##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921483_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1907032
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.081216256465545	31.0	30.0	34.0	26.0	34.0
2	30.09286682132235	31.0	30.0	34.0	26.0	34.0
3	30.234294967258023	31.0	31.0	34.0	26.0	34.0
4	33.63802862248772	37.0	35.0	37.0	28.0	37.0
5	33.646784637069544	37.0	35.0	37.0	28.0	37.0
6	33.66089085028463	37.0	35.0	37.0	28.0	37.0
7	33.48154671762194	37.0	35.0	37.0	27.0	37.0
8	33.42374538025581	37.0	35.0	37.0	27.0	37.0
9	34.90553802977611	39.0	35.0	39.0	27.0	39.0
10-11	34.813158877250096	39.0	35.0	39.0	27.0	39.0
12-13	34.69640362615834	39.0	35.0	39.0	26.5	39.0
14-15	35.87174677719095	40.0	36.0	41.0	27.0	41.0
16-17	35.80717523355665	40.0	36.0	41.0	26.0	41.0
18-19	35.82489412867744	40.0	36.0	41.0	26.0	41.0
20-21	35.855167349053396	40.0	36.0	41.0	26.0	41.0
22-23	35.819882151951305	40.0	36.0	41.0	26.0	41.0
24-25	35.77773917794772	40.0	36.0	41.0	26.0	41.0
26-27	35.7078937322499	40.0	36.0	41.0	26.0	41.0
28-29	35.67238069418866	40.0	35.5	41.0	26.0	41.0
30-31	35.578834807176804	40.0	35.0	41.0	26.0	41.0
32-33	35.479579000247504	40.0	35.0	41.0	25.0	41.0
34-35	35.315112174310656	40.0	35.0	41.0	25.0	41.0
36-37	35.178517717584185	40.0	35.0	41.0	24.5	41.0
38-39	35.055527909337656	40.0	35.0	41.0	24.0	41.0
40-41	34.911208883752344	39.0	35.0	41.0	24.0	41.0
42-43	34.714697236333734	39.0	34.0	41.0	23.0	41.0
44-45	34.52504231706652	39.0	34.0	41.0	22.0	41.0
46-47	34.282139995553294	39.0	33.5	41.0	20.0	41.0
48-49	34.1340378661711	39.0	33.0	41.0	19.5	41.0
50-51	33.94233867077217	38.5	33.0	41.0	18.0	41.0
52-53	33.757772811363424	38.0	33.0	40.0	17.5	41.0
54-55	33.56592679095054	38.0	33.0	40.0	16.0	41.0
56-57	33.33259405190894	37.5	33.0	40.0	14.5	41.0
58-59	33.096792555132794	37.0	33.0	40.0	13.5	41.0
60-61	32.795939973739294	36.5	32.0	40.0	11.5	41.0
62-63	32.49135961011666	36.0	32.0	39.5	8.0	41.0
64-65	32.190726479681516	35.5	32.0	39.0	8.0	41.0
66-67	32.15080266088876	35.0	32.0	39.0	7.5	41.0
68-69	31.907477955272906	35.0	32.0	39.0	7.0	41.0
70-71	31.605641384098433	35.0	32.0	37.5	3.5	40.0
72-73	31.271382965781385	35.0	31.0	37.0	2.0	39.5
74-75	30.96257115769426	35.0	31.0	37.0	2.0	39.0
76-77	30.67944402610968	35.0	31.0	36.0	2.0	39.0
78-79	30.397511420888584	35.0	31.0	36.0	2.0	37.5
80-81	30.150656622437378	35.0	31.0	35.0	2.0	37.0
82-83	29.943964233426605	35.0	31.0	35.0	2.0	37.0
84-85	29.73839138514718	35.0	30.5	35.0	2.0	36.0
86-87	29.57987149665029	35.0	30.0	35.0	2.0	36.0
88-89	29.443929624673316	35.0	30.0	35.0	2.0	36.0
90-91	29.330610603283006	35.0	30.0	35.0	2.0	35.0
92-93	29.231200630089063	34.0	30.0	35.0	2.0	35.0
94-95	29.160559183065622	34.0	30.0	35.0	2.0	35.0
96-97	29.058298707100874	34.0	30.0	35.0	2.0	35.0
98-99	28.998913232709256	34.0	30.0	35.0	2.0	35.0
100-101	28.930645107161286	34.0	30.0	35.0	2.0	35.0
102-103	27.95592811237567	33.5	28.0	34.5	2.0	35.0
104-105	28.772159565230158	34.0	29.5	35.0	2.0	35.0
106-107	29.09246305253399	35.0	30.0	35.0	2.0	35.0
108-109	29.129518277616736	35.0	31.0	35.0	2.0	35.0
110-111	29.068981537803246	35.0	30.0	35.0	2.0	35.0
112-113	28.998397509847763	35.0	30.0	35.0	2.0	35.0
114-115	28.88016588080326	35.0	30.0	35.0	2.0	35.0
116-117	28.81452906925526	35.0	30.0	35.0	2.0	35.0
118-119	28.65749971683747	35.0	30.0	35.0	2.0	35.0
120-121	28.4867736881185	35.0	30.0	35.0	2.0	35.0
