##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921476_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4053748
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.164749264137782	31.0	31.0	34.0	27.0	34.0
2	30.255857788890676	31.0	31.0	34.0	26.0	34.0
3	30.39038687160623	31.0	31.0	34.0	26.0	34.0
4	33.887586006826275	37.0	35.0	37.0	30.0	37.0
5	33.86243545479393	37.0	35.0	37.0	30.0	37.0
6	33.96553288462923	37.0	35.0	37.0	30.0	37.0
7	33.83981145349933	37.0	35.0	37.0	30.0	37.0
8	33.81716475715807	37.0	35.0	37.0	30.0	37.0
9	35.245992844153115	39.0	35.0	39.0	29.0	39.0
10-11	35.10763606913898	39.0	35.0	39.0	29.0	39.0
12-13	34.938558958277625	39.0	35.0	39.0	28.0	39.0
14-15	35.91644510216224	39.5	36.0	41.0	27.0	41.0
16-17	35.76041122931174	39.0	36.0	41.0	27.0	41.0
18-19	35.56790826662141	39.0	36.0	40.5	27.0	41.0
20-21	35.444043759010185	39.0	36.0	40.0	27.0	41.0
22-23	35.48323243082698	39.0	36.0	41.0	27.0	41.0
24-25	35.34488539988179	39.0	36.0	41.0	26.0	41.0
26-27	35.06134088749474	39.0	35.0	40.0	24.5	41.0
28-29	34.81310481065917	39.0	34.5	40.0	24.0	41.0
30-31	34.55036425549886	38.5	34.0	40.0	23.5	41.0
32-33	34.264646445709005	38.0	34.0	40.0	21.5	41.0
34-35	33.83201175800765	38.0	33.5	40.0	17.5	41.0
36-37	33.433232406158446	38.0	33.0	40.0	15.5	41.0
38-39	33.18440502468333	38.0	33.0	40.0	14.0	41.0
40-41	32.908579911726136	38.0	32.0	40.0	9.0	41.0
42-43	32.60884495040146	38.0	31.5	40.0	9.0	41.0
44-45	32.2820386220357	37.0	31.0	40.0	8.0	41.0
46-47	31.9768145429859	37.0	31.0	40.0	7.0	41.0
48-49	31.746165893883884	37.0	30.0	40.0	4.0	41.0
50-51	31.023499734073255	36.0	29.5	38.5	2.0	39.5
52-53	31.564538545563266	36.5	30.5	39.0	2.0	40.0
54-55	31.984451796214266	37.0	31.0	40.0	2.0	40.5
56-57	31.818003610485903	37.0	31.0	40.0	2.0	41.0
58-59	31.47003661796441	36.0	31.0	39.0	2.0	41.0
60-61	30.99034806801015	36.0	30.0	39.0	2.0	40.0
62-63	30.4933657691598	35.0	29.0	39.0	2.0	40.0
64-65	29.932342982346213	35.0	28.5	38.0	2.0	40.0
66-67	29.421771407596133	34.5	28.0	38.0	2.0	40.0
68-69	28.88921338968283	34.0	27.5	37.0	2.0	39.0
70-71	28.34034972080159	34.0	26.0	36.0	2.0	39.0
72-73	27.763301887537164	33.5	26.0	36.0	2.0	39.0
74-75	27.149521134515513	33.0	25.0	35.0	2.0	37.5
76-77	26.540342048889077	32.5	23.5	35.0	2.0	37.0
78-79	25.94934095557987	32.0	21.5	35.0	2.0	36.5
80-81	25.333554897837754	32.0	19.5	34.5	2.0	36.0
82-83	24.676289448678112	31.0	17.0	34.0	2.0	35.5
84-85	24.02605675044428	31.0	9.0	34.0	2.0	35.0
86-87	23.37197699511662	30.5	4.5	34.0	2.0	35.0
88-89	22.685125099044143	30.0	2.0	34.0	2.0	35.0
90-91	21.90202388012279	29.0	2.0	34.0	2.0	35.0
92-93	21.04244269747404	29.0	2.0	34.0	2.0	35.0
94-95	20.094894403894862	27.5	2.0	33.0	2.0	34.5
96-97	19.16778028629308	26.5	2.0	33.0	2.0	34.0
98	18.54178528117683	25.0	2.0	32.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	141780.0
3	24621.0
4	18733.0
5	15678.0
6	15066.0
7	15813.0
8	17688.0
9	20385.0
10	23121.0
11	24924.0
12	27673.0
13	30361.0
14	26205.0
15	26416.0
16	29416.0
17	32997.0
18	36601.0
19	37239.0
20	39116.0
21	42288.0
22	46658.0
23	51230.0
24	56655.0
25	63512.0
26	71629.0
27	81440.0
28	93212.0
29	109165.0
30	128279.0
31	153839.0
32	189411.0
33	241786.0
34	319812.0
35	434741.0
