##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921482_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1978670
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.59710158844072	31.0	30.0	34.0	25.0	34.0
2	29.678730157125745	31.0	30.0	34.0	25.0	34.0
3	29.82719048653894	31.0	30.0	34.0	25.0	34.0
4	33.17965047228694	37.0	35.0	37.0	26.0	37.0
5	33.20268918010583	37.0	35.0	37.0	26.0	37.0
6	33.22514315171302	37.0	35.0	37.0	26.0	37.0
7	33.062661787968686	37.0	35.0	37.0	26.0	37.0
8	33.00087836779251	37.0	35.0	37.0	25.0	37.0
9	34.439338545588704	39.0	35.0	39.0	25.0	39.0
10-11	34.31386385804606	39.0	35.0	39.0	25.0	39.0
12-13	34.157348370369995	39.0	35.0	39.0	23.0	39.0
14-15	35.27998782010138	40.0	36.0	41.0	23.5	41.0
16-17	35.19256748219763	40.0	35.0	41.0	23.0	41.0
18-19	35.20290776127399	40.0	35.0	41.0	23.0	41.0
20-21	35.21235602702825	40.0	35.0	41.0	23.0	41.0
22-23	35.172838826080145	40.0	35.0	41.0	23.0	41.0
24-25	35.12671314569888	40.0	35.0	41.0	23.0	41.0
26-27	35.038963546220444	40.0	35.0	41.0	23.0	41.0
28-29	34.98068980678941	40.0	35.0	41.0	23.0	41.0
30-31	34.881625789040115	40.0	34.5	41.0	23.0	41.0
32-33	34.75812566016567	39.0	34.0	41.0	22.5	41.0
34-35	34.57949102174693	39.0	34.0	41.0	21.0	41.0
36-37	34.4339392116927	39.0	34.0	41.0	19.0	41.0
38-39	34.30456746198204	39.0	34.0	41.0	18.5	41.0
40-41	34.13833357760515	39.0	33.0	41.0	16.5	41.0
42-43	33.93642017112505	39.0	33.0	41.0	15.0	41.0
44-45	33.74112863691268	38.5	33.0	41.0	14.5	41.0
46-47	33.47688194595359	38.0	33.0	41.0	9.0	41.0
48-49	33.32329039203101	38.0	33.0	40.0	8.5	41.0
50-51	33.12113364027351	38.0	33.0	40.0	7.5	41.0
52-53	32.925828713226565	38.0	32.0	40.0	6.5	41.0
54-55	32.716707434792056	37.5	32.0	40.0	2.0	41.0
56-57	32.471814400582204	37.0	32.0	40.0	2.0	41.0
58-59	32.21956213011771	36.5	31.5	40.0	2.0	41.0
60-61	31.907026942340057	36.0	31.0	39.5	2.0	41.0
62-63	31.5945271318613	35.5	31.0	39.0	2.0	41.0
64-65	31.285009627679198	35.0	31.0	39.0	2.0	41.0
66-67	31.236875274805804	35.0	31.0	39.0	2.0	40.5
68-69	30.99735377804283	35.0	31.0	38.0	2.0	40.0
70-71	30.694109174344383	35.0	31.0	37.0	2.0	40.0
72-73	30.374258213850716	35.0	30.0	37.0	2.0	39.0
74-75	30.083254660959128	35.0	30.0	36.0	2.0	39.0
76-77	29.80977601115901	35.0	30.0	36.0	2.0	38.5
78-79	29.540757680664285	35.0	30.0	35.0	2.0	37.0
80-81	29.31305649754633	35.0	30.0	35.0	2.0	37.0
82-83	29.12454502266674	35.0	30.0	35.0	2.0	36.0
84-85	28.931533302673007	34.0	29.0	35.0	2.0	36.0
86-87	28.787722055724302	34.0	29.0	35.0	2.0	36.0
88-89	28.667624717613347	34.0	29.0	35.0	2.0	35.0
90-91	28.561837244209492	34.0	29.0	35.0	2.0	35.0
92-93	28.465371436368876	34.0	29.0	35.0	2.0	35.0
94-95	28.396065538973147	34.0	29.0	35.0	2.0	35.0
96-97	28.305106460400168	34.0	29.0	35.0	2.0	35.0
98-99	28.23793709916257	34.0	29.0	35.0	2.0	35.0
100-101	28.175716769345065	34.0	29.0	35.0	2.0	35.0
102-103	27.215755785451847	32.5	26.0	34.5	2.0	35.0
104-105	28.01642113136602	34.0	28.5	35.0	2.0	35.0
106-107	28.34041502625501	34.0	29.0	35.0	2.0	35.0
108-109	28.380355744009865	34.0	29.0	35.0	2.0	35.0
110-111	28.316108294965808	34.0	29.0	35.0	2.0	35.0
112-113	28.24021564990625	34.0	29.0	35.0	2.0	35.0
114-115	28.118825776910754	34.0	29.0	35.0	2.0	35.0
116-117	28.04901524761582	34.0	29.0	35.0	2.0	35.0
118-119	27.88633450752273	34.0	29.0	35.0	2.0	35.0
