##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921476_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4053748
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.913771156963875	33.0	31.0	34.0	30.0	34.0
2	32.02990294413959	34.0	31.0	34.0	30.0	34.0
3	32.15653957769452	34.0	31.0	34.0	30.0	34.0
4	35.69818720847966	37.0	35.0	37.0	35.0	37.0
5	35.49729805602124	37.0	35.0	37.0	33.0	37.0
6	35.53526785582133	37.0	35.0	37.0	33.0	37.0
7	35.46730581180675	37.0	35.0	37.0	33.0	37.0
8	35.41933119670981	37.0	35.0	37.0	33.0	37.0
9	37.03889807654546	39.0	37.0	39.0	34.0	39.0
10-11	36.952371854392524	39.0	37.0	39.0	33.0	39.0
12-13	36.83228385188226	39.0	37.0	39.0	33.0	39.0
14-15	38.1156021538586	40.0	38.0	41.0	33.0	41.0
16-17	38.01771225049016	40.0	38.0	41.0	33.0	41.0
18-19	37.915219569642716	40.0	38.0	41.0	33.0	41.0
20-21	37.798506221896375	40.0	38.0	41.0	33.0	41.0
22-23	37.76354277572261	40.0	38.0	41.0	32.5	41.0
24-25	37.81546830242038	40.0	38.0	41.0	33.0	41.0
26-27	37.704715734673194	40.0	38.0	41.0	32.5	41.0
28-29	37.57042852688426	40.0	38.0	41.0	32.0	41.0
30-31	37.437415818644865	40.0	37.5	41.0	32.0	41.0
32-33	37.280127057725345	40.0	37.0	41.0	31.5	41.0
34-35	37.1278576023966	40.0	37.0	41.0	31.0	41.0
36-37	37.027107753121314	40.0	37.0	41.0	31.0	41.0
38-39	36.99629367686398	40.0	37.0	41.0	31.0	41.0
40-41	36.83832751813877	40.0	36.5	41.0	30.5	41.0
42-43	36.59790593791227	39.5	36.0	41.0	30.0	41.0
44-45	36.39332600349109	39.0	36.0	41.0	30.0	41.0
46-47	36.124969287681424	39.0	35.0	40.0	29.5	41.0
48-49	35.79155364368974	39.0	35.0	40.0	28.5	41.0
50-51	35.51421462310928	38.5	35.0	40.0	28.0	41.0
52-53	35.2021870871105	38.0	34.5	40.0	27.0	41.0
54-55	34.8753589270966	38.0	34.0	40.0	26.5	41.0
56-57	34.47901904607785	38.0	34.0	40.0	26.0	41.0
58-59	34.07634206665042	37.0	33.0	40.0	25.0	41.0
60-61	33.628264756467345	36.5	33.0	39.0	23.5	41.0
62-63	33.18772627208203	36.0	32.0	39.0	22.5	40.0
64-65	32.662340505625906	35.5	31.5	39.0	22.0	40.0
66-67	32.11610391173797	35.0	31.0	38.0	20.0	40.0
68-69	31.59173917569617	35.0	31.0	37.5	18.5	39.5
70-71	31.017591374698178	34.5	30.0	37.0	17.0	39.0
72-73	30.44774502509776	34.0	29.5	36.0	12.5	39.0
74-75	29.91682475082319	34.0	29.0	35.5	8.0	38.0
76-77	28.65913643373984	32.5	27.5	34.5	7.5	36.0
78-79	29.594457154218762	34.0	29.0	35.0	7.5	37.0
80-81	29.606922285253056	34.0	29.0	35.0	7.0	36.5
82-83	29.255447427911157	34.0	29.0	35.0	2.0	36.0
84-85	28.745938573389367	34.0	29.0	35.0	2.0	35.5
86-87	28.212668991757752	33.0	27.5	35.0	2.0	35.0
88-89	27.646784654596193	33.0	26.5	35.0	2.0	35.0
90-91	27.137079808611688	32.5	25.5	34.5	2.0	35.0
92-93	26.67494020348576	32.0	25.0	34.0	2.0	35.0
94-95	26.146049162404765	32.0	24.5	34.0	2.0	35.0
96-97	25.34097494466849	32.0	21.5	34.0	2.0	35.0
98-99	24.28638891712065	31.0	10.0	34.0	2.0	35.0
100-101	20.690888037440907	27.0	2.0	32.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26249.0
3	13690.0
4	8075.0
5	6026.0
6	5432.0
7	5644.0
8	6573.0
9	7781.0
10	9288.0
11	11017.0
12	13011.0
13	15310.0
14	17092.0
15	19139.0
16	21777.0
17	24842.0
18	25541.0
19	24083.0
20	24709.0
21	26145.0
22	27876.0
23	30902.0
24	35371.0
25	40992.0
26	48675.0
27	58885.0
