##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921475_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7464921
Sequences flagged as poor quality	0
Sequence length	101
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.885161946121062	33.0	31.0	34.0	30.0	34.0
2	32.004510965353816	34.0	31.0	34.0	30.0	34.0
3	32.12803283517669	34.0	31.0	34.0	30.0	34.0
4	35.66553751874936	37.0	35.0	37.0	35.0	37.0
5	35.45429764092614	37.0	35.0	37.0	33.0	37.0
6	35.48980183447353	37.0	35.0	37.0	33.0	37.0
7	35.414799969082054	37.0	35.0	37.0	33.0	37.0
8	35.365800656162335	37.0	35.0	37.0	33.0	37.0
9	36.979555979226035	39.0	37.0	39.0	33.0	39.0
10-11	36.88953185171015	39.0	37.0	39.0	33.0	39.0
12-13	36.76524674541097	39.0	37.0	39.0	33.0	39.0
14-15	38.05360867181314	40.0	38.0	41.0	33.0	41.0
16-17	37.947658186871635	40.0	38.0	41.0	33.0	41.0
18-19	37.83393548304128	40.0	38.0	41.0	33.0	41.0
20-21	37.71380119093022	40.0	38.0	41.0	32.0	41.0
22-23	37.67795158180508	40.0	38.0	41.0	32.5	41.0
24-25	37.72796691351456	40.0	38.0	41.0	32.5	41.0
26-27	37.61329771607764	40.0	38.0	41.0	32.0	41.0
28-29	37.4754556009367	40.0	38.0	41.0	32.0	41.0
30-31	37.329847295101985	40.0	37.0	41.0	32.0	41.0
32-33	37.1654831310338	40.0	37.0	41.0	31.0	41.0
34-35	37.01219128775777	40.0	37.0	41.0	31.0	41.0
36-37	36.91158452982958	40.0	37.0	41.0	31.0	41.0
38-39	36.87556251432534	40.0	37.0	41.0	31.0	41.0
40-41	36.70383671843279	40.0	36.0	41.0	30.0	41.0
42-43	36.451175973597046	39.0	36.0	41.0	30.0	41.0
44-45	36.23554783767973	39.0	35.5	41.0	30.0	41.0
46-47	35.94933295342308	39.0	35.0	40.0	29.0	41.0
48-49	35.602651320757445	39.0	35.0	40.0	28.0	41.0
50-51	35.303385126245814	38.0	35.0	40.0	27.0	41.0
52-53	34.97152930352512	38.0	34.0	40.0	26.5	41.0
54-55	34.619953513238784	38.0	34.0	40.0	26.0	41.0
56-57	34.21233031133217	37.5	33.5	40.0	25.0	41.0
58-59	33.7904139909853	37.0	33.0	39.5	24.0	41.0
60-61	33.33856332839959	36.0	32.5	39.0	23.0	40.5
62-63	32.872098914375655	36.0	32.0	39.0	22.0	40.0
64-65	32.330197868135514	35.0	31.0	38.5	20.5	40.0
66-67	31.780195731475256	35.0	31.0	38.0	18.5	40.0
68-69	31.252485860198654	35.0	30.5	37.0	17.5	39.0
70-71	30.68801746193965	34.0	30.0	36.5	12.5	39.0
72-73	30.127895191389165	34.0	29.0	36.0	8.0	38.5
74-75	29.601761693124416	34.0	29.0	35.0	7.0	37.5
76-77	28.379588544875425	32.0	27.0	34.5	6.5	36.0
78-79	29.30711597617711	33.5	29.0	35.0	6.5	36.0
80-81	29.33629625819215	34.0	29.0	35.0	2.0	36.0
82-83	28.995307251074728	34.0	29.0	35.0	2.0	36.0
84-85	28.49776220002864	33.5	29.0	35.0	2.0	35.0
86-87	27.9641584284683	33.0	27.0	35.0	2.0	35.0
88-89	27.408524618010023	33.0	26.5	35.0	2.0	35.0
90-91	26.90983997017517	32.5	25.0	34.5	2.0	35.0
92-93	26.444412271744067	32.0	25.0	34.0	2.0	35.0
94-95	25.91350598351945	32.0	24.0	34.0	2.0	35.0
96-97	25.105024956057807	32.0	20.5	34.0	2.0	35.0
98-99	24.05452656498307	31.0	8.5	34.0	2.0	35.0
100-101	20.494351112356043	27.0	2.0	32.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	54210.0
3	27960.0
4	16908.0
5	12508.0
6	11035.0
7	11571.0
8	13515.0
9	15846.0
10	18852.0
11	21890.0
12	25805.0
13	29616.0
14	33485.0
15	37687.0
