##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921479_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1709920
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.837738022831477	31.0	30.0	34.0	26.0	34.0
2	30.0056599139141	31.0	30.0	34.0	25.0	34.0
3	30.09187564330495	31.0	30.0	34.0	26.0	34.0
4	33.48803979133527	37.0	35.0	37.0	27.0	37.0
5	33.51088939833443	37.0	35.0	37.0	27.0	37.0
6	33.51332986338542	37.0	35.0	37.0	27.0	37.0
7	33.35052751005895	37.0	35.0	37.0	26.0	37.0
8	33.29222946102742	37.0	35.0	37.0	26.0	37.0
9	34.7740765649855	39.0	35.0	39.0	27.0	39.0
10-11	34.662430113689524	39.0	35.0	39.0	26.5	39.0
12-13	34.51102215308319	39.0	35.0	39.0	25.5	39.0
14-15	35.67000181295032	40.0	36.0	41.0	25.0	41.0
16-17	35.594933388696546	40.0	36.0	41.0	25.0	41.0
18-19	35.60910773603443	40.0	36.0	41.0	25.0	41.0
20-21	35.62624362543277	40.0	36.0	41.0	25.0	41.0
22-23	35.59329325348554	40.0	36.0	41.0	25.0	41.0
24-25	35.55599150837466	40.0	36.0	41.0	25.0	41.0
26-27	35.48244186862543	40.0	35.0	41.0	25.0	41.0
28-29	35.42972478244596	40.0	35.0	41.0	25.0	41.0
30-31	35.329525942734165	40.0	35.0	41.0	25.0	41.0
32-33	35.22524182417891	40.0	35.0	41.0	24.5	41.0
34-35	35.070686640310655	40.0	35.0	41.0	24.0	41.0
36-37	34.92807353560401	39.5	35.0	41.0	24.0	41.0
38-39	34.800835419200894	39.0	35.0	41.0	23.0	41.0
40-41	34.6545168779826	39.0	34.0	41.0	22.5	41.0
42-43	34.46151749789463	39.0	34.0	41.0	21.0	41.0
44-45	34.25917323617479	39.0	33.5	41.0	19.5	41.0
46-47	33.99460676522878	38.5	33.0	41.0	18.0	41.0
48-49	33.84637526901843	38.0	33.0	40.5	16.0	41.0
50-51	33.65077313558529	38.0	33.0	40.0	15.0	41.0
52-53	33.44951196547207	38.0	33.0	40.0	13.5	41.0
54-55	33.23327202442219	38.0	33.0	40.0	12.5	41.0
56-57	32.98472530878637	37.0	32.5	40.0	9.0	41.0
58-59	32.731188886029756	37.0	32.0	40.0	8.0	41.0
60-61	32.42116151632825	36.0	32.0	40.0	7.5	41.0
62-63	32.103137866099	36.0	31.5	39.0	6.5	41.0
64-65	31.786094963507065	35.0	31.0	39.0	3.5	41.0
66-67	31.746581419013754	35.0	32.0	39.0	2.0	41.0
68-69	31.496843711986525	35.0	31.0	38.0	2.0	40.0
70-71	31.198306353513615	35.0	31.0	37.0	2.0	40.0
72-73	30.87208407410873	35.0	31.0	37.0	2.0	39.0
74-75	30.576710021521475	35.0	31.0	36.0	2.0	39.0
76-77	30.307257649480675	35.0	31.0	36.0	2.0	38.5
78-79	30.039624368391504	35.0	30.5	35.0	2.0	37.0
80-81	29.812240338729296	35.0	30.0	35.0	2.0	37.0
82-83	29.62181242397305	35.0	30.0	35.0	2.0	36.0
84-85	29.42754807242444	35.0	30.0	35.0	2.0	36.0
86-87	29.278522094600916	34.5	30.0	35.0	2.0	36.0
88-89	29.165000116964535	34.0	30.0	35.0	2.0	35.5
90-91	29.05887322213905	34.0	30.0	35.0	2.0	35.0
92-93	28.958337524562552	34.0	30.0	35.0	2.0	35.0
94-95	28.894541265088424	34.0	30.0	35.0	2.0	35.0
96-97	28.79390322354262	34.0	29.0	35.0	2.0	35.0
98-99	28.735914838121083	34.0	29.0	35.0	2.0	35.0
100-101	28.671191634696363	34.0	29.0	35.0	2.0	35.0
102-103	27.698314248619816	32.5	28.0	34.5	2.0	35.0
104-105	28.522251625807055	34.0	29.0	35.0	2.0	35.0
106-107	28.84845372882942	34.5	30.0	35.0	2.0	35.0
108-109	28.88751988397118	35.0	30.0	35.0	2.0	35.0
110-111	28.828141375035088	35.0	30.0	35.0	2.0	35.0
112-113	28.760568330682137	35.0	30.0	35.0	2.0	35.0
114-115	28.642765743426594	35.0	30.0	35.0	2.0	35.0
116-117	28.577589010012165	35.0	30.0	35.0	2.0	35.0
118-119	28.4265921797511	34.5	30.0	35.0	2.0	35.0
120-121	28.254343770468793	34.0	29.5	35.0	2.0	35.0
122-123	28.13635608683447	34.0	29.5	35.0	2.0	35.0
