##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921477_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3611192
Sequences flagged as poor quality	0
Sequence length	98
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.91094962549762	31.0	31.0	34.0	26.0	34.0
2	29.998511017968582	31.0	31.0	34.0	26.0	34.0
3	30.127847259298314	31.0	31.0	34.0	26.0	34.0
4	33.62388679416658	37.0	35.0	37.0	28.0	37.0
5	33.582138529327715	37.0	35.0	37.0	28.0	37.0
6	33.68248572770432	37.0	35.0	37.0	29.0	37.0
7	33.551129100861985	37.0	35.0	37.0	28.0	37.0
8	33.52415656658522	37.0	35.0	37.0	28.0	37.0
9	34.91580536288295	39.0	35.0	39.0	28.0	39.0
10-11	34.77092660816706	38.5	35.0	39.0	27.0	39.0
12-13	34.58680222486093	38.0	35.0	39.0	27.0	39.0
14-15	35.512382753395556	39.0	36.0	41.0	27.0	41.0
16-17	35.340383175416875	39.0	36.0	40.5	26.5	41.0
18-19	35.12958712801756	39.0	35.0	40.0	25.5	41.0
20-21	34.99401374947662	39.0	35.0	40.0	25.0	41.0
22-23	35.01968795898972	39.0	35.0	40.0	24.5	41.0
24-25	34.85707170928602	39.0	35.0	40.0	24.0	41.0
26-27	34.5472182038507	39.0	34.0	40.0	22.5	41.0
28-29	34.272679076604064	38.0	34.0	40.0	19.5	41.0
30-31	33.98845976619354	38.0	33.5	40.0	18.0	41.0
32-33	33.676775978679615	38.0	33.0	40.0	16.5	41.0
34-35	33.22343979494859	38.0	32.5	40.0	12.0	41.0
36-37	32.79972084009934	38.0	32.0	40.0	9.0	41.0
38-39	32.528936567205506	38.0	31.0	40.0	8.5	41.0
40-41	32.2254845491461	37.0	31.0	40.0	8.0	41.0
42-43	31.896281338682627	37.0	30.5	40.0	6.5	41.0
44-45	31.54401607557837	37.0	30.0	39.5	2.0	40.5
46-47	31.217184796599017	36.0	30.0	39.0	2.0	40.0
48-49	30.96461030042158	36.0	29.0	39.0	2.0	40.0
50-51	30.26378353740261	35.0	28.5	38.5	2.0	39.5
52-53	30.849457464460485	36.0	30.0	38.5	2.0	40.0
54-55	31.251280463625307	36.0	30.0	39.0	2.0	40.0
56-57	31.072269073480445	36.0	30.0	39.0	2.0	41.0
58-59	30.711480724370233	36.0	29.5	39.0	2.0	40.0
60-61	30.222691565555085	35.0	29.0	39.0	2.0	40.0
62-63	29.69856213682352	35.0	28.0	38.5	2.0	40.0
64-65	29.113280185600765	34.5	27.5	38.0	2.0	40.0
66-67	28.593230850090496	34.0	26.0	37.0	2.0	39.5
68-69	28.04833487114504	34.0	26.0	36.5	2.0	39.0
70-71	27.489072029401925	33.5	25.0	36.0	2.0	39.0
72-73	26.901556605132043	33.0	23.5	35.5	2.0	38.5
74-75	26.26916541684851	32.5	21.5	35.0	2.0	37.5
76-77	25.6473019158217	32.0	19.5	35.0	2.0	37.0
78-79	25.045738913909865	31.5	18.0	35.0	2.0	36.0
80-81	24.42124248724521	31.0	10.5	34.0	2.0	36.0
82-83	23.74435158806289	31.0	6.0	34.0	2.0	35.0
84-85	23.073606581981792	30.0	2.0	34.0	2.0	35.0
86-87	22.39472700426895	29.5	2.0	34.0	2.0	35.0
88-89	21.679570208396562	29.0	2.0	34.0	2.0	35.0
90-91	20.87475423627434	29.0	2.0	34.0	2.0	35.0
92-93	19.998200594152845	27.0	2.0	33.0	2.0	34.5
94-95	19.02858017518869	25.5	2.0	33.0	2.0	34.0
96-97	18.098928276314304	24.5	2.0	32.0	2.0	34.0
98	17.468459168053098	23.0	2.0	32.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	143908.0
3	24263.0
4	18548.0
5	15699.0
6	15150.0
7	15876.0
8	18153.0
9	20769.0
10	23112.0
11	25320.0
12	27390.0
13	29221.0
14	25978.0
15	26256.0
16	29085.0
17	32519.0
18	36254.0
19	36631.0
20	38758.0
21	41966.0
22	45500.0
23	50651.0
24	55769.0
25	62213.0
26	70291.0
27	78341.0
28	89903.0
29	103337.0
30	121054.0
31	144668.0
32	175900.0