16	42571.0
17	48484.0
18	50366.0
19	46747.0
20	47381.0
21	49860.0
22	53557.0
23	58634.0
24	66533.0
25	77123.0
26	91363.0
27	110014.0
28	133431.0
29	161026.0
30	196140.0
31	244530.0
32	311303.0
33	415058.0
34	585137.0
35	871156.0
36	1325014.0
37	1537982.0
38	594396.0
39	16227.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.67723590171407	17.068047827096574	13.299841751739441	24.954874519449916
2	16.50419341343331	18.331620656132866	38.018942196441195	27.14524373399263
3	20.85497192771257	21.31959913076318	27.3567920815371	30.468636859987146
4	14.483989299793832	14.229003553018552	33.43762151633079	37.84938563085683
5	16.1750208609822	35.301537476826525	32.23742121956738	16.286020442623894
6	36.806572153599575	32.04210394810973	15.677683301647422	15.473640596643282
7	32.55825831594721	29.61737480013722	18.230488318710133	19.593878565205436
8	28.834233166776315	33.54534465931799	17.95856305212633	19.661859121779365
9	27.09083356596625	16.341235343791222	18.25763444908638	38.31029664115616
10-11	27.522725115140762	24.564129127139967	25.118982363229613	22.794163394489654
12-13	27.318009653589726	21.32829881544239	26.121397628471215	25.232293902496668
14-15	24.270933091838582	23.00689250635053	23.46843487580466	29.25373952600623
16-17	25.028819838903715	26.245467638528698	24.116126278634322	24.60958624393326
18-19	25.05692620917312	25.45138096688286	25.47812286734014	24.013569956603877
20-21	25.450448790573553	24.59350233523855	25.575501803269134	24.38054707091876
22-23	25.24103290194682	24.167363270956677	25.56141168492691	25.030192142169593
24-25	25.006184339136027	24.47167787083991	25.56767945102901	24.954458338995053
26-27	24.91462974108754	24.70114044965198	25.61557966023937	24.768650149021113
28-29	24.945005154960683	24.81644099156133	25.54579306100554	24.692760792472452
30-31	25.084841036047617	24.706171872650398	25.387197642556213	24.821789448745765
32-33	24.907406954648177	24.703924305365444	25.34050923713298	25.0481595028534
34-35	25.03315105278205	24.725286355699208	25.520523107402443	24.721039484116297
36-37	25.009284401722798	24.74096787141492	25.416364555182035	24.833383171680243
38-39	25.044463313453885	24.73285669545586	25.38341548051496	24.839264510575298
40-41	25.163043518311273	24.70552547514568	25.376089237355814	24.75534176918723
42-43	25.122880589893814	24.70933225340104	25.293792467994713	24.873994688710432
44-45	24.95641503962321	24.693729613877945	25.3508015713831	24.99905377511574
46-47	25.062110463399694	24.746578656940073	25.378466322605664	24.812844557054564
48-49	25.133452431053012	24.837259871862894	25.138342086598207	24.890945610485886
50-51	24.920576654881888	25.052488937534413	25.15229562069709	24.874638786886607
52-53	25.150174041199342	24.943871235970153	25.061644684812517	24.844310038017987
54-55	25.053073578795594	24.75731237835142	25.13048524663691	25.05912879621608
56-57	25.034181052002086	24.950473310111025	25.16133349373354	24.854012144153344
58-59	25.139619414813154	24.844281794675886	25.119545324877073	24.89655346563389
60-61	25.16782558236661	24.78049326812084	25.19545629220256	24.856224857309993