124-125	27.283732279872744	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	64964.0
3	11506.0
4	7738.0
5	6616.0
6	7155.0
7	7923.0
8	8723.0
9	9073.0
10	9472.0
11	9910.0
12	10356.0
13	10496.0
14	11388.0
15	10012.0
16	7379.0
17	6569.0
18	5813.0
19	5591.0
20	5402.0
21	5762.0
22	6681.0
23	9010.0
24	12989.0
25	17590.0
26	21141.0
27	22298.0
28	26598.0
29	31818.0
30	36084.0
31	43573.0
32	55013.0
33	72546.0
34	101403.0
35	162467.0
36	312131.0
37	442792.0
38	113827.0
39	111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.02283397541092	16.796515522481492	12.144539122854745	25.03611137925285
2	17.001409165012696	18.93206467535096	37.902151652829474	26.16437450680687
3	19.7327181068683	23.26479111509407	28.25670105866841	28.74578971936922
4	13.654154954912794	14.690853571146528	34.9109809209314	36.744010553009275
5	14.80030045313668	36.58245086924322	33.06230541622938	15.554943261390719
6	34.43565094426278	33.72438958891703	16.862107034975665	14.977852431844523
7	30.493814232151017	30.78451343715977	19.937671375927486	18.78400095476173
8	28.248363153042266	33.06237802623635	19.012813972752756	19.676444847968625
9	27.69692995361674	14.710027125636097	18.491880151136066	39.1011627696111
10-11	26.74749507497591	24.560213995404773	26.363877014067942	22.328413915551376
12-13	26.924177187050276	22.123108963483837	26.613801495278988	24.3389123541869
14-15	24.298841261785917	23.338330206022277	23.845115471634095	28.51771306055771
16-17	24.649922705554232	26.392199476708555	24.3604012583565	24.597476559380706
18-19	24.71745664908983	25.536015446161496	25.885163055005012	23.86136484974366
20-21	25.67435513638071	24.44592227112769	25.67286300605161	24.20685958643999
22-23	25.526167125441358	24.113829562838283	25.526254891881983	24.833748419838376
24-25	24.929414277480156	24.685575350441656	25.518355213688217	24.86665515838997
26-27	24.662695353809692	24.69294387436742	25.69272476274365	24.951636009079238
28-29	24.776799231204667	24.63787194643006	25.704502338126602	24.88082648423867
30-31	24.95727455857203	24.6993714489322	25.500023695725815	24.84333029676995
32-33	24.64903593678917	24.734346863814093	25.539154234022853	25.077462965373886
34-35	24.949716434623177	24.76045833806235	25.56771578640688	24.722109440907598
36-37	25.06471975446719	24.552022863502096	25.442228495181237	24.941028886849477
38-39	25.034253398788557	24.79235111291728	25.401992855865966	24.771402632428188
40-41	24.94892257645524	24.727967375977812	25.5464933271705	24.77661672039645
42-43	24.921189268926057	24.666917690730745	25.500855910139236	24.911037130203958
44-45	24.878824308339656	24.77443489134343	25.399162251522906	24.947578548794006
46-47	24.960200971561505	24.83494484897728	25.30161972756135	24.903234451899863
48-49	25.06110498882923	24.893068097944678	25.031119297123684	25.014707616102406
50-51	24.988722167450213	25.10849245657815	24.94598049856504	24.956804877406597
52-53	25.0926154028002	24.965987867396862	25.15136496173964	24.790031768063294
54-55	25.018846784491373	24.932957519040592	25.145749929425122	24.90244576704292
56-57	24.878570768289272	24.962292874084525	25.098436368274296	25.060699989351903
58-59	25.052861049451263	24.836941068267105	25.065966611241063	25.04423127104057
60-61	25.01235820242414	24.979861248830723	25.17615922863757	24.83162132010757
62-63	24.8540877088494	25.068158708576764	25.13599563784265	24.941757944731187
