##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921478_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1619152
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.586675000247045	31.0	30.0	34.0	25.0	34.0
2	29.730948669426958	31.0	30.0	34.0	25.0	34.0
3	29.82375095111515	31.0	30.0	34.0	25.0	34.0
4	33.198904117711	37.0	35.0	37.0	26.0	37.0
5	33.200845874877714	37.0	35.0	37.0	26.0	37.0
6	33.22276722630117	37.0	35.0	37.0	26.0	37.0
7	33.06918003992213	37.0	35.0	37.0	26.0	37.0
8	33.00609578347184	37.0	35.0	37.0	25.0	37.0
9	34.43743823927587	39.0	35.0	39.0	25.0	39.0
10-11	34.31576621589573	39.0	35.0	39.0	24.5	39.0
12-13	34.151298642746326	39.0	35.0	39.0	22.5	39.0
14-15	35.27939254622173	40.0	36.0	41.0	23.0	41.0
16-17	35.19877812583377	40.0	35.0	41.0	22.0	41.0
18-19	35.214788049546925	40.0	35.0	41.0	22.0	41.0
20-21	35.23085016107197	40.0	35.0	41.0	22.5	41.0
22-23	35.19703060614445	40.0	35.0	41.0	23.0	41.0
24-25	35.16147001640365	40.0	35.0	41.0	23.0	41.0
26-27	35.08292365386326	40.0	35.0	41.0	23.0	41.0
28-29	35.037486906726485	40.0	35.0	41.0	23.5	41.0
30-31	34.93557430062155	40.0	35.0	41.0	23.0	41.0
32-33	34.81742202090972	40.0	34.5	41.0	23.0	41.0
34-35	34.65426470152278	39.0	34.0	41.0	21.5	41.0
36-37	34.511399176853075	39.0	34.0	41.0	20.0	41.0
38-39	34.37867754231845	39.0	34.0	41.0	18.5	41.0
40-41	34.22286419063806	39.0	33.5	41.0	17.5	41.0
42-43	34.02842475567458	39.0	33.0	41.0	15.5	41.0
44-45	33.822681564176804	39.0	33.0	41.0	15.0	41.0
46-47	33.5561090002668	38.0	33.0	41.0	10.5	41.0
48-49	33.39873680790932	38.0	33.0	40.0	8.5	41.0
50-51	33.20664767730268	38.0	33.0	40.0	8.0	41.0
52-53	33.0061609410358	38.0	32.5	40.0	7.0	41.0
54-55	32.79440843107997	37.5	32.0	40.0	4.0	41.0
56-57	32.55954814619011	37.0	32.0	40.0	2.0	41.0
58-59	32.30849450823641	36.5	32.0	40.0	2.0	41.0
60-61	32.002755454707156	36.0	31.0	40.0	2.0	41.0
62-63	31.693472879630818	35.5	31.0	39.0	2.0	41.0
64-65	31.384149233672936	35.0	31.0	39.0	2.0	41.0
66-67	31.347180190618296	35.0	31.0	39.0	2.0	40.5
68-69	31.097412719744653	35.0	31.0	38.0	2.0	40.0
70-71	30.80636468966471	35.0	31.0	37.0	2.0	40.0
72-73	30.481091336699706	35.0	31.0	37.0	2.0	39.0
74-75	30.18951617883929	35.0	30.0	36.0	2.0	39.0
76-77	29.920303035169027	35.0	30.0	36.0	2.0	38.5
78-79	29.652755887032225	35.0	30.0	35.0	2.0	37.0
80-81	29.41875129697521	35.0	30.0	35.0	2.0	37.0
82-83	29.227631192130204	35.0	30.0	35.0	2.0	36.0
84-85	29.030635171991264	34.0	30.0	35.0	2.0	36.0
86-87	28.88346029279524	34.0	29.0	35.0	2.0	36.0
88-89	28.764021228396096	34.0	29.0	35.0	2.0	35.5
90-91	28.65751238920126	34.0	29.0	35.0	2.0	35.0
92-93	28.55567636639426	34.0	29.0	35.0	2.0	35.0
94-95	28.48708120052966	34.0	29.0	35.0	2.0	35.0
96-97	28.38823223514531	34.0	29.0	35.0	2.0	35.0
98-99	28.332422774390544	34.0	29.0	35.0	2.0	35.0
100-101	28.271081714378887	34.0	29.0	35.0	2.0	35.0
102-103	27.303289932013794	32.5	26.0	34.5	2.0	35.0
104-105	28.119173184481753	34.0	29.0	35.0	2.0	35.0
106-107	28.441682436238228	34.0	29.0	35.0	2.0	35.0
108-109	28.48339809974604	35.0	30.0	35.0	2.0	35.0
110-111	28.427252969455616	35.0	29.0	35.0	2.0	35.0
112-113	28.354883296935682	34.0	29.0	35.0	2.0	35.0
114-115	28.237446824016523	34.0	29.0	35.0	2.0	35.0
116-117	28.177496615512318	34.0	29.0	35.0	2.0	35.0
118-119	28.019628793343678	34.0	29.0	35.0	2.0	35.0
120-121	27.846649048390763	34.0	29.0	35.0	2.0	35.0
122-123	27.719559991896993	34.0	29.0	35.0	2.0	35.0
124-125	26.870271290156822	33.5	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	71036.0
