##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921477_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3611192
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94350951154079	33.0	31.0	34.0	30.0	34.0
2	32.05622381750956	34.0	31.0	34.0	30.0	34.0
3	32.18390188059787	34.0	31.0	34.0	30.0	34.0
4	35.725939523570055	37.0	35.0	37.0	35.0	37.0
5	35.52781076165432	37.0	35.0	37.0	33.0	37.0
6	35.56552711680797	37.0	35.0	37.0	33.0	37.0
7	35.49616663971342	37.0	35.0	37.0	33.0	37.0
8	35.44843641656273	37.0	35.0	37.0	33.0	37.0
9	37.07282110726874	39.0	37.0	39.0	34.0	39.0
10-11	36.98807499019715	39.0	37.0	39.0	33.0	39.0
12-13	36.8654433217619	39.0	37.0	39.0	33.0	39.0
14-15	38.15851663384279	40.0	38.0	41.0	33.0	41.0
16-17	38.05934508605469	40.0	38.0	41.0	33.0	41.0
18-19	37.95133795156835	40.0	38.0	41.0	33.0	41.0
20-21	37.83331168766435	40.0	38.0	41.0	33.0	41.0
22-23	37.795511426697885	40.0	38.0	41.0	33.0	41.0
24-25	37.84483364495712	40.0	38.0	41.0	33.0	41.0
26-27	37.73261903548745	40.0	38.0	41.0	33.0	41.0
28-29	37.59281685936389	40.0	38.0	41.0	32.0	41.0
30-31	37.44974152578982	40.0	37.5	41.0	32.0	41.0
32-33	37.281906500679	40.0	37.0	41.0	31.5	41.0
34-35	37.12917992175437	40.0	37.0	41.0	31.0	41.0
36-37	37.02566119442001	40.0	37.0	41.0	31.0	41.0
38-39	36.9918420288924	40.0	37.0	41.0	31.0	41.0
40-41	36.8192060959373	40.0	36.5	41.0	30.5	41.0
42-43	36.569660654985945	39.0	36.0	41.0	30.0	41.0
44-45	36.3581012862235	39.0	35.5	41.0	30.0	41.0
46-47	36.07572748831964	39.0	35.0	40.0	29.5	41.0
48-49	35.735536770130196	39.0	35.0	40.0	28.5	41.0
50-51	35.446404677458304	38.5	35.0	40.0	28.0	41.0
52-53	35.127546112197855	38.0	34.0	40.0	27.0	41.0
54-55	34.786537658479524	38.0	34.0	40.0	26.0	41.0
56-57	34.38942792296837	37.5	34.0	40.0	26.0	41.0
58-59	33.98817869556645	37.0	33.0	40.0	25.0	41.0
60-61	33.54817910540342	36.5	33.0	39.0	23.5	40.5
62-63	33.09622210616328	36.0	32.0	39.0	22.5	40.0
64-65	32.56932503173468	35.0	31.5	38.5	21.5	40.0
66-67	32.03463163963589	35.0	31.0	38.0	20.0	40.0
68-69	31.519731573397372	35.0	31.0	37.0	18.0	39.5
70-71	30.952374728344545	34.0	30.0	36.5	16.5	39.0
72-73	30.395660767968028	34.0	29.5	36.0	12.5	39.0
74-75	29.869683749853234	34.0	29.0	35.5	8.0	38.0
76-77	28.613481227251278	32.5	27.5	34.5	7.5	36.0
78-79	29.553392204014628	34.0	29.0	35.0	7.0	37.0
80-81	29.58062684011263	34.0	29.0	35.0	7.0	36.0
82-83	29.23808994370834	34.0	29.0	35.0	2.0	36.0
84-85	28.734392688065327	34.0	29.0	35.0	2.0	35.5
86-87	28.197048232273445	33.0	27.5	35.0	2.0	35.0
88-89	27.639650979510364	33.0	26.5	35.0	2.0	35.0
90-91	27.141270250930994	33.0	25.5	34.5	2.0	35.0
92-93	26.677363180910902	32.0	25.0	34.0	2.0	35.0
94-95	26.148256863661636	32.0	24.5	34.0	2.0	35.0
96-97	25.348034527103515	32.0	21.5	34.0	2.0	35.0
98-99	24.2967151843491	31.5	10.0	34.0	2.0	35.0
100-101	20.711297266941223	27.0	2.0	32.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	22099.0
3	11940.0
4	7294.0
5	5528.0
6	4786.0
7	4911.0
8	5924.0
9	7107.0
10	8488.0
11	9904.0
