##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921481_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2108412 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.274567304682385 31.0 31.0 34.0 28.0 34.0 2 31.338350379337626 33.0 31.0 34.0 28.0 34.0 3 31.420344790297154 33.0 31.0 34.0 28.0 34.0 4 34.93249374410694 37.0 35.0 37.0 32.0 37.0 5 34.5830473360994 37.0 35.0 37.0 31.0 37.0 6 34.554240347711925 37.0 35.0 37.0 31.0 37.0 7 34.397159568433494 37.0 35.0 37.0 30.0 37.0 8 34.373768978738504 37.0 35.0 37.0 30.0 37.0 9 35.95535502548838 39.0 37.0 39.0 30.0 39.0 10-11 35.82190577553153 39.0 37.0 39.0 30.0 39.0 12-13 35.73403300683168 39.0 37.0 39.0 30.0 39.0 14-15 36.919852476650675 40.0 37.0 41.0 30.0 41.0 16-17 36.89546279379931 40.0 37.0 41.0 30.0 41.0 18-19 36.9105158289746 40.0 37.0 41.0 30.0 41.0 20-21 36.8883275659596 40.0 37.0 41.0 30.0 41.0 22-23 36.82863572205053 40.0 37.0 41.0 30.0 41.0 24-25 36.761132786191695 40.0 37.0 41.0 30.0 41.0 26-27 36.633242696398995 40.0 36.5 41.0 30.0 41.0 28-29 36.446309829388184 40.0 36.0 41.0 29.0 41.0 30-31 36.40347285065727 40.0 36.0 41.0 29.5 41.0 32-33 36.28872938495891 40.0 36.0 41.0 29.0 41.0 34-35 36.163966767405995 40.0 35.0 41.0 29.0 41.0 36-37 36.02912998028848 40.0 35.0 41.0 28.0 41.0 38-39 35.863408574794676 40.0 35.0 41.0 27.5 41.0 40-41 35.655362661567096 40.0 35.0 41.0 27.0 41.0 42-43 35.48915629393117 39.0 35.0 41.0 26.0 41.0 44-45 35.273388218241976 39.0 35.0 41.0 25.5 41.0 46-47 35.006254944479544 39.0 35.0 41.0 24.0 41.0 48-49 34.75398119532615 39.0 34.0 41.0 23.5 41.0 50-51 34.53272344304624 39.0 34.0 41.0 23.0 41.0 52-53 34.327828242298 38.0 34.0 41.0 22.5 41.0 54-55 34.104889129828514 38.0 34.0 40.0 22.0 41.0 56-57 33.83955057170989 37.5 33.0 40.0 21.5 41.0 58-59 33.68122786248608 37.0 33.0 40.0 21.5 41.0 60-61 33.55154044845125 37.0 33.0 40.0 21.5 41.0 62-63 33.278433247391874 36.0 33.0 40.0 20.5 41.0 64-65 32.97810081710786 36.0 33.0 39.0 20.0 41.0 66-67 32.67547282030267 35.0 33.0 39.0 20.0 41.0 68-69 32.35564467475996 35.0 32.5 38.5 19.0 40.5 70-71 32.05418106138649 35.0 32.0 37.5 18.0 40.0 72-73 31.746440923311006 35.0 32.0 37.0 18.0 39.0 74-75 31.464180625039127 35.0 32.0 36.5 17.0 39.0 76-77 31.18234007395139 35.0 32.0 36.0 14.0 39.0 78-79 30.914366120094176 35.0 32.0 35.5 12.5 37.0 80-81 30.69872989719277 35.0 31.0 35.0 11.0 37.0 82-83 30.519195489306647 35.0 31.0 35.0 10.0 36.5 84-85 30.33762993191084 35.0 31.0 35.0 7.5 36.0 86-87 30.20557343631131 35.0 31.0 35.0 7.0 36.0 88-89 30.10243396451927 35.0 31.0 35.0 7.0 35.5 90-91 30.008322140075087 35.0 31.0 35.0 7.0 35.0 92-93 30.07053863286682 35.0 31.0 35.0 7.0 35.0 94-95 30.041415055501485 35.0 31.0 35.0 3.5 35.0 96-97 30.028827857174022 35.0 31.0 35.0 2.0 35.0 98-99 29.985135732484924 35.0 31.0 35.0 2.0 35.0 100-101 29.93708108282442 35.0 31.0 35.0 2.0 35.0 102-103 29.05169103571788 33.5 29.5 35.0 2.0 35.0 104-105 29.760465696457807 34.5 31.0 35.0 2.0 35.0 106-107 29.870836202791487 35.0 31.0 35.0 2.0 