FastQCFastQC Report
Mon 15 Aug 2016
SRR2921479_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921479_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1709920
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27390.1601829325348554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26270.15363291849911107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27200.079.578931
GTATCAA50950.057.698321
TCAACGC66550.043.844034
CAACGCA68200.042.7525
ATCAACG70600.041.32893
TATCAAC71300.041.1736952
AACGCAG70800.041.0067256
ACGCAGA85250.034.1248137
GTGGTAT9400.033.620831
CGCAGAG89950.032.4050458
GTACATG85700.031.7977051
GCAGAGT94000.030.1861869
TACATGG88800.029.9748482
ACATGGG89600.029.6407573
TGGTATC11550.026.8091962
CAGAGTA93250.026.5384110-11
CATGGGG78250.026.1019064
GAGTACT49650.025.88079312-13
AGAGTAC89250.024.99501610-11
GTACTTT53400.024.1197314-15
Produced by FastQC (version 0.11.5)