FastQCFastQC Report
Mon 15 Aug 2016
SRR2921474_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921474_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3865697
Sequences flagged as poor quality0
Sequence length98
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39100.10114605464422069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57850.044.800421
GGTATCA32550.037.053341
TCAACGC72350.035.548944
CAACGCA76100.033.736945
ATCAACG76750.033.5711253
GTACCTG45850.033.4335061
TATCAAC78000.033.2094572
AACGCAG81750.031.6795276
TACCTGG49600.029.8558042
ACGCAGA96500.026.6390467
GTACATG120050.025.5764771
CGCAGAG102850.024.8997558
TACATGG123000.024.377022
ACCTGGG63150.023.086693
ACATGGG128200.022.4226763
GAGTACA91750.021.975821
GCAGAGT119350.021.1113789
AGTACAT87400.020.9824642
CATGGGG98550.018.887384
AGAGTAC103850.017.91001310-11
Produced by FastQC (version 0.11.5)