35.0 108-109 29.876931548482936 35.0 31.0 35.0 2.0 35.0 110-111 29.84451852863672 35.0 31.0 35.0 2.0 35.0 112-113 29.79604816326221 35.0 31.0 35.0 2.0 35.0 114-115 29.728752966687725 35.0 31.0 35.0 2.0 35.0 116-117 29.651710623919804 35.0 31.0 35.0 2.0 35.0 118-119 29.555832778413325 35.0 31.0 35.0 2.0 35.0 120-121 29.43150769394217 35.0 31.0 35.0 2.0 35.0 122-123 29.313513203301824 35.0 31.0 35.0 2.0 35.0 124-125 28.390482742462098 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34593.0 3 12972.0 4 7492.0 5 6123.0 6 6662.0 7 7538.0 8 8766.0 9 9784.0 10 10997.0 11 11888.0 12 12637.0 13 14066.0 14 17417.0 15 14679.0 16 8373.0 17 7191.0 18 6622.0 19 6034.0 20 5808.0 21 6156.0 22 7234.0 23 9889.0 24 13800.0 25 19275.0 26 23922.0 27 26915.0 28 31600.0 29 37770.0 30 43906.0 31 52686.0 32 65692.0 33 85208.0 34 117129.0 35 188866.0 36 381261.0 37 602761.0 38 184261.0 39 439.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.96964292328249 17.428228994830796 12.362970539437608 25.2391575424491 2 17.089842869955667 19.50036544628319 37.13289960400895 26.27689207975219 3 19.34623414339339 23.75342321491172 28.89703339558726 28.003309246107627 4 13.632575775551558 15.302793741487559 35.02042985748929 36.044200625471596 5 14.596725299560989 36.88317097723915 33.25760299973718 15.262500723462677 6 34.16608159392789 34.46674573055029 16.46959203036053 14.897580645161291 7 29.926620884372753 31.28922680356132 20.136730210669075 18.647422101396856 8 28.33906858827352 32.94594472811097 19.021109374592385 19.693877309023126 9 27.850708168135206 14.119441623619105 18.572498478651557 39.45735172959413 10-11 26.699412500747062 24.461711991606343 26.58780376974735 22.25107173789925 12-13 27.046805261085893 22.184119033728436 26.842542130901055 23.926533574284615 14-15 24.418092566852746 23.193033023746334 24.44752321309614 27.94135119630478 16-17 24.389890460381363 26.23581304694508 24.94188134164569 24.43241515102787 18-19 24.42054880547629 25.111796870256814 26.873012605488434 23.59464171877846 20-21 25.376688424377587 24.115767371402725 26.735681641863223 23.771862562356464 22-23 25.16095890167295 23.781480577614005 26.626694642814368 24.43086587789868 24-25 24.592789994525944 24.310074445265606 26.606521621572902 24.490613938635548 26-27 24.428538022125636 24.313915966593825 26.71400646892445 24.54353954235609 28-29 24.526284082500048 24.369106122991102 26.765625296472688 24.338984498036165 30-31 24.743726159262884 24.27826104281833 26.561801025522307 24.416211772396476 32-33 24.433596589614062 24.41519181404915 26.461300045988224 24.689911550348565 34-35 24.66335325114469 24.444923045394773 26.56812441769339 24.323599285767145 36-37 24.80250250459175 24.27988243598112 26.422257092548463 24.49535796687867 38-39 24.835270398481974 24.506048387096772 26.241793168880456 24.416888045540798 40-41 24.880455173951297 24.41434775081171 26.29933995982364 24.405857115413347 42-43 24.754467912025625 24.4403889048976 26.2238064914721 24.581336691604676 44-45 