FastQCFastQC Report
Mon 15 Aug 2016
SRR2921476_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921476_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4053748
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT81270.20048113498915077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG40350.029.1629221
TACCTGG44050.025.3401852
GTACATG122150.024.259091
TACATGG125350.022.7360362
ACATGGG127650.021.1733723
TATCACG3750.020.2663972
AGTACAT89350.019.0316452
GAGTACA96900.018.9284291
ACCTGGG58150.018.216033
AGAGTAC111500.017.9486110-11
CATGGGG101950.017.7981434
GTATCAA163550.017.7104911
GTATAGG10250.017.6618961
GTATATA9050.017.3717251
GTATAGC10650.016.9985391
TACACTA7650.016.13625
GTATAAG10450.015.9561961
TCAACGC180900.015.7809994
TAGACTG14450.015.7711295
CAACGCA182200.015.6347975
Produced by FastQC (version 0.11.5)