64-65	24.92185966537069	24.782208955957373	25.23126175030254	25.064669628369394
66-67	24.886945594651547	25.05221270725666	25.125544503947104	24.935297194144688
68-69	24.97204483239623	25.01916570606578	25.061226423841582	24.94756303769641
70-71	24.839181615874807	24.77297905945563	25.28969834979231	25.09814097487726
72-73	25.042835840889932	24.966494738313816	25.05567635584431	24.934993064951946
74-75	24.840563295245595	25.152884311053832	25.055651468818503	24.95090092488207
76-77	24.916228602316348	24.921961561096374	25.083303401048546	25.078506435538728
78-79	24.796749988811925	24.882481348091257	25.218299067602	25.10246959549482
80-81	24.827051884288476	25.14769285034698	24.922311177041603	25.102944088322943
82-83	24.965891912237588	25.09413539762938	24.906464144320566	25.03350854581246
84-85	24.811136200988344	25.106872445904276	24.89077324557953	25.191218107527853
86-87	24.66092395735725	25.16854366342506	25.098148973530193	25.072383405687493
88-89	24.82832686436338	24.99322198574321	25.037589828796282	25.14086132109713
90-91	24.747056609545247	25.01008205727193	25.03502765510503	25.20783367807779
92-93	24.80373556806061	25.2104927441457	25.012553963030953	24.973217724762733
94-95	24.746358713644163	24.84234057710777	25.171258315796123	25.240042393451944
96-97	24.78492406762412	25.001067410637255	25.055110557422307	25.158897964316317
98-99	24.740569782890137	25.02993847369494	25.00581223115689	25.22367951225803
100-101	24.687979421468604	25.007559615684933	25.089121581430657	25.215339381415802
102-103	24.76449261588507	24.846460356330113	25.230185183343966	25.158861844440843
104-105	24.51477951860888	25.081887490199968	25.148327407255344	25.25500558393581
106-107	24.55792558106891	25.049538537121734	25.096064990817535	25.296470890991824
108-109	24.634295988339584	25.01520720400347	25.059629786467458	25.290867021189484
110-111	24.64449266292746	25.060094396453408	25.11723524836093	25.178177692258203
112-113	24.631534559134025	25.058925227249603	25.159492897974843	25.150047315641533
114-115	24.475258422944517	25.24640395275688	25.139198055438257	25.13913956886034
116-117	24.414114817704466	25.327405565309746	25.053134683116095	25.20534493386969
118-119	24.624871332537683	24.95776541840666	25.110700912337304	25.306662336718354
120-121	24.318769928842276	25.083955314206342	25.248946395650552	25.34832836130083
122-123	24.39160785164781	25.22476252927346	25.13456211412926	25.24906750494947
124-125	24.421908651083633	25.22369357949204	25.161349749762046	25.193048019662285
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	92.0
1	115.0
2	153.5
3	152.0
4	124.5
5	121.0
6	142.0
7	175.0
8	220.5
9	270.0
10	308.0
11	344.0
12	400.5
13	480.5
14	545.5
15	590.0
16	657.0
17	748.5
18	839.0
19	910.0
20	1005.0
21	1156.5
22	1370.0
23	1644.0
24	1966.5
25	2505.5
26	3411.5
27	4340.0
28	5057.5
29	6254.5
30	7974.0
31	9602.5
32	11803.0
33	14335.5
34	16890.5
35	19483.5
36	22672.0
37	26154.0
38	28568.0
39	31201.5
40	33976.5
41	36153.0
42	38886.0
43	44117.0
44	49378.5
45	53141.5
46	57100.0
47	60909.0
48	65611.0
49	69936.0
50	71041.0
51	71779.0
52	72380.0
53	72711.0
54	74098.5
55	73961.0
56	70352.0
57	65188.5
58	61968.5
59	58148.5
60	52033.0
61	45693.5
62	39299.0
63	31366.0
64	24115.5
65	19090.5
66	14803.0
67	11566.0
68	9463.5
69	7776.5
70	6390.0
71	5114.5
72	4092.0
73	3599.0
74	2947.5
75	2369.5
76	1771.5
