##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921474_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3865697
Sequences flagged as poor quality	0
Sequence length	98
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.18095650021199	31.0	31.0	34.0	27.0	34.0
2	30.26820053408221	31.0	31.0	34.0	26.0	34.0
3	30.404899814962217	31.0	31.0	34.0	26.0	34.0
4	33.915496998342086	37.0	35.0	37.0	30.0	37.0
5	33.88952729611245	37.0	35.0	37.0	30.0	37.0
6	33.98774089122867	37.0	35.0	37.0	30.0	37.0
7	33.863098427010705	37.0	35.0	37.0	30.0	37.0
8	33.83834506429242	37.0	35.0	37.0	30.0	37.0
9	35.264567554052995	39.0	35.0	39.0	30.0	39.0
10-11	35.129121087348544	39.0	35.0	39.0	29.0	39.0
12-13	34.95448453409566	39.0	35.0	39.0	28.0	39.0
14-15	35.93487940208454	39.5	36.0	41.0	27.0	41.0
16-17	35.77792555909064	39.0	36.0	41.0	27.0	41.0
18-19	35.575368555786966	39.0	36.0	40.5	27.0	41.0
20-21	35.45027235709369	39.0	36.0	40.0	27.0	41.0
22-23	35.48248452995669	39.0	36.0	40.5	27.0	41.0
24-25	35.33319165987402	39.0	35.5	41.0	26.0	41.0
26-27	35.046434575705234	39.0	35.0	40.0	24.5	41.0
28-29	34.79529629456215	39.0	34.5	40.0	24.0	41.0
30-31	34.51855331651704	38.0	34.0	40.0	23.5	41.0
32-33	34.230225622960106	38.0	34.0	40.0	21.5	41.0
34-35	33.79553842424794	38.0	33.0	40.0	17.5	41.0
36-37	33.395326121007415	38.0	33.0	40.0	15.5	41.0
38-39	33.13388607539598	38.0	32.5	40.0	14.0	41.0
40-41	32.84432496908061	38.0	32.0	40.0	9.0	41.0
42-43	32.5315906808009	37.5	31.0	40.0	9.0	41.0
44-45	32.188682920570336	37.0	31.0	40.0	8.0	41.0
46-47	31.87943131600847	37.0	30.5	40.0	7.0	41.0
48-49	31.64683768541611	37.0	30.0	39.0	3.5	40.0
50-51	30.912643049882078	35.5	29.5	38.5	2.0	39.5
52-53	31.458386029737973	36.5	30.5	39.0	2.0	40.0
54-55	31.873805422411536	37.0	31.0	39.5	2.0	40.5
56-57	31.70604705956002	36.5	31.0	39.5	2.0	41.0
58-59	31.357759157016183	36.0	31.0	39.0	2.0	40.5
60-61	30.88370324937521	35.5	30.0	39.0	2.0	40.0
62-63	30.372314746861953	35.0	29.0	38.5	2.0	40.0
64-65	29.80831050648822	35.0	28.5	38.0	2.0	40.0
66-67	29.301526348288547	34.0	28.0	37.5	2.0	40.0
68-69	28.769267094653305	34.0	27.5	37.0	2.0	39.0
70-71	28.23002164939466	34.0	26.0	36.0	2.0	39.0
72-73	27.66197829265977	33.5	26.0	36.0	2.0	38.5
74-75	27.048737911947057	33.0	25.0	35.0	2.0	37.5
76-77	26.44159850086543	32.5	23.5	35.0	2.0	37.0
78-79	25.858731556042805	32.0	21.5	35.0	2.0	36.0
80-81	25.252834223685923	31.5	19.0	34.5	2.0	36.0
82-83	24.599873580365973	31.0	16.5	34.0	2.0	35.0
84-85	23.947049652365408	31.0	8.5	34.0	2.0	35.0
86-87	23.288754783419392	30.0	4.0	34.0	2.0	35.0
88-89	22.59851755065128	30.0	2.0	34.0	2.0	35.0
90-91	21.821625957750957	29.0	2.0	34.0	2.0	35.0
92-93	20.960607750685064	29.0	2.0	34.0	2.0	35.0
94-95	20.01106385213326	27.5	2.0	33.0	2.0	34.0
96-97	19.083392593884106	26.0	2.0	33.0	2.0	34.0
98	18.44559105382548	25.0	2.0	32.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	132173.0
3	23574.0
4	17846.0
5	15263.0
6	14485.0
7	15141.0
8	17236.0
9	19831.0
10	22207.0
11	24444.0
12	26540.0
13	29714.0
14	25195.0
15	25850.0