12	11562.0
13	13822.0
14	15594.0
15	17471.0
16	20191.0
17	23218.0
18	23624.0
19	21508.0
20	21538.0
21	23022.0
22	24921.0
23	27405.0
24	31283.0
25	36177.0
26	43188.0
27	52088.0
28	63271.0
29	76932.0
30	94911.0
31	117715.0
32	151046.0
33	201174.0
34	284113.0
35	424637.0
36	639287.0
37	750639.0
38	304252.0
39	8622.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.335433517517295	17.349673305196923	13.61939951270097	24.695493664584816
2	16.45631137862512	18.825916761003015	38.12489061783478	26.592881242537093
3	20.681573472819476	21.983329677803848	27.191711474596286	30.14338537478039
4	14.408759403194168	14.658522981213979	33.79056822460878	37.14214939098308
5	16.285151666785282	35.14312385476963	32.12731707951959	16.444407398925502
6	36.10678993427809	32.38961417540585	16.061467916855324	15.442127973460739
7	32.131610751293	29.622284748143183	18.76936941780411	19.476735082759706
8	28.38266244120315	33.21187713038098	18.480091679843518	19.925368748572353
9	26.89578461375567	16.679107280406075	18.7517514102057	37.673356695632556
10-11	27.119625039257063	24.666949031095477	25.47688496966569	22.73654095998177
12-13	27.206860469145212	21.62070614323828	26.328859013894185	24.843574373722323
14-15	24.149616036971732	23.324468917879045	23.84443241832282	28.681482626826398
16-17	24.670320043269868	26.452916664646757	24.44331945253369	24.433443839549685
18-19	24.68249441309288	25.605750379732388	25.964442698569634	23.7473125086051
20-21	25.173458018222146	24.781758741111044	25.911290819608784	24.133492421058026
22-23	24.934124672978157	24.436487707497065	25.875002891546973	24.754384727977804
24-25	24.639172002135204	24.729200675575168	25.898617741299702	24.733009580989933
26-27	24.663617450027843	24.94725359309147	25.902110701049775	24.48701825583091
28-29	24.689346479860237	25.019302238782714	25.83678927079431	24.45456201056274
30-31	24.843755646666846	24.899445195294884	25.699660531908574	24.557138626129696
32-33	24.601038935431806	24.901675545083858	25.662433494950438	24.834852024533895
34-35	24.80477209610436	24.939890465223705	25.809920939553155	24.44541649911878
36-37	24.734598178214597	24.9685907424632	25.741896148455805	24.5549149308664
38-39	24.825870281616748	24.955087085977574	25.66876377974455	24.55027885266113
40-41	24.8725601407146	24.935572051904643	25.651082690046344	24.54078511733441
42-43	24.87310867646295	24.860840819011027	25.60225695345899	24.663793551067034
44-45	24.770817650613655	24.87765401469315	25.621645005818877	24.729883328874315
46-47	24.778856290380745	24.95863249870787	25.60829754243527	24.654213668476107
48-49	24.86340015978533	25.019973166034127	25.423064195209488	24.69356247897106
50-51	24.766011879796302	25.176655093901957	25.37764262602846	24.679690400273284
52-53	24.928145064031824	25.109752028124433	25.32031142908176	24.64179147876198
54-55	24.879757698747344	24.918167664624228	25.419006480677876	24.783068155950556
56-57	24.806639480784824	25.141983716440482	25.367761952808664	24.68361484996603