3	12177.0
4	7882.0
5	6798.0
6	7210.0
7	8032.0
8	8937.0
9	9254.0
10	9595.0
11	10262.0
12	10187.0
13	10876.0
14	11853.0
15	9895.0
16	7325.0
17	6345.0
18	5860.0
19	5465.0
20	5279.0
21	5450.0
22	6549.0
23	8655.0
24	12425.0
25	17481.0
26	20819.0
27	21960.0
28	25478.0
29	30379.0
30	34626.0
31	41858.0
32	51972.0
33	68346.0
34	96352.0
35	151545.0
36	286677.0
37	407383.0
38	106833.0
39	96.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.73752990253257	17.03103685178229	12.255157569047666	24.97627567663747
2	16.907429463831217	19.06373089259327	38.23371700528311	25.795122638292405
3	19.536941570672347	23.689690682800837	28.45321632188609	28.320151424640727
4	13.577445471703358	14.885074336408932	35.07572645189988	36.46175373998783
5	14.658738126496255	36.78152753108348	33.0898138852421	15.46992045717816
6	34.08766520640374	34.076172587013346	17.031505842081522	14.804656364501401
7	30.22153902805057	30.81553077741003	20.35450445802732	18.60842573651208
8	27.92081740381122	33.41047876297118	19.177394123767137	19.491309709450462
9	27.78355416428043	14.78662275377334	18.750706603429133	38.679116478517095
10-11	26.588441503037703	24.694183249406652	26.55236039344473	22.165014854110915
12-13	26.84119527600746	22.3644243116765	26.75275401993774	24.041626392378298
14-15	24.292885297352065	23.532855148212676	24.02070899902386	28.1535505554114
16-17	24.454776693066727	26.572403690447725	24.5674696658814	24.405349950604148
18-19	24.532566053102002	25.650335494578762	26.21334476879947	23.60375368351977
20-21	25.561854169743704	24.637939547723384	25.910488953873173	23.88971732865974
22-23	25.415180256977653	24.217082037455977	25.758879991200494	24.608857714365868
24-25	24.876638066330077	24.71379320680463	25.796881474589554	24.612687252275734
26-27	24.488740441447483	24.816444587660317	25.96107652063282	24.73373845025938
28-29	24.50967670577389	24.818042340106196	25.995893401451216	24.676387552668704
30-31	24.895226272631973	24.712696768381242	25.79353836904265	24.598538589944134
32-33	24.4968354860597	24.844091609619724	25.788038742895136	24.871034161425435
34-35	24.80416028489821	24.823127961382554	25.92081273095618	24.451899022763065
36-37	24.92842952787896	24.67449570082832	25.709015747666587	24.688059023626135
38-39	24.891714565217526	24.9288860242112	25.639315105825876	24.540084304745402
40-41	24.85196040661109	24.75961620154208	25.854276580934954	24.534146810911878
42-43	24.759557673251802	24.801764659898513	25.68842036181213	24.750257305037554
44-45	24.65436990639005	24.890557679995847	25.652019152097015	24.80305326151709
46-47	24.830773642403834	24.861643199828933	25.551310519319276	24.756272638447957
48-49	24.924500094075587	25.036340363925767	25.230670556565997	24.80848898543265
50-51	24.836785268393268	25.29827567509617	25.10360130590323	24.761337750607336
52-53	25.014715556405616	25.08467045341835	25.28847897846075	24.61213501171528
54-55	24.838683765806458	25.06773644421514	25.37760943645132	24.715970353527084
56-57	24.74826660553741	25.151744423680135	25.185851583804908	24.914137386977554
58-59	24.85180831393436	24.976342777171052	25.261403430181456	24.910445478713132
60-61	24.908332236718138	25.040204075454543	25.422011507354735	24.62945218047259
62-63	24.671029258114366	25.266380262187997	25.349175171166117	24.713415308531523
64-65	24.764492445809243	24.938638129470846	25.432931359661655	24.863938065058257
66-67	24.74547306886051	25.148199884961674	25.31986153388966	24.786465512288157