28	70932.0
29	87363.0
30	106304.0
31	132216.0
32	169899.0
33	224955.0
34	315193.0
35	467112.0
36	710031.0
37	851178.0
38	354515.0
39	9925.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.33570313391258	17.37355255040415	13.539786195798204	24.750958119885066
2	16.204633341786415	18.855118769099608	38.50542756974533	26.434820319368647
3	20.93261924856303	21.906200759987634	27.158641436373177	30.002538555076157
4	14.155143730222594	14.678106503033565	34.33686138413463	36.82988838260921
5	15.998755715101186	35.30739806805281	32.45824713708399	16.235599079762018
6	35.87856222324742	32.75911223932898	16.144121274863146	15.218204262560453
7	32.03018577647476	29.675010899130093	18.853841060812456	19.440962263582687
8	28.061006654746123	33.510371788163305	18.51268436738426	19.915937189706316
9	26.595160242553295	16.9413238605963	18.9035078081193	37.56000808873111
10-11	26.963357540070998	24.833134995372113	25.725316306763208	22.47819115779368
12-13	27.163810606956616	21.69641576255572	26.464439976325497	24.675333654162163
14-15	23.939556370030065	23.492374319220495	24.158469707279163	28.409599603470276
16-17	24.470252912706712	26.523451260553454	24.68371259442969	24.322583232310148
18-19	24.420029090471168	26.070743050787897	25.92774615972712	23.581481699013818
20-21	25.027401958728035	24.968563624115696	25.881304239469184	24.12273017768708
22-23	24.6517460696651	24.72131541591561	25.921281754124802	24.705656760294488
24-25	24.540926138054555	25.012128621127538	25.897283534341888	24.549661706476016
26-27	24.5825033158548	24.994383618647152	26.094417138049973	24.328695927448074
28-29	24.406347606816716	25.424897505324445	25.864855691962894	24.303899195895944
30-31	24.695599467439376	25.066995623421317	25.689130439609208	24.548274469530096
32-33	24.46910116413449	25.14752278047276	25.794564313320762	24.588811742071996
34-35	24.76452617947843	25.10333858568425	25.839087112299904	24.29304812253742
36-37	24.629976076395664	25.145861946715765	25.87437603603368	24.34978594085489
38-39	24.593980282981104	25.212408534460373	25.881945845122157	24.31166533743637
40-41	24.73818558157535	25.197163476146876	25.699690235567285	24.364960706710484
42-43	24.57637540385214	25.176468875894464	25.61436158829451	24.63279413195889
44-45	24.649714607858737	25.02094027057667	25.648543926458018	24.68080119510658
46-47	24.800723354791014	25.23922450326109	25.63712392200619	24.3229282199417
48-49	24.72752022591001	25.223702739963088	25.54965593349894	24.499121100627967
50-51	24.465639354146514	25.384884478531948	25.637864072161044	24.51161209516049
52-53	24.662270714554165	25.50390653764561	25.384212044764066	24.44961070303616
54-55	24.60167789229197	25.09697867141471	25.549638315101586	24.75170512119173
56-57	24.676202359771978	25.249037155011383	25.499085753643914	24.57567473157273
58-59	24.9557873381449	25.172058732472507	25.43645896110297	24.43569496827962
60-61	24.83202328435061	25.163166103290507	25.636209651913962	24.368600960444926
62-63	24.532925324427403	25.28123908649987	25.653564072353568	24.53227151671916
64-65	24.63357035233371	25.283679610506795	25.51005341716269	24.572696619996805