122-123	28.354212724275207	35.0	30.0	35.0	2.0	35.0
124-125	27.51820367985435	34.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	66184.0
3	11895.0
4	7839.0
5	6662.0
6	7215.0
7	8386.0
8	9439.0
9	9558.0
10	10121.0
11	10868.0
12	10974.0
13	11580.0
14	13262.0
15	11569.0
16	8251.0
17	7008.0
18	6628.0
19	6157.0
20	5876.0
21	6160.0
22	7323.0
23	9960.0
24	14307.0
25	19480.0
26	23187.0
27	25188.0
28	29427.0
29	35060.0
30	39649.0
31	47181.0
32	59977.0
33	77595.0
34	108803.0
35	172652.0
36	329857.0
37	510105.0
38	161429.0
39	220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.763345784695474	17.315961226447804	12.076422442666644	24.844270546190078
2	16.682340661559724	19.48797700564631	38.19412500557288	25.635557327221086
3	19.076080457045027	24.201052395269258	29.13536161400844	27.587505533677277
4	13.19849647407855	15.125793883087537	35.986691508624396	35.68901813420952
5	14.08591445272995	37.447755149999374	33.71361219633992	14.752718200930756
6	33.30895603429959	34.9740969338639	17.35612978026331	14.360817251573202
7	29.654356957266874	31.325681054077776	20.731448223837823	18.288513764817527
8	27.741580135913825	33.65212670252917	19.56052256793724	19.045770593619764
9	27.678369576107205	14.617385516213652	19.012992217468184	38.691252690210966
10-11	26.202440643504847	25.004937467919575	27.136585968463677	21.6560359201119
12-13	26.426283942748785	22.700128781142872	27.22488439859731	23.648702877511035
14-15	23.868527007189382	23.96698723505373	24.461701358116425	27.702784399640468
16-17	24.039774233932107	26.95553896153799	25.005803097555773	23.99888370697413
18-19	24.09660393117467	26.115127370615426	26.558683659033775	23.22958503917613
20-21	25.206144876344556	25.09336235928748	26.191699846868676	23.50879291749929
22-23	24.891854953161193	24.794478503466674	26.046442690339216	24.267223853032917
24-25	24.417257168733126	25.26189222494462	26.080553848042825	24.240296758279424
26-27	24.122236363998837	25.311868145012784	26.21087942147659	24.355016069511787
28-29	24.137963597865166	25.29323009269728	26.302334036515457	24.2664722729221
30-31	24.45335911727426	25.286396907605912	26.060859474024394	24.199384501095434
32-33	24.06491562479833	25.387824765694393	26.077480480872804	24.46977912863447
34-35	24.334828148692555	25.348280962336894	26.22573416837355	24.091156720597002
36-37	24.47516841199555	25.202540345428222	25.991138695935028	24.331152546641206
38-39	24.458216030430414	25.448374523475405	25.959089784566675	24.134319661527506
40-41	24.46621317476856	25.252426685301593	26.15201317036165	24.129346969568196
42-43	24.320063759435108	25.31908575098352	26.009095898345887	24.35175459123549
44-45	24.272191308139238	25.408761538782404	25.94651005202503	24.37253710105333
46-47	24.407823037694794	25.37111575306419	25.843461588712813	24.377599620528205
48-49	24.481377107956824	25.496024173153142	25.649506592451583	24.373092126438458
50-51	24.452599837620816	25.677447787485256	25.508746571739987	24.361205803153936
52-53	24.526244679246272	25.5439991080154	25.683515993415057	24.246240219323273
54-55	24.426575031641626	25.490573090518005	25.743365095187194	24.339486782653175
56-57	24.345624996721174	25.618675144399504	25.547721346994233	24.48797851188509
58-59	24.435986977868094	25.416896297464064	25.68253792547712	24.46457879919072
60-61	24.46422599931021	25.5913374974264	25.751064518963325	24.193371984300065
