FastQCFastQC Report
Mon 15 Aug 2016
SRR2921477_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921477_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3611192
Sequences flagged as poor quality0
Sequence length98
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41670.11539126138959102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA66000.038.2855151
GGTATCA40700.029.9679871
TCAACGC84850.029.1242244
TATCAAC87700.028.596392
CAACGCA88000.028.1338125
ATCAACG89250.027.8429053
AACGCAG94300.026.4421236
GTACATG122250.026.0156861
TACATGG124600.024.8375742
GTACCTG37250.023.9707471
ACATGGG127200.023.6810913
ACGCAGA110100.022.3085987
TACCTGG40500.021.0562652
CGCAGAG117300.020.8958648
CATGGGG101950.020.191874
GAGTACA91800.020.0550351
AGTACAT90600.019.128772
GCAGAGT131000.018.4660159
GTATAGG7150.018.0242861
GTGTAGG12400.017.8166331
Produced by FastQC (version 0.11.5)