58-59	24.891609968407558	25.022745533727015	25.365780443534792	24.719864054330635
60-61	24.938825776142775	24.986610349825728	25.38140425444711	24.693159619584392
62-63	24.74795586085798	25.127792883646954	25.41594224272651	24.708309012768556
64-65	24.85576786605676	24.949843288402647	25.411303035988624	24.783085809551963
66-67	24.86082231186772	25.04810550466594	25.300794454168045	24.79027772929829
68-69	24.838800535500457	25.10746515513262	25.334926774621113	24.71880753474581
70-71	24.777767469040324	24.956968714212717	25.4176215711716	24.84764224557536
72-73	24.99503465492306	25.002056774738985	25.222207848652406	24.78070072168555
74-75	24.7730789162978	25.21779894133278	25.19495548990199	24.81416665246743
76-77	24.895933341786527	24.951846645889212	25.294131616806936	24.858088395517324
78-79	24.823102856334454	24.94302725200487	25.41245747741424	24.821412414246435
80-81	24.788683663388074	25.11111360423321	25.328306091126663	24.771896641252056
82-83	24.918386298620764	25.094514836305166	25.225492864211223	24.761606000862844
84-85	24.85033468611103	25.130173405281443	25.19654975906893	24.822942149538598
86-87	24.822037997110797	25.076080307090937	25.335612126770524	24.76626956902774
88-89	24.74169174386556	24.95095800490775	25.334984004368504	24.972366246858186
90-91	24.819531210248304	25.063211041901813	25.266462426291913	24.85079532155797
92-93	24.892767239664458	25.17101000211113	25.28652660054003	24.64969615768438
94-95	24.85244970938585	25.042333775283776	25.248003434058802	24.85721308127157
96-97	24.805433758859436	25.036811326340402	25.29495447359042	24.862800441209743
98-99	24.807808386238502	25.048310138697005	25.258652240254158	24.88522923481033
100-101	25.320225761515857	24.50969151649593	25.354164762703927	24.815917959284274
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	191.0
1	163.0
2	271.5
3	417.0
4	310.5
5	227.0
6	521.5
7	1280.0
8	1888.5
9	1760.0
10	1206.5
11	815.5
12	752.0
13	784.5
14	862.5
15	937.0
16	1029.0
17	1111.0
18	1202.0
19	1266.0
20	1318.5
21	1459.0
22	1554.0
23	1691.0
24	1899.0
25	2295.0
26	2891.0
27	3731.5
28	4990.0
29	6866.5
30	9561.5
31	13104.5
32	17492.0
33	22612.5
34	28091.0
35	34331.0
36	42138.0
37	50199.0
38	58556.0
39	67526.0
40	76667.0
41	85951.0
42	95861.0
43	106803.0
44	117807.0
45	129169.0
46	139054.0
47	147557.5
48	157775.5
49	164217.0
50	163878.0
51	163155.5
52	163745.5
53	164077.5
54	163100.5
55	158544.0
56	149449.0
57	137892.5
58	126911.5
59	114878.5
60	100285.0
61	86087.0
62	71403.5
63	54930.0
64	40934.5
65	31328.5
66	24440.0
67	19257.5
68	15150.0
69	11920.0
70	9395.0
71	7278.0
72	5686.5
73	4678.0
74	3720.0
75	2919.5
76	2238.5
77	1508.5
78	937.0
79	553.5
80	348.0
81	219.5
82	132.0
83	69.5
84	30.5
85	12.5
86	7.0
87	6.5
88	7.0
89	3.5
90	0.5
91	1.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3142729602856896
2	0.0
3	0.006202937977266232
4	0.06034018684135321
5	5.261420605716893E-4
6	0.06507546538649842
7	0.07568138166012774
8	0.10677914660865444
