FastQCFastQC Report
Mon 15 Aug 2016
SRR2921475_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921475_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7464921
Sequences flagged as poor quality0
Sequence length98
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75170.10069764971390857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA136750.036.3875921
GGTATCA88050.029.8511921
TCAACGC172650.028.5149174
TATCAAC177850.027.8617972
CAACGCA177100.027.8243055
ATCAACG179300.027.457523
AACGCAG185800.026.5892166
GTACATG250700.025.4302371
TACATGG258800.024.230322
ACATGGG260900.023.0865273
ACGCAGA223100.021.9546367
GAGTACA187050.021.2131181
CGCAGAG233250.020.9560498
GTACCTG70300.020.8876861
CATGGGG207800.020.4488354
AGTACAT178800.020.4328122
TACCTGG71000.019.8851872
GCAGAGT264350.018.2132369
GTATAGG15000.018.1057171
AGAGTAC204900.018.03138410-11
Produced by FastQC (version 0.11.5)