120-121	27.705575715000478	34.0	29.0	35.0	2.0	35.0
122-123	27.571397453845258	34.0	29.0	35.0	2.0	35.0
124-125	26.72994157691783	33.5	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	84976.0
3	15150.0
4	9999.0
5	8453.0
6	8900.0
7	10317.0
8	11196.0
9	11541.0
10	12238.0
11	12883.0
12	13180.0
13	13658.0
14	14916.0
15	12919.0
16	9166.0
17	7747.0
18	7114.0
19	6713.0
20	6534.0
21	6872.0
22	8312.0
23	11273.0
24	16392.0
25	21809.0
26	25549.0
27	27577.0
28	32552.0
29	38010.0
30	43385.0
31	51783.0
32	65202.0
33	84282.0
34	117093.0
35	183540.0
36	345412.0
37	490220.0
38	131677.0
39	130.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.25844493394966	16.820604652973444	12.03835667133983	24.88259374173707
2	16.88858175707817	18.87158716766448	38.09617932633322	26.143651748924125
3	19.43806922044098	23.54823511331834	28.499138586472977	28.514557079767698
4	13.546967995749416	14.668408422102027	35.20735500720142	36.577268574947134
5	14.730169836331447	36.84797236835586	33.17485852027935	15.24699927503334
6	34.3746476494712	33.93825124924853	16.939218490951262	14.747882610329007
7	30.55972803574688	30.872735674629798	20.07763762401367	18.48989866560966
8	28.245404008436687	33.1693484622058	19.186331032725523	19.398916496631998
9	27.81681027074137	14.644835207499632	18.659851455217165	38.87850306654184
10-11	26.771165896951725	24.59483166119398	26.469473029643503	22.164529412210786
12-13	26.882099328413894	22.19406533050489	26.792665737784148	24.13116960329706
14-15	24.264960518659286	23.399753187905297	24.04875979702966	28.286526496405756
16-17	24.451740859904106	26.496672376172153	24.588698212527074	24.462888551396674
18-19	24.619214971152438	25.516483977664215	26.205988855368307	23.658312195815036
20-21	25.54033286962379	24.489856290495574	25.945029433166916	24.024781406713725
22-23	25.27462608908026	24.16944712229479	25.82520560454905	24.730721184075897
24-25	24.83880674002751	24.70990270248402	25.794132818189176	24.657157739299297
26-27	24.582601970867525	24.643706499049305	25.96947503954603	24.80421649053714
28-29	24.51178180490688	24.791998798636637	25.98802782753964	24.708191568916845
30-31	24.903071476316978	24.655769750008343	25.814411961900063	24.626746811774616
32-33	24.510690563880566	24.691565595516	25.81934189704383	24.978401943559607
34-35	24.820563715459162	24.795790347537487	25.872976831086834	24.510669105916513
36-37	24.910591172733355	24.61016841169573	25.687144696930243	24.792095718640674
38-39	24.871613279552207	24.854976292293156	25.644778071945108	24.62863235620953
40-41	24.90769870186605	24.69905377896835	25.800677735743733	24.592569783421865
42-43	24.75080729738176	24.76994679371719	25.689401118333162	24.789844790567894
44-45	24.75534808269507	24.7322640159854	25.635096276739784	24.877291624579755
46-47	24.850103858373338	24.89250689966004	25.50723721555891	24.750152026407715
48-49	24.99268742872297	24.980173261801177	25.193091067488776	24.834048241987077
50-51	24.862031818955206	25.2172134535694	25.107111950348898	24.813642777126496
52-53	25.036288965600768	25.0888798371908	25.230925758629642	24.64390543857879
54-55	24.907358203317965	24.96042277726682	25.33361917585332	24.798599843561895
56-57	24.76602661738738	25.065848266482288	25.226806482315954	24.941318633814372
58-59	24.90913554067545	24.93798059822273	25.257298671736926	24.895585189364898
