##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921475_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7464921
Sequences flagged as poor quality	0
Sequence length	98
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.198195131602866	31.0	31.0	34.0	27.0	34.0
2	30.30151893101079	31.0	31.0	34.0	26.0	34.0
3	30.436007963111734	31.0	31.0	34.0	26.0	34.0
4	33.919150651426854	37.0	35.0	37.0	30.0	37.0
5	33.89735243011949	37.0	35.0	37.0	30.0	37.0
6	33.990585834732876	37.0	35.0	37.0	30.0	37.0
7	33.86413265458536	37.0	35.0	37.0	30.0	37.0
8	33.83918610793068	37.0	35.0	37.0	30.0	37.0
9	35.2700348737783	39.0	35.0	39.0	30.0	39.0
10-11	35.14179667808942	39.0	35.0	39.0	29.0	39.0
12-13	34.977011009225684	39.0	35.0	39.0	28.0	39.0
14-15	35.99379444203093	40.0	36.0	41.0	27.0	41.0
16-17	35.841967718077655	39.5	36.0	41.0	27.0	41.0
18-19	35.646922385380904	39.0	36.0	41.0	27.0	41.0
20-21	35.518195169111635	39.0	36.0	41.0	27.0	41.0
22-23	35.556752241584334	39.0	36.0	41.0	27.0	41.0
24-25	35.422548678010116	39.0	36.0	41.0	26.0	41.0
26-27	35.15001478247392	39.0	35.0	40.5	25.0	41.0
28-29	34.90880184800348	39.0	35.0	40.0	24.0	41.0
30-31	34.64410547680277	38.5	34.5	40.0	23.5	41.0
32-33	34.367503889190516	38.0	34.0	40.0	21.5	41.0
34-35	33.950695124569975	38.0	33.5	40.0	17.5	41.0
36-37	33.56869081400862	38.0	33.0	40.0	15.5	41.0
38-39	33.327396431924726	38.0	33.0	40.0	14.5	41.0
40-41	33.04266850245301	38.0	32.5	40.0	9.0	41.0
42-43	32.73736607527394	38.0	32.0	40.0	9.0	41.0
44-45	32.413145765373805	37.5	31.0	40.0	8.0	41.0
46-47	32.1186570494182	37.0	31.0	40.0	7.0	41.0
48-49	31.902835006559346	37.0	31.0	40.0	4.0	41.0
50-51	31.152129272366043	36.0	29.5	39.0	2.0	40.0
52-53	31.65432581269112	36.5	31.0	39.0	2.0	40.0
54-55	32.06665897736895	37.0	31.0	40.0	2.0	40.5
56-57	31.897668977340818	37.0	31.0	40.0	2.0	41.0
58-59	31.55036985655977	36.0	31.0	39.0	2.0	41.0
60-61	31.080554435874138	35.5	30.5	39.0	2.0	40.0
62-63	30.575464361913543	35.0	29.5	39.0	2.0	40.0
64-65	30.019852185977587	35.0	29.0	38.0	2.0	40.0
66-67	29.520552260365516	34.5	28.5	37.5	2.0	40.0
68-69	28.994796462012125	34.0	28.0	37.0	2.0	39.0
70-71	28.46983296943129	34.0	26.5	36.0	2.0	39.0
72-73	27.91544378031596	34.0	26.0	36.0	2.0	38.5
74-75	27.321297238108748	33.0	25.5	35.0	2.0	37.5
76-77	26.737485969375964	33.0	24.5	35.0	2.0	37.0
78-79	26.181375194727444	32.0	23.5	35.0	2.0	36.0
80-81	25.609180860721768	32.0	20.5	35.0	2.0	36.0
82-83	24.985406342545353	31.5	18.5	34.0	2.0	35.0
84-85	24.37020378380428	31.0	14.5	34.0	2.0	35.0
86-87	23.745987197989102	31.0	7.0	34.0	2.0	35.0
88-89	23.093919078313085	30.0	2.0	34.0	2.0	35.0
90-91	22.35990729171816	30.0	2.0	34.0	2.0	35.0
92-93	21.53207656182832	29.0	2.0	34.0	2.0	35.0
94-95	20.619025506097117	29.0	2.0	33.5	2.0	35.0
96-97	19.721056592561396	27.0	2.0	33.0	2.0	34.0
98	19.10062517741313	26.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	271174.0
3	45581.0
4	34326.0
5	29000.0
6	26867.0
7	28499.0
8	32045.0
9	36760.0
10	41027.0
11	44238.0
12	48862.0
13	54899.0
14	46818.0
15	46487.0
16	51993.0
17	58317.0
18	65777.0
19	64481.0
20	67721.0
21	73241.0
22	79980.0
23	88417.0
24	98248.0
