FastQCFastQC Report
Mon 15 Aug 2016
SRR2921483_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921483_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907032
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30120.1579417650044677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26270.13775332558656594No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30550.076.6887741
GTATCAA57200.056.487681
TCAACGC72250.043.59574
CAACGCA73900.042.5183145
ATCAACG76900.041.1918333
AACGCAG77850.040.503256
TATCAAC79650.040.0686652
GTACATG96800.035.8424841
ACATGGG98550.034.257233
TACATGG101650.033.9154052
ACGCAGA93050.033.8220987
CGCAGAG97800.032.179418
CATGGGG82500.030.601154
GCAGAGT105000.029.292919
CAGAGTA102400.026.2025510-11
GAGTACT57000.026.19717412-13
ATGGGGG46350.026.0635075
AGAGTAC98050.024.9986510-11
GTACTTT61550.024.40652714-15
AGTACTT63600.022.40288712-13
Produced by FastQC (version 0.11.5)