FastQCFastQC Report
Mon 15 Aug 2016
SRR2921481_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921481_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2108412
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29010.1375917040881953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26060.12360013128363907No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29150.075.857051
GTATCAA57850.060.683771
TCAACGC76500.045.219064
CAACGCA77700.044.4964035
AACGCAG81000.042.8213736
TATCAAC83850.041.6823462
ATCAACG83400.041.6206933
ACGCAGA97450.035.224127
CGCAGAG104000.033.0620968
GTACATG103100.032.8360021
TACATGG108650.030.7432822
GCAGAGT112850.029.8887029
ACATGGG111650.029.596553
GTGGTAT11050.026.9692171
CATGGGG93500.026.9361534
AGAGTAC102450.026.79726210-11
CAGAGTA108450.025.61639410-11
GTATAGG6850.024.362851
GAGTACT57950.022.89218712-13
ATGGGGG49300.022.0888925
Produced by FastQC (version 0.11.5)