68-69	24.814692399886326	25.140548354812005	25.28783067475566	24.75692857054601
70-71	24.644539952624893	24.98676901716274	25.46323887662854	24.905452153583827
72-73	24.874843351476812	25.159119685495174	25.235385932228994	24.730651030799024
74-75	24.706903273600815	25.377063725329673	25.188307888881816	24.727725112187695
76-77	24.702911054643362	25.08184425897816	25.290076812203953	24.92516787417453
78-79	24.6480487378438	25.094293345129397	25.324829376153374	24.932828540873423
80-81	24.68359767245259	25.326270836994773	25.12497795415143	24.86515353640121
82-83	24.838576371151113	25.258250009111254	25.08828650972248	24.814887110015153
84-85	24.65877306555708	25.286651627313965	25.09284288241631	24.961732424712636
86-87	24.444965378959786	25.37659395923924	25.235107733066975	24.943332928733998
88-89	24.64835063009637	25.195824067210282	25.161261428218435	24.99456387447492
90-91	24.616695533148686	25.15083869278074	25.219926767258737	25.012539006811828
92-93	24.680235574927355	25.395898811994705	25.095747099171756	24.828118513906187
94-95	24.657908667343243	25.0736747956532	25.215465190473513	25.052951346530044
96-97	24.660746609164715	25.123574740442862	25.192724092233316	25.022954558159118
98-99	24.545063448527614	25.206587195284463	25.110601396735433	25.137747959452483
100-101	24.559138177676427	25.238636728623064	25.228136341961772	24.97408875173874
102-103	24.560338888101395	25.022150205103642	25.381124041457404	25.036386865337562
104-105	24.441350055387094	25.302745204855043	25.24110448071859	25.014800259039276
106-107	24.447476445714894	25.23128102945845	25.241503224778356	25.0797393000483
108-109	24.465475079387335	25.16370066975054	25.20771037721106	25.163113873651067
110-111	24.523882759719683	25.30864083150817	25.24573294940069	24.921743459371452
112-113	24.47116156687379	25.26466627960124	25.26713690998258	24.99703524354239
114-115	24.288211387135426	25.48822744623291	25.249595434275058	24.97396573235661
116-117	24.276893800305675	25.482238080046844	25.180361566224914	25.060506553422567
118-119	24.440848878292734	25.17970779954447	25.216327268472398	25.163116053690395
120-121	24.150502737754483	25.274457252725046	25.3936800453214	25.181359964199075
122-123	24.250464641959276	25.38355162830867	25.28470806708636	25.08127566264569
124-125	24.224658975945808	25.343216638441007	25.285066833673646	25.14705755193954
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	83.0
1	97.0
2	128.5
3	139.5
4	130.0
5	121.0
6	128.0
7	167.5
8	228.5
9	299.0
10	334.0
11	371.0
12	433.5
13	523.0
14	610.5
15	685.0
16	793.0
17	901.0
18	982.5
19	1033.0
20	1121.0
21	1313.5
22	1537.0
23	1808.0
24	2127.5
25	2601.0
26	3531.5
27	4698.0
28	5571.0
29	6673.5
30	8505.0
31	10234.0
32	12240.0
33	14662.0
34	17198.5
35	19906.5
36	23156.5
37	26569.0
38	28627.0
39	30945.5
40	33642.0
41	35853.5
42	38493.0
43	43056.0
44	47772.5
45	51165.0
46	54711.5
47	57743.5
48	61565.0
49	66047.5
50	66853.5
51	66554.5
52	66881.0
53	67365.0
54	68746.0
55	68181.0
56	64400.0
57	59219.0
58	55597.5
59	51945.5
60	46654.5
61	41497.5
62	36216.0
63	28708.0
64	21961.5
65	17737.0
66	13875.0
67	10770.5
68	8796.0
69	7232.5
70	6176.5
71	5047.0
72	4021.5
73	3603.0
74	2954.0
75	2324.0
76	1702.5
77	1061.5
78	653.0
79	418.0
80	258.0
81	126.0
82	79.0
83	52.5
84	37.5
85	30.5
86	21.5
87	11.5
88	13.5
89	14.0
90	9.5
91	9.0
92	12.5
93	12.0
94	7.5
95	9.5
96	9.0
97	9.5
98	12.0
99	9.5
100	18.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034586005513997445
2	0.02519837544591243
3	0.024271964583930352