24.761462240969387 24.51248830674006 25.98190177471903 24.744147677571522 46-47 24.869775422195172 24.669024884622992 25.714806158563512 24.74639353461832 48-49 25.021193204701923 24.811799123998394 25.351033948501577 24.815973722798102 50-51 24.897371456979027 25.06593652636892 25.21960658188842 24.817085434763634 52-53 25.10588335752711 24.936953861042323 25.28833290638431 24.668829875046253 54-55 24.929295181351574 24.887242298606303 25.33440699031337 24.849055529728755 56-57 24.848130927021014 24.97756742403344 25.23141191570048 24.942889733245067 58-59 24.880928069667366 24.75903958802215 25.408352503671004 24.95167983863948 60-61 24.884102477010842 24.95115641356553 25.542141869241743 24.622599240181888 62-63 24.816689101442744 24.9799747386398 25.42331754004163 24.78001861987583 64-65 24.866530285510986 24.734608200658187 25.486293693853952 24.912567819976875 66-67 24.824521271259343 24.90703392505745 25.449995173519675 24.818449630163535 68-69 24.95199108893888 24.831684173473466 25.288859940169132 24.927464797418526 70-71 24.73931576495679 24.66612210201954 25.61059548239035 24.98396665063332 72-73 25.05221038799938 24.957431189614134 25.25476653269657 24.735591889689914 74-75 24.77601135846674 25.136086708402296 25.22401236034954 24.863889572781424 76-77 24.84729350054005 24.84034421043494 25.234342869909764 25.078019419115243 78-79 24.79780395944859 24.90692280814036 25.233140722751067 25.062132509659985 80-81 24.726788593646155 25.064315034798668 25.108006089233164 25.100890282322013 82-83 24.900235223357836 24.967092921052895 25.117552386883844 25.01511946870543 84-85 24.766496003969348 25.04861479554748 25.002935031564338 25.181954168918836 86-87 24.5521961322003 25.027304752343227 25.215953612397985 25.20454550305849 88-89 24.6901480443979 24.937635867470437 25.153007674399575 25.219208413732087 90-91 24.589848998402157 24.933632773468194 25.172467034586855 25.304051193542794 92-93 24.55248195294746 25.05297074952128 25.178004011879107 25.21654328565215 94-95 24.557561056360775 24.853611300709545 25.271160015121495 25.317667627808184 96-97 24.508778594997928 24.85342921796822 25.27150159341329 25.366290593620562 98-99 24.518864212907552 24.849617275521545 25.153762111545387 25.47775640002552 100-101 24.492781843007073 24.934867128943043 25.240959012005792 25.33139201604409 102-103 24.47383654955068 24.77916988699465 25.33270661936589 25.414286944088783 104-105 24.310238150178254 24.913884263770633 25.239801940328345 25.536075645722768 106-107 24.4057306731594 24.903025196492994 25.123191260137684 25.56805287020992 108-109 24.331906647549314 24.85991084103305 25.209763132209773 25.598419379207865 110-111 24.399809618081257 24.888736376772172 25.191146719054103 25.520307286092468 112-113 24.351181898646153 24.849446309632118 25.265579745548134 25.53379204617359 114-115 24.067456237894913 25.096573900229064 25.27325154936812 25.562718312507897 116-117 24.084803964234798 25.015219965559123 25.247403000734497 25.652573069471586 118-119 