856
100-101	0.010921450934399533
102-103	0.006416679805582458
104-105	0.006652394573950795
106-107	0.008930970668178035
108-109	0.012754788021708806
110-111	0.006573822984494683
112-113	0.008250016892891733
114-115	0.007726206296517655
116-117	0.007202395700143576
118-119	0.24304811671757234
120-121	0.35807692368131994
122-123	0.030459586179152655
124-125	0.003980960532442995
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1909087.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.25408763605181
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.78493606696617	44.31974697911854
2	10.801413472572383	12.152473201586353
3	3.7485787833728175	6.326186381714968
4	1.9049008699244252	4.286338418988798
5	1.1139220705964599	3.133133488953278
6	0.7448725169968026	2.5141274309294777
7	0.5126924691663474	2.018873296357925
8	0.3899542044601201	1.754921439339717
9	0.287708388634913	1.4566295617106084
>10	1.6047124727903803	15.920433443292954
>50	0.07842441878972455	2.991443160508657
>100	0.026664165751508186	2.586428480887069
>500	9.385384445497781E-4	0.3243824573000108
>1k	2.815615333649333E-4	0.2148822593116933
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.238105963740783E-5
2	0.0	0.0	0.0	0.0	5.238105963740783E-5
3	0.0	0.0	0.0	0.0	5.238105963740783E-5
4	0.0	0.0	0.0	5.238105963740783E-5	5.238105963740783E-5
5	0.0	0.0	0.0	5.238105963740783E-5	5.238105963740783E-5
6	0.0	0.0	0.0	5.238105963740783E-5	1.0476211927481566E-4
7	0.0	0.0	0.0	5.238105963740783E-5	1.0476211927481566E-4
8	0.0	0.0	0.0	5.238105963740783E-5	1.571431789122235E-4
9	0.0	0.0	0.0	5.238105963740783E-5	1.571431789122235E-4
10-11	0.0	0.0	0.0	5.238105963740783E-5	1.571431789122235E-4
12-13	0.0	0.0	0.0	5.238105963740783E-5	2.0952423854963132E-4
14-15	0.0	0.0	0.0	5.238105963740783E-5	2.8809582800574306E-4
16-17	0.0	0.0	0.0	7.857158945611175E-5	3.4047688764315086E-4
18-19	0.0	0.0	0.0	1.0476211927481566E-4	3.666674174618548E-4
20-21	0.0	0.0	0.0	1.309526490935196E-4	4.452390069179666E-4
22-23	0.0	0.0	0.0	1.833337087309274E-4	6.547632454675979E-4
24-25	0.0	0.0	0.0	3.14286357824447E-4	7.857158945611174E-4
26-27	0.0	0.0	0.0	3.4047688764315086E-4	8.642874840172291E-4
28-29	0.0	0.0	0.0	9.952401331107487E-4	8.90478013835933E-4
30-31	0.0	0.0	0.0	0.004321437420086146	8.90478013835933E-4
32-33	0.0	0.0	0.0	0.01063335510639379	9.952401331107487E-4
34-35	0.0	0.0	0.0	0.018883371999285522	0.0010476211927481565
36-37	0.0	0.0	0.0	0.032214351677005815	0.0010476211927481565
38-39	0.0	0.0	0.0	0.05316677553196894	0.0011261927822042682
40-41	0.0	0.0	0.0	0.0761882512426097	0.00120476437166038
42-43	0.0	0.0	0.0	0.09949782278125617	0.00120476437166038
44-45	0.0	0.0	0.0	0.125871686308691	0.001257145431297788
46-47	0.0	0.0	0.0	0.15546698500382644	0.0013619075505726034
48-49	0.0	0.0	0.0	0.18906943476122356	0.001519050729484827
50-51	0.0	0.0	0.0	0.22513379432157885	0.001519050729484827
52-53	0.0	0.0	0.0	0.26017672321900465	0.0016761939083970506
54-55	0.0	0.0	0.0	0.29757679980011387	0.001780956027671866
56-57	0.0	0.0	0.0	0.3399792675765955	0.001833337087309274
58-59	0.0	0.0	0.0	0.38937460681467106	0.0018857181469466818
60-61	0.0	0.0	0.0	0.44309138347283283	0.0018857181469466818
62-63	0.0	0.0	0.0	0.4986414972183038	0.0018857181469466818
64-65	0.0	0.0	0.0	0.5577011419594812	0.0019380992065840897
66-67	0.0	0.0	0.0	0.6245393740568135	0.0019380992065840897
68-69	0.0	0.0	0.0	0.7047347763616849	0.0019380992065840897
70-71	0.0	0.0	0.0	0.7912159058230452	0.0019380992065840897
72-73	0.0	0.0	0.0	0.8805256125048256	0.0023833382135020563
74-75	0.0	0.0	0.0	0.9721924668702893	0.002514290862595576
76-77	0.0	0.0	0.0	1.0742569615737785	0.0025404813924142797
78-79	0.0	0.0	0.0	1.1916429162212094	0.0025666719222329835
80-81	0.0	0.0	0.0	1.3207622282274198	0.0026452435116890954
82-83	0.0	0.0	0.0	1.4599125131541937	0.0027238151011452073
84-85	0.0	0.0	0.0	1.5990627980809675	0.0027238151011452073
86-87	0.0	0.0	0.0	1.7532988281833148	0.0028023866906013188
88-89	0.0	0.0	0.0	1.9270206124707778	0.0028809582800574307
90-91	0.0	0.0	0.0	2.1227948228655897	0.0028809582800574307
92-93	0.0	0.0	0.0	2.3326071572432268	0.0028809582800574307
94-95	0.0	0.0	0.0	2.5448552108940032	0.003038101458969654
96-97	0.0	0.0	0.0	2.772110438130897	0.0031428635782444697
98-99	0.0	0.0	0.0	3.022099045250426	0.0032738162273379893
100-101	0.0	0.0	0.0	3.302756762787657	0.003300006757156693
102-103	0.0	0.0	0.0	3.6117526335887256	0.003430959406250213
104-105	0.0	0.0	0.0	3.933189006053679	0.003457149936068917
106-107	0.0	0.0	0.0	4.262482537464244	0.0034833404658876207
108-109	0.0	0.0	0.0	4.603745141001955	0.003561912055343732
110-111	0.0	0.0	0.0	4.989453073642008	0.0037976268237120674
112-113	0.0	0.0	0.0	5.389644369271803	0.0038238173535307717
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1965	0.0	74.51478	1
GTATCAA	4125	0.0	58.583042	1
TCAACGC	5275	0.0	45.00619	4
CAACGCA	5325	0.0	44.360123	5
AACGCAG	5560	0.0	42.3704	6
ATCAACG	5670	0.0	41.97358	3
TATCAAC	6075	0.0	39.86091	2
GTACATG	10105	0.0	37.75649	1
TACATGG	10425	0.0	36.24071	2
ACATGGG	10600	0.0	35.58627	3
ACGCAGA	6750	0.0	34.812523	7
CATGGGG	8855	0.0	32.5893	4
CGCAGAG	7280	0.0	32.11382	8
GTATAGG	515	0.0	30.049412	1
ATGGGGG	4595	0.0	29.782707	5
GCAGAGT	7965	0.0	28.977797	9
AGAGTAC	7015	0.0	25.65075	10-11
CAGAGTA	7450	0.0	25.151081	10-11
ATGGGTA	1220	0.0	23.89781	5
GTGGTAT	1035	0.0	23.578348	1
>>END_MODULE