4	0.03069507989367274
5	0.03254790161763689
6	0.044591242823403855
7	0.0377358024447365
8	0.03483304841052601
9	0.030448036997144188
10-11	0.03560505746217773
12-13	0.03480216804845993
14-15	0.032856705238297576
16-17	0.036747630858622295
18-19	0.04909977568504995
20-21	0.06071079182189196
22-23	0.054287676512149566
24-25	0.06469435852841487
26-27	0.0476483986679447
28-29	0.04823512554720002
30-31	0.04838952735753036
32-33	0.05521408737413164
34-35	0.03761228099647223
36-37	0.05045851161595699
38-39	0.060216706028834846
40-41	0.04635142346116979
42-43	0.05783891814974752
44-45	0.05854916647726711
46-47	0.06503404251114163
48-49	0.047710159392076845
50-51	0.049933545460833825
52-53	0.05969173987371167
54-55	0.04613526092670731
56-57	0.04468388390960207
58-59	0.044745644633734205
60-61	0.033041987410693995
62-63	0.042923703271836124
64-65	0.041966412047787977
66-67	0.03464776623812959
68-69	0.030386276273012044
70-71	0.048883613150587465
72-73	0.029676027945492456
74-75	0.040700317203079144
76-77	0.032671423065901165
78-79	0.03291846596242972
80-81	0.02263530539442869
82-83	0.016984199136338033
84-85	0.017787088550055834
86-87	0.01645923298121486
88-89	0.021739774894512683
90-91	0.012599187722956215
92-93	0.018775260136170042
94-95	0.01315503424014546
96-97	0.011888939395436624
98-99	0.010036117671472474
100-101	0.010128758757670681
102-103	0.005712866982222793
104-105	0.005712866982222793
106-107	0.007781851240649426
108-109	0.011796298309238416
110-111	0.007287765447592321
112-113	0.008152415585442257
114-115	0.008152415585442257
116-117	0.006948081464865558
118-119	0.24031097759815012
120-121	0.3573475498285522
122-123	0.026711513187149816
124-125	0.0035821219996640214
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1619152.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.97192100516148
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.46862228367155	47.45389236706855
2	10.099571386261527	11.91182251953208
3	3.41384017597286	6.039621395451541
4	1.7231262104709975	4.064642510632756
5	1.0065860278015661	2.9680155858206616
6	0.6785039543870759	2.400760895988259
7	0.47222189749473503	1.949348270517688
8	0.35459973578752313	1.6729142085850357
9	0.25209170002082887	1.3379698637716664
>10	1.4380465744887068	14.908692705295392
>50	0.06962022335112511	2.751785567850843
>100	0.022432683504468014	2.1613431172264175
>500	4.212267355024206E-4	0.13979019383805147
>1k	3.159200516268154E-4	0.23940079842100775
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	6.17607241321383E-5
6	0.0	0.0	0.0	0.0	6.17607241321383E-5
7	0.0	0.0	0.0	0.0	6.17607241321383E-5
8	0.0	0.0	0.0	0.0	6.17607241321383E-5
9	0.0	0.0	0.0	6.17607241321383E-5	1.235214482642766E-4
10-11	0.0	0.0	0.0	6.17607241321383E-5	1.235214482642766E-4
12-13	0.0	0.0	0.0	6.17607241321383E-5	7.102483275195906E-4
14-15	0.0	0.0	0.0	6.17607241321383E-5	8.337697757838671E-4
16-17	0.0	0.0	0.0	6.17607241321383E-5	9.881715861142129E-4
18-19	0.0	0.0	0.0	6.17607241321383E-5	9.881715861142129E-4
20-21	0.0	0.0	0.0	6.17607241321383E-5	9.881715861142129E-4
22-23	0.0	0.0	0.0	6.17607241321383E-5	0.001019051948180282
24-25	0.0	0.0	0.0	1.235214482642766E-4	0.0010499323102463511
26-27	0.0	0.0	0.0	1.5440181033034577E-4	0.0012352144826427661
28-29	0.0	0.0	0.0	7.102483275195906E-4	0.0012352144826427661
30-31	0.0	0.0	0.0	0.0030880362066069153	0.0012660948447088352
32-33	0.0	0.0	0.0	0.009233228257754677	0.0012969752067749044
34-35	0.0	0.0	0.0	0.01673715623980948	0.001420496655039181
