FastQCFastQC Report
Mon 15 Aug 2016
SRR2921476_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921476_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4053748
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT80040.19744690592508463No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40780.10059826116472952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG41250.027.1176821
TACCTGG44900.024.8120352
GTACATG124000.024.7613531
TACATGG127150.023.436862
ACATGGG133250.021.9854873
GAGTACA96400.021.201971
ACCGCTC16650.021.1856238
AGTACAT91000.020.2025132
GTATCAA153400.018.9653991
GTATAGG9000.017.9017621
CATGGGG104200.017.4232794
ACCTGGG58900.017.2798563
AGAGTAC110450.017.27526510-11
TATCAAC172950.016.6737212
TCAACGC171350.016.5618534
CAACGCA173200.016.4114235
AACCGCT17700.016.3090447
ATCAACG176150.016.2406863
GTATTAC7700.016.1414781
AACGCAG178800.016.072546
Produced by FastQC (version 0.11.5)