FastQCFastQC Report
Mon 15 Aug 2016
SRR2921480_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921480_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1989495
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG20160.10133224763068013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20400.073.2206951
GTATCAA40650.059.2904551
TCAACGC52500.044.8740354
CAACGCA53900.043.7084735
ATCAACG57050.041.2930453
AACGCAG57900.040.585126
GTACATG101200.039.575361
TATCAAC62850.038.051262
TACATGG104550.037.6119422
ACATGGG105550.036.634693
ACGCAGA68950.033.9083447
CATGGGG84400.032.2836234
CGCAGAG74700.031.1374198
GCAGAGT80050.028.0903369
ATGGGGG43100.026.226215
CATGGGT20000.025.58164
AGAGTAC72800.025.53618810-11
ACACCGT8700.024.6167986
ATGGGGA35400.024.2001725
CAGAGTA77250.023.91115210-11
Produced by FastQC (version 0.11.5)