62-63	24.264492378230713	25.647860225813684	25.74926523337447	24.338382162581134
64-65	24.31329501146902	25.413769903565818	25.814457651192345	24.458477433772817
66-67	24.23015579301422	25.72296126065423	25.647371525645823	24.399511420685727
68-69	24.395116979631702	25.59929670071437	25.658543161269442	24.347043158384487
70-71	24.218503257460576	25.398171960761477	25.897992010853656	24.48533277092429
72-73	24.428819499539582	25.600830245310004	25.691576036711655	24.27877421843876
74-75	24.275633926871194	25.78760531786704	25.559671250353443	24.37708950490832
76-77	24.253520838628003	25.538665951181795	25.677068211568454	24.53074499862175
78-79	24.283552377929958	25.608940481592125	25.679807593954003	24.427699546523918
80-81	24.287921340579587	25.717966262069915	25.51852208323051	24.47559031411999
82-83	24.408545869064806	25.69978271347496	25.52592202544817	24.36574939201206
84-85	24.207540406083996	25.71860063162328	25.484711732390608	24.589147229902114
86-87	24.07341606114666	25.764143128330208	25.662658595805464	24.49978221471767
88-89	24.253981457976238	25.567951754652814	25.62981619521365	24.5482505921573
90-91	24.158454785267384	25.61579932766587	25.635859218896677	24.589886668170067
92-93	24.249265530974622	25.81628138784816	25.548403101881075	24.386049979296143
94-95	24.19273294446758	25.516220862602278	25.652867561526417	24.638178631403722
96-97	24.225823368995176	25.559235368156074	25.637979861548143	24.576961401300608
98-99	24.13301038617568	25.62586744849457	25.543478688398206	24.697643476931542
100-101	24.111670335099113	25.688965176900258	25.626163920980026	24.57320056702061
102-103	24.205150041965656	25.48284101584941	25.764998100336328	24.547010841848607
104-105	24.006733388745698	25.66907806588128	25.711266626986518	24.612921918386505
106-107	24.04435446548275	25.679891948330912	25.64423133147042	24.63152225471592
108-109	24.074668445585637	25.626214091219946	25.604318493624838	24.694798969569575
110-111	24.11797913224695	25.700851139813107	25.62588706829125	24.555282659648693
112-113	24.055945779550072	25.754635053453757	25.705313700585563	24.48410546641061
114-115	23.946881608497684	25.935530561167564	25.630764510999033	24.48682331933572
116-117	23.85253937617452	25.95892118891675	25.606620813778207	24.581918621130523
118-119	24.156284265603038	25.631932624691313	25.545148332280288	24.66663477742536
120-121	23.81217512113681	25.785438848769427	25.736839404755486	24.665546625338283
122-123	23.873515920660275	25.872246568947567	25.604843468477327	24.64939404191483
124-125	23.854197256366152	25.91611780005873	25.667030247094853	24.562654696480262
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	98.0
1	119.5
2	169.0
3	192.0
4	166.5
5	154.0
6	157.5
7	193.0
8	266.0
9	319.0
10	339.5
11	373.0
12	455.0
13	559.0
14	627.0
15	663.0
16	732.0
17	877.5
18	1040.5
19	1142.5
20	1330.0
21	1532.5
22	1774.0
23	2235.0
24	2803.5
25	3672.0
26	5164.5
27	6760.0
28	7936.5
29	9626.5
30	12278.0
31	14844.5
32	17968.5
33	21485.0
34	24902.5
35	28401.5
36	32475.0
37	36583.0
38	38908.5
39	41319.5
40	44385.5
41	46917.0
42	49121.0
43	53787.0
44	59414.0
45	63201.0
46	66658.5
47	69561.0
48	72850.5
49	76504.0
50	76570.5
51	75313.5
52	74969.0
53	74919.0
54	75377.5
55	74422.5
56	70552.5
57	64624.0
58	60343.5
59	56682.5
60	51041.5
61	44956.0
62	38666.5
63	30673.0
64	23619.5
65	18816.0
66	14642.0
