##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921484_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1909087
Sequences flagged as poor quality	0
Sequence length	125
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43547255834857	33.0	31.0	34.0	28.0	34.0
2	31.495454633549965	33.0	31.0	34.0	28.0	34.0
3	31.571386741410947	34.0	31.0	34.0	28.0	34.0
4	35.08366145702108	37.0	35.0	37.0	32.0	37.0
5	34.764922185316856	37.0	35.0	37.0	32.0	37.0
6	34.7428996164135	37.0	35.0	37.0	32.0	37.0
7	34.5945941698833	37.0	35.0	37.0	31.0	37.0
8	34.570059405359736	37.0	35.0	37.0	31.0	37.0
9	36.174796643631225	39.0	37.0	39.0	31.0	39.0
10-11	36.050627603666044	39.0	37.0	39.0	30.5	39.0
12-13	35.95775467540243	39.0	37.0	39.0	30.0	39.0
14-15	37.15588367633325	40.0	37.0	41.0	31.0	41.0
16-17	37.13606111193466	40.0	37.0	41.0	31.0	41.0
18-19	37.15130845267921	40.0	37.0	41.0	31.0	41.0
20-21	37.12894147830874	40.0	37.0	41.0	30.5	41.0
22-23	37.064689037220404	40.0	37.0	41.0	30.0	41.0
24-25	36.9946270128077	40.0	37.0	41.0	30.0	41.0
26-27	36.869107065314466	40.0	37.0	41.0	30.0	41.0
28-29	36.68162137189138	40.0	36.0	41.0	30.0	41.0
30-31	36.63683870876497	40.0	36.0	41.0	30.0	41.0
32-33	36.516623914991825	40.0	36.0	41.0	30.0	41.0
34-35	36.39011763214563	40.0	35.5	41.0	30.0	41.0
36-37	36.25415473469779	40.0	35.0	41.0	29.0	41.0
38-39	36.07986906830333	40.0	35.0	41.0	28.5	41.0
40-41	35.8777834640328	40.0	35.0	41.0	27.5	41.0
42-43	35.69756145214964	39.5	35.0	41.0	27.0	41.0
44-45	35.47762045417522	39.0	35.0	41.0	26.5	41.0
46-47	35.18997562709295	39.0	35.0	41.0	25.5	41.0
48-49	34.9312346687186	39.0	35.0	41.0	24.0	41.0
50-51	34.70202143747247	39.0	34.0	41.0	24.0	41.0
52-53	34.492621603939476	38.0	34.0	41.0	23.0	41.0
54-55	34.26662902214514	38.0	34.0	40.0	23.0	41.0
56-57	34.00659058492357	37.5	33.5	40.0	22.5	41.0
58-59	33.85233465001857	37.0	33.5	40.0	22.5	41.0
60-61	33.72494077011682	37.0	33.5	40.0	22.0	41.0
62-63	33.44789917903165	36.0	33.0	40.0	22.0	41.0
64-65	33.14273262559537	36.0	33.0	39.0	21.5	41.0
66-67	32.84253624900279	35.0	33.0	39.0	21.0	41.0
68-69	32.5274919372454	35.0	33.0	38.5	20.0	40.5
70-71	32.22389105368168	35.0	33.0	37.0	20.0	40.0
72-73	31.916354781107408	35.0	32.0	37.0	20.0	39.0
74-75	31.632729152731123	35.0	32.0	36.5	19.0	39.0
76-77	31.353285104345687	35.0	32.0	36.0	18.0	38.5
78-79	31.088446990629553	35.0	32.0	35.5	18.0	37.0
80-81	30.872145952489333	35.0	32.0	35.0	17.0	37.0
82-83	30.695592971928466	35.0	32.0	35.0	15.5	36.5
84-85	30.515051435581512	35.0	31.0	35.0	14.0	36.0
86-87	30.384602168471105	35.0	31.0	35.0	12.0	36.0
88-89	30.287239135775373	35.0	31.0	35.0	10.5	35.5
90-91	30.19536878099322	35.0	31.0	35.0	10.0	35.0
92-93	30.26393454043739	35.0	32.0	35.0	10.0	35.0
94-95	30.239249704177965	35.0	32.0	35.0	9.0	35.0
96-97	30.222764860899478	35.0	32.0	35.0	7.5	35.0
98-99	30.185203188749384	35.0	32.0	35.0	7.0	35.0
100-101	30.135934611675633	35.0	32.0	35.0	7.0	35.0
102-103	29.25420266336736	33.5	29.5	35.0	5.0	35.0
104-105	29.963507425277108	35.0	31.0	35.0	2.0	35.0
106-107	30.071434670080514	35.0	32.0	35.0	2.0	35.0
108-109	30.077548325456096	35.0	32.0	35.0	2.0	35.0
110-111	30.04780426455159	35.0	32.0	35.0	2.0	35.0
112-113	30.00148212208244	35.0	32.0	35.0	2.0	35.0
114-115	29.93694368040849	35.0	32.0	35.0	2.0	35.0
116-117	29.85928875949603	35.0	32.0	35.0	2.0	35.0
118-119	29.766725141389575	35.0	32.0	35.0	2.0	35.0
120-121	29.647037824886972	35.0	32.0	35.0	2.0	35.0
122-123	29.527707485305804	35.0	32.0	35.0	2.0	35.0
