FastQCFastQC Report
Mon 15 Aug 2016
SRR2921478_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921478_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1619152
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18000.069.76031
GTATCAA37350.059.431491
TCAACGC45000.048.1215554
CAACGCA46700.046.369815
AACGCAG49600.043.658676
ATCAACG49550.043.5813143
TATCAAC52750.041.7269482
ACGCAGA57850.037.3285067
CGCAGAG62450.034.6741878
GTACATG82350.034.543211
TACATGG84950.033.1234172
ACATGGG85100.032.64563
GCAGAGT67250.031.3137369
CATGGGG71300.030.2043724
ATGGGGG38700.027.209035
CAGAGTA63650.026.44957510-11
AGAGTAC60100.026.03226310-11
ATGGGGA28800.022.722235
TATACCG2204.1846943E-621.6330665
GTGGTAT8750.020.4038261
Produced by FastQC (version 0.11.5)