FastQCFastQC Report
Mon 15 Aug 2016
SRR2921483_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921483_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907032
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20200.071.286191
GTATCAA39850.056.8902741
TCAACGC50100.045.006424
CAACGCA51300.043.9524885
AACGCAG53800.041.6889276
ATCAACG54750.041.178563
TATCAAC58100.038.906622
GTACATG96850.038.3377271
TACATGG100850.036.157692
ACATGGG101050.035.3797043
ACGCAGA63850.035.032997
CGCAGAG69250.032.385388
CATGGGG83450.032.153134
GCAGAGT74950.029.1287549
ATGGGGG44000.027.4476385
AGAGTAC67500.025.558710-11
ATGGGGA34850.025.2650745
CAGAGTA74000.024.47936410-11
CATGGGT19500.020.7466184
GTGGTAT10650.020.6725231
Produced by FastQC (version 0.11.5)