124-125	28.608597984272063	34.0	29.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	25907.0
3	10335.0
4	6190.0
5	5040.0
6	5510.0
7	6603.0
8	7507.0
9	8389.0
10	9453.0
11	10577.0
12	11162.0
13	13016.0
14	16301.0
15	13521.0
16	7689.0
17	6544.0
18	5802.0
19	5297.0
20	5142.0
21	5427.0
22	6505.0
23	8733.0
24	12341.0
25	16950.0
26	21286.0
27	23895.0
28	28113.0
29	33684.0
30	39236.0
31	47162.0
32	58560.0
33	75486.0
34	105955.0
35	172504.0
36	348068.0
37	554535.0
38	170373.0
39	289.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.49762262642876	17.116040794534715	12.1758973234749	25.210439255561628
2	17.299109715188482	19.37977593067135	36.94184338614139	26.379270967998774
3	19.48725068501045	23.665369119781214	28.69515332184227	28.15222687336606
4	13.663703641585778	15.072066500345919	34.71042369861003	36.55380615945827
5	14.66258067287914	36.70357962815291	33.01230094402594	15.621538754942016
6	34.35766512078226	34.22029586257626	16.379214595719443	15.042824420922042
7	30.17287083565588	31.149126373096735	19.938244699354158	18.739758091893226
8	28.541143265436975	32.870767128970954	18.85171619727311	19.736373408318965
9	27.948342135397077	14.078622456943924	18.391878144934946	39.581157262724055
10-11	26.926027580964835	24.37953829870325	26.28224822763581	22.412185892696108
12-13	27.204297752014405	21.994837082071328	26.74691120682159	24.053953959092674
14-15	24.516629536369795	23.038009096704474	24.279337867193142	28.16602349973259
16-17	24.510870134632512	26.08258683011158	24.773796427261775	24.632746607994132
18-19	24.587988720731236	24.839614057493257	26.848216516700724	23.72418070507478
20-21	25.57153622682757	23.8804296891064	26.637743773340073	23.910290310725962
22-23	25.38035061011944	23.49363871216826	26.537910383804114	24.588100293908184
24-25	24.791497498493857	24.083139062786493	26.528878644209865	24.596484794509784
26-27	24.527042654234503	24.106464416126506	26.669825521715296	24.696667407923698
28-29	24.662582150893083	24.179931738468753	26.651884526425807	24.50560158421236
30-31	24.813610683465477	24.130504087453993	26.52804399209622	24.52784123698431
32-33	24.486446870327704	24.18280982660837	26.485338354136346	24.84540494892758
34-35	24.719378386123815	24.261686826016955	26.588311785610063	24.430623002249163
36-37	24.888085367878915	24.084369356791445	26.3871520692616	24.64039320606804
38-39	24.89716176154312	24.30293579168875	26.236056292720473	24.56384615404766
40-41	24.91347133566102	24.196882505939268	26.29942296761756	24.59022319078215
42-43	24.897088621652827	24.257880355634903	26.127546198801	24.71748482391127
44-45	24.799884533152973	24.29566009158763	26.050059383698322	24.85439599156107
46-47	24.95932648696365	24.401435982601612	25.747230913703522	24.892006616731212
48-49	25.165387523124387	24.632668639738377	25.24793065852709	24.954013178610143
50-51	25.038485868869074	24.924148065490808	25.13550928781824	24.90185677782187
52-53	25.28809161929102	24.717671522899845	25.199129660744774	24.795107197064358
54-55	25.05112557467975	24.73796751381314	25.35909707795108	24.851809833556032
56-57	24.961813044103543	24.732835385732134	25.164385316404896	25.140966253759427
58-59	25.041217189309883	24.631821922882075	25.297190734868302	25.02977015293974
60-61	24.995376514158867	24.765957313044833	25.4842610255075	24.754405147288804
62-63	24.916834473423837	24.784728401914712	25.33830078008858	24.960136344572874