16	28313.0
17	32559.0
18	36191.0
19	35722.0
20	37326.0
21	40776.0
22	44478.0
23	48902.0
24	54590.0
25	61591.0
26	69415.0
27	79068.0
28	90847.0
29	104622.0
30	124533.0
31	149150.0
32	183482.0
33	234083.0
34	309998.0
35	419967.0
36	541269.0
37	522168.0
38	199518.0
39	7630.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.673827249372025	16.598734043977093	12.666655404483476	25.060783302167405
2	16.58306140703226	18.329946332514794	38.322948026187746	26.7640442342652
3	20.824439138340484	21.74622587178641	26.87717663502417	30.552158354848935
4	14.175650307213719	14.030785301883194	33.74192494714123	38.051639443761864
5	16.156179429753582	35.00990895564578	32.12603528913546	16.707876325465186
6	36.128340861732006	32.09391299845981	16.31489846368896	15.462847676119228
7	32.31815491494991	29.346998214552368	18.748830556387304	19.58601631411042
8	28.61676292531774	33.06232396206507	18.485904287110724	19.835008825506463
9	27.13352336965549	16.525812462194967	18.49563259690378	37.84503157124577
10-11	27.29569835373214	24.530233278954057	25.389646652841368	22.78442171447244
12-13	27.301698383757504	21.516715746609087	26.235802335787007	24.945783533846402
14-15	24.248732801146254	23.15888292716435	23.690108417087753	28.90227585460165
16-17	24.94517026131218	26.23442264938436	24.20680248383826	24.6136046054652
18-19	24.917093359509725	25.45170773120195	25.649169510027413	23.98202939926091
20-21	25.35086355509718	24.702037018362383	25.662105079063473	24.28499434747696
22-23	25.130482586180914	24.330082360173368	25.574094895202208	24.96534015844351
24-25	24.853891811146145	24.579556722646384	25.667564894583233	24.89898657162424
26-27	24.85686712238416	24.806509929364005	25.64328077759791	24.693342170653924
28-29	24.846119978712405	24.843103675268654	25.618539624971216	24.692236721047728
30-31	24.975242642807114	24.843186148925145	25.477986529305824	24.703584678961914
32-33	24.84462036338832	24.849226504668508	25.38127463832734	24.924878493615836
34-35	25.00495394197434	24.84868726381124	25.537577370445003	24.608781423769415
36-37	25.05029800076916	24.81647850874025	25.458603629368902	24.674619861121684
38-39	24.984777610020384	24.879167198360914	25.39997390633258	24.736081285286122
40-41	25.126328630947402	24.789157453164854	25.43381750691297	24.650696408974774
42-43	25.077118051410647	24.74245652690101	25.345031922621096	24.835393499067248
44-45	24.9583425862025	24.912810668874528	25.347821202843523	24.781025542079448
46-47	25.079705898106454	24.81302720166905	25.38103085428186	24.72623604594263
48-49	25.113589404379837	24.913793204303378	25.154419584239317	24.818197807077468
50-51	24.89361940018113	25.187402768846823	25.107823709697257	24.811154121274797
52-53	24.97476068681119	25.041757580023816	25.185614183511635	24.79786754965336
54-55	24.99008818652316	24.962863218534935	25.26055582120025	24.786492773741656
56-57	24.88347809263321	25.1167624469139	25.274686411192864	24.72507304926003
58-59	24.971908578737875	24.99200544929375	25.279406297366087	24.756679674602285
