FastQCFastQC Report
Mon 15 Aug 2016
SRR2921478_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921478_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1619152
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27560.17021255570817317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24850.1534753994683637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23400.078.729091
GTATCAA48300.059.743521
TCAACGC64750.043.5853044
CAACGCA67600.041.8112755
ATCAACG69900.040.5444373
AACGCAG70500.040.251486
TATCAAC70150.040.1453252
GTACATG77400.035.818271
ACGCAGA81250.034.7773137
TACATGG83100.032.9578062
CGCAGAG86000.032.78738
ACATGGG81700.032.575193
CATGGGG67250.031.1639064
GCAGAGT91100.030.9498779
GTGGTAT8750.029.2990131
AGAGTAC84950.027.344510-11
ATGGGGG36500.026.899095
CAGAGTA88950.025.98109410-11
GAGTACT50750.025.08438712-13
GTACTTT53350.024.08603114-15
Produced by FastQC (version 0.11.5)