33	221021.0
34	286732.0
35	379839.0
36	473344.0
37	439503.0
38	162381.0
39	5889.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.085527101088005	16.908536906021606	13.202645403862597	24.80329058902779
2	16.39106947631362	18.493677851062262	38.5135206271226	26.60173204550152
3	20.73293592760913	21.845465069433324	26.9128867958928	30.508712207064747
4	14.22346424129379	14.315645921494013	33.83034414771013	37.630545689502064
5	16.286483203206398	35.16771854452912	32.027020736070654	16.51877751619382
6	36.13832605466759	32.190361125274336	16.243155467866725	15.42815735219136
7	32.115081572630785	29.616234072780383	18.726631397206962	19.542052957381877
8	28.417725691184188	33.087210883675425	18.493833643825415	20.00122978131497
9	27.08615364504286	16.645525868582155	18.695897097303803	37.57242338907118
10-11	27.18689159151869	24.591075413120567	25.40139813385509	22.82063486150565
12-13	27.23612085242265	21.606583641843457	26.261771216230343	24.89552428950355
14-15	24.236781341146628	23.245920273018562	23.757885468801973	28.759412917032833
16-17	24.84482612022787	26.3629281008534	24.281959892284636	24.510285886634094
18-19	24.83275125825334	25.560154615987692	25.76163308530548	23.845461040453493
20-21	25.33822193323367	24.773682602981275	25.697397384579652	24.190698079205408
22-23	25.074987813310873	24.420281589097137	25.642983855911407	24.861746741680584
24-25	24.785631980366432	24.718221960618692	25.70741030795502	24.788735751059853
26-27	24.84642265394736	24.890852266963492	25.703722941035277	24.559002138053867
28-29	24.804389111977844	25.021702206917656	25.61180544622842	24.562103234876083
30-31	24.931455645186986	24.914622620205115	25.526541177735407	24.627380556872488
32-33	24.80296294859924	24.90825621361153	25.448725628771296	24.840055209017933
34-35	24.965773591893317	24.917520195249008	25.54810909521614	24.56859711764153
36-37	24.975298986418938	24.897409455510378	25.48446521870168	24.642826339369005
38-39	24.983556614752963	24.933812153942117	25.43366599825449	24.648965233050433
40-41	25.057595600899248	24.90043313674332	25.461642109625483	24.58032915273195
42-43	25.051172502181174	24.801083995296153	25.380335976729683	24.767407525792986
44-45	24.93307454004058	24.899723166899403	25.398782758397974	24.76841953466204
46-47	25.02676124929348	24.90863735239906	25.3466157649681	24.71798563333936
48-49	25.09501300866525	24.955066351499074	25.176286723646406	24.773633916189265
50-51	24.857628225069544	25.172387635386283	25.19763631188379	24.772347827660386
52-53	25.030228735408016	25.074109157774497	25.16092274259605	24.734739364221436
54-55	24.91638814616756	24.94149676916221	25.330394942067734	24.811720142602496
56-57	24.878001452415035	25.071907350564743	25.30694691196157	24.74314428505865
58-59	24.951113373691445	25.016648103827944	25.275975808513333	24.756262713967274
60-61	25.01151426383803	24.91191188000613	25.2940921839827	24.782481672173144
62-63	24.846182318863054	25.09409191712255	25.278538105956194	24.781187658058204
64-65	24.91255662134546	24.922873561650054	25.30189929162579	24.8626705253787
66-67	24.942165824587704	24.982790059053457	25.244305957167267	24.830738159191572
68-69	24.91986202403722	24.966577856472703	25.27723257413146	24.836327545358625
