FastQCFastQC Report
Mon 15 Aug 2016
SRR2921482_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921482_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978670
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29250.14782657037302835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25140.1270550420231772No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29800.080.828691
GTATCAA59700.059.221691
TCAACGC76450.045.4857374
CAACGCA79300.043.897225
AACGCAG82050.042.4898876
ATCAACG82150.042.3296933
TATCAAC82800.042.142292
GTACATG100050.036.4103281
ACGCAGA97200.035.8663257
TACATGG106200.034.2584342
ACATGGG105000.034.1954153
CGCAGAG102450.034.0855838
GCAGAGT111100.031.0033829
CATGGGG85650.030.7975524
CAGAGTA107400.027.14210-11
ATGGGGG45000.026.3144865
AGAGTAC102900.026.13202910-11
GAGTACT59800.025.9156512-13
GTGGTAT11000.024.9324871
GTACTTT62800.024.58352914-15
Produced by FastQC (version 0.11.5)