##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921478_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1619152
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.946798694625336	31.0	31.0	34.0	28.0	34.0
2	31.0129092265581	31.0	31.0	34.0	27.0	34.0
3	31.08916828068026	33.0	31.0	34.0	27.0	34.0
4	34.58969571726435	37.0	35.0	37.0	31.0	37.0
5	34.21545537417117	37.0	35.0	37.0	30.0	37.0
6	34.189838261015645	37.0	35.0	37.0	30.0	37.0
7	34.012922196310164	37.0	35.0	37.0	30.0	37.0
8	33.98561283931342	37.0	35.0	37.0	30.0	37.0
9	35.51255842564503	39.0	35.0	39.0	30.0	39.0
10-11	35.37143671502119	39.0	35.0	39.0	29.0	39.0
12-13	35.27302254513474	39.0	35.0	39.0	28.0	39.0
14-15	36.39743797988083	40.0	36.0	41.0	28.0	41.0
16-17	36.37442933090902	40.0	36.0	41.0	28.0	41.0
18-19	36.38957614850243	40.0	36.0	41.0	28.0	41.0
20-21	36.36953911677224	40.0	36.0	41.0	27.5	41.0
22-23	36.30763325493839	40.0	36.0	41.0	27.0	41.0
24-25	36.23107805814402	40.0	36.0	41.0	27.0	41.0
26-27	36.097350959020524	40.0	36.0	41.0	27.0	41.0
28-29	35.90190019219938	40.0	35.5	41.0	27.0	41.0
30-31	35.85011907467613	40.0	35.0	41.0	27.0	41.0
32-33	35.721381006848034	40.0	35.0	41.0	27.0	41.0
34-35	35.591159755229896	40.0	35.0	41.0	26.0	41.0
36-37	35.44978359042264	40.0	35.0	41.0	26.0	41.0
38-39	35.26534506951787	39.0	35.0	41.0	25.0	41.0
40-41	35.05248117533128	39.0	35.0	41.0	24.0	41.0
42-43	34.87282108165262	39.0	35.0	41.0	24.0	41.0
44-45	34.64007455754617	39.0	34.0	41.0	23.0	41.0
46-47	34.34221987805962	39.0	34.0	41.0	21.0	41.0
48-49	34.06290669436841	39.0	33.0	41.0	18.5	41.0
50-51	33.828742452839506	38.0	33.0	41.0	17.5	41.0
52-53	33.628147017698154	38.0	33.0	40.0	15.5	41.0
54-55	33.409632943664334	38.0	33.0	40.0	14.5	41.0
56-57	33.14721378845223	37.0	33.0	40.0	13.0	41.0
58-59	33.00029583386859	37.0	33.0	40.0	12.5	41.0
60-61	32.88404454924553	36.5	33.0	40.0	11.5	41.0
62-63	32.62112358814984	36.0	32.5	39.5	8.0	41.0
64-65	32.327383099301365	35.5	32.0	39.0	8.0	41.0
66-67	32.02691624998765	35.0	32.0	39.0	7.0	41.0
68-69	31.714107137563367	35.0	32.0	38.5	6.0	40.5
70-71	31.422133623032302	35.0	32.0	37.0	2.0	40.0
72-73	31.114706957716137	35.0	31.0	37.0	2.0	39.0
74-75	30.829634895303222	35.0	31.0	36.0	2.0	39.0
76-77	30.550469937349924	35.0	31.0	36.0	2.0	38.5
78-79	30.28294224384122	35.0	31.0	35.5	2.0	37.0
80-81	30.069151012381788	35.0	31.0	35.0	2.0	37.0
82-83	29.886741948872	35.0	31.0	35.0	2.0	36.5
84-85	29.70207954534225	35.0	30.0	35.0	2.0	36.0
86-87	29.56625412561637	35.0	30.0	35.0	2.0	36.0
88-89	29.4623932774687	35.0	30.0	35.0	2.0	35.5
90-91	29.363890480943112	35.0	30.0	35.0	2.0	35.0
92-93	29.427819315295906	35.0	30.5	35.0	2.0	35.0
94-95	29.400377481545895	35.0	31.0	35.0	2.0	35.0
96-97	29.381719566785577	35.0	31.0	35.0	2.0	35.0
98-99	29.342989416682315	35.0	31.0	35.0	2.0	35.0
100-101	29.290958785833574	35.0	31.0	35.0	2.0	35.0
102-103	28.41241958753718	33.5	29.0	35.0	2.0	35.0
104-105	29.11732159797227	34.5	30.0	35.0	2.0	35.0
106-107	29.22593833068174	35.0	31.0	35.0	2.0	35.0
108-109	29.229398784054865	35.0	31.0	35.0	2.0	35.0
110-111	29.19664089597518	35.0	31.0	35.0	2.0	35.0
112-113	29.15065602241173	35.0	31.0	35.0	2.0	35.0
114-115	29.086549317173436	35.0	31.0	35.0	2.0	35.0
116-117	29.011962125853533	35.0	30.5	35.0	2.0	35.0
118-119	28.91704237773847	35.0	30.0	35.0	2.0	35.0
120-121	28.79431424597567	35.0	30.0	35.0	2.0	35.0
122-123	28.672692248782077	35.0	30.0	35.0	2.0	35.0
