FastQCFastQC Report
Mon 15 Aug 2016
SRR2921480_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921480_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1989495
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29150.14651959416836935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26300.13219435082772263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28400.076.178741
GTATCAA57700.059.186721
TCAACGC75000.045.6484154
CAACGCA76850.044.6786275
ATCAACG79850.042.950353
AACGCAG80100.042.934726
TATCAAC84150.040.5433462
ACGCAGA93750.036.681587
GTACATG104850.034.219491
CGCAGAG100850.034.0358668
TACATGG109250.032.5910872
ACATGGG108600.032.073413
GCAGAGT110550.030.6712289
CATGGGG84300.029.0290854
TACCGTA1704.4296576E-727.9983547
GTATAGG5350.026.73641
CCGTATG2455.5661076E-1026.7080179
AGAGTAC100500.026.45963110-11
CAGAGTA105150.026.33617810-11
GTGGTAT11500.025.9129961
Produced by FastQC (version 0.11.5)