60-61	25.044183657364155	24.935937922123916	25.263717345241492	24.756161075270438
62-63	24.84592917156268	25.108269238195707	25.249672302461867	24.796129287779745
64-65	24.93634408048392	24.941012853170218	25.27321098547908	24.849432080866784
66-67	24.930200890571985	25.013492825095135	25.245300145601533	24.811006138731347
68-69	24.93051899563526	25.019610708740704	25.279656573029786	24.770213722594253
70-71	24.829841091449804	24.892779159507132	25.340610219853083	24.93676952918998
72-73	24.975244343944905	24.96504453289478	25.135842642772342	24.923868480387974
74-75	24.816169664717734	25.12788522008391	25.176198208223393	24.879746906974958
76-77	25.00754553860854	24.941958144900585	25.090556207863706	24.959940108627173
78-79	24.97259355283955	24.865929921084504	25.265054577740635	24.896421948335306
80-81	24.910543760642476	25.07771841671518	25.183456256766135	24.828281565876207
82-83	25.07433924998051	24.968229423841574	25.074378232282545	24.88305309389537
84-85	25.015068167871863	24.988932690494117	25.016302198861368	24.97969694277266
86-87	25.035670768935	24.945636501095063	25.193552242218292	24.82514048775165
88-89	24.919111554395197	24.803429480386995	25.185961240333242	25.091497724884558
90-91	24.972003591854723	24.91489351751289	25.149803518479448	24.963299372152942
92-93	25.013637039745852	24.92882043341039	25.20858922404302	24.84895330280074
94-95	25.119527771758083	24.781627957857626	25.14855223285673	24.950292037527564
96-97	25.080524037300435	24.738512049082605	25.14557205162113	25.035391861995837
98	25.00921047640377	24.84129141012004	25.07112462385142	25.078373489624767
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	395.0
1	295.5
2	319.0
3	449.0
4	394.5
5	344.5
6	553.5
7	1095.0
8	1616.5
9	1646.5
10	1413.0
11	1366.0
12	1482.0
13	1715.0
14	2069.5
15	2391.5
16	2609.0
17	2801.5
18	2889.0
19	2878.0
20	2787.0
21	2607.0
22	2502.5
23	2557.0
24	2780.5
25	3213.0
26	3797.0
27	4887.0
28	6586.5
29	8775.0
30	11884.5
31	15934.0
32	20867.0
33	26263.0
34	32288.0
35	39491.0
36	48094.0
37	57940.5
38	68207.0
39	78815.5
40	89525.5
41	100931.5
42	112571.0
43	123791.0
44	135182.0
45	146687.0
46	158017.5
47	168497.5
48	175266.5
49	177831.5
50	178608.0
51	178636.0
52	180233.5
53	181438.0
54	178953.0
55	172740.5
56	162249.5
57	150556.5
58	138466.0
59	124522.0
60	110151.5
61	94150.5
62	77104.0
63	60084.5
64	45183.0
65	34859.5
66	27065.0
67	20931.5
68	16427.5
69	12912.5
70	10028.0
71	7786.0
72	6208.0
73	4882.0
74	3839.0
75	3008.0
76	2202.5
77	1463.5
78	922.5
79	587.0
80	354.0
81	225.5
82	143.5
83	82.0
84	46.5
85	29.0
86	23.0
87	15.5
88	11.5
89	11.0
90	10.5
91	6.0
92	3.5
93	6.0
94	7.5
95	10.0
96	7.0
97	5.0
98	4.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.465944433823965
2	0.04927959951336072
3	0.057479931820833344
4	0.05432396796748426
5	0.06531810434185607
6	0.06588721257770591
7	0.04345917437398741
8	0.01973770836151928
9	0.01218408995842147
10-11	0.014499325736083297
12-13	0.010463830972784468
14-15	0.014939091191058173
16-17	0.014227705896245878