24.15669685674685 24.76690993919926 25.239232747588225 25.837160456465664 120-121 23.920970626040518 24.849738892867816 25.359834561003257 25.869455920088413 122-123 23.922238590626627 24.883347451477377 25.29014920332878 25.904264754567212 124-125 23.855892491977198 25.03092535053277 25.259138426257245 25.85404373123279 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 22.0 1 19.5 2 47.0 3 94.5 4 82.5 5 66.0 6 120.5 7 263.5 8 457.0 9 546.5 10 467.5 11 388.5 12 420.0 13 530.0 14 654.5 15 791.5 16 928.5 17 1129.0 18 1357.0 19 1534.0 20 1745.5 21 2041.5 22 2333.5 23 2709.0 24 3231.5 25 3913.5 26 5257.0 27 6826.5 28 7884.5 29 9380.0 30 11669.0 31 13735.0 32 16007.0 33 18485.5 34 21273.5 35 24280.0 36 28079.5 37 32107.5 38 35031.0 39 38514.0 40 42116.0 41 45089.0 42 48488.5 43 54340.5 44 60077.0 45 64143.0 46 69559.0 47 74603.0 48 80609.0 49 86584.0 50 88050.5 51 88499.0 52 88586.5 53 88873.0 54 90459.5 55 91080.0 56 87611.5 57 80537.0 58 75787.5 59 70983.5 60 63580.5 61 55810.0 62 47752.0 63 38224.5 64 28798.5 65 22090.0 66 16961.5 67 13168.0 68 10734.5 69 8601.0 70 7079.5 71 5757.5 72 4613.0 73 4180.5 74 3446.5 75 2703.5 76 1940.0 77 1139.5 78 647.5 79 335.5 80 194.0 81 79.0 82 39.5 83 17.5 84 8.0 85 7.0 86 6.0 87 3.5 88 1.5 89 0.5 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1175292115582723 2 0.0014703008709872642 3 0.017975613874328167 4 0.05700973054602232 5 0.023429955815087375 6 0.01954077286602429 7 0.015129870253062495 8 0.005074909457923783 9 0.004031470130126369 10-11 0.007304075294581894 12-13 0.004837764156151644 14-15 0.003106603453215026 16-17 0.010671538579746275 18-19 0.005881203483949057 20-21 0.012829560825872744 22-23 0.008892948816455228 24-25 0.013564711261366375 26-27 0.00848980180344259 28-29 0.01384928562349294 30-31 0.019588201926378716 32-33 0.012497557403391748 34-35 0.019730489107442 36-37 0.013469853140657517 38-39 0.01954077286602429 40-41 0.009817815493366572 42-43 0.008750661635391944 44-45 0.017074461727594036 46-47 0.015319586494480205 48-49 0.02015735065063185 50-51 0.015367015554834634 52-53 0.020489354073112847 54-55 0.016695029244758613 56-57 0.020323352361872347 58-59 0.01600730786961941 60-61 0.019327342094429364 62-63 0.021319362629315334 64-65 0.016576456593872545 66-67 0.011880979618784185 68-69 0.022291658366581105 70-71 0.014513292468454933 72-73 0.016813601895644684 74-75 0.018070471995037023 76-77 0.01342242408030309 78-79 0.029334873829213643 80-81 0.02034706689204956 82-83 0.00962809925194886 84-85 0.012212983041265179 86-87 0.01245012834303732 88-89 0.020370781422226775 90-91 0.012118124920556323 92-93 0.008205227441316024 94-95 0.007351504354936322 96-97 0.0021343077159492545 98-99 0.001968306004708757 100-101 0.010505536868505775 102-103 0.002869458151442887 104-105 0.002727170970379603 106-107 1.6600171124049758E-4 108-109 0.017999328404505383 110-111 8.774376165569159E-4 112-113 0.009201237708759009 114-115 0.002490025668607464 116-117 0.007659793247240102 118-119 0.010149818915847566 120-121 0.0038891829490630865 122-123 6.640068449619903E-4 124-125 0.024354822491998718 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2108412.