25	110182.0
26	124346.0
27	141888.0
28	163487.0
29	189791.0
30	225051.0
31	270762.0
32	336938.0
33	434214.0
34	581798.0
35	809654.0
36	1086738.0
37	1094604.0
38	442078.0
39	18632.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.52494976557267	16.575543856324153	12.941597228653329	24.957909149449844
2	16.40819191964409	18.080749948466774	38.40262552729411	27.108432604595023
3	20.854950511373097	21.193493413713984	27.14082778880027	30.81072828611265
4	14.275481668296534	13.914525322970817	33.52277844974008	38.287214558992574
5	16.136874610249883	35.307204860971474	32.21303801601843	16.342882512760212
6	36.75268910606744	31.94321006917774	15.796643232080154	15.507457592674662
7	32.49397381420236	29.640390897289887	18.220256186272064	19.645379102235687
8	28.795471638046184	33.48294421239975	18.02060760560825	19.700976543945817
9	27.183637574543013	16.346308048294347	18.277221841058232	38.192832536104405
10-11	27.525770937549048	24.532082279159816	25.10336745341891	22.83877932987222
12-13	27.324917322213032	21.359007184622484	26.05899880447207	25.257076688692415
14-15	24.330201375192605	23.008627896322153	23.397500547479154	29.263670181006084
16-17	25.072980258737015	26.229396730245924	23.999088404102263	24.698534606914798
18-19	25.113285273949813	25.422914376471756	25.416433751763655	24.047366597814776
20-21	25.520334532901234	24.617119028589993	25.435056208880074	24.4274902296287
22-23	25.319295415928515	24.218894049692924	25.384520278777284	25.07729025560127
24-25	25.08241593802217	24.510130978720557	25.42248913660646	24.984963946650815
26-27	25.037861700664937	24.721211783581573	25.449706367771242	24.79122014798225
28-29	25.017894638540366	24.82550339124937	25.402067069581236	24.75453490062903
30-31	25.175861261263073	24.733880012693525	25.23552886385928	24.854729862184126
32-33	25.007688566876595	24.7825123864655	25.14605596955073	25.06374307710718
34-35	25.114894930580846	24.757793745634928	25.34303375611278	24.784277567671445
36-37	25.171580722563142	24.739500427254015	25.229792691647706	24.859126158535137
38-39	25.14556389376763	24.733957587627017	25.18924687283362	24.93123164577173
40-41	25.289939032215493	24.741053143851595	25.224222549760444	24.744785274172465
42-43	25.191541096889107	24.668356981783347	25.176825428190604	24.963276493136945
44-45	25.08532226709999	24.754416714740373	25.17342054229832	24.98684047586132
46-47	25.249327294356487	24.736351367401642	25.18366594478683	24.830655393455032
48-49	25.268126079714758	24.855912359813807	24.934769407262685	24.941192153208746
50-51	24.998490028023866	25.049643905850736	25.023585526597085	24.928280539528306
52-53	25.18494253023836	25.010698207836224	24.965533238438827	24.838826023486593
54-55	25.092183229938154	24.777500777274415	25.114751977881276	25.015564014906154
56-57	25.090725167674304	24.921022252489198	25.092469267314517	24.89578331252198
58-59	25.174814346436982	24.861190703426107	25.076944972865604	24.887049977271307
60-61	25.234102225120857	24.725356191577127	25.1271966084316	24.913344974870412
62-63	25.073646035855024	24.97452650203401	25.046494058114256	24.905333403996714