64-65	24.980347317451397	24.474498663408028	25.48150709421893	25.063646924921645
66-67	24.902402992346257	24.729106749019053	25.378941467988913	24.989548790645774
68-69	25.03319741755658	24.770918826452622	25.223304862187167	24.97257889380363
70-71	24.85088978915279	24.517700174400662	25.542336822219763	25.089073214226776
72-73	25.138518667538435	24.823760666566077	25.165630396150817	24.872090269744675
74-75	24.909980495119065	24.994433538545845	25.08831682255378	25.007269143781308
76-77	24.96782742876392	24.65557078581227	25.16084975926561	25.215752026158206
78-79	24.875108753429615	24.733798064038314	25.195166723821433	25.195926458710638
80-81	24.898255996714013	24.95410517668459	25.019463386828395	25.128175439773
82-83	25.01371852956222	24.82829901496374	24.994990608536945	25.162991846937093
84-85	24.791537116792124	24.891466236530523	24.955719481803996	25.361277164873357
86-87	24.64234627444745	24.970132767412252	25.06236037183362	25.325160586306676
88-89	24.74594217378252	24.782354029063473	25.024322595416088	25.44738120173792
90-91	24.692656068690393	24.733125355252234	25.136037048387642	25.43818152766973
92-93	24.7603835957875	24.9546278778786	24.98218248747405	25.30280603885986
94-95	24.64845949747375	24.625017352425804	25.19747715373071	25.529045996369742
96-97	24.663246795900974	24.701459739691018	25.121749739004805	25.5135437254032
98-99	24.63574948259795	24.652197393791802	25.006953694939437	25.705099428670803
100-101	24.562923645682776	24.79696177385971	25.107170341514934	25.532944238942584
102-103	24.65755541487922	24.525184628959764	25.202569994261488	25.61468996189953
104-105	24.489004304513912	24.74064878073694	25.102688913421257	25.667658001327894
106-107	24.52376942721902	24.722241937938186	25.06670095891713	25.687287675925663
108-109	24.57636109445519	24.627756511461413	25.043268287482178	25.752614106601218
110-111	24.617398886002377	24.71231418640642	25.032155700902614	25.638131226688586
112-113	24.61654475253225	24.670292266513282	25.093383686267483	25.619779294686985
114-115	24.281362449788414	24.860264011379503	25.159837646699547	25.69853589213254
116-117	24.374555218166716	24.841489380371268	24.99000100317375	25.793954398288264
118-119	24.342120772235724	24.550251218110546	25.116362887716953	25.99126512193678
120-121	24.06065431649306	24.614971595299146	25.287150703373207	26.03722338483459
122-123	24.125335110110978	24.667954201880075	25.15206325160785	26.0546474364011
124-125	24.152311482435834	24.737163641241075	25.052289581589037	26.05823529473405
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	38.0
1	39.5
2	70.0
3	101.0
4	76.5
5	65.5
6	120.5
7	252.5
8	444.5
9	516.0
10	433.0
11	396.5
12	469.0
13	575.0
14	690.0
15	821.5
16	966.0
17	1133.5
18	1336.5
19	1515.0
20	1736.5
21	1955.5
22	2177.5
23	2540.0
24	2932.5
25	3440.5
26	4404.5
27	5512.0
28	6296.0
29	7523.5
30	9474.5
31	11333.0
32	13408.0
33	15626.5
34	17977.0
35	20631.0
36	23902.5
37	27557.0
38	30515.5
39	33732.5
40	36942.5
41	39520.0
42	42819.5
43	48713.0
44	54696.0
45	58795.0
46	63232.0
47	67977.5
48	73424.0
49	78556.5
50	80087.5
51	80464.0
52	80936.5
53	81384.0
54	82707.5
55	81967.0
56	77862.0
57	72247.5
58	68403.0
59	64519.0
60	57909.0
61	51148.0
62	44150.5
63	34860.5
64	26648.0
65	21292.0
66	16710.0
67	13044.5
68	10786.5
69	8879.5
70	7608.5
71	6346.5
72	5060.5
73	4628.5
74	3844.0
75	3050.5
76	2145.5
77	1279.0
78	760.0
79	442.0
80	268.5
81	105.5
82	57.5
83	33.5
84	16.5
85	8.0
86	8.0
87	6.5
88	4.5
89	3.0