18-19	0.05599248984077128
20-21	0.08439616452091304
22-23	0.10034412940279593
24-25	0.0703754070740671
26-27	0.08509461553763784
28-29	0.08675020313283736
30-31	0.05688495502880851
32-33	0.033396306021915324
34-35	0.3939263734327858
36-37	0.6168227877146087
38-39	0.5652926238140237
40-41	0.5601965182475502
42-43	0.5333578912159954
44-45	0.5184834714153748
46-47	0.5091707911923774
48-49	0.49868109166341806
50-51	0.5131286802871513
52-53	0.5944982237355902
54-55	0.5639086560586616
56-57	0.5219110551085613
58-59	0.4999615851940802
60-61	0.5126630462760015
62-63	0.5252998359674853
64-65	0.5433302196214551
66-67	0.5687848788976477
68-69	0.5231010086926109
70-71	0.4724633099800631
72-73	0.4549373631715057
74-75	0.4355100774840863
76-77	0.45002233749825715
78-79	0.4436457384011215
80-81	0.44047684026968487
82-83	0.46038269424634165
84-85	0.4274261536794012
86-87	0.4295991123981005
88-89	0.45659295076670525
90-91	0.43948090085694763
92-93	0.4347081522426615
94-95	0.4454306687771959
96-97	0.439804257809135
98	0.43508324630719897
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3865697.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.16676331493531
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.10346539413885	43.505887936331064
2	13.143702858189025	15.027659007518611
3	4.553187996981075	7.8087306165546435
4	2.0200200537793926	4.619120332233177
5	1.0748613323489375	3.0723171691383864
6	0.6831883858944119	2.343340125356312
7	0.43769487125063067	1.751511937626212
8	0.32979031254471725	1.5082435792641913
9	0.23848990930958316	1.2270326578651192
>10	1.3110290913199536	13.456184917544364
>50	0.07824171804301698	3.0229693361807786
>100	0.025919686665622096	2.3446028556890863
>500	2.722596978665241E-4	0.10264593503473333
>1k	1.361298368640986E-4	0.20975359366332985
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3910	0.10114605464422069	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.5868556174992505E-5	0.0	0.0	0.0
3	0.0	2.5868556174992505E-5	0.0	0.0	0.0
4	0.0	2.5868556174992505E-5	0.0	0.0	0.0
5	0.0	2.5868556174992505E-5	0.0	0.0	0.0
6	0.0	2.5868556174992505E-5	0.0	0.0	2.5868556174992505E-5
7	0.0	2.5868556174992505E-5	0.0	0.0	7.760566852497751E-5
8	0.0	2.5868556174992505E-5	0.0	0.0	7.760566852497751E-5
9	0.0	2.5868556174992505E-5	0.0	0.0	7.760566852497751E-5
10-11	2.5868556174992505E-5	2.5868556174992505E-5	0.0	0.0	7.760566852497751E-5
12-13	2.5868556174992505E-5	5.173711234998501E-5	0.0	2.5868556174992505E-5	1.8107989322494754E-4
14-15	2.5868556174992505E-5	7.760566852497752E-5	0.0	2.5868556174992505E-5	2.0694844939994004E-4
16-17	2.5868556174992505E-5	1.0347422469997002E-4	0.0	2.5868556174992505E-5	2.0694844939994004E-4
18-19	2.5868556174992505E-5	1.0347422469997002E-4	0.0	3.880283426248876E-5	2.198827274874363E-4
20-21	2.5868556174992505E-5	1.0347422469997002E-4	0.0	5.173711234998501E-5	2.3281700557493254E-4
22-23	2.5868556174992505E-5	1.0347422469997002E-4	0.0	5.173711234998501E-5	2.3281700557493254E-4