60-61	24.89202017258067	24.994773844602573	25.424317054607066	24.68888892820969
62-63	24.75032345581763	25.09923609316943	25.341164360047912	24.809276090965028
64-65	24.862706534269016	24.760880336521932	25.434707869031488	24.941705260177564
66-67	24.712002578345686	25.131742089597513	25.295593430274078	24.860661901782724
68-69	24.866558128636523	25.070692532790794	25.24939860477825	24.813350733794433
70-71	24.728150126457088	24.920085794636925	25.44393971723366	24.90782436167233
72-73	24.99903315067254	25.143567646213526	25.196270413475325	24.66112878963861
74-75	24.71745771092463	25.29361444820707	25.200537530574834	24.78839031029347
76-77	24.78465239795539	24.981737350492725	25.275026654621325	24.958583596930563
78-79	24.747290913089305	25.0418970054068	25.25369489880262	24.95711718270128
80-81	24.769145143214182	25.2288580829657	25.10809118027531	24.89390559354481
82-83	24.907756398542908	25.224792835744847	25.073250227528742	24.794200538183507
84-85	24.74139726354227	25.228804786273635	25.028382610735143	25.001415339448947
86-87	24.534633131457216	25.285356521580898	25.207362568513243	24.972647778448646
88-89	24.710507073573098	25.12744260249054	25.121250240427933	25.040800083508426
90-91	24.663364907865564	25.101285583266215	25.173034013406014	25.062315495462208
92-93	24.703231546117646	25.299043122665303	25.134508747365174	24.863216583851873
94-95	24.656936000054593	25.004467028527564	25.29477965481432	25.043817316603523
96-97	24.696046154938685	25.14758273642743	25.141011990852512	25.015359117781372
98-99	24.650479854937863	25.09756945102573	25.101789899985466	25.150160794050937
100-101	24.5534276929671	25.19182858982673	25.211869439801383	25.042874277404785
102-103	24.722948270620527	25.005780729536912	25.318432908031692	24.95283809181087
104-105	24.458345789874432	25.226511091102022	25.21198021475877	25.103162904264774
106-107	24.47636321455531	25.23090157693135	25.19938771626608	25.093347492247265
108-109	24.594481414115833	25.20835402531359	25.13385026006818	25.06331430050239
110-111	24.585618884103685	25.245141272434957	25.14345079459585	25.02578904886551
112-113	24.590081490904993	25.292001073538934	25.191571607422382	24.926345828133694
114-115	24.350907235060294	25.513195146957703	25.21403085097054	24.921866767011462
116-117	24.384899921380608	25.52047124124882	25.13119026625717	24.9634385711134
118-119	24.590871096025737	25.182172800729504	25.12702956001925	25.099926543225514
120-121	24.280567466603912	25.28430768653361	25.306623731947774	25.1285011149147
122-123	24.30690592938846	25.38265799405441	25.226978655576588	25.083457420980537
124-125	24.317005483161605	25.480578974154998	25.22229067680581	24.980124865877592
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	113.0
1	136.0
2	173.0
3	175.5
4	152.0
5	149.0
6	161.5
7	189.5
8	264.5
9	339.0
10	390.5
11	433.0
12	477.0
13	571.0
14	661.5
15	725.5
16	826.0
17	984.0
18	1140.5
19	1230.5
20	1330.0
21	1545.0
22	1841.0
23	2171.5
24	2506.0
25	3216.5
26	4484.5
27	5812.0
28	6821.0
29	8229.0
30	10376.0
31	12426.5
32	14923.5
33	17818.5
34	20943.0
35	24336.5
36	28304.0
37	32059.0
38	34583.5
39	37596.5
40	40458.0
41	42591.5
42	45461.0
43	51459.5
44	57485.0
45	61580.5
46	65512.5
47	69446.5
48	75584.5
49	81215.5
50	81560.0
51	80746.0
52	80721.5
53	81274.5
54	83342.5
