FastQCFastQC Report
Mon 15 Aug 2016
SRR2921479_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921479_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1709920
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20100.068.100571
GTATCAA36400.055.5899771
TCAACGC46600.043.1503684
CAACGCA47850.042.270565
ATCAACG51200.039.3886223
AACGCAG50650.039.3442236
TATCAAC51300.038.9639432
GTACATG84100.033.826021
ACGCAGA59300.033.402567
TACATGG88150.031.8537622
CGCAGAG62950.031.4639558
ACATGGG90150.030.751143
GTATACG1055.898728E-428.3399871
GCAGAGT68300.028.0414169
CATGGGG77050.027.1011814
CAGAGTA64800.025.28855910-11
ATGGGGG37650.024.9650925
AGAGTAC60600.024.8818610-11
GTGGTAT9650.020.9686551
GTATAGA5450.020.7479921
Produced by FastQC (version 0.11.5)