70-71	24.820470539359857	24.839753912987465	25.35897196523038	24.9808035824223
72-73	25.017680378813594	24.906548556357425	25.166593404147587	24.909177660681397
74-75	24.83236616378486	25.109659294389257	25.179267686338047	24.878706855487835
76-77	24.983770630286543	24.87635842293508	25.166073711036123	24.973797235742254
78-79	24.937242195054374	24.804925041143083	25.309699752863324	24.94813301093922
80-81	24.881964235295424	24.99049343720314	25.198497309371508	24.929045018129926
82-83	25.016679724799847	24.900269435287676	25.14011525286407	24.94293558704841
84-85	24.962462603407758	24.935358635283915	25.11074870505195	24.991430056256377
86-87	24.937675663121766	24.848128322910018	25.266437757019222	24.947758256948994
88-89	24.8838371993059	24.728577871295983	25.254116994393083	25.133467935005033
90-91	24.959641206957052	24.775590924080408	25.166139699352545	25.098628169609995
92-93	25.006661804522906	24.8563609246084	25.22385888434384	24.91311838652485
94-95	25.050164032840506	24.734588938681235	25.178074318713602	25.03717270976466
96-97	25.075161658763847	24.716029684126315	25.211242648650124	24.99756600845972
98	25.008455935120725	24.76331726334786	25.054490713491777	25.17373608803964
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	503.0
1	382.0
2	417.5
3	565.5
4	465.0
5	430.5
6	746.5
7	1473.5
8	2081.5
9	2015.0
10	1616.5
11	1418.5
12	1503.0
13	1724.5
14	2006.5
15	2329.5
16	2557.0
17	2647.0
18	2641.5
19	2609.0
20	2515.0
21	2292.0
22	2219.0
23	2308.5
24	2398.5
25	2707.5
26	3342.0
27	4257.0
28	5637.5
29	7614.0
30	10691.0
31	14834.5
32	19817.0
33	25575.0
34	32136.0
35	39478.5
36	47432.5
37	56416.0
38	65446.5
39	74327.5
40	83918.5
41	93730.0
42	104695.0
43	116040.5
44	125994.0
45	136911.5
46	147939.0
47	157358.0
48	163212.0
49	164114.5
50	163760.0
51	164715.0
52	166577.0
53	167541.0
54	165876.0
55	159957.0
56	150741.0
57	140629.0
58	129510.5
59	116425.0
60	102325.5
61	86781.0
62	70929.5
63	55942.5
64	42505.0
65	32576.5
66	25294.5
67	19721.0
68	15359.0
69	12179.5
70	9494.0
71	7312.0
72	5887.5
73	4628.5
74	3751.0
75	3035.5
76	2210.0
77	1437.5
78	926.5
79	613.5
80	387.5
81	248.5
82	145.0
83	95.0
84	59.5
85	37.0
86	26.5
87	14.5
88	9.0
89	11.5
90	8.5
91	5.5
92	6.0
93	4.0
94	5.0
95	3.5
96	0.5
97	2.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.47588164794339377
2	0.050343487690491115
3	0.05865099391004411
4	0.055798750107997584
5	0.06668158325561199
6	0.06845385124911663
7	0.04563590083274442
8	0.021987199794416913
9	0.013292009951284782
10-11	0.016435016471015664
12-13	0.011755121300667479
14-15	0.01647655400211343
16-17	0.01564580338015813
18-19	0.0587479148159389
20-21	0.08877954979962295
22-23	0.10446689071087883
24-25	0.07415833885320969
26-27	0.08966568379637527
28-29	0.09038566766873653
30-31	0.061337087587699575
32-33	0.035293055589400955
34-35	0.40302481839791404
36-37	0.6307197180321623
38-39	0.5771639946034439
40-41	0.5715564279052456
42-43	0.5445154951606007
44-45	0.5299081300578867
46-47	0.5199806601255209
48-49	0.5093747438518915
50-51	0.5240513381731019
52-53	0.6062264205281801
54-55	0.5757655643898192
56-57	0.5328849864532266
58-59	0.510648561472223
60-61	0.5236082711747257