64-65	25.09995387573174	24.78831197032528	25.09577116974593	25.01596298419705
66-67	25.122509950227972	24.874229226195784	25.039918139853757	24.96334268372248
68-69	25.053301982117905	24.86712467885981	25.11369327432388	24.965880064698414
70-71	24.96065310197052	24.797766636145784	25.137842023887053	25.103738237996637
72-73	25.182476962630457	24.748489158380213	25.02126668772979	25.047767191259545
74-75	24.95371432531371	25.00079727486741	25.035083458194872	25.010404941624003
76-77	25.157313918795314	24.803230169711895	24.94336308637214	25.096092825120646
78-79	25.076254135009197	24.748757167444598	25.138292309749204	25.036696387796997
80-81	25.050379547229333	24.876932606531128	25.068284051426637	25.0044037948129
82-83	25.132279779059807	24.86083897376882	24.950742720431247	25.056138526740124
84-85	25.14310390736939	24.91101206145647	24.859889827852825	25.08599420332131
86-87	25.12332663459047	24.88083443044842	25.038847259176762	24.956991675784344
88-89	25.00292397700173	24.667363127256998	25.048603967537325	25.28110892820395
90-91	25.062769101749865	24.720233760235892	25.032356802477768	25.184640335536475
92-93	25.136612426274368	24.870552913985776	25.01831366778226	24.974520991957593
94-95	25.234183165512047	24.701179777685127	24.964142111627915	25.100494945174912
96-97	25.1626311760957	24.661671173770376	25.001063080791987	25.174634569341936
98	25.169772633110398	24.782195709459	24.83591258197979	25.21211907545081
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	850.0
1	627.0
2	607.5
3	878.0
4	793.0
5	729.0
6	1203.5
7	2484.0
8	3724.5
9	3710.5
10	2965.5
11	2567.5
12	2764.5
13	3297.5
14	3941.0
15	4465.5
16	4968.0
17	5372.0
18	5505.5
19	5357.5
20	5098.5
21	4754.5
22	4471.0
23	4527.5
24	4794.5
25	5456.0
26	6658.5
27	8567.0
28	11579.0
29	15858.0
30	21323.0
31	28076.0
32	36225.0
33	44698.5
34	55207.0
35	68599.5
36	83628.5
37	100337.5
38	118613.0
39	138249.5
40	158792.0
41	181947.0
42	206262.0
43	229257.0
44	252481.5
45	276862.5
46	302088.5
47	327526.0
48	342853.5
49	347249.0
50	348961.0
51	350928.0
52	357096.5
53	364348.5
54	364308.0
55	352260.0
56	331419.5
57	310075.0
58	286272.5
59	255813.5
60	224265.5
61	190069.0
62	153682.5
63	117873.0
64	86950.0
65	65973.5
66	50812.5
67	38199.5
68	28628.5
69	22037.5
70	16901.0
71	12822.5
72	9921.0
73	7620.5
74	5834.0
75	4359.5
76	2959.0
77	1876.0
78	1182.0
79	725.5
80	439.5
81	270.0
82	155.0
83	93.0
84	58.0
85	44.0
86	40.5
87	31.5
88	27.5
89	29.5
90	23.5
91	12.5
92	8.5
93	8.5
94	9.5
95	10.0
96	7.0
97	5.0
98	4.5
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4789467966238357
2	0.049766099333134264
3	0.05919687562668111
4	0.05606221418820106
5	0.06715409312436126
6	0.06707371719004127
7	0.04446128766801417
8	0.02089774292320039
9	0.012619021688240237
10-11	0.015211145570060288
12-13	0.011446872646073549
14-15	0.015626421230713627
16-17	0.014568138095500276
18-19	0.0565377717995944
20-21	0.0857611219194416
22-23	0.10237214834557526
24-25	0.07167523942986134
26-27	0.08742892255658165
28-29	0.08950530085984836
30-31	0.059257157577421116
32-33	0.034052604173573975
34-35	0.40447179548182766
36-37	0.6323241732899786