36	566848.0
37	563449.0
38	226878.0
39	9083.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.11197946681638	16.914024109883762	13.149342722481036	24.824653700818825
2	16.106129297138487	18.53163223575469	38.883742947777705	26.478495519329115
3	20.95242984781086	21.730727554829112	26.955950600853136	30.36089199650689
4	13.927806099403725	14.343012599352631	34.32974336772901	37.39943793351463
5	15.928762489299118	35.45832851978787	32.315601712931006	16.297307277982004
6	35.91008605467895	32.60196359986561	16.289416837055473	15.198533508399963
7	31.961932534445026	29.721414109306004	18.841677764165723	19.474975592083247
8	28.06221583780398	33.42858968699874	18.552412666117764	19.956781809079523
9	26.79636762959631	16.91232725775377	18.818983716900707	37.472321395749205
10-11	27.00976902949953	24.75424457485169	25.6372321041964	22.598754291452376
12-13	27.208245271947007	21.732631136680524	26.326700064305935	24.732423527066533
14-15	24.046259004236763	23.42306811688459	24.07482978427334	28.455843094605303
16-17	24.55708607385972	26.50428514558935	24.56005908520811	24.378569695342822
18-19	24.52201248469834	26.014432647951423	25.812356324215134	23.651198543135102
20-21	25.09051235026946	25.011776975198728	25.701408990399667	24.196301684132145
22-23	24.76107222869405	24.75602235034179	25.665148616438284	24.817756804525875
24-25	24.60681796132623	24.96250795201709	25.793097217158138	24.63757686949854
26-27	24.669794247299272	24.98725989469167	25.954767194327594	24.388178663681465
28-29	24.512187293216	25.443937463581328	25.65049924446683	24.393375998735838
30-31	24.788036782293513	25.097180533459017	25.579266573142323	24.535516111105146
32-33	24.604977002284347	25.16590953565565	25.588774341068994	24.64033912099101
34-35	24.862624621724684	25.147003697893915	25.676629387216586	24.313742293164808
36-37	24.8205779115928	25.05713045553804	25.689248799829105	24.43304283304005
38-39	24.7378087927425	25.199931767077615	25.65406528650074	24.40819415367915
40-41	24.882941915744478	25.194075449851006	25.546729016597965	24.376253617806547
42-43	24.696443238820144	25.13477390637776	25.457072949736954	24.711709905065145
44-45	24.79296037370611	25.054240680690317	25.45128853934128	24.701510406262294
46-47	25.031167373912393	25.194285413173056	25.378431743296147	24.396115469618397
48-49	24.85553884105268	25.196927121158435	25.33822073441933	24.609313303369547
50-51	24.527870252152606	25.391317566516143	25.49129280519261	24.58951937613864
52-53	24.786968059231494	25.475639381238196	25.260882535724143	24.476510023806167
54-55	24.592739621939305	25.097461503868995	25.47350008528851	24.836298788903193
56-57	24.752569907510857	25.187561273157318	25.43880446878327	24.621064350548554
58-59	24.985777015086096	25.148258113610066	25.38702077776947	24.478944093534366
60-61	24.87247762104536	25.060027488963186	25.566112072391324	24.501382817600128
62-63	24.623821228806424	25.263494408191946	25.526945414241208	24.585738948760422
64-65	24.657482256449743	25.2718833812415	25.45150178948653	24.61913257282223
66-67	24.705155490987767	25.24636697647211	25.29748084315549	24.75099668938463
68-69	24.60845406877915	25.225441172750436	25.389165034346917	24.776939724123494