124-125	27.75088348715871	34.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	36338.0
3	12613.0
4	6961.0
5	5796.0
6	6006.0
7	6934.0
8	7858.0
9	8551.0
10	9511.0
11	10210.0
12	10748.0
13	12304.0
14	15260.0
15	12815.0
16	7084.0
17	6068.0
18	5624.0
19	4850.0
20	4719.0
21	4964.0
22	6059.0
23	8063.0
24	11224.0
25	15891.0
26	19357.0
27	21596.0
28	24599.0
29	29393.0
30	34094.0
31	41405.0
32	51591.0
33	65748.0
34	92043.0
35	146909.0
36	287733.0
37	438611.0
38	129394.0
39	228.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.74810855730966	17.642355949851726	12.617264500236198	24.99227099260241
2	17.22156446035425	19.64490740998081	37.11197453936597	26.021553590298968
3	19.6239599658269	23.942236830969776	28.76890964204107	27.664893561162256
4	13.770529082906071	15.570124078249817	34.64594225995564	36.01340457888847
5	14.584921536091175	36.88365892262091	33.03842699044793	15.492992550839993
6	34.095747112377886	34.68426729330136	16.27749094085394	14.942494653466815
7	29.943819357150137	31.317789713290324	20.00364448370329	18.734746445856253
8	28.154511686396198	33.481546722732055	18.737454155554264	19.626487435317486
9	27.833398596008397	14.504866289748982	18.53217038128637	39.129564732956254
10-11	26.690363763823736	24.770435658057682	26.206312980800412	22.33288759731817
12-13	27.042380266806003	22.379142710145704	26.76064602159623	23.81783100145206
14-15	24.476943064143512	23.37596885699348	24.29242828551338	27.854659793349622
16-17	24.267801034346657	26.4257782461073	24.85582087136881	24.45059984817723
18-19	24.319061882072535	25.113004583182395	27.09027983013721	23.477653704607857
20-21	25.338736397114687	24.105535782892453	26.923984439373967	23.631743380618893
22-23	25.15434677135677	23.726274327895513	26.8529256167617	24.266453283986014
24-25	24.583588518652697	24.317639860857984	26.80015677103553	24.298614849453788
26-27	24.27032569286169	24.37659433851551	26.984206397736887	24.368873570885913
28-29	24.38459580860516	24.38462669308731	27.005082350383212	24.225695147924316
30-31	24.59434181971832	24.30149945422164	26.84827979434009	24.255878931719945
32-33	24.317357760990284	24.364734732199665	26.772744961178063	24.54516254563199
34-35	24.486388265915828	24.392646306180698	26.90530147074494	24.215663957158533
36-37	24.7013545675965	24.25339414031687	26.649846530931935	24.39540476115469
38-39	24.690426454913037	24.463499031226537	26.565334372786747	24.28074014107368
40-41	24.641225608159193	24.401229413014583	26.684266181753497	24.27327879707273
42-43	24.66219127171363	24.434334640415493	26.422289554573947	24.481184533296933
44-45	24.594808958863798	24.496499401432857	26.29671016625218	24.61198147345117
46-47	24.748398661914823	24.618926474190033	26.007941908003712	24.62473295589143
48-49	24.98924355223155	24.897354299938257	25.39961014247313	24.713792005357067
50-51	24.892551160966327	25.132712751172086	25.284141799111747	24.690594288749836
52-53	25.016741064534028	25.040926034146832	25.374172058607314	24.56816084271183
54-55	24.878355681378057	25.025002177459555	25.467350769894104	24.629291371268284
56-57	24.757018712964406	25.06836092840519	25.315735908907694	24.85888444972271
58-59	24.75533261699513	24.87541552609008	25.53596418267798	24.83328767423681
60-61	24.852453709938263	24.954040427127513	25.683216231142058	24.510289631792165
62-63	24.72021411153135	25.03643942283784	25.63599495299708	24.607351512633723