38-39	0.5795908623815309
40-41	0.5745070845357908
42-43	0.5473802067027903
44-45	0.5316935035213367
46-47	0.5216264177477564
48-49	0.5116062179358629
50-51	0.5262815239437899
52-53	0.6089012864302248
54-55	0.5780838136130309
56-57	0.53431911737579
58-59	0.5115258420015429
60-61	0.5252835227593166
62-63	0.5364758716133767
64-65	0.5558933577461839
66-67	0.5824977920061043
68-69	0.5359668240293501
70-71	0.4839568965297824
72-73	0.46716502425142875
74-75	0.4468366108629951
76-77	0.46183342060820204
78-79	0.45563107767650857
80-81	0.45318630967427515
82-83	0.47299227948962885
84-85	0.4385043056718216
86-87	0.44112322153174827
88-89	0.4684845291731822
90-91	0.4516725629112485
92-93	0.4462605833337017
94-95	0.4581294296349553
96-97	0.45129747521775515
98	0.44694377877542174
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	7464921.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.88970704151026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.085616907618	31.46122810806491
2	14.110341736575686	12.668182136209555
3	5.978993968126986	8.05185862896532
4	2.9034499178121878	5.213400648811446
5	1.741229268289797	3.9081635872816136
6	1.0693450471020667	2.880155153442106
7	0.7448274747465709	2.3404560996489012
8	0.543107640517925	1.9503954299884416
9	0.4059555131475015	1.6400901651369582
>10	2.1712322848663597	17.696323211352365
>50	0.16489006580226803	5.066180942026885
>100	0.07846182450729022	6.077463563024729
>500	0.0021286677408208906	0.6263862228114505
>1k	3.897057799799737E-4	0.3185616980318732
>5k	2.997736662293983E-5	0.1011544052034273
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7517	0.10069764971390857	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.679197810666717E-5
2	0.0	0.0	0.0	0.0	2.679197810666717E-5
3	0.0	0.0	0.0	0.0	2.679197810666717E-5
4	0.0	0.0	0.0	0.0	2.679197810666717E-5
5	0.0	0.0	0.0	0.0	2.679197810666717E-5
6	0.0	0.0	0.0	0.0	5.358395621333434E-5
7	0.0	0.0	0.0	0.0	5.358395621333434E-5
8	0.0	0.0	0.0	0.0	5.358395621333434E-5
9	0.0	0.0	0.0	0.0	5.358395621333434E-5
10-11	0.0	0.0	0.0	0.0	5.358395621333434E-5
12-13	0.0	0.0	0.0	0.0	1.0046991790000189E-4
14-15	0.0	2.0093983580000377E-5	0.0	0.0	1.2056390148000226E-4
16-17	0.0	2.679197810666717E-5	0.0	6.697994526666793E-6	1.3395989053333586E-4
18-19	0.0	2.679197810666717E-5	0.0	2.679197810666717E-5	1.4735587958666942E-4
20-21	0.0	2.679197810666717E-5	0.0	2.679197810666717E-5	1.540538741133362E-4
22-23	0.0	2.679197810666717E-5	0.0	4.0187967160000755E-5	1.6075186864000302E-4
24-25	0.0	2.679197810666717E-5	0.0	7.367793979333471E-5	1.741478576933366E-4
26-27	1.3395989053333585E-5	2.679197810666717E-5	0.0	1.6744986316666983E-4	1.9424184127333696E-4
28-29	1.3395989053333585E-5	2.679197810666717E-5	0.0	5.961215128733445E-4	2.1433582485333737E-4
30-31	1.3395989053333585E-5	3.3489972633333964E-5	0.0	0.0023041101171733767	2.2103381938000415E-4
32-33	1.3395989053333585E-5	4.688596168666755E-5	0.0	0.0054454695501801025	2.2773181390667096E-4
34-35	2.679197810666717E-5	5.358395621333434E-5	0.0	0.009276722419433508	2.4112780296000453E-4
36-37	2.679197810666717E-5	6.028195074000113E-5	0.0	0.015425481394913624	2.4782579748667134E-4
