FastQCFastQC Report
Mon 15 Aug 2016
SRR2921477_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921477_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3611192
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41920.11608355357455379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72250.034.952891
TCAACGC87950.028.0336384
CAACGCA89650.027.5389945
TATCAAC91100.027.1149522
GGTATCA46350.026.7281321
ATCAACG92350.026.6461683
AACGCAG97950.025.448136
GTACATG121550.024.892231
TACATGG126250.023.5917422
ACATGGG128300.021.9938723
GTACCTG38900.021.680471
ACGCAGA114450.021.6136937
GTATAGG7550.021.4573941
GAGTACA92150.020.9413531
CGCAGAG120450.020.3793728
AGTACAT88750.020.285922
TACCTGG40100.019.4277862
AGAGTAC107200.018.02617610-11
GCAGAGT137700.017.791929
CATGGGG99000.017.1309324
Produced by FastQC (version 0.11.5)