55	83602.5
56	80155.5
57	74319.5
58	70402.5
59	66255.5
60	59346.5
61	52328.5
62	45273.5
63	36036.0
64	27370.5
65	21675.5
66	16855.5
67	12983.0
68	10503.5
69	8508.0
70	7230.0
71	6030.0
72	4802.0
73	4374.0
74	3617.5
75	2793.0
76	1985.5
77	1132.5
78	686.5
79	491.0
80	316.5
81	167.0
82	96.5
83	50.5
84	38.5
85	27.0
86	18.5
87	18.0
88	17.5
89	11.0
90	9.5
91	10.5
92	11.5
93	10.5
94	9.0
95	12.0
96	12.5
97	9.5
98	7.5
99	6.5
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.035832149878453706
2	0.0258254281916641
3	0.026583513167936036
4	0.029767470068278186
5	0.03274927097494782
6	0.044524857606371954
7	0.0385612557930327
8	0.03244603698443904
9	0.027594293136298624
10-11	0.033608433948056016
12-13	0.033658972946474144
14-15	0.03269873197652969
16-17	0.03446759692116422
18-19	0.047835162002759425
20-21	0.057563919198249325
22-23	0.051878281876209784
24-25	0.06216296805429909
26-27	0.0460662970581249
28-29	0.046344261549424615
30-31	0.046698034538351516
32-33	0.0536471468208443
34-35	0.03737358933020665
36-37	0.04836582148614979
38-39	0.05804403968322156
40-41	0.04588941056366145
42-43	0.05478427428525221
44-45	0.056376252735423286
46-47	0.06153123057407248
48-49	0.0460662970581249
50-51	0.04798677899801382
52-53	0.057639727695876525
54-55	0.045030247590553255
56-57	0.041821021191002034
58-59	0.04353934713721844
60-61	0.03226915048997559
62-63	0.0409871277171029
64-65	0.039925808750322186
66-67	0.033229391459920044
68-69	0.02837764761177963
70-71	0.046698034538351516
72-73	0.03009597355799603
74-75	0.0371967028357432
76-77	0.029767470068278186
78-79	0.030576094042968256
80-81	0.024208180242283956
82-83	0.01761284094871808
84-85	0.017486493452672752
86-87	0.01574289800724729
88-89	0.021554882825332168
90-91	0.011598700136960686
92-93	0.018194039430526568
94-95	0.01442888404837593
96-97	0.010284686178089322
98-99	0.01048684217176184
100-101	0.010663728666225293
102-103	0.005736176320457681
104-105	0.006292105303057104
106-107	0.008869594222381701
108-109	0.012457863110068886
110-111	0.005913062814921134
112-113	0.008440012735827602
114-115	0.008010431249273501
116-117	0.007606119261928467
118-119	0.23854407253357052
120-121	0.3534950244356057
122-123	0.028579803605452148
124-125	0.0033861128940146667
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1978670.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.87513160844414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.94575574255235	44.66979623651641
2	10.260635095955383	11.466286727454557
3	3.4571740490822873	5.795101649573114
4	1.781440515572501	3.981528930409123
5	1.0122533096111541	2.827989344780319
6	0.6741088782076596	2.259955337296417
7	0.48739931210025883	1.9063450496626968
8	0.3567340804504314	1.5946050955508124
9	0.2692929227480713	1.3542099749793959
>10	1.6219162454817737	16.459554571373836
>50	0.09499776550097394	3.6197021223163968
>100	0.03692834954594424	3.4345579226924188
>500	0.0010909865530614247	0.3915600873750643
>1k	2.7274663826535597E-4	0.23880695001948313
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	1.5161699525438805E-4
14-15	0.0	0.0	0.0	0.0	2.2742549288158208E-4
16-17	0.0	0.0	0.0	0.0	2.5269499209064675E-4
18-19	0.0	0.0	0.0	0.0	2.7796449129971143E-4
20-21	0.0	0.0	0.0	0.0	3.285034897178408E-4
22-23	0.0	0.0	0.0	5.053899841812935E-5	4.043119873450348E-4