62-63	25.004342597116246	24.961429095956692	25.126745889839853	24.90748241708721
64-65	25.082114457925346	24.814063126199144	25.127094458738537	24.976727957136973
66-67	25.078800275232254	24.88429638378081	25.066035706084705	24.970867634902234
68-69	25.012695386131085	24.897714763475758	25.16103218499039	24.92855766540277
70-71	24.982362027917972	24.78117660614067	25.202116556649845	25.034344809291518
72-73	25.193287580643975	24.799710824064316	24.999768846854007	25.00723274843771
74-75	24.926242804966776	25.02471975951653	25.02438468991956	25.024652745597137
76-77	25.059262593553065	24.832585356677374	25.055654837886138	25.052497211883423
78-79	25.004948487800444	24.790734098194488	25.225389961953116	24.978927452051952
80-81	24.93060742182828	24.96384054879685	25.149403341416708	24.95614868795816
82-83	25.062492889779758	24.92150785845995	25.034534398324286	24.981464853436
84-85	25.02443394607844	25.008402570942184	24.978282611638967	24.98888087134041
86-87	24.98237506177027	24.98836348366284	25.126526368604203	24.90273508596269
88-89	24.880497115837702	24.796052226682647	25.13445799928975	25.188992658189903
90-91	24.96725462362032	24.850316910859206	25.108799153777657	25.073629311742824
92-93	25.047287278947973	25.022305067381605	25.101413170262752	24.82899448340767
94-95	25.06773057282196	24.88494761912258	25.00010885121111	25.047212956844344
96-97	24.98784248685783	24.825159554800578	25.129572966513585	25.057424991828007
98-99	24.977957993112952	24.897526941086582	25.066420762585786	25.058094303214684
100-101	25.552806938572093	24.285035823566922	25.132509686028005	25.02964755183298
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	242.0
1	206.5
2	339.5
3	564.5
4	463.0
5	368.5
6	828.0
7	2051.0
8	3174.0
9	3065.0
10	2187.5
11	1539.5
12	1385.0
13	1433.5
14	1559.0
15	1693.5
16	1852.0
17	2006.5
18	2147.5
19	2312.0
20	2495.0
21	2761.0
22	3000.5
23	3311.5
24	3695.5
25	4434.5
26	5484.0
27	7044.5
28	9497.0
29	12922.5
30	17708.5
31	23403.0
32	30355.5
33	38467.0
34	47001.5
35	57598.0
36	71659.5
37	87714.0
38	104343.0
39	122229.0
40	142538.5
41	163500.5
42	185596.5
43	209381.5
44	233795.5
45	258726.0
46	279641.5
47	301737.0
48	328545.0
49	343379.5
50	344411.0
51	346732.0
52	353547.5
53	359387.5
54	360354.5
55	351894.0
56	330905.5
57	305036.0
58	282491.5
59	255609.5
60	222811.0
61	190422.0
62	155517.5
63	118253.0
64	86681.0
65	65116.0
66	49874.0
67	38266.0
68	28785.0
69	21756.5
70	17026.5
71	13141.0
72	9831.0
73	7630.5
74	5926.5
75	4432.5
76	3187.0
77	1986.5
78	1171.5
79	658.0
80	369.5
81	199.0
82	106.5
83	61.5
84	29.5
85	16.5
86	11.5
87	7.5
88	5.5
89	1.5
90	1.0
91	2.5
92	2.0
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.31412254731161926
2	0.0
3	0.0059210271615734444
4	0.060335534696214464
5	3.750876934933404E-4
6	0.06320227635362785
7	0.0727134285814947
8	0.10201045664113524
9	0.09134724935468172
10-11	0.09501775035529511
12-13	0.09475652856875512
14-15	0.06319557835910118
16-17	0.035010417390887325
18-19	0.03815847481842072
20-21	0.02794403316525386
22-23	0.04190935175335412
24-25	0.03379808038156063