70-71	24.788898928041583	25.02200557482825	25.42306352800614	24.76603196912403
72-73	24.84838331088147	25.00165740235546	25.446733442736345	24.703225844026726
74-75	24.5386549361063	25.460508783238904	25.35005779009058	24.650778490564214
76-77	24.749584644330643	25.0914476384205	25.324787811739796	24.834179905509064
78-79	24.755326272112573	24.975343053704563	25.46418250204318	24.80514817213968
80-81	24.782657124523432	25.092197339042	25.374652939665616	24.750492596768954
82-83	24.899413103169742	25.030191249872512	25.36899005141776	24.701405595539985
84-85	24.826996863768425	25.143072912304937	25.324401011483	24.70552921244364
86-87	24.69763467097662	25.147778863305074	25.393831259172302	24.760755206546005
88-89	24.696500917204496	25.001421998458667	25.297110932857013	25.004966151479824
90-91	24.84565958627799	24.849760628016927	25.281906352526047	25.022673433179037
92-93	24.9539398280891	25.115755954866913	25.330201658748486	24.600102558295493
94-95	24.933412831523313	24.86971267649193	25.355548715935722	24.84132577604904
96-97	24.726253055829545	24.940648978837817	25.509249169469793	24.823848795862844
98	24.940066679303772	25.044384727936798	25.11639143799085	24.899157154768588
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	511.0
1	373.0
2	377.0
3	528.0
4	432.5
5	392.0
6	664.5
7	1321.5
8	1937.0
9	1931.5
10	1627.5
11	1486.0
12	1608.0
13	1873.5
14	2160.0
15	2506.0
16	2823.0
17	2916.0
18	2958.5
19	2937.5
20	2717.0
21	2495.0
22	2423.5
23	2470.5
24	2671.0
25	3089.0
26	3714.0
27	4750.5
28	6239.0
29	8428.5
30	11844.5
31	16257.5
32	22120.5
33	28870.5
34	36699.5
35	45835.5
36	55659.5
37	66119.0
38	77125.5
39	88207.5
40	99020.5
41	110633.0
42	122286.5
43	134317.0
44	145946.0
45	157494.0
46	174765.0
47	192213.0
48	197279.0
49	192472.5
50	189127.0
51	187867.0
52	186222.5
53	185543.5
54	182753.5
55	175534.5
56	163979.0
57	152250.0
58	140243.5
59	125625.5
60	109835.5
61	92404.0
62	74300.0
63	57158.0
64	42420.0
65	31890.0
66	24444.0
67	18633.5
68	14333.0
69	10946.5
70	8186.5
71	6231.5
72	4875.5
73	3699.0
74	2889.0
75	2198.0
76	1548.0
77	965.0
78	567.5
79	337.0
80	194.0
81	115.0
82	74.5
83	50.5
84	35.5
85	24.5
86	19.0
87	19.0
88	12.5
89	7.5
90	5.5
91	7.0
92	6.5
93	3.5
94	5.5
95	5.5
96	4.0
97	3.5
98	1.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.47617661482657525
2	0.04960841177103263
3	0.05777369486213746
4	0.055997560775854834
5	0.06611165765607531
6	0.06707373028614506
7	0.04400865569344715
8	0.020351536405321693
9	0.013000314770429736
10-11	0.015615178841901372
12-13	0.011532537296348958
14-15	0.016182555008352764
16-17	0.015257485171747232
18-19	0.05694729914143652
20-21	0.08564913260518414
22-23	0.10226338687061949
24-25	0.07126738021209014
26-27	0.08747460374941905
28-29	0.08949742312546316
30-31	0.05911812969133749
32-33	0.034264586747868885
34-35	0.39912446456957856
36-37	0.6282457617000365
38-39	0.5781193108205048
40-41	0.5714094709389927
42-43	0.5448908022896342
44-45	0.5300896849039457
46-47	0.5201482677265582
48-49	0.5095778030602791
50-51	0.5241939064786465
52-53	0.6053657010746598
54-55	0.5750357446984864
56-57	0.53281555735581
58-59	0.509738148498624
60-61	0.5234785191383382
