FastQCFastQC Report
Mon 15 Aug 2016
SRR2921484_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921484_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909087
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29210.15300507520086828No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25790.1350907528048748No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28150.078.5847851
GTATCAA54750.057.9389271
TCAACGC73500.042.1286744
CAACGCA75800.040.9053275
ATCAACG78150.039.7743723
TATCAAC79550.039.226152
AACGCAG79850.038.7469376
GTACATG96400.033.15361
ACGCAGA93050.033.1172337
ACATGGG99950.031.6949923
CGCAGAG98800.030.9482048
TACATGG103100.030.9015082
GCAGAGT103300.029.3120889
GTGGTAT10850.028.5769941
TACGGTA1055.899588E-428.3394935
CATGGGG84950.028.1789264
AGAGTAC95250.027.32423810-11
ATGGGGG47450.026.9658185
CAGAGTA100550.025.7952310-11
AGTACTT56250.024.74894712-13
Produced by FastQC (version 0.11.5)