64-65	24.738000984030965	24.77599087995409	25.699763072771038	24.78624506324391
66-67	24.785200284134778	24.975632354303716	25.54618734364866	24.692980017912845
68-69	24.801645706660818	24.977583115445025	25.451156210859256	24.769614967034904
70-71	24.667219512044465	24.818618843997225	25.69063205051586	24.82352959344245
72-73	24.95109950066359	25.098272012482948	25.316575331169037	24.634053155684427
74-75	24.72332074408744	25.268463506630045	25.26330549862185	24.744910250660656
76-77	24.729312120158305	24.98580853662077	25.335298416053558	24.94958092716737
78-79	24.7367855276213	25.065425214469904	25.261824418246903	24.93596483966189
80-81	24.768083380642246	25.192411877244343	25.194697530814626	24.84480721129879
82-83	24.842248496295866	25.15725737213419	25.158369168166562	24.842124963403382
84-85	24.64186531467426	25.153526002134726	25.118070791824792	25.086537891366223
86-87	24.481124937072828	25.284276391578413	25.276400843764574	24.958197827584183
88-89	24.569352268624232	25.090290005970427	25.238176279325913	25.102181446079424
90-91	24.5964076879197	24.986171417594157	25.317810318485144	25.09961057600099
92-93	24.614199175109366	25.238719520198394	25.232820826403913	24.914260478288327
94-95	24.559071590529374	24.963017911375164	25.31606946641409	25.161841031681377
96-97	24.578995023167163	25.02807111596428	25.213235484855183	25.17969837601337
98-99	24.476784977555766	25.049903528080613	25.127507219953504	25.34580427441011
100-101	24.47215918426342	25.082491534657564	25.30587651038881	25.13947277069021
102-103	24.524564061483805	24.84736824876644	25.4349503085987	25.193117381151055
104-105	24.495065220613665	25.069821878551313	25.23620855773507	25.198904343099954
106-107	24.47049937204454	25.042777246118085	25.18646432512004	25.300259056717334
108-109	24.488145053983832	24.977915985614196	25.161074314716046	25.372864645685926
110-111	24.486750089399145	25.061097135954018	25.1773932259226	25.274759548724234
112-113	24.400966396922552	25.013071312075606	25.271625416498484	25.314336874503358
114-115	24.21702983652261	25.256296155387826	25.234401586537896	25.292272421551665
116-117	24.193347648564274	25.27906516374242	25.125052385036273	25.40253480265704
118-119	24.23720584910765	24.912692049695426	25.272918414163964	25.57718368703296
120-121	23.99567781768704	25.050437298246948	25.410176522051437	25.543708362014577
122-123	24.017982374604806	25.09055727697588	25.311229843478735	25.58023050494058
124-125	24.03101517461557	25.208524323616405	25.225142022639606	25.535318479128417
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	30.0
1	28.5
2	47.0
3	74.0
4	62.0
5	54.5
6	98.5
7	214.0
8	382.5
9	467.5
10	431.5
11	418.5
12	485.5
13	594.0
14	730.5
15	887.0
16	1010.5
17	1159.5
18	1398.5
19	1586.5
20	1760.5
21	2026.0
22	2254.0
23	2562.5
24	2907.5
25	3290.5
26	4156.5
27	5211.5
28	6010.0
29	7105.0
30	8889.5
31	10536.0
32	12348.5
33	14598.0
34	17103.0
35	19583.5
36	22599.0
37	25757.0
38	27917.5
39	30572.0
40	33141.5
41	35420.0
42	37832.0
43	42073.5
44	46996.5
45	50645.0
46	54255.5
47	57405.0
48	61768.0
49	65950.0
50	66518.0
51	66635.0
52	67024.5
53	67565.0
54	68742.0
55	68083.5
56	64908.5
57	59681.0
58	55663.0
59	52020.5
60	46794.0
61	41516.5
62	35894.5
63	28566.5
64	21857.0
65	17410.5
66	13609.5
67	10663.0
68	8668.0
69	7041.5
70	5944.0
71	4886.0
72	3956.0
73	3556.5
74	2919.5
75	2313.5
76	1632.5
77	928.0
78	564.0
79	342.0
80	195.5
81	100.0
82	59.5
83	30.0
84	13.0
