FastQCFastQC Report
Mon 15 Aug 2016
SRR2921481_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921481_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2108412
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24200.072.047781
GTATCAA46100.059.119791
TCAACGC57250.046.5584684
CAACGCA58750.045.2663275
AACGCAG61700.043.1964236
ATCAACG65150.041.275213
TATCAAC65200.041.060092
GTACATG103650.036.743371
ACGCAGA73900.035.58227
TACATGG109050.034.3692322
ACATGGG111900.033.388343
CGCAGAG79400.032.813848
CATGGGG94800.030.6899874
GCAGAGT85100.030.0574869
ATGGGGG49200.026.7240775
CAGAGTA84350.025.35379810-11
GTATAGG5650.025.2773271
AGAGTAC76950.024.9702610-11
ATGGGGA37450.020.81115
GAGTACA74050.020.00457212-13
Produced by FastQC (version 0.11.5)