FastQCFastQC Report
Mon 15 Aug 2016
SRR2921475_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921475_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7464921
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76460.10242573230178859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA141400.036.750371
GGTATCA90150.031.7009241
TCAACGC173400.029.3080434
CAACGCA175250.028.9285355
TATCAAC183650.027.9548852
ATCAACG184900.027.5105233
AACGCAG190050.026.7765586
GTACATG246450.025.7238311
TACATGG258700.024.012332
GTACCTG68750.022.5855831
ACGCAGA224550.022.5582477
ACATGGG260850.022.5047363
GAGTACA187650.022.2098641
AGTACAT180500.021.2070752
CGCAGAG240200.021.0291968
CATGGGG205600.019.404764
TACCTGG79550.018.7461642
GCAGAGT272500.018.2574489
AGAGTAC210700.018.22722410-11
GTGTACG13100.017.816041
Produced by FastQC (version 0.11.5)