90	3.5
91	4.0
92	1.5
93	1.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12367168180391988
2	0.001833337087309274
3	0.018700038290554593
4	0.05903345421135862
5	0.023728620015745747
6	0.01901432464837904
7	0.013776218684638259
8	0.005185724904103375
9	0.003404768876431509
10-11	0.006573822984494683
12-13	0.0046619143077292965
14-15	0.0023047666240459445
16-17	0.009795258152195263
18-19	0.005133343844465967
20-21	0.01144526153077361
22-23	0.008250016892891733
24-25	0.012414311134065657
26-27	0.008773827489265812
28-29	0.01320002702862677
30-31	0.019930993192033678
32-33	0.011995262656966392
34-35	0.01980004054294016
36-37	0.013933361863550483
38-39	0.02019289849022072
40-41	0.009533352854008224
42-43	0.008538112720897476
44-45	0.016447652726146057
46-47	0.015164316765029566
48-49	0.020323851139314238
50-51	0.014116695572281408
52-53	0.021266710212787577
54-55	0.0160547947788655
56-57	0.02019289849022072
58-59	0.015504793652672716
60-61	0.018542895111642373
62-63	0.02061194696731998
64-65	0.01647384325596476
66-67	0.011366689941317499
68-69	0.022497665114266662
70-71	0.013828599744275668
72-73	0.016709558024333097
74-75	0.017495273918894216
76-77	0.012597644842796582
78-79	0.027578627899095223
80-81	0.019642897364027936
82-83	0.00903573278745285
84-85	0.012257167955153431
86-87	0.011654785769323241
88-89	0.01927622994656608
90-91	0.012152405835878616
92-93	0.007883349475429877
94-95	0.006914299872137834
96-97	0.002514290862595576
98-99	0.0015714317891222347
100-101	0.011000022523855643
102-103	0.0029071488098761345
104-105	0.0023309571538646483
106-107	3.4047688764315086E-4
108-109	0.01867384776073589
110-111	8.642874840172292E-4
112-113	0.008459541131441364
114-115	0.0021214329153150173
116-117	0.007621444177242838
118-119	0.009847639211832673
120-121	0.003771436293893364
122-123	6.809537752863017E-4
124-125	0.025404813924142795
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1909087.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.98610229006356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.08900787982353	42.93810175009556
2	11.15294731464758	12.265142037578032
3	3.9213692963537246	6.468624397392614
4	1.9333806302127992	4.252362603940342
5	1.1504488779881383	3.1629349842272316
6	0.7265650010120452	2.3970586479617078
7	0.5224738353171238	2.0110159826840435
8	0.4143161341458203	1.8225303466052623
9	0.29414214615138573	1.4556357122489032
>10	1.680889805855979	16.40717420610088
>50	0.08242288871757772	3.1017270679996822
>100	0.03069777618851287	2.967557643056814
>500	9.560097040964072E-4	0.3268631442072137
>1k	3.8240388163856263E-4	0.42327147590165376
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2921	0.15300507520086828	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2579	0.1350907528048748	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.238105963740783E-5
2	0.0	0.0	0.0	0.0	5.238105963740783E-5
3	0.0	0.0	0.0	0.0	5.238105963740783E-5
4	0.0	0.0	0.0	0.0	1.0476211927481566E-4
5	0.0	0.0	0.0	0.0	1.0476211927481566E-4
6	0.0	0.0	0.0	0.0	2.6190529818703913E-4
7	0.0	0.0	0.0	0.0	2.6190529818703913E-4
8	0.0	0.0	0.0	0.0	2.6190529818703913E-4
9	0.0	0.0	0.0	0.0	3.666674174618548E-4
10-11	0.0	0.0	0.0	0.0	3.666674174618548E-4
12-13	0.0	0.0	0.0	0.0	5.238105963740783E-4
14-15	0.0	0.0	0.0	5.238105963740783E-5	6.809537752863017E-4
16-17	0.0	0.0	0.0	5.238105963740783E-5	8.380969541985253E-4
18-19	0.0	0.0	0.0	1.0476211927481566E-4	8.380969541985253E-4