62-63	0.5350357249636633
64-65	0.5531670937611316
66-67	0.5804628210732389
68-69	0.5341723264494981
70-71	0.48209706178085077
72-73	0.46424938106660796
74-75	0.4445638949436423
76-77	0.4601174024631033
78-79	0.4532595514077343
80-81	0.4501143139632755
82-83	0.46999714831805034
84-85	0.4364849517039539
86-87	0.4395685178259724
88-89	0.4670615933698888
90-91	0.4486712050181708
92-93	0.4438978446612863
94-95	0.45478900020425544
96-97	0.4495469377968241
98	0.4443542124473451
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4053748.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.49648833047756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.47939289310486	46.09751795117099
2	12.837758675491063	15.27603118451682
3	4.190018310597449	7.478741265628452
4	1.848351820925871	4.3988177017733205
5	0.9926181676390959	2.952864761377975
6	0.622285872361268	2.2214294485897934
7	0.3948491917972451	1.6444498232444298
8	0.288155518627058	1.3715393161085971
9	0.186988544051293	1.001264555617267
>10	1.0736653268970744	11.366360339781254
>50	0.061191960048837694	2.4836395730539444
>100	0.023099200780684396	2.2949945311068434
>500	9.163946114173514E-4	0.37060267613338427
>1k	6.664687688265232E-4	0.8433849838831475
>5k	4.165429805165686E-5	0.1983618880138887
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	8004	0.19744690592508463	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4078	0.10059826116472952	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4668528976147507E-5
2	0.0	0.0	0.0	0.0	2.4668528976147507E-5
3	0.0	0.0	0.0	0.0	2.4668528976147507E-5
4	0.0	0.0	0.0	0.0	2.4668528976147507E-5
5	0.0	4.9337057952295014E-5	0.0	0.0	2.4668528976147507E-5
6	0.0	4.9337057952295014E-5	0.0	0.0	2.4668528976147507E-5
7	0.0	4.9337057952295014E-5	0.0	2.4668528976147507E-5	2.4668528976147507E-5
8	2.4668528976147507E-5	4.9337057952295014E-5	0.0	2.4668528976147507E-5	2.4668528976147507E-5
9	2.4668528976147507E-5	4.9337057952295014E-5	0.0	4.9337057952295014E-5	2.4668528976147507E-5
10-11	2.4668528976147507E-5	4.9337057952295014E-5	0.0	9.867411590459003E-5	2.4668528976147507E-5
12-13	2.4668528976147507E-5	4.9337057952295014E-5	0.0	9.867411590459003E-5	2.4668528976147507E-5
14-15	2.4668528976147507E-5	4.9337057952295014E-5	0.0	1.1100838039266378E-4	4.9337057952295014E-5
16-17	2.4668528976147507E-5	4.9337057952295014E-5	0.0	1.7267970283303254E-4	9.867411590459003E-5
18-19	2.4668528976147507E-5	4.9337057952295014E-5	0.0	1.8501396732110628E-4	9.867411590459003E-5
20-21	2.4668528976147507E-5	4.9337057952295014E-5	0.0	2.590195542495488E-4	1.2334264488073754E-4
22-23	2.4668528976147507E-5	7.400558692844251E-5	0.0	3.330251411779913E-4	1.7267970283303254E-4
24-25	2.4668528976147507E-5	8.633985141651627E-5	0.0	4.193649925945076E-4	1.9734823180918005E-4
26-27	2.4668528976147507E-5	9.867411590459003E-5	0.0	5.673761664513927E-4	1.9734823180918005E-4
28-29	2.4668528976147507E-5	9.867411590459003E-5	0.0	9.620726300697527E-4	1.9734823180918005E-4
30-31	2.4668528976147507E-5	9.867411590459003E-5	0.0	0.0029108864191854057	2.096824962972538E-4
32-33	2.4668528976147507E-5	1.1100838039266378E-4	0.0	0.00716620766757085	3.206908766899176E-4