77	1074.5
78	647.5
79	395.0
80	244.5
81	123.0
82	66.0
83	46.5
84	36.0
85	24.0
86	20.0
87	17.5
88	16.5
89	15.5
90	11.5
91	8.0
92	7.0
93	9.0
94	10.0
95	6.5
96	7.5
97	8.0
98	6.0
99	6.5
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.036551417610180596
2	0.023217460465986713
3	0.024211659024983623
4	0.026609432020211474
5	0.02935809862449705
6	0.041113034527931136
7	0.03467998502853935
8	0.031346495742490876
9	0.027311219238326943
10-11	0.031434219144755304
12-13	0.03158042481519603
14-15	0.03026457378122953
16-17	0.0320190418265182
18-19	0.044300318143538873
20-21	0.05579208384017966
22-23	0.04903738186581828
24-25	0.0587746795171704
26-27	0.04359853092542341
28-29	0.04400790680265743
30-31	0.043920183400393
32-33	0.05131819032469355
34-35	0.03590811266024141
36-37	0.04798470103864508
38-39	0.056464629924206984
40-41	0.04386170113221671
42-43	0.05383292785627398
44-45	0.05465167961074203
46-47	0.05973963694207916
48-49	0.045119069898006924
50-51	0.047663048563675495
52-53	0.05678628239917657
54-55	0.043423084120894545
56-57	0.04058669411434453
58-59	0.04222419762328062
60-61	0.030937119865256855
62-63	0.03941704875081875
64-65	0.03833512678955741
66-67	0.03310096378777955
68-69	0.028451623467764576
70-71	0.045119069898006924
72-73	0.02848086460185272
74-75	0.036317488537475434
76-77	0.029592027697202206
78-79	0.02985519790399551
80-81	0.0219600917001965
82-83	0.01646275849162534
84-85	0.016872134368859364
86-87	0.01602414148030317
88-89	0.020381070459436698
90-91	0.011667212501169645
92-93	0.01757392158697483
94-95	0.012983063535136147
96-97	0.010234396930850567
98-99	0.009561850846823244
100-101	0.010351361467203144
102-103	0.005906709085805184
104-105	0.006111397024422195
106-107	0.008187517544680454
108-109	0.011930382707962945
110-111	0.006433049499391785
112-113	0.007368765790212407
114-115	0.007368765790212407
116-117	0.00666697857209694
118-119	0.2322915691962197
120-121	0.3443728361560775
122-123	0.0230127725273697
124-125	0.002777907738373725
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1709920.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.645010778515434
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.84956227410503	46.02928877950524
2	10.446478362453467	12.04374715602317
3	3.481806711111446	6.0212635617218
4	1.7576986690943712	4.052902349013091
5	1.0318492084617528	2.9740479371790185
6	0.6808763980395477	2.3549476382295897
7	0.49700759987203635	2.005500591613934
8	0.3656668264477396	1.6863094521540385
9	0.27305085453989886	1.4166017507731787
>10	1.5105910114813732	15.650651985193262
>50	0.08021858964608482	3.1434831501880636
>100	0.0245815917231978	2.3198465267666246
>500	4.079353494447988E-4	0.15702433467872248
>1k	2.039676747223994E-4	0.14438478696029292
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	5.848226817628895E-5
9	0.0	0.0	0.0	0.0	5.848226817628895E-5
10-11	0.0	0.0	0.0	0.0	5.848226817628895E-5
12-13	0.0	0.0	0.0	0.0	1.4620567044072236E-4
14-15	0.0	0.0	0.0	0.0	1.7544680452886684E-4
16-17	0.0	0.0	0.0	0.0	1.7544680452886684E-4
18-19	0.0	0.0	0.0	0.0	2.0468793861701132E-4
20-21	0.0	0.0	0.0	0.0	2.339290727051558E-4
22-23	0.0	0.0	0.0	0.0	2.339290727051558E-4
24-25	0.0	0.0	0.0	0.0	2.339290727051558E-4
26-27	0.0	0.0	0.0	1.4620567044072236E-4	2.339290727051558E-4
28-29	0.0	0.0	0.0	9.064751567324787E-4	2.339290727051558E-4
30-31	0.0	0.0	0.0	0.0027194254701974362	2.631702067933003E-4