9	0.09373636184395623
10-11	0.09861009882609398
12-13	0.09877624895048503
14-15	0.06593390769585222
16-17	0.035708430900378604
18-19	0.03912835429409458
20-21	0.02837013373977346
22-23	0.04243751093821652
24-25	0.034088467187565766
26-27	0.020948761516972787
28-29	0.023801005319019315
30-31	0.0028107062709487613
32-33	0.005206037230919874
34-35	0.00786443922117683
36-37	0.010896678991313672
38-39	0.016435016471015664
40-41	0.02114260332876236
42-43	0.0035860735181070407
44-45	0.014676594321210283
46-47	0.02409176803670367
48-49	0.0026860936776554665
50-51	0.007518293128695456
52-53	0.0026860936776554665
54-55	0.004153753109776495
56-57	0.003502998455911511
58-59	0.0012045884018351835
60-61	0.005372187355310933
62-63	0.015631957536458876
64-65	0.007185992879913336
66-67	0.03926681273108713
68-69	0.06179400042977499
70-71	0.012378184267133956
72-73	0.03242696594365517
74-75	0.05102193403175461
76-77	0.3039301150423461
78-79	0.07389526782292384
80-81	0.03490537196582181
82-83	0.02892396748774366
84-85	0.01992416908322792
86-87	0.11942040190607423
88-89	0.5293958338410143
90-91	0.13348777910451728
92-93	0.04857121969698648
94-95	0.008432118812846286
96-97	0.005842946041085603
98-99	0.011284362614892811
100-101	0.00836288959435001
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3611192.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.11427511785971
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.94308415799132	39.47262124070737
2	14.731863527734882	15.944082318772173
3	5.148660279888075	8.358480566227795
4	2.3857183228286996	5.164056707010845
5	1.2472887589415051	3.3748063526387204
6	0.8064599692971747	2.6184597990052803
7	0.5226875655720642	1.9799401106835712
8	0.3700881704835906	1.602164246033151
9	0.28670365646050233	1.3963284488699963
>10	1.4436336464657455	13.95232208392172
>50	0.08377946705388233	3.1109015138827725
>100	0.029520503479164732	2.616854055403057
>500	3.0718428220634077E-4	0.12905031507523168
>1k	2.0478952119541352E-4	0.2799322417683023
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4192	0.11608355357455379	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.7691687398509966E-5
2	0.0	0.0	0.0	0.0	2.7691687398509966E-5
3	0.0	0.0	0.0	0.0	2.7691687398509966E-5
4	0.0	0.0	0.0	0.0	2.7691687398509966E-5
5	0.0	0.0	0.0	0.0	2.7691687398509966E-5
6	0.0	0.0	0.0	0.0	2.7691687398509966E-5
7	0.0	0.0	0.0	0.0	2.7691687398509966E-5
8	0.0	0.0	0.0	0.0	5.538337479701993E-5
9	0.0	0.0	0.0	0.0	5.538337479701993E-5
10-11	0.0	0.0	0.0	0.0	8.30750621955299E-5
12-13	0.0	0.0	0.0	0.0	1.799959680903148E-4
14-15	0.0	0.0	0.0	1.3845843699254983E-5	2.2153349918807976E-4
16-17	0.0	0.0	0.0	2.7691687398509966E-5	2.492251865865897E-4
18-19	0.0	0.0	0.0	4.153753109776495E-5	2.492251865865897E-4
20-21	0.0	0.0	0.0	5.538337479701993E-5	2.492251865865897E-4
22-23	0.0	0.0	0.0	5.538337479701993E-5	2.907627176843546E-4
24-25	0.0	0.0	0.0	1.1076674959403986E-4	3.323002487821196E-4
26-27	0.0	0.0	0.0	2.0768765548882475E-4	3.323002487821196E-4