66-67	24.67520865738434	25.33273761005395	25.312193300605568	24.679860431956136
68-69	24.583378020585712	25.292655735948756	25.433462234201354	24.690504009264178
70-71	24.796167234707163	25.092163434310866	25.466195556343944	24.64547377463803
72-73	24.873176487890632	25.041122946381993	25.504641353126306	24.581059212601065
74-75	24.48727544217414	25.519221810530734	25.33895235656528	24.654550390729845
76-77	24.658389073596943	25.12954925543973	25.443429230587654	24.768632440375672
78-79	24.630668770118707	25.068656165946418	25.589627139221705	24.71104792471317
80-81	24.72801118640634	25.222740444995996	25.4104925244024	24.638755844195263
82-83	24.76035620823468	25.201859681473472	25.472770210057444	24.565013900234405
84-85	24.696713487834675	25.28660123570972	25.409412272394015	24.607273004061586
86-87	24.62432062583305	25.311468065617042	25.47952881741786	24.58468249113205
88-89	24.60518374255659	25.182443198978767	25.356157888811687	24.856215169652955
90-91	24.703946836218766	25.071406596639395	25.361664361886792	24.862982205255047
92-93	24.86814567280876	25.326902478392427	25.408347359951822	24.396604488846986
94-95	24.7348751505056	25.181189565701363	25.396254842052535	24.6876804417405
96-97	24.507275853855564	25.189018259604858	25.605126716706422	24.69857916983316
98-99	24.69267944641223	25.304606742931323	25.330252253634715	24.672461557021727
100-101	25.100411737154815	24.579705289500723	25.506314335086866	24.813568638257596
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	153.0
1	126.5
2	232.0
3	361.0
4	265.5
5	200.5
6	428.0
7	1061.5
8	1615.5
9	1505.5
10	1061.5
11	765.5
12	668.5
13	677.5
14	734.0
15	817.0
16	925.0
17	991.5
18	1052.0
19	1111.5
20	1185.0
21	1291.0
22	1445.0
23	1739.0
24	2036.5
25	2448.5
26	3034.5
27	3845.5
28	5108.0
29	6900.5
30	9751.5
31	13724.5
32	18470.0
33	24205.5
34	31225.0
35	39365.5
36	48505.0
37	58775.5
38	69573.5
39	80148.5
40	90960.0
41	101285.0
42	112875.0
43	125722.0
44	137811.5
45	150396.5
46	160610.5
47	174608.0
48	196113.5
49	201144.5
50	190326.5
51	185077.5
52	183063.5
53	181031.5
54	179030.5
55	173260.5
56	162485.0
57	149408.5
58	136883.5
59	123743.5
60	107846.5
61	90962.5
62	74003.0
63	56911.5
64	41847.5
65	30877.5
66	23427.0
67	18279.0
68	14076.5
69	10618.5
70	8246.0
71	6359.0
72	4736.5
73	3615.0
74	2801.5
75	2116.0
76	1548.0
77	959.0
78	553.5
79	316.0
80	168.5
81	79.0
82	43.5
83	27.5
84	15.0
85	7.5
86	5.5
87	5.5
88	3.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3147950982646183
2	0.0
3	0.0064384860627744985
4	0.0594018177745632
5	3.453594056660651E-4
6	0.062189361548867866
7	0.0731668569432535
8	0.10254707495384519
9	0.09050883281348521
10-11	0.0947888225908468
12-13	0.09425844921785961
14-15	0.06417517813144774
16-17	0.03602838656966343
18-19	0.03853224226074241
20-21	0.027579415395332915
22-23	0.04134445456402322
24-25	0.03330251411779913
26-27	0.02149862300271255
28-29	0.02408881854520804
30-31	0.0030835661220184386
32-33	0.0054640791682166725
34-35	0.00801727191724794
36-37	0.01058279893076728
38-39	0.01604687809898395
40-41	0.020462544785714357
42-43	0.0031945745024111022
44-45	0.01364169652380957
46-47	0.023805130461982342
48-49	0.0024298501041505292