20-21	0.0	0.0	0.0	1.309526490935196E-4	8.380969541985253E-4
22-23	0.0	0.0	0.0	1.833337087309274E-4	8.904780138359332E-4
24-25	0.0	0.0	0.0	3.14286357824447E-4	9.952401331107487E-4
26-27	0.0	0.0	0.0	3.4047688764315086E-4	0.0010476211927481565
28-29	0.0	0.0	0.0	0.0011000022523855644	0.0010476211927481565
30-31	0.0	0.0	0.0	0.004426199539360962	0.0010738117225668605
32-33	0.0	0.0	0.0	0.010711926695849902	0.0011523833120229722
34-35	0.0	0.0	0.0	0.019119086767653858	0.00120476437166038
36-37	0.0	0.0	0.0	0.03265959068392378	0.00120476437166038
38-39	0.0	0.0	0.0	0.054214396724717104	0.00120476437166038
40-41	0.0	0.0	0.0	0.077576349323001	0.00120476437166038
42-43	0.0	0.0	0.0	0.10135735039838414	0.00120476437166038
44-45	0.0	0.0	0.0	0.1276526423363629	0.001283335961116492
46-47	0.0	0.0	0.0	0.15777175162787238	0.001519050729484827
48-49	0.0	0.0	0.0	0.19174086880273133	0.0015714317891222349
50-51	0.0	0.0	0.0	0.22835522948927944	0.0016238128487596427
52-53	0.0	0.0	0.0	0.26405292163217287	0.0017547654978531623
54-55	0.0	0.0	0.0	0.30137442662382596	0.0018595276171279778
56-57	0.0	0.0	0.0	0.3443530860563191	0.0019119086767653858
58-59	0.0	0.0	0.0	0.39458652224859314	0.0019380992065840897
60-61	0.0	0.0	0.0	0.4493247295696844	0.0019904802662214973
62-63	0.0	0.0	0.0	0.5059748455675409	0.002252385564408537
64-65	0.0	0.0	0.0	0.5660559209716477	0.0022785760942272406
66-67	0.0	0.0	0.0	0.6345965375071958	0.0023047666240459445
68-69	0.0	0.0	0.0	0.7159181325942715	0.0023047666240459445
70-71	0.0	0.0	0.0	0.8042849802025785	0.0023047666240459445
72-73	0.0	0.0	0.0	0.8957946913891299	0.0026190529818703916
74-75	0.0	0.0	0.0	0.9884829764175231	0.0026190529818703916
76-77	0.0	0.0	0.0	1.091437949134848	0.0026190529818703916
78-79	0.0	0.0	0.0	1.2092429522593784	0.0026190529818703916
80-81	0.0	0.0	0.0	1.3398551244652548	0.0026714340415077992
82-83	0.0	0.0	0.0	1.480655412770607	0.0028285772204200226
84-85	0.0	0.0	0.0	1.6213771294865031	0.0028547677502387264
86-87	0.0	0.0	0.0	1.777813164093622	0.0029071488098761345
88-89	0.0	0.0	0.0	1.9543897161313235	0.0029333393396948383
90-91	0.0	0.0	0.0	2.154249649177853	0.0029595298695135426
92-93	0.0	0.0	0.0	2.3679381819686585	0.0029857203993322464
94-95	0.0	0.0	0.0	2.5838005287344163	0.003090482518607062
96-97	0.0	0.0	0.0	2.814251000609192	0.003169054108063174
98-99	0.0	0.0	0.0	3.067225328128053	0.0032476256975192855
100-101	0.0	0.0	0.0	3.35160210089954	0.0032476256975192855
102-103	0.0	0.0	0.0	3.6664384598501796	0.0032476256975192855
104-105	0.0	0.0	0.0	3.992667699271956	0.0032738162273379893
106-107	0.0	0.0	0.0	4.326885050288436	0.003326197286975397
108-109	0.0	0.0	0.0	4.675245287407018	0.003430959406250213
110-111	0.0	0.0	0.0	5.069491332767967	0.003561912055343732
112-113	0.0	0.0	0.0	5.479215981251771	0.003561912055343732
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2815	0.0	78.584785	1
GTATCAA	5475	0.0	57.938927	1
TCAACGC	7350	0.0	42.128674	4
CAACGCA	7580	0.0	40.905327	5
ATCAACG	7815	0.0	39.774372	3
TATCAAC	7955	0.0	39.22615	2
AACGCAG	7985	0.0	38.746937	6
GTACATG	9640	0.0	33.1536	1
ACGCAGA	9305	0.0	33.117233	7
ACATGGG	9995	0.0	31.694992	3
CGCAGAG	9880	0.0	30.948204	8
TACATGG	10310	0.0	30.901508	2
GCAGAGT	10330	0.0	29.312088	9
GTGGTAT	1085	0.0	28.576994	1
TACGGTA	105	5.899588E-4	28.339493	5
CATGGGG	8495	0.0	28.178926	4
AGAGTAC	9525	0.0	27.324238	10-11
ATGGGGG	4745	0.0	26.965818	5
CAGAGTA	10055	0.0	25.79523	10-11
AGTACTT	5625	0.0	24.748947	12-13
>>END_MODULE