28-29	0.0	0.0	0.0	6.507546538649842E-4	3.323002487821196E-4
30-31	0.0	0.0	0.0	0.002409176803670367	3.4614609248137453E-4
32-33	0.0	0.0	0.0	0.006009096165476662	4.153753109776495E-4
34-35	0.0	0.0	0.0	0.010592070429930062	4.2922115467690445E-4
36-37	8.30750621955299E-5	0.0	0.0	0.017445763061061277	4.4306699837615946E-4
38-39	8.30750621955299E-5	0.0	0.0	0.029034734237337698	4.707586857746694E-4
40-41	8.30750621955299E-5	0.0	0.0	0.04242366509451727	5.399879042709444E-4
42-43	8.30750621955299E-5	0.0	0.0	0.055438758171816954	5.676795916694543E-4
44-45	8.30750621955299E-5	0.0	0.0	0.0679415550322442	5.953712790679642E-4
46-47	8.30750621955299E-5	0.0	0.0	0.08163509445080738	6.507546538649842E-4
48-49	8.30750621955299E-5	0.0	0.0	0.09545324646266384	6.922921849627492E-4
50-51	8.30750621955299E-5	0.0	0.0	0.10899448160053524	6.922921849627492E-4
52-53	8.30750621955299E-5	0.0	0.0	0.12308955048637679	8.30750621955299E-4
54-55	8.30750621955299E-5	0.0	0.0	0.13777999065128632	9.138256841508289E-4
56-57	8.30750621955299E-5	0.0	0.0	0.15277503937757947	9.83054902647104E-4
58-59	8.30750621955299E-5	0.0	0.0	0.16821315510224877	0.0010384382774441237
60-61	8.30750621955299E-5	0.0	0.0	0.18374819173281287	0.0011076674959403987
62-63	8.30750621955299E-5	0.0	0.0	0.19897861980199336	0.0011353591833389086
64-65	1.1076674959403986E-4	0.0	0.0	0.21465211486955	0.0011492050270381635
66-67	1.1076674959403986E-4	0.0	0.0	0.2311978980901597	0.0011907425581359284
68-69	1.1076674959403986E-4	0.0	0.0	0.248643661151221	0.0012322800892336934
70-71	1.1076674959403986E-4	0.0	0.0	0.26694786652163605	0.0012738176203314585
72-73	1.1076674959403986E-4	0.0	0.0	0.28475362151887795	0.0015091969632187931
74-75	1.1076674959403986E-4	0.0	0.0	0.30240707223542806	0.001606117869113578
76-77	1.1076674959403986E-4	0.0	0.0	0.322331241318656	0.001619963712812833
78-79	1.1076674959403986E-4	0.0	0.0	0.34170157665391376	0.0017168846187076178
80-81	1.1076674959403986E-4	0.0	0.0	0.3606011533033968	0.001744576306106128
82-83	1.1076674959403986E-4	0.0	0.0	0.37961149670247385	0.0018276513683016576
84-85	1.1076674959403986E-4	0.0	0.0	0.399549511629401	0.0018276513683016576
86-87	1.1076674959403986E-4	0.0	0.0	0.42089980261365223	0.0019384181178956975
88-89	1.1076674959403986E-4	0.0	0.0	0.44209778931721155	0.0020768765548882474
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7225	0.0	34.95289	1
TCAACGC	8795	0.0	28.033638	4
CAACGCA	8965	0.0	27.538994	5
TATCAAC	9110	0.0	27.114952	2
GGTATCA	4635	0.0	26.728132	1
ATCAACG	9235	0.0	26.646168	3
AACGCAG	9795	0.0	25.44813	6
GTACATG	12155	0.0	24.89223	1
TACATGG	12625	0.0	23.591742	2
ACATGGG	12830	0.0	21.993872	3
GTACCTG	3890	0.0	21.68047	1
ACGCAGA	11445	0.0	21.613693	7
GTATAGG	755	0.0	21.457394	1
GAGTACA	9215	0.0	20.941353	1
CGCAGAG	12045	0.0	20.379372	8
AGTACAT	8875	0.0	20.28592	2
TACCTGG	4010	0.0	19.427786	2
AGAGTAC	10720	0.0	18.026176	10-11
GCAGAGT	13770	0.0	17.79192	9
CATGGGG	9900	0.0	17.130932	4
>>END_MODULE