50-51	0.0063891490048222045
52-53	0.002331175988245939
54-55	0.0037126136109102
56-57	0.0033549199407560608
58-59	0.001110083803926638
60-61	0.005180391084990976
62-63	0.014024058722939856
64-65	0.006796179732928638
66-67	0.037767517862481836
68-69	0.06027755055321642
70-71	0.01200123934689576
72-73	0.031230357683802744
74-75	0.050212790730948254
76-77	0.30181945202316474
78-79	0.07268582062821863
80-81	0.03279680927378811
82-83	0.027591749659820985
84-85	0.019081107163050098
86-87	0.11389459828287304
88-89	0.5176937490934316
90-91	0.13018816167161845
92-93	0.04756092386601239
94-95	0.008078943239688309
96-97	0.005858775631835033
98-99	0.010040091293292036
100-101	0.007708915305046095
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4053748.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.21337607347161
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.95595615745455	40.833580201947164
2	14.602647763028342	16.125229652170457
3	4.878466412133031	8.080698020247025
4	2.224116574018359	4.912039393300677
5	1.1736651209798659	3.240100685448895
6	0.7196140846853486	2.3839393851299517
7	0.4634658158925755	1.7912658673052588
8	0.3306340728316919	1.4604338724767847
9	0.24836052029881175	1.2341540528155237
>10	1.2913551263714347	12.707097748283294
>50	0.07988846491428182	2.992255865316864
>100	0.029952740544545043	2.74666323079368
>500	0.0011173493616541583	0.4385358825786084
>1k	7.151035157697885E-4	0.8534557601492428
>5k	4.4693969735611765E-5	0.2005503820365157
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	8127	0.20048113498915077	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.4668528976147507E-5	0.0
2	0.0	0.0	0.0	2.4668528976147507E-5	0.0
3	0.0	0.0	0.0	2.4668528976147507E-5	0.0
4	0.0	0.0	0.0	2.4668528976147507E-5	0.0
5	0.0	0.0	0.0	2.4668528976147507E-5	0.0
6	0.0	0.0	0.0	2.4668528976147507E-5	0.0
7	0.0	0.0	0.0	2.4668528976147507E-5	0.0
8	0.0	0.0	0.0	2.4668528976147507E-5	0.0
9	0.0	0.0	0.0	2.4668528976147507E-5	0.0
10-11	0.0	2.4668528976147507E-5	0.0	6.167132244036877E-5	0.0
12-13	0.0	4.9337057952295014E-5	0.0	1.3567690936881128E-4	6.167132244036877E-5
14-15	0.0	8.633985141651627E-5	0.0	2.590195542495488E-4	9.867411590459003E-5
16-17	0.0	9.867411590459003E-5	0.0	3.8236219913028634E-4	2.2201676078532754E-4
18-19	0.0	9.867411590459003E-5	0.0	4.687020505468026E-4	2.2201676078532754E-4
20-21	0.0	9.867411590459003E-5	0.0	5.550419019633189E-4	2.343510252734013E-4
22-23	0.0	9.867411590459003E-5	0.0	6.290474888917614E-4	3.206908766899176E-4
24-25	0.0	1.2334264488073754E-4	0.0	7.52390133772499E-4	3.453594056660651E-4
26-27	0.0	1.4801117385688503E-4	0.0	9.250698366055315E-4	3.453594056660651E-4
28-29	0.0	1.4801117385688503E-4	0.0	0.0013074320357358178	3.453594056660651E-4
30-31	0.0	1.4801117385688503E-4	0.0	0.0032685800893395445	3.453594056660651E-4
32-33	2.4668528976147507E-5	1.4801117385688503E-4	0.0	0.007474564279772694	3.7002793464221257E-4
34-35	2.4668528976147507E-5	1.4801117385688503E-4	0.0	0.012482275661930638	3.7002793464221257E-4
36-37	2.4668528976147507E-5	1.603454383449588E-4	0.0	0.021128595068070338	3.7002793464221257E-4
38-39	2.4668528976147507E-5	2.0968249629725383E-4	0.0	0.03416591263196429	3.7002793464221257E-4