26-27	0.020750387043613722
28-29	0.02306119515531377
30-31	0.0030207955315267234
32-33	0.005391885593966768
34-35	0.008077781399160152
36-37	0.0105694353630802
38-39	0.0158541530446203
40-41	0.02008728558547371
42-43	0.003422675203126731
44-45	0.014072486500526933
46-47	0.024199854224847123
48-49	0.0024447680022333792
50-51	0.00657743062518679
52-53	0.0022170361883267083
54-55	0.003804460891146738
56-57	0.003469561164813399
58-59	9.98001184473352E-4
60-61	0.0053650936158601
62-63	0.01432701029254027
64-65	0.006597524608766791
66-67	0.03830583069800739
68-69	0.060730716373287814
70-71	0.012203746027586896
72-73	0.031293030428587255
74-75	0.05069712057234095
76-77	0.303030668375459
78-79	0.07463575301064808
80-81	0.03332252277016729
82-83	0.02775648931850719
84-85	0.01917635832984703
86-87	0.11877553694138224
88-89	0.5273732970516366
90-91	0.12953921414573577
92-93	0.04838631246064091
94-95	0.008084479393686818
96-97	0.005371791610386768
98-99	0.010234535636746858
100-101	0.007515149858920142
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	7464921.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.677928251277855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.41625083662913	25.55903378912088
2	16.52369837268283	13.11252236954132
3	6.891181700243178	8.202834392063032
4	3.572276501089275	5.669621228157845
5	2.105199663732922	4.1764980606104585
6	1.3558373251726918	3.2278089665163927
7	0.9497815423814766	2.6379754725100173
8	0.6707512785837716	2.129121688487982
9	0.5149766377837831	1.8389885476562327
>10	2.690895197610601	19.460619873049367
>50	0.2017949885803852	5.5285657211387385
>100	0.1036324036063875	7.143639316219384
>500	0.0031819944753023934	0.8324493852943273
>1k	5.077100897603017E-4	0.3776360008739235
>5k	3.384733847048501E-5	0.10268518875994637
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7646	0.10242573230178859	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.3395989053333585E-5	0.0
3	0.0	1.3395989053333585E-5	0.0	1.3395989053333585E-5	0.0
4	0.0	1.3395989053333585E-5	0.0	1.3395989053333585E-5	0.0
5	0.0	1.3395989053333585E-5	0.0	1.3395989053333585E-5	0.0
6	0.0	1.3395989053333585E-5	0.0	1.3395989053333585E-5	1.3395989053333585E-5
7	0.0	1.3395989053333585E-5	0.0	1.3395989053333585E-5	1.3395989053333585E-5
8	0.0	1.3395989053333585E-5	0.0	1.3395989053333585E-5	1.3395989053333585E-5
9	0.0	1.3395989053333585E-5	0.0	1.3395989053333585E-5	1.3395989053333585E-5
10-11	0.0	1.3395989053333585E-5	0.0	1.3395989053333585E-5	1.3395989053333585E-5
12-13	0.0	2.0093983580000377E-5	0.0	1.3395989053333585E-5	6.697994526666793E-5
14-15	0.0	7.367793979333471E-5	0.0	1.3395989053333585E-5	8.707392884666829E-5
16-17	0.0	1.2056390148000226E-4	0.0	2.0093983580000377E-5	1.0046991790000189E-4
18-19	0.0	1.2056390148000226E-4	0.0	4.0187967160000755E-5	1.0716791242666868E-4
20-21	0.0	1.2056390148000226E-4	0.0	5.358395621333434E-5	1.2726189600666907E-4
22-23	0.0	1.2056390148000226E-4	0.0	6.697994526666793E-5	1.4065788506000264E-4
24-25	0.0	1.2056390148000226E-4	0.0	1.0046991790000189E-4	1.540538741133362E-4