62-63	0.5360695305040551
64-65	0.5541383565315829
66-67	0.5807085305904532
68-69	0.533397282670099
70-71	0.4824584237005399
72-73	0.4655526485437495
74-75	0.445268487524341
76-77	0.46047122390612294
78-79	0.4546975070835337
80-81	0.451568346407502
82-83	0.4709802192738575
84-85	0.43690559792999095
86-87	0.4399378377001278
88-89	0.4675187583490437
90-91	0.45000376606948617
92-93	0.4450054164940552
94-95	0.4564974667644368
96-97	0.45065452072335116
98	0.44539310011763433
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3611192.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.28351506025125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.99750215928145	45.64682578861489
2	12.880595762118876	15.272139856971656
3	4.324665102759958	7.691440462500389
4	1.9092655477032303	4.527518914051331
5	1.045648255940666	3.0994852064391956
6	0.6533742026064666	2.3240591628120066
7	0.42588050503105934	1.7673385333713347
8	0.29733145589688115	1.4101483074839347
9	0.21296809884001947	1.1362947745441834
>10	1.1677096769026312	12.17366650786624
>50	0.0638130284985085	2.5606350465286676
>100	0.020776700129860708	2.009930677336078
>500	3.286530046769791E-4	0.14108986010984717
>1k	1.4085128563293755E-4	0.23942690137026593
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4167	0.11539126138959102	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.538337479701993E-5
2	0.0	0.0	0.0	0.0	5.538337479701993E-5
3	0.0	0.0	0.0	0.0	5.538337479701993E-5
4	0.0	0.0	0.0	0.0	5.538337479701993E-5
5	0.0	2.7691687398509966E-5	0.0	0.0	1.1076674959403986E-4
6	0.0	2.7691687398509966E-5	0.0	0.0	1.1076674959403986E-4
7	0.0	2.7691687398509966E-5	0.0	0.0	1.1076674959403986E-4
8	0.0	2.7691687398509966E-5	0.0	0.0	1.1076674959403986E-4
9	0.0	2.7691687398509966E-5	0.0	0.0	1.1076674959403986E-4
10-11	0.0	2.7691687398509966E-5	0.0	0.0	1.1076674959403986E-4
12-13	0.0	2.7691687398509966E-5	0.0	1.3845843699254983E-5	2.7691687398509967E-4
14-15	0.0	2.7691687398509966E-5	0.0	4.153753109776495E-5	3.046085613836096E-4
16-17	0.0	2.7691687398509966E-5	0.0	5.538337479701993E-5	3.323002487821196E-4
18-19	0.0	2.7691687398509966E-5	0.0	5.538337479701993E-5	4.153753109776495E-4
20-21	0.0	2.7691687398509966E-5	0.0	5.538337479701993E-5	5.122962168724344E-4
22-23	0.0	2.7691687398509966E-5	0.0	5.538337479701993E-5	5.676795916694543E-4
24-25	0.0	2.7691687398509966E-5	0.0	1.3845843699254983E-4	6.369088101657293E-4
26-27	2.7691687398509966E-5	2.7691687398509966E-5	0.0	2.6307103028584466E-4	7.061380286620041E-4
28-29	2.7691687398509966E-5	2.7691687398509966E-5	0.0	7.199838723612592E-4	7.199838723612591E-4
30-31	2.7691687398509966E-5	2.7691687398509966E-5	0.0	0.0025199435532644067	7.476755597597691E-4
32-33	2.7691687398509966E-5	2.7691687398509966E-5	0.0	0.006064479540273683	8.30750621955299E-4
34-35	2.7691687398509966E-5	2.7691687398509966E-5	0.0	0.01067514549212559	8.584423093538089E-4
36-37	2.7691687398509966E-5	2.7691687398509966E-5	0.0	0.017445763061061277	8.584423093538089E-4
38-39	2.7691687398509966E-5	2.7691687398509966E-5	0.0	0.029159346830630994	8.999798404515739E-4
40-41	2.7691687398509966E-5	2.7691687398509966E-5	0.0	0.04243751093821652	9.138256841508288E-4