85	7.5
86	5.0
87	4.5
88	3.0
89	2.5
90	1.5
91	1.5
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11536903267883436
2	0.0015440181033034577
3	0.02068984258426633
4	0.05552289099479234
5	0.02754528296293368
6	0.021369210549719853
7	0.01649011334328093
8	0.005558465171892448
9	0.003335079103135469
10-11	0.006608397482138798
12-13	0.004508532861646096
14-15	0.0025013093273516013
16-17	0.00981995513700999
18-19	0.004971738292637133
20-21	0.011981580481634831
22-23	0.009356749706018954
24-25	0.01423584691245788
26-27	0.008770022826763639
28-29	0.013340316412541875
30-31	0.022419142859966205
32-33	0.013710880757334702
34-35	0.02140009091178592
36-37	0.01599602755022382
38-39	0.02177065525657875
40-41	0.010375801654199235
42-43	0.009356749706018954
44-45	0.017663567101791552
46-47	0.01676803660187555
48-49	0.021955937428975166
50-51	0.014946095239977471
52-53	0.02334555372194828
54-55	0.018095892170716523
56-57	0.02189417670484303
58-59	0.016706275877743412
60-61	0.020875124756662745
62-63	0.022820587566825104
64-65	0.018559097601707562
66-67	0.012475666274691936
68-69	0.02436460567012856
70-71	0.0157181042916292
72-73	0.018744379774103977
74-75	0.019145824480962872
76-77	0.013649120033202566
78-79	0.03149796930739054
80-81	0.02217209996343765
82-83	0.009264108619820747
84-85	0.012846230619484766
86-87	0.01340207713667401
88-89	0.02102952656699309
90-91	0.013741761119400774
92-93	0.009233228257754677
94-95	0.007720090516517288
96-97	0.0030880362066069153
98-99	0.0017293002756998727
100-101	0.012228623378163385
102-103	0.0036438827237961604
104-105	0.0019763431722284257
106-107	5.249661551231756E-4
108-109	0.020628081860134193
110-111	5.558465171892448E-4
112-113	0.00917146753362254
114-115	0.0017910609998320107
116-117	0.00805977449924405
118-119	0.010499323102463511
120-121	0.003860045258258644
122-123	6.793679654535213E-4
124-125	0.02445724675632677
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1619152.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.371081726895156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.64250441743673	45.69176629867372
2	10.436572771945638	11.97514938895964
3	3.625456489877853	6.239890817342545
4	1.793737312441784	4.116345997947154
5	1.0572182202940195	3.032687645982542
6	0.68590382256415	2.3610626556670584
7	0.4990078655500987	2.0040034725786757
8	0.3668841742964978	1.683883355829506
9	0.2739414726697898	1.4144686755232074
>10	1.5179225792539655	15.514512184697196
>50	0.07426147111816918	2.858945168883078
>100	0.02594094075444705	2.5748480506537317
>500	3.2423089839629356E-4	0.12971430219540803
>1k	3.2423089839629356E-4	0.4027219850665273
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2756	0.17021255570817317	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2485	0.1534753994683637	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.17607241321383E-5
2	0.0	0.0	0.0	0.0	6.17607241321383E-5
3	0.0	0.0	0.0	0.0	6.17607241321383E-5
4	0.0	0.0	0.0	0.0	6.17607241321383E-5
5	0.0	0.0	0.0	0.0	6.17607241321383E-5
6	0.0	0.0	0.0	0.0	6.17607241321383E-5
7	0.0	0.0	0.0	0.0	6.17607241321383E-5
8	0.0	0.0	0.0	0.0	6.17607241321383E-5
9	0.0	0.0	0.0	0.0	6.17607241321383E-5
10-11	0.0	0.0	0.0	0.0	6.17607241321383E-5
12-13	0.0	0.0	0.0	0.0	5.249661551231756E-4
14-15	0.0	0.0	0.0	0.0	6.793679654535213E-4
16-17	0.0	0.0	0.0	0.0	8.646501378499362E-4
18-19	0.0	0.0	0.0	0.0	9.881715861142129E-4