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.89001597121763 #Duplication Level Percentage of deduplicated Percentage of total 1 78.16822744945769 42.12487025693047 2 11.11549194909424 11.980280773292591 3 3.81102358848152 6.161283661498686 4 1.9024349954046238 4.1008900914623405 5 1.1193768830053896 3.0161619051486133 6 0.7189232717364129 2.3245671957573193 7 0.5195296527520515 1.9598222899030442 8 0.4112826387339489 1.7731222376045626 9 0.3001230823099982 1.455627392911518 >10 1.790093085392474 17.51921005304373 >50 0.10754172062817503 3.9155598234043443 >100 0.03515722498324891 3.1593156713967905 >500 5.296386801340675E-4 0.1840022874177542 >1k 2.6481934006702403E-4 0.32528636022817126 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2901 0.1375917040881953 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2606 0.12360013128363907 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.742906035442788E-5 2 0.0 0.0 0.0 4.742906035442788E-5 4.742906035442788E-5 3 0.0 0.0 0.0 4.742906035442788E-5 4.742906035442788E-5 4 0.0 0.0 0.0 9.485812070885576E-5 4.742906035442788E-5 5 0.0 0.0 0.0 9.485812070885576E-5 4.742906035442788E-5 6 0.0 0.0 0.0 9.485812070885576E-5 4.742906035442788E-5 7 0.0 0.0 0.0 9.485812070885576E-5 4.742906035442788E-5 8 0.0 0.0 0.0 9.485812070885576E-5 4.742906035442788E-5 9 0.0 0.0 0.0 9.485812070885576E-5 9.485812070885576E-5 10-11 0.0 0.0 0.0 9.485812070885576E-5 9.485812070885576E-5 12-13 0.0 0.0 0.0 9.485812070885576E-5 1.4228718106328364E-4 14-15 0.0 0.0 0.0 9.485812070885576E-5 1.4228718106328364E-4 16-17 0.0 0.0 0.0 9.485812070885576E-5 1.4228718106328364E-4 18-19 0.0 0.0 0.0 9.485812070885576E-5 2.1343077159492545E-4 20-21 0.0 0.0 0.0 9.485812070885576E-5 2.3714530177213942E-4 22-23 0.0 0.0 0.0 1.1857265088606971E-4 2.845743621265673E-4 24-25 0.0 0.0 0.0 1.8971624141771152E-4 3.3200342248099516E-4 26-27 0.0 0.0 0.0 4.7429060354427883E-4 3.3200342248099516E-4 28-29 0.0 0.0 0.0 0.0015414444615189063 3.3200342248099516E-4 30-31 0.0 0.0 0.0 0.004908907746683286 3.3200342248099516E-4 32-33 0.0 0.0 0.0 0.011999552269670255 4.2686154318985096E-4 34-35 0.0 0.0 0.0 0.021319362629315334 4.7429060354427883E-4 36-37 0.0 0.0 0.0 0.03395920721377037 4.7429060354427883E-4 38-39 0.0 0.0 0.0 0.055705431386275546 4.7429060354427883E-4 40-41 0.0 0.0 0.0 0.08112740773624888 4.7429060354427883E-4 42-43 0.0 0.0 0.0 0.10422536012885528 4.7429060354427883E-4 44-45 0.0 0.0 0.0 0.13208993308708167 4.7429060354427883E-4 46-47 0.0 0.0 0.0 0.16479227020145967 4.7429060354427883E-4 48-49 0.0 0.0 0.0 0.19972377315249581 4.7429060354427883E-4 50-51 0.0 0.0 0.0 0.2362915786857597 4.7429060354427883E-4 52-53 0.0 0.0 0.0 0.27442454321071974 5.454341940759207E-4 54-55 0.0 0.0 0.0 0.31528467870605936 6.165777846075625E-4 56-57 0.0 0.0 0.0 0.3643974707030694 6.402923147847764E-4 