40-41	2.4668528976147507E-5	2.590195542495488E-4	0.0	0.04783227768475001	3.946964636183601E-4
42-43	2.4668528976147507E-5	2.7135381873762257E-4	0.0	0.06249771816106971	4.070307281064338E-4
44-45	2.4668528976147507E-5	2.7135381873762257E-4	0.0	0.07840891935068485	4.440335215706551E-4
46-47	2.4668528976147507E-5	2.8368808322569634E-4	0.0	0.09386375275424126	4.687020505468026E-4
48-49	2.4668528976147507E-5	2.9602234771377006E-4	0.0	0.10912123792598849	4.687020505468026E-4
50-51	2.4668528976147507E-5	2.9602234771377006E-4	0.0	0.12404569795655773	4.687020505468026E-4
52-53	2.4668528976147507E-5	3.453594056660651E-4	0.0	0.1392908488638169	5.303733729871713E-4
54-55	2.4668528976147507E-5	3.453594056660651E-4	0.0	0.15560908078153846	5.920446954275401E-4
56-57	2.4668528976147507E-5	3.453594056660651E-4	0.0	0.17439416559687482	6.043789599156138E-4
58-59	2.4668528976147507E-5	3.7002793464221257E-4	0.0	0.19189648690545147	6.167132244036877E-4
60-61	2.4668528976147507E-5	3.7002793464221257E-4	0.0	0.21036088084409788	6.167132244036877E-4
62-63	2.4668528976147507E-5	3.7002793464221257E-4	0.0	0.22882527478274428	6.167132244036877E-4
64-65	2.4668528976147507E-5	4.193649925945076E-4	0.0	0.24621658771092825	6.290474888917615E-4
66-67	2.4668528976147507E-5	4.193649925945076E-4	0.0	0.26540870325437105	6.413817533798352E-4
68-69	2.4668528976147507E-5	4.193649925945076E-4	0.0	0.28522986628670555	6.413817533798352E-4
70-71	2.4668528976147507E-5	4.563677860587289E-4	0.0	0.30648180399965663	6.413817533798352E-4
72-73	2.4668528976147507E-5	4.933705795229502E-4	0.0	0.3253038916084572	7.770586627486464E-4
74-75	2.4668528976147507E-5	4.933705795229502E-4	0.0	0.34593911609700456	8.140614562128677E-4
76-77	2.4668528976147507E-5	5.180391084990976E-4	1.2334264488073753E-5	0.36580961618729135	8.263957207009414E-4
78-79	2.4668528976147507E-5	5.180391084990976E-4	2.4668528976147507E-5	0.388652674019204	8.387299851890152E-4
80-81	2.4668528976147507E-5	5.180391084990976E-4	2.4668528976147507E-5	0.41080501303978445	8.387299851890152E-4
82-83	2.4668528976147507E-5	5.180391084990976E-4	2.4668528976147507E-5	0.4315882487021887	8.880670431413102E-4
84-85	2.4668528976147507E-5	5.180391084990976E-4	2.4668528976147507E-5	0.4534568996395435	8.880670431413102E-4
86-87	2.4668528976147507E-5	5.180391084990976E-4	2.4668528976147507E-5	0.4757202470405166	9.127355721174577E-4
88-89	2.4668528976147507E-5	5.180391084990976E-4	2.4668528976147507E-5	0.5004257788101283	9.374041010936052E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	4035	0.0	29.162922	1
TACCTGG	4405	0.0	25.340185	2
GTACATG	12215	0.0	24.25909	1
TACATGG	12535	0.0	22.736036	2
ACATGGG	12765	0.0	21.173372	3
TATCACG	375	0.0	20.266397	2
AGTACAT	8935	0.0	19.031645	2
GAGTACA	9690	0.0	18.928429	1
ACCTGGG	5815	0.0	18.21603	3
AGAGTAC	11150	0.0	17.94861	10-11
CATGGGG	10195	0.0	17.798143	4
GTATCAA	16355	0.0	17.710491	1
GTATAGG	1025	0.0	17.661896	1
GTATATA	905	0.0	17.371725	1
GTATAGC	1065	0.0	16.998539	1
TACACTA	765	0.0	16.1362	5
GTATAAG	1045	0.0	15.956196	1
TCAACGC	18090	0.0	15.780999	4
TAGACTG	1445	0.0	15.771129	5
CAACGCA	18220	0.0	15.634797	5
>>END_MODULE