42-43	2.7691687398509966E-5	2.7691687398509966E-5	1.3845843699254983E-5	0.055369528953320674	9.276715278500839E-4
44-45	2.7691687398509966E-5	2.7691687398509966E-5	2.7691687398509966E-5	0.06783078828265016	0.0010245924337448687
46-47	2.7691687398509966E-5	2.7691687398509966E-5	2.7691687398509966E-5	0.0816904778256044	0.0011215133396396536
48-49	2.7691687398509966E-5	2.7691687398509966E-5	2.7691687398509966E-5	0.09575785502404746	0.0012184342455344385
50-51	2.7691687398509966E-5	2.7691687398509966E-5	2.7691687398509966E-5	0.10946524028630988	0.0012184342455344385
52-53	2.7691687398509966E-5	2.7691687398509966E-5	2.7691687398509966E-5	0.12394799279573061	0.0013568926825269882
54-55	2.7691687398509966E-5	2.7691687398509966E-5	2.7691687398509966E-5	0.13866612464803865	0.0014122760573240082
56-57	2.7691687398509966E-5	2.7691687398509966E-5	2.7691687398509966E-5	0.15391039856091837	0.0014122760573240082
58-59	2.7691687398509966E-5	4.153753109776495E-5	2.7691687398509966E-5	0.16979158128396385	0.0014122760573240082
60-61	2.7691687398509966E-5	5.538337479701993E-5	2.7691687398509966E-5	0.18563122647591157	0.0014676594321210283
62-63	2.7691687398509966E-5	5.538337479701993E-5	2.7691687398509966E-5	0.20109703388797937	0.001606117869113578
64-65	2.7691687398509966E-5	5.538337479701993E-5	2.7691687398509966E-5	0.21721359595391215	0.001689192931309108
66-67	2.7691687398509966E-5	5.538337479701993E-5	2.7691687398509966E-5	0.23443782551578538	0.001730730462406873
68-69	2.7691687398509966E-5	5.538337479701993E-5	2.7691687398509966E-5	0.2522712722004258	0.001744576306106128
70-71	2.7691687398509966E-5	5.538337479701993E-5	2.7691687398509966E-5	0.2711570030062096	0.0017584221498053829
72-73	2.7691687398509966E-5	8.30750621955299E-5	2.7691687398509966E-5	0.2896550501884142	0.0019938014926927177
74-75	2.7691687398509966E-5	1.2461259329329486E-4	2.7691687398509966E-5	0.3078761804966338	0.0020630307111889927
76-77	2.7691687398509966E-5	1.3845843699254983E-4	2.7691687398509966E-5	0.3284511042337267	0.0020768765548882474
78-79	2.7691687398509966E-5	1.3845843699254983E-4	2.7691687398509966E-5	0.34840296500435314	0.0021737974607830323
80-81	2.7691687398509966E-5	1.3845843699254983E-4	2.7691687398509966E-5	0.3678979129329042	0.002229180835580052
82-83	2.7691687398509966E-5	1.3845843699254983E-4	2.7691687398509966E-5	0.3874067067051544	0.002298410054076327
84-85	2.7691687398509966E-5	1.3845843699254983E-4	2.7691687398509966E-5	0.4078985553800518	0.002312255897775582
86	2.7691687398509966E-5	1.3845843699254983E-4	2.7691687398509966E-5	0.4246797179435488	0.002326101741474837
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6600	0.0	38.285515	1
GGTATCA	4070	0.0	29.967987	1
TCAACGC	8485	0.0	29.124224	4
TATCAAC	8770	0.0	28.59639	2
CAACGCA	8800	0.0	28.133812	5
ATCAACG	8925	0.0	27.842905	3
AACGCAG	9430	0.0	26.442123	6
GTACATG	12225	0.0	26.015686	1
TACATGG	12460	0.0	24.837574	2
GTACCTG	3725	0.0	23.970747	1
ACATGGG	12720	0.0	23.681091	3
ACGCAGA	11010	0.0	22.308598	7
TACCTGG	4050	0.0	21.056265	2
CGCAGAG	11730	0.0	20.895864	8
CATGGGG	10195	0.0	20.19187	4
GAGTACA	9180	0.0	20.055035	1
AGTACAT	9060	0.0	19.12877	2
GCAGAGT	13100	0.0	18.466015	9
GTATAGG	715	0.0	18.024286	1
GTGTAGG	1240	0.0	17.816633	1
>>END_MODULE