58-59 0.0 0.0 0.0 0.41744687470949704 6.640068449619903E-4 60-61 0.0 0.0 0.0 0.47725491981643053 6.640068449619903E-4 62-63 0.0 0.0 0.0 0.537157823044073 6.640068449619903E-4 64-65 0.0 0.0 0.0 0.5995981810006773 6.640068449619903E-4 66-67 0.0 0.0 0.0 0.671287205726395 6.640068449619903E-4 68-69 0.0 0.0 0.0 0.7522723262815807 6.640068449619903E-4 70-71 0.0 0.0 0.0 0.8403243768295761 6.640068449619903E-4 72-73 4.742906035442788E-5 2.371453017721394E-5 0.0 0.9348979231763053 7.588649656708462E-4 74-75 4.742906035442788E-5 4.742906035442788E-5 0.0 1.0339535157265278 8.30008556202488E-4 76-77 4.742906035442788E-5 4.742906035442788E-5 0.0 1.145482951149965 8.537230863797019E-4 78-79 4.742906035442788E-5 4.742906035442788E-5 0.0 1.2722134004169963 8.537230863797019E-4 80-81 4.742906035442788E-5 4.742906035442788E-5 0.0 1.4094731010827106 9.011521467341297E-4 82-83 4.742906035442788E-5 4.742906035442788E-5 0.0 1.556811477073741 9.485812070885577E-4 84-85 4.742906035442788E-5 4.742906035442788E-5 0.0 1.704576714607961 9.485812070885577E-4 86-87 4.742906035442788E-5 4.742906035442788E-5 0.0 1.8691081249774713 9.485812070885577E-4 88-89 4.742906035442788E-5 4.742906035442788E-5 0.0 2.058658364683942 9.960102674429856E-4 90-91 4.742906035442788E-5 4.742906035442788E-5 0.0 2.2674410883641336 9.960102674429856E-4 92-93 4.742906035442788E-5 4.742906035442788E-5 0.0 2.491614542129337 9.960102674429856E-4 94-95 4.742906035442788E-5 4.742906035442788E-5 0.0 2.7295187088671478 0.0010908683881518412 96-97 4.742906035442788E-5 4.742906035442788E-5 0.0 2.9784975611977167 0.0010908683881518412 98-99 4.742906035442788E-5 4.742906035442788E-5 0.0 3.2475626205883863 0.0010908683881518412 100-101 4.742906035442788E-5 4.742906035442788E-5 0.0 3.548618581188117 0.0011382974485062692 102-103 4.742906035442788E-5 9.485812070885576E-5 0.0 3.87808454894015 0.001185726508860697 104-105 4.742906035442788E-5 9.485812070885576E-5 0.0 4.214996879167829 0.001233155569215125 106-107 4.742906035442788E-5 9.485812070885576E-5 0.0 4.562201315492418 0.001233155569215125 108-109 7.114359053164182E-5 9.485812070885576E-5 0.0 4.933357427295993 0.0013042991597467667 110-111 9.485812070885576E-5 9.485812070885576E-5 0.0 5.33837788819263 0.0013991572804556225 112-113 9.485812070885576E-5 9.485812070885576E-5 0.0 5.772685793858127 0.0014228718106328365 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2915 0.0 75.85705 1 GTATCAA 5785 0.0 60.68377 1 TCAACGC 7650 0.0 45.21906 4 CAACGCA 7770 0.0 44.496403 5 AACGCAG 8100 0.0 42.821373 6 TATCAAC 8385 0.0 41.682346 2 ATCAACG 8340 0.0 41.620693 3 ACGCAGA 9745 0.0 35.22412 7 CGCAGAG 10400 0.0 33.062096 8 GTACATG 10310 0.0 32.836002 1 TACATGG 10865 0.0 30.743282 2 GCAGAGT 11285 0.0 29.888702 9 ACATGGG 11165 0.0 29.59655 3 GTGGTAT 1105 0.0 26.969217 1 CATGGGG 9350 0.0 26.936153 4 AGAGTAC 10245 0.0 26.797262 10-11 CAGAGTA 10845 0.0 25.616394 10-11 GTATAGG 685 0.0 24.36285 1 GAGTACT 5795 0.0 22.892187 12-13 ATGGGGG 4930 0.0 22.088892 5 >>END_MODULE