26-27	0.0	1.2056390148000226E-4	0.0	2.0093983580000377E-4	1.94241841273337E-4
28-29	0.0	1.2056390148000226E-4	0.0	6.430074745600121E-4	2.1433582485333737E-4
30-31	0.0	1.3395989053333586E-4	0.0	0.0022773181390667095	2.1433582485333737E-4
32-33	0.0	1.4065788506000264E-4	0.0	0.0053583956213334345	2.2773181390667096E-4
34-35	0.0	1.808458522200034E-4	0.0	0.009089178572686837	2.2773181390667096E-4
36-37	0.0	2.2103381938000415E-4	0.0	0.015124071641213618	2.2773181390667096E-4
38-39	0.0	2.7461777559333847E-4	0.0	0.024300324142747125	2.4112780296000453E-4
40-41	0.0	2.9471175917333885E-4	0.0	0.034360711921800645	2.4112780296000453E-4
42-43	0.0	3.0810774822667247E-4	0.0	0.043771394231767485	2.4782579748667134E-4
44-45	0.0	3.0810774822667247E-4	0.0	0.054849877178874365	2.813157701200053E-4
46-47	0.0	3.2820173180667285E-4	0.0	0.06612929996178124	3.2150373728000604E-4
48-49	0.0	3.482957153866732E-4	0.0	0.07723457488699478	3.482957153866732E-4
50-51	0.0	3.482957153866732E-4	0.0	0.08878191745096833	3.616917044400068E-4
52-53	0.0	3.482957153866732E-4	0.0	0.10032926001494188	4.1527566065334117E-4
54-55	0.0	3.683896989666736E-4	0.0	0.11185650859533544	4.420676387600083E-4
56-57	0.0	3.8178568802000717E-4	0.0	0.12446213429452234	4.487656332866751E-4
58-59	0.0	3.88483682546674E-4	0.0	0.13707445798823592	4.554636278133419E-4
60-61	0.0	3.88483682546674E-4	0.0	0.1494657478625695	4.554636278133419E-4
62-63	0.0	3.88483682546674E-4	0.0	0.1618838297150097	5.15745578553343E-4
64-65	0.0	3.88483682546674E-4	0.0	0.17442247546892994	5.224435730800098E-4
66-67	0.0	4.1527566065334117E-4	0.0	0.18821364619933686	5.224435730800098E-4
68-69	0.0	4.1527566065334117E-4	0.0	0.20281527426747048	5.224435730800098E-4
70-71	0.0	4.1527566065334117E-4	0.0	0.21778529203457075	5.224435730800098E-4
72-73	0.0	4.1527566065334117E-4	0.0	0.2325811619439777	5.626315402400106E-4
74-75	0.0	4.1527566065334117E-4	0.0	0.2472430719628513	5.760275292933442E-4
76-77	0.0	4.1527566065334117E-4	0.0	0.2633718427830649	5.894235183466778E-4
78-79	0.0	4.1527566065334117E-4	0.0	0.2798288153350853	6.028195074000113E-4
80-81	0.0	4.1527566065334117E-4	0.0	0.29672785552586556	6.296114855066785E-4
82-83	0.0	4.2867164970667473E-4	0.0	0.31362019772211924	6.564034636133457E-4
84-85	0.0	4.554636278133419E-4	0.0	0.3306531978034329	6.631014581400124E-4
86-87	0.0	4.554636278133419E-4	0.0	0.34822873544140653	6.697994526666792E-4
88-89	0.0	4.554636278133419E-4	0.0	0.3675457516563136	6.697994526666792E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	14140	0.0	36.75037	1
GGTATCA	9015	0.0	31.700924	1
TCAACGC	17340	0.0	29.308043	4
CAACGCA	17525	0.0	28.928535	5
TATCAAC	18365	0.0	27.954885	2
ATCAACG	18490	0.0	27.510523	3
AACGCAG	19005	0.0	26.776558	6
GTACATG	24645	0.0	25.723831	1
TACATGG	25870	0.0	24.01233	2
GTACCTG	6875	0.0	22.585583	1
ACGCAGA	22455	0.0	22.558247	7
ACATGGG	26085	0.0	22.504736	3
GAGTACA	18765	0.0	22.209864	1
AGTACAT	18050	0.0	21.207075	2
CGCAGAG	24020	0.0	21.029196	8
CATGGGG	20560	0.0	19.40476	4
TACCTGG	7955	0.0	18.746164	2
GCAGAGT	27250	0.0	18.257448	9
AGAGTAC	21070	0.0	18.227224	10-11
GTGTACG	1310	0.0	17.81604	1
>>END_MODULE