24-25	0.0	0.0	0.0	1.010779968362587E-4	5.306594833903582E-4
26-27	0.0	0.0	0.0	2.2742549288158208E-4	5.811984818084875E-4
28-29	0.0	0.0	0.0	0.001389822456498557	6.064679810175522E-4
30-31	0.0	0.0	0.0	0.004270545366331931	6.064679810175522E-4
32-33	0.0	0.0	0.0	0.009804565693117093	6.064679810175522E-4
34-35	0.0	0.0	0.0	0.01660206098035549	6.570069794356816E-4
36-37	0.0	0.0	0.0	0.027644832134716753	6.570069794356816E-4
38-39	0.0	0.0	0.0	0.045712524069198	8.086239746900696E-4
40-41	0.0	0.0	0.0	0.06633243542379477	8.59162973108199E-4
42-43	0.0	0.0	0.0	0.0866996517863009	8.59162973108199E-4
44-45	0.0	0.0	0.0	0.11057932853886701	8.59162973108199E-4
46-47	0.0	0.0	0.0	0.13898224564985573	9.097019715263283E-4
48-49	0.0	0.0	0.0	0.1692298362031061	9.855104691535222E-4
50-51	0.0	0.0	0.0	0.20402593661398818	0.001010779968362587
52-53	0.0	0.0	0.0	0.23667412959209974	0.0012382054612441692
54-55	0.0	0.0	0.0	0.2739971799238883	0.0013140139588713631
56-57	0.0	0.0	0.0	0.31607089610698097	0.0013140139588713631
58-59	0.0	0.0	0.0	0.36458833458838513	0.0013140139588713631
60-61	0.0	0.0	0.0	0.41730050993849405	0.0013140139588713631
62-63	0.0	0.0	0.0	0.4726912522047638	0.0015667089509620099
64-65	0.0	0.0	0.0	0.5289411574441418	0.0016172479493801392
66-67	0.0	0.0	0.0	0.5940606569059015	0.001642517448589204
68-69	0.0	0.0	0.0	0.6679234030939976	0.0016677869477982686
70-71	0.0	0.0	0.0	0.7508831689973567	0.001718325946216398
72-73	0.0	0.0	0.0	0.8319224529608272	0.0021479074327704975
74-75	0.0	0.0	0.0	0.9210227071719893	0.0022995244280248853
76-77	0.0	0.0	0.0	1.022100704008248	0.0023753329256520793
78-79	0.0	0.0	0.0	1.1353333299640669	0.0023753329256520793
80-81	0.0	0.0	0.0	1.2615039395149266	0.002400602424861144
82-83	0.0	0.0	0.0	1.3919956334305366	0.0025269499209064675
84-85	0.0	0.0	0.0	1.5282993121642314	0.0025774889193245967
86-87	0.0	0.0	0.0	1.6736747411139805	0.0026785669161608554
88-89	0.0	0.0	0.0	1.8451282932474844	0.002779644912997114
90-91	0.0	0.0	0.0	2.03651948025694	0.002779644912997114
92-93	0.0	0.0	0.0	2.240317991378047	0.002779644912997114
94-95	0.0	0.0	0.0	2.4552603516503506	0.0029312619082515023
96-97	0.0	0.0	0.0	2.677151824205148	0.0030576094042968254
98-99	0.0	0.0	0.0	2.920572910086068	0.0031334179019240197
100-101	0.0	0.0	0.0	3.2007863868153863	0.0031334179019240197
102-103	0.0	0.0	0.0	3.4899199967655044	0.003183956900342149
104-105	0.0	0.0	0.0	3.794973391217333	0.0032344958987602784
106-107	0.0	0.0	0.0	4.112055067292676	0.0032597653979693428
108-109	0.0	0.0	0.0	4.445814612846002	0.0033355738955965367
110-111	0.0	0.0	0.0	4.821395179590331	0.00346192139164186
112-113	0.0	0.0	0.0	5.219743565122027	0.003487190890850925
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2175	0.0	69.49389	1
GTATCAA	4455	0.0	60.776524	1
TCAACGC	5570	0.0	47.85398	4
CAACGCA	5650	0.0	46.965794	5
ATCAACG	5945	0.0	44.92984	3
AACGCAG	5930	0.0	44.54302	6
TATCAAC	6345	0.0	42.56617	2
GTACATG	10030	0.0	39.276176	1
TACATGG	10445	0.0	37.30553	2
ACGCAGA	7035	0.0	37.292873	7
ACATGGG	10650	0.0	36.25229	3
CGCAGAG	7565	0.0	34.75704	8
CATGGGG	8965	0.0	32.78475	4
GCAGAGT	8235	0.0	30.555906	9
ATGGGGG	4855	0.0	28.308577	5
AGAGTAC	7295	0.0	26.583412	10-11
CAGAGTA	7900	0.0	25.375889	10-11
ATGGGGA	3630	0.0	22.782608	5
CATGGGT	1900	0.0	21.606815	4
GTCTTAG	775	0.0	20.731668	1
>>END_MODULE