20-21	0.0	0.0	0.0	0.0	0.0010808126723124202
22-23	0.0	0.0	0.0	6.17607241321383E-5	0.0012352144826427661
24-25	0.0	0.0	0.0	1.235214482642766E-4	0.0012969752067749044
26-27	0.0	0.0	0.0	1.5440181033034577E-4	0.0012969752067749044
28-29	0.0	0.0	0.0	7.720090516517288E-4	0.0013587359309070427
30-31	0.0	0.0	0.0	0.0031497969307390534	0.0013587359309070427
32-33	0.0	0.0	0.0	0.00944939079221716	0.001420496655039181
34-35	0.0	0.0	0.0	0.017323883119064794	0.0014822573791713194
36-37	0.0	0.0	0.0	0.027977608031858652	0.0014822573791713194
38-39	0.0	0.0	0.0	0.04582645730604662	0.0015748984653695267
40-41	0.0	0.0	0.0	0.06633101771791654	0.001605778827435596
42-43	0.0	0.0	0.0	0.08581652618160618	0.0016675395515677342
44-45	0.0	0.0	0.0	0.10740189926578851	0.0016675395515677342
46-47	0.0	0.0	0.0	0.13287819797029557	0.0016984199136338032
48-49	0.0	0.0	0.0	0.16008379695050248	0.0018528217239641492
50-51	0.0	0.0	0.0	0.1886790122236825	0.0018528217239641492
52-53	0.0	0.0	0.0	0.21866384378983567	0.002038103896360564
54-55	0.0	0.0	0.0	0.25186023301086	0.0020998646204927023
56-57	0.0	0.0	0.0	0.2910782928347678	0.0020998646204927023
58-59	0.0	0.0	0.0	0.33400199610660397	0.0020998646204927023
60-61	0.0	0.0	0.0	0.38115630898149155	0.0021616253446248407
62-63	0.0	0.0	0.0	0.4285576647529077	0.0021616253446248407
64-65	0.0	0.0	0.0	0.48114692135142345	0.0021925057066909098
66-67	0.0	0.0	0.0	0.5391093609494353	0.0022233860687569788
68-69	0.0	0.0	0.0	0.6062741484431358	0.0022233860687569788
70-71	0.0	0.0	0.0	0.681467830074014	0.0022851467928891173
72-73	0.0	0.0	0.0	0.7589466584977815	0.002624830775615878
74-75	0.0	0.0	0.0	0.841767789558979	0.0027483522238801548
76-77	0.0	0.0	0.0	0.9318458057057027	0.002840993310078362
78-79	0.0	0.0	0.0	1.0348009328339773	0.002840993310078362
80-81	0.0	0.0	0.0	1.1478230579957902	0.002840993310078362
82-83	0.0	0.0	0.0	1.2749883889838634	0.002840993310078362
84-85	0.0	0.0	0.0	1.4032962933683806	0.002840993310078362
86-87	0.0	0.0	0.0	1.5420726404932952	0.002871873672144431
88-89	0.0	0.0	0.0	1.6939113807721573	0.0029027540342105003
90-91	0.0	0.0	0.0	1.8623637558425645	0.0029027540342105003
92-93	0.0	0.0	0.0	2.0437550026186546	0.0031189165686729844
94-95	0.0	0.0	0.0	2.2362014190143977	0.0032733183790033303
96-97	0.0	0.0	0.0	2.4412161427710304	0.003489480913465814
98-99	0.0	0.0	0.0	2.6670442305601947	0.0037056434479282984
100-101	0.0	0.0	0.0	2.9181942152435347	0.0037056434479282984
102-103	0.0	0.0	0.0	3.193832327045268	0.003860045258258644
104-105	0.0	0.0	0.0	3.4763258792256684	0.0040144470685889895
106-107	0.0	0.0	0.0	3.772314149628942	0.0040144470685889895
108-109	0.0	0.0	0.0	4.090103955650859	0.0041379685168532665
110-111	0.0	0.0	0.0	4.439546132790498	0.004292370327183612
112-113	0.0	0.0	0.0	4.82085684358232	0.0043232506892496815
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2340	0.0	78.72909	1
GTATCAA	4830	0.0	59.74352	1
TCAACGC	6475	0.0	43.585304	4
CAACGCA	6760	0.0	41.811275	5
ATCAACG	6990	0.0	40.544437	3
AACGCAG	7050	0.0	40.25148	6
TATCAAC	7015	0.0	40.145325	2
GTACATG	7740	0.0	35.81827	1
ACGCAGA	8125	0.0	34.777313	7
TACATGG	8310	0.0	32.957806	2
CGCAGAG	8600	0.0	32.7873	8
ACATGGG	8170	0.0	32.57519	3
CATGGGG	6725	0.0	31.163906	4
GCAGAGT	9110	0.0	30.949877	9
GTGGTAT	875	0.0	29.299013	1
AGAGTAC	8495	0.0	27.3445	10-11
ATGGGGG	3650	0.0	26.89909	5
CAGAGTA	8895	0.0	25.981094	10-11
GAGTACT	5075	0.0	25.084387	12-13
GTACTTT	5335	0.0	24.086031	14-15
>>END_MODULE