67	11386.5
68	9250.0
69	7497.5
70	6352.0
71	5239.0
72	4200.5
73	3812.0
74	3100.5
75	2398.0
76	1753.0
77	1050.0
78	611.5
79	392.0
80	266.0
81	135.0
82	80.0
83	56.0
84	45.5
85	30.0
86	22.0
87	20.0
88	14.0
89	10.5
90	9.5
91	9.0
92	8.0
93	9.0
94	10.5
95	8.5
96	9.5
97	10.5
98	6.5
99	7.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.035709940892444385
2	0.025012689876205536
3	0.027477252610338995
4	0.030413752889306526
5	0.032721003108495295
6	0.04651206691864636
7	0.0398000662810063
8	0.035500190872518135
9	0.030571065404251214
10-11	0.03620809718976924
12-13	0.03654894097214939
14-15	0.035578847129990473
16-17	0.03788609734917925
18-19	0.05036622353479124
20-21	0.06038178698626976
22-23	0.05385331761606517
24-25	0.06494384991966574
26-27	0.04918637967270607
28-29	0.04902906715776138
30-31	0.04908150466274295
32-33	0.056684942885069575
34-35	0.04071772261818365
36-37	0.05201800494171047
38-39	0.06164028710582728
40-41	0.04897662965277982
42-43	0.0586251305693874
44-45	0.05886109934180444
46-47	0.06423594360241464
48-49	0.04984184848497561
50-51	0.052411286229072186
52-53	0.0613256620759379
54-55	0.04821628583054715
56-57	0.04520112929410728
58-59	0.04680047319604495
60-61	0.03432034701043296
62-63	0.04271034780748304
64-65	0.042448160282575226
66-67	0.036103222179806105
68-69	0.03088569043414059
70-71	0.04960587971255857
72-73	0.0318557842762995
74-75	0.04084881638063756
76-77	0.03316672190083858
78-79	0.03392706572307124
80-81	0.0253535336585857
82-83	0.01746168915886047
84-85	0.01874640803090876
86-87	0.017697657931277505
88-89	0.02328225221181396
90-91	0.012689876205538238
92-93	0.020057345655447836
94-95	0.015206876444653263
96-97	0.012165501155722609
98-99	0.01145759483847151
100-101	0.011693563610888543
102-103	0.006056531825370524
104-105	0.006135188082842869
106-107	0.008730844579430234
108-109	0.013424001275280121
110-111	0.006213844340315212
112-113	0.007865625747234446
114-115	0.008363782044559294
116-117	0.007157719429983346
118-119	0.24194664798493157
120-121	0.35681100264704524
122-123	0.028683315224914945
124-125	0.0037755003586725337
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1907032.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.591947193072855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.45941586398543	47.142738454881226
2	10.36578445604971	12.1470299092728
3	3.4184877571668784	6.008875624442633
4	1.625148281371831	3.808824091322057
5	0.9495102372556476	2.781682684028249
6	0.6434992419068997	2.2622324160354887
7	0.43729566405196146	1.7935403119124578
8	0.33516771105672244	1.5710503061646865
9	0.25263436917654	1.3322105656152394
>10	1.4018749737535845	14.715459735493216
>50	0.08065388400153384	3.18942827504739
>100	0.029895565868421025	2.92254675415274
>500	4.514245391902606E-4	0.18017632889401505
>1k	1.8056981567610416E-4	0.14420454273784056
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	5.2437504981562974E-5
8	0.0	0.0	0.0	0.0	5.2437504981562974E-5
9	0.0	0.0	0.0	0.0	5.2437504981562974E-5
10-11	0.0	0.0	0.0	0.0	5.2437504981562974E-5
12-13	0.0	0.0	0.0	5.2437504981562974E-5	1.5731251494468891E-4
14-15	0.0	0.0	0.0	5.2437504981562974E-5	1.5731251494468891E-4
16-17	0.0	0.0	0.0	5.2437504981562974E-5	1.5731251494468891E-4
18-19	0.0	0.0	0.0	5.2437504981562974E-5	2.6218752490781485E-4
20-21	0.0	0.0	0.0	5.2437504981562974E-5	3.670625348709408E-4