36-37	0.0	0.0	0.0	0.02698943644574444	0.001420496655039181
38-39	0.0	0.0	0.0	0.044467721375139574	0.0014513770171052502
40-41	0.0	0.0	0.0	0.06515756395940592	0.0014822573791713194
42-43	0.0	0.0	0.0	0.08421074735417058	0.0014822573791713194
44-45	0.0	0.0	0.0	0.10582700080041899	0.0014822573791713194
46-47	0.0	0.0	0.0	0.13019160647054756	0.0014822573791713194
48-49	0.0	0.0	0.0	0.15671783748530094	0.0015440181033034577
50-51	0.0	3.088036206606915E-5	0.0	0.1854056938446792	0.0015440181033034577
52-53	0.0	6.17607241321383E-5	0.0	0.214340593100586	0.0017293002756998727
54-55	0.0	6.17607241321383E-5	0.0	0.24673409290789253	0.0018528217239641492
56-57	0.0	6.17607241321383E-5	0.0	0.28487134005948794	0.0018528217239641492
58-59	0.0	6.17607241321383E-5	0.0	0.3272391968141348	0.0018837020860302182
60-61	0.0	6.17607241321383E-5	0.0	0.37352885955117243	0.0019763431722284257
62-63	0.0	6.17607241321383E-5	0.0	0.42009644554680475	0.0019763431722284257
64-65	0.0	6.17607241321383E-5	0.0	0.4728092235935848	0.0020998646204927023
66-67	0.0	6.17607241321383E-5	0.0	0.531389270432918	0.0020998646204927023
68-69	0.0	6.17607241321383E-5	0.0	0.5982143739438917	0.0020998646204927023
70-71	0.0	6.17607241321383E-5	0.0	0.6729448501437789	0.002223386068756979
72-73	0.0	6.17607241321383E-5	0.0	0.7498369516882911	0.0025321896894176703
74-75	0.0	6.17607241321383E-5	0.0	0.8312993468185816	0.002593950413549809
76-77	0.0	6.17607241321383E-5	0.0	0.9194936608792751	0.002593950413549809
78-79	0.0	6.17607241321383E-5	0.0	1.0212135735249068	0.002593950413549809
80-81	0.0	6.17607241321383E-5	0.0	1.1326916805834164	0.0026557111376819473
82-83	0.0	6.17607241321383E-5	0.0	1.2567689753648823	0.0027174718618140853
84-85	0.0	6.17607241321383E-5	0.0	1.3831314169392375	0.002779232585946224
86-87	0.0	6.17607241321383E-5	0.0	1.5201167030643201	0.002995395120408708
88-89	0.0	6.17607241321383E-5	0.0	1.6697938179985572	0.0030880362066069153
90-91	0.0	6.17607241321383E-5	0.0	1.835405199758886	0.0030880362066069153
92-93	0.0	6.17607241321383E-5	0.0	2.0145730604662195	0.0033350791031354684
94-95	0.0	6.17607241321383E-5	0.0	2.2053210569483284	0.003458600551399745
96-97	0.0	6.17607241321383E-5	0.0	2.40783447137761	0.003613002361730091
98-99	0.0	6.17607241321383E-5	0.0	2.629678992460251	0.003798284534126506
100-101	0.0	6.17607241321383E-5	0.0	2.8774630176783895	0.003829164896192575
102-103	0.0	6.17607241321383E-5	0.0	3.1502601361700444	0.003860045258258644
104-105	0.0	6.17607241321383E-5	0.0	3.4292950877990456	0.003890925620324713
106-107	0.0	6.17607241321383E-5	0.0	3.721083628961333	0.003921805982390782
108-109	0.0	9.264108619820745E-5	0.0	4.033469371621688	0.004076207792721128
110-111	0.0	1.235214482642766E-4	0.0	4.376642835261915	0.004076207792721128
112-113	0.0	1.235214482642766E-4	0.0	4.751468670019863	0.004076207792721128
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1800	0.0	69.7603	1
GTATCAA	3735	0.0	59.43149	1
TCAACGC	4500	0.0	48.121555	4
CAACGCA	4670	0.0	46.36981	5
AACGCAG	4960	0.0	43.65867	6
ATCAACG	4955	0.0	43.581314	3
TATCAAC	5275	0.0	41.726948	2
ACGCAGA	5785	0.0	37.328506	7
CGCAGAG	6245	0.0	34.674187	8
GTACATG	8235	0.0	34.54321	1
TACATGG	8495	0.0	33.123417	2
ACATGGG	8510	0.0	32.6456	3
GCAGAGT	6725	0.0	31.313736	9
CATGGGG	7130	0.0	30.204372	4
ATGGGGG	3870	0.0	27.20903	5
CAGAGTA	6365	0.0	26.449575	10-11
AGAGTAC	6010	0.0	26.032263	10-11
ATGGGGA	2880	0.0	22.72223	5
TATACCG	220	4.1846943E-6	21.633066	5
GTGGTAT	875	0.0	20.403826	1
>>END_MODULE