32-33	0.0	0.0	0.0	0.007456489192476841	2.924113408814447E-4
34-35	0.0	0.0	0.0	0.014269673435014504	3.508936090577337E-4
36-37	0.0	0.0	0.0	0.02424090015907177	3.508936090577337E-4
38-39	0.0	0.0	0.0	0.038247403387292975	3.508936090577337E-4
40-41	0.0	0.0	0.0	0.053774445588097686	3.508936090577337E-4
42-43	0.0	0.0	0.0	0.0709974735660148	3.8013474314587813E-4
44-45	0.0	0.0	0.0	0.09377631702067932	4.0937587723402264E-4
46-47	0.0	0.0	0.0	0.11863128099560213	4.0937587723402264E-4
48-49	0.0	0.0	0.0	0.14345700383643678	4.0937587723402264E-4
50-51	0.0	0.0	0.0	0.1719378684382895	4.0937587723402264E-4
52-53	0.0	0.0	0.0	0.19962922241976233	4.678581454103116E-4
54-55	0.0	0.0	0.0	0.2311804061008702	5.263404135866005E-4
56-57	0.0	0.0	0.0	0.2703342846448957	5.263404135866005E-4
58-59	0.0	0.0	0.0	0.31361116309534953	5.263404135866005E-4
60-61	0.0	0.0	0.0	0.3572681762889492	5.848226817628894E-4
62-63	0.0	0.0	0.0	0.4010713951529896	7.017872181154674E-4
64-65	0.0	0.0	0.0	0.4459565359782914	7.017872181154674E-4
66-67	0.0	0.0	0.0	0.4999649106390942	7.017872181154674E-4
68-69	0.0	0.0	0.0	0.5655527743988022	7.017872181154674E-4
70-71	0.0	0.0	0.0	0.6384216805464583	7.017872181154674E-4
72-73	0.0	0.0	0.0	0.715881444745953	8.187517544680453E-4
74-75	0.0	0.0	0.0	0.7935166557499767	8.187517544680453E-4
76-77	0.0	0.0	0.0	0.8771462992420698	8.479928885561898E-4
78-79	0.0	0.0	0.0	0.9735835594647703	8.772340226443343E-4
80-81	0.0	5.848226817628895E-5	0.0	1.0848168335360717	9.357162908206232E-4
82-83	0.0	5.848226817628895E-5	0.0	1.2009333770000936	0.001052680827173201
84-85	0.0	5.848226817628895E-5	0.0	1.3191845232525499	0.00111116309534949
86-87	0.0	5.848226817628895E-5	0.0	1.4510327968559933	0.001169645363525779
88-89	0.0	5.848226817628895E-5	0.0	1.5935248432675213	0.001169645363525779
90-91	0.0	5.848226817628895E-5	0.0	1.764088378403668	0.001169645363525779
92-93	0.0	5.848226817628895E-5	0.0	1.9414358566482641	0.001169645363525779
94-95	0.0	5.848226817628895E-5	0.0	2.125713483671751	0.001169645363525779
96-97	0.0	5.848226817628895E-5	0.0	2.3180909048376535	0.0011988864976139233
98-99	0.0	5.848226817628895E-5	0.0	2.5265801908861234	0.001228127631702068
100-101	0.0	5.848226817628895E-5	0.0	2.772176476092449	0.001228127631702068
102-103	0.0	5.848226817628895E-5	0.0	3.0463413493028915	0.0013743333021427903
104-105	0.0	5.848226817628895E-5	0.0	3.3172019743613737	0.0014620567044072237
106-107	0.0	5.848226817628895E-5	0.0	3.603151024609338	0.0014620567044072237
108-109	0.0	5.848226817628895E-5	0.0	3.9039838121081685	0.0014620567044072237
110-111	0.0	5.848226817628895E-5	0.0	4.235607513801815	0.0015497801066716571
112-113	0.0	5.848226817628895E-5	0.0	4.592787966688499	0.0016375035089360905
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2010	0.0	68.10057	1
GTATCAA	3640	0.0	55.589977	1
TCAACGC	4660	0.0	43.150368	4
CAACGCA	4785	0.0	42.27056	5
ATCAACG	5120	0.0	39.388622	3
AACGCAG	5065	0.0	39.344223	6
TATCAAC	5130	0.0	38.963943	2
GTACATG	8410	0.0	33.82602	1
ACGCAGA	5930	0.0	33.40256	7
TACATGG	8815	0.0	31.853762	2
CGCAGAG	6295	0.0	31.463955	8
ACATGGG	9015	0.0	30.75114	3
GTATACG	105	5.898728E-4	28.339987	1
GCAGAGT	6830	0.0	28.041416	9
CATGGGG	7705	0.0	27.101181	4
CAGAGTA	6480	0.0	25.288559	10-11
ATGGGGG	3765	0.0	24.965092	5
AGAGTAC	6060	0.0	24.88186	10-11
GTGGTAT	965	0.0	20.968655	1
GTATAGA	545	0.0	20.747992	1
>>END_MODULE