38-39	2.679197810666717E-5	8.70739288466683E-5	0.0	0.024675411836240464	2.545237920133381E-4
40-41	2.679197810666717E-5	1.0716791242666868E-4	0.0	0.034863061511300655	2.545237920133381E-4
42-43	2.679197810666717E-5	1.2726189600666907E-4	0.0	0.04450147563517417	2.612217865400049E-4
44-45	2.679197810666717E-5	1.3395989053333586E-4	0.0	0.05575410643997438	2.813157701200053E-4
46-47	2.679197810666717E-5	1.741478576933366E-4	0.0	0.06716748911341461	3.081077482266724E-4
48-49	4.0187967160000755E-5	1.875438467466702E-4	0.0	0.07862775774854147	3.5499370991334E-4
50-51	4.0187967160000755E-5	2.0093983580000377E-4	0.0	0.0904028321264217	3.616917044400068E-4
52-53	4.0187967160000755E-5	2.0093983580000377E-4	0.0	0.10214441653166859	3.88483682546674E-4
54-55	4.0187967160000755E-5	2.0093983580000377E-4	0.0	0.11390609492049547	3.88483682546674E-4
56-57	4.0187967160000755E-5	2.0763783032667058E-4	0.0	0.12689350630770238	3.88483682546674E-4
58-59	4.0187967160000755E-5	2.1433582485333737E-4	0.0	0.13986752170585595	4.0187967160000755E-4
60-61	4.0187967160000755E-5	2.2773181390667096E-4	0.0	0.1526071072955762	4.1527566065334117E-4
62-63	4.688596168666755E-5	2.2773181390667096E-4	0.0	0.1657150825842631	4.1527566065334117E-4
64-65	5.358395621333434E-5	2.2773181390667096E-4	0.0	0.17869579597694335	4.1527566065334117E-4
66-67	5.358395621333434E-5	2.4112780296000453E-4	0.0	0.19312327618738362	4.21973655180008E-4
68-69	5.358395621333434E-5	2.4112780296000453E-4	0.0	0.20807989796543058	4.2867164970667473E-4
70-71	6.028195074000113E-5	2.4782579748667134E-4	0.0	0.22371301719067088	4.420676387600083E-4
72-73	6.697994526666793E-5	2.545237920133381E-4	0.0	0.23925236449253784	4.755576113933423E-4
74-75	6.697994526666793E-5	2.679197810666717E-4	0.0	0.25453718800239145	4.956515949733427E-4
76-77	6.697994526666793E-5	2.679197810666717E-4	0.0	0.27140273822053845	5.224435730800098E-4
78-79	6.697994526666793E-5	2.679197810666717E-4	6.697994526666793E-6	0.2884089463237454	5.492355511866769E-4
80-81	6.697994526666793E-5	2.7461777559333847E-4	2.0093983580000377E-5	0.3061251418467791	5.760275292933442E-4
82-83	6.697994526666793E-5	2.9471175917333885E-4	3.3489972633333964E-5	0.32402888121655943	6.296114855066785E-4
84-85	6.697994526666793E-5	3.148057427533392E-4	4.0187967160000755E-5	0.3418455466574931	6.296114855066785E-4
86	6.697994526666793E-5	3.2150373728000604E-4	4.0187967160000755E-5	0.35519464974914	6.296114855066785E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	13675	0.0	36.387592	1
GGTATCA	8805	0.0	29.851192	1
TCAACGC	17265	0.0	28.514917	4
TATCAAC	17785	0.0	27.861797	2
CAACGCA	17710	0.0	27.824305	5
ATCAACG	17930	0.0	27.45752	3
AACGCAG	18580	0.0	26.589216	6
GTACATG	25070	0.0	25.430237	1
TACATGG	25880	0.0	24.23032	2
ACATGGG	26090	0.0	23.086527	3
ACGCAGA	22310	0.0	21.954636	7
GAGTACA	18705	0.0	21.213118	1
CGCAGAG	23325	0.0	20.956049	8
GTACCTG	7030	0.0	20.887686	1
CATGGGG	20780	0.0	20.448835	4
AGTACAT	17880	0.0	20.432812	2
TACCTGG	7100	0.0	19.885187	2
GCAGAGT	26435	0.0	18.213236	9
GTATAGG	1500	0.0	18.105717	1
AGAGTAC	20490	0.0	18.031384	10-11
>>END_MODULE