34-35	2.4668528976147507E-5	1.4801117385688503E-4	0.0	0.012297261694609532	3.453594056660651E-4
36-37	2.4668528976147507E-5	1.8501396732110628E-4	0.0	0.021116260803582265	3.453594056660651E-4
38-39	2.4668528976147507E-5	2.2201676078532754E-4	0.0	0.03432625807030926	3.7002793464221257E-4
40-41	2.4668528976147507E-5	2.2201676078532754E-4	0.0	0.048103631503487634	3.7002793464221257E-4
42-43	2.4668528976147507E-5	2.7135381873762257E-4	0.0	0.06260872654146236	3.7002793464221257E-4
44-45	2.4668528976147507E-5	3.453594056660651E-4	0.0	0.078433587879661	3.7002793464221257E-4
46-47	2.4668528976147507E-5	3.453594056660651E-4	0.0	0.09390075554770548	3.7002793464221257E-4
48-49	2.4668528976147507E-5	3.7002793464221257E-4	0.0	0.10934325468677382	3.7002793464221257E-4
50-51	2.4668528976147507E-5	3.946964636183601E-4	0.0	0.1246254083874972	3.7002793464221257E-4
52-53	2.4668528976147507E-5	4.193649925945076E-4	0.0	0.14000623620412517	4.193649925945076E-4
54-55	2.4668528976147507E-5	4.5636778605872885E-4	0.0	0.15649714782467977	4.687020505468026E-4
56-57	2.4668528976147507E-5	4.687020505468026E-4	0.0	0.17573860042607484	4.687020505468026E-4
58-59	2.4668528976147507E-5	5.180391084990976E-4	0.0	0.19395630907495975	4.687020505468026E-4
60-61	2.4668528976147507E-5	5.427076374752451E-4	0.0	0.21291407359312914	4.687020505468026E-4
62-63	2.4668528976147507E-5	5.427076374752451E-4	0.0	0.23204451781413152	5.180391084990976E-4
64-65	2.4668528976147507E-5	5.427076374752451E-4	0.0	0.24974418735451734	5.180391084990976E-4
66-67	2.4668528976147507E-5	5.427076374752451E-4	0.0	0.2695776846513399	5.180391084990976E-4
68-69	2.4668528976147507E-5	5.55041901963319E-4	0.0	0.2900032266435901	5.180391084990976E-4
70-71	2.4668528976147507E-5	5.673761664513927E-4	0.0	0.3114895153818146	5.920446954275401E-4
72-73	2.4668528976147507E-5	5.673761664513927E-4	0.0	0.33080497357013805	9.49738365581679E-4
74-75	2.4668528976147507E-5	5.673761664513927E-4	0.0	0.3522172567214341	9.620726300697527E-4
76-77	2.4668528976147507E-5	5.673761664513927E-4	0.0	0.37276614135856495	9.620726300697527E-4
78-79	2.4668528976147507E-5	5.673761664513927E-4	0.0	0.3959052215381913	9.620726300697527E-4
80-81	3.700279346422126E-5	5.673761664513927E-4	0.0	0.41869894231215166	9.867411590459003E-4
82-83	4.9337057952295014E-5	5.920446954275401E-4	0.0	0.440012551347543	0.0010114096880220478
84-85	4.9337057952295014E-5	6.167132244036877E-4	0.0	0.46239924139339694	0.0010114096880220478
86	4.9337057952295014E-5	6.167132244036877E-4	0.0	0.47906283271678457	0.0010360782169981952
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	4125	0.0	27.117682	1
TACCTGG	4490	0.0	24.812035	2
GTACATG	12400	0.0	24.761353	1
TACATGG	12715	0.0	23.43686	2
ACATGGG	13325	0.0	21.985487	3
GAGTACA	9640	0.0	21.20197	1
ACCGCTC	1665	0.0	21.185623	8
AGTACAT	9100	0.0	20.202513	2
GTATCAA	15340	0.0	18.965399	1
GTATAGG	900	0.0	17.901762	1
CATGGGG	10420	0.0	17.423279	4
ACCTGGG	5890	0.0	17.279856	3
AGAGTAC	11045	0.0	17.275265	10-11
TATCAAC	17295	0.0	16.673721	2
TCAACGC	17135	0.0	16.561853	4
CAACGCA	17320	0.0	16.411423	5
AACCGCT	1770	0.0	16.309044	7
ATCAACG	17615	0.0	16.240686	3
GTATTAC	770	0.0	16.141478	1
AACGCAG	17880	0.0	16.07254	6
>>END_MODULE