FastQCFastQC Report
Mon 15 Aug 2016
SRR2921482_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921482_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978670
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21750.069.493891
GTATCAA44550.060.7765241
TCAACGC55700.047.853984
CAACGCA56500.046.9657945
ATCAACG59450.044.929843
AACGCAG59300.044.543026
TATCAAC63450.042.566172
GTACATG100300.039.2761761
TACATGG104450.037.305532
ACGCAGA70350.037.2928737
ACATGGG106500.036.252293
CGCAGAG75650.034.757048
CATGGGG89650.032.784754
GCAGAGT82350.030.5559069
ATGGGGG48550.028.3085775
AGAGTAC72950.026.58341210-11
CAGAGTA79000.025.37588910-11
ATGGGGA36300.022.7826085
CATGGGT19000.021.6068154
GTCTTAG7750.020.7316681
Produced by FastQC (version 0.11.5)