22-23	0.0	0.0	0.0	1.0487500996312595E-4	4.195000398525038E-4
24-25	0.0	0.0	0.0	2.6218752490781485E-4	5.243750498156297E-4
26-27	0.0	0.0	0.0	4.719375448340667E-4	5.505938023064112E-4
28-29	0.0	0.0	0.0	0.0015206876444653263	5.768125547971927E-4
30-31	0.0	0.0	0.0	0.005401063013100987	5.768125547971927E-4
32-33	0.0	0.0	0.0	0.01182465737334245	5.768125547971927E-4
34-35	0.0	0.0	0.0	0.020529283200281905	6.292500597787557E-4
36-37	0.0	0.0	0.0	0.03424169075296062	6.292500597787557E-4
38-39	0.0	0.0	0.0	0.054220380150936116	6.554688122695372E-4
40-41	0.0	0.0	0.0	0.07658497602557272	6.816875647603186E-4
42-43	0.0	0.0	0.0	0.09832032184043057	6.816875647603186E-4
44-45	0.0	0.0	0.0	0.12595488696571427	7.079063172511001E-4
46-47	0.0	0.0	0.0	0.1562113273400761	7.603438222326632E-4
48-49	0.0	0.0	0.0	0.18817198662633872	7.865625747234446E-4
50-51	0.0	0.0	0.0	0.22417033379618173	7.865625747234446E-4
52-53	0.0	0.0	0.0	0.25943455589628284	8.390000797050076E-4
54-55	0.0	0.0	0.0	0.29831696584011175	8.914375846865706E-4
56-57	0.0	0.0	0.0	0.3451174390361567	8.914375846865706E-4
58-59	0.0	0.0	0.0	0.39773847528515516	8.914375846865706E-4
60-61	0.0	0.0	0.0	0.4526143242483608	8.914375846865706E-4
62-63	0.0	0.0	0.0	0.5113967673326929	8.914375846865706E-4
64-65	0.0	0.0	0.0	0.571621241804018	8.914375846865706E-4
66-67	0.0	0.0	0.0	0.6417564047168585	9.17656337177352E-4
68-69	0.0	0.0	0.0	0.7205961934566384	9.438750896681335E-4
70-71	0.0	0.0	0.0	0.8072229516861804	9.963125946496965E-4
72-73	0.0	0.0	0.0	0.8967337726897084	9.963125946496965E-4
74-75	0.0	0.0	0.0	0.9893121877346578	0.0010487500996312594
76-77	0.0	0.0	0.0	1.0911982599138348	0.0010487500996312594
78-79	0.0	0.0	0.0	1.2121453651538097	0.0010749688521220408
80-81	0.0	0.0	0.0	1.3449695652721088	0.0011011876046128225
82-83	0.0	0.0	0.0	1.487835547594377	0.0011011876046128225
84-85	0.0	0.0	0.0	1.6348703115626795	0.0011011876046128225
86-87	0.0	0.0	0.0	1.7931267015970367	0.0011011876046128225
88-89	0.0	0.0	0.0	1.9681893119779845	0.0011011876046128225
90-91	0.0	0.0	0.0	2.168421924750083	0.0011011876046128225
92-93	0.0	0.0	0.0	2.3818163512725534	0.0011011876046128225
94-95	0.0	0.0	0.0	2.6041513723943805	0.0011011876046128225
96-97	0.0	0.0	0.0	2.8333032691638103	0.0011536251095943854
98-99	0.0	0.0	0.0	3.0826436053511426	0.0012060626145759484
100-101	0.0	0.0	0.0	3.366592694826306	0.0012060626145759484
102-103	0.0	0.0	0.0	3.6708088799768435	0.0012060626145759484
104-105	0.0	0.0	0.0	3.9928013793161306	0.0012060626145759484
106-107	0.0	0.0	0.0	4.323970442027192	0.0012060626145759484
108-109	0.0	0.0	0.0	4.668983006053385	0.0012585001195575113
110-111	0.0	0.0	0.0	5.053481011330696	0.0013109376245390744
112-113	0.0	0.0	0.0	5.468523863259767	0.0013633751295206373
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2020	0.0	71.28619	1
GTATCAA	3985	0.0	56.890274	1
TCAACGC	5010	0.0	45.00642	4
CAACGCA	5130	0.0	43.952488	5
AACGCAG	5380	0.0	41.688927	6
ATCAACG	5475	0.0	41.17856	3
TATCAAC	5810	0.0	38.90662	2
GTACATG	9685	0.0	38.337727	1
TACATGG	10085	0.0	36.15769	2
ACATGGG	10105	0.0	35.379704	3
ACGCAGA	6385	0.0	35.03299	7
CGCAGAG	6925	0.0	32.38538	8
CATGGGG	8345	0.0	32.15313	4
GCAGAGT	7495	0.0	29.128754	9
ATGGGGG	4400	0.0	27.447638	5
AGAGTAC	6750	0.0	25.5587	10-11
ATGGGGA	3485	0.0	25.265074	5
CAGAGTA	7400	0.0	24.479364	10-11
CATGGGT	1950	0.0	20.746618	4
GTGGTAT	1065	0.0	20.672523	1
>>END_MODULE