24-25	2.5868556174992505E-5	1.0347422469997002E-4	0.0	9.053994661247376E-5	2.8455411792491757E-4
26-27	2.5868556174992505E-5	1.0347422469997002E-4	0.0	2.198827274874363E-4	3.6215978644989503E-4
28-29	2.5868556174992505E-5	1.0347422469997002E-4	0.0	7.631224071622789E-4	3.8802834262488756E-4
30-31	2.5868556174992505E-5	1.0347422469997002E-4	0.0	0.002198827274874363	3.8802834262488756E-4
32-33	2.5868556174992505E-5	1.0347422469997002E-4	0.0	0.005367725406310945	3.8802834262488756E-4
34-35	5.173711234998501E-5	1.4227705896245878E-4	0.0	0.008989323270809896	3.8802834262488756E-4
36-37	5.173711234998501E-5	1.6814561513745128E-4	0.0	0.01642653317112024	3.8802834262488756E-4
38-39	5.173711234998501E-5	1.8107989322494754E-4	0.0	0.026942101256254694	4.138968987998801E-4
40-41	5.173711234998501E-5	1.8107989322494754E-4	0.0	0.038388937363688876	4.268311768873763E-4
42-43	5.173711234998501E-5	2.0694844939994004E-4	0.0	0.04881396550221086	4.3976545497487256E-4
44-45	5.173711234998501E-5	2.0694844939994004E-4	0.0	0.06037721011243251	4.526997330623688E-4
46-47	1.0347422469997002E-4	2.0694844939994004E-4	0.0	0.07260010290511647	5.044368454123539E-4
48-49	1.0347422469997002E-4	2.0694844939994004E-4	0.0	0.08623283200933751	5.432396796748426E-4
50-51	1.0347422469997002E-4	2.0694844939994004E-4	0.0	0.1004217350713209	5.432396796748426E-4
52-53	1.0347422469997002E-4	2.0694844939994004E-4	0.0	0.11352415877395461	5.820425139373313E-4
54-55	1.0347422469997002E-4	2.0694844939994004E-4	1.2934278087496253E-5	0.1287219355267627	6.208453481998201E-4
56-57	1.0347422469997002E-4	2.0694844939994004E-4	2.5868556174992505E-5	0.14335060404372096	6.337796262873164E-4
58-59	1.0347422469997002E-4	2.0694844939994004E-4	2.5868556174992505E-5	0.1577076527208418	6.596481824623088E-4
60-61	1.0347422469997002E-4	2.3281700557493254E-4	2.5868556174992505E-5	0.17350040626567473	6.855167386373014E-4
62-63	1.0347422469997002E-4	2.3281700557493254E-4	2.5868556174992505E-5	0.18886632863362027	7.243195728997902E-4
64-65	1.2934278087496252E-4	2.3281700557493254E-4	2.5868556174992505E-5	0.20381835410276594	7.243195728997902E-4
66-67	1.2934278087496252E-4	2.3281700557493254E-4	2.5868556174992505E-5	0.22002500454639876	7.501881290747827E-4
68-69	1.2934278087496252E-4	2.3281700557493254E-4	2.5868556174992505E-5	0.23656794622030647	7.760566852497751E-4
70-71	1.2934278087496252E-4	2.3281700557493254E-4	2.5868556174992505E-5	0.2539645502479889	8.019252414247676E-4
72-73	1.2934278087496252E-4	2.3281700557493254E-4	2.5868556174992505E-5	0.2705850975904216	8.407280756872564E-4
74-75	1.2934278087496252E-4	2.3281700557493254E-4	2.5868556174992505E-5	0.28910698381171623	8.536623537747527E-4
76-77	1.2934278087496252E-4	2.3281700557493254E-4	2.5868556174992505E-5	0.3064647850051362	8.536623537747527E-4
78-79	1.2934278087496252E-4	2.3281700557493254E-4	2.5868556174992505E-5	0.32545230523758073	8.536623537747527E-4
80-81	1.2934278087496252E-4	2.3281700557493254E-4	2.5868556174992505E-5	0.3445303654166377	8.795309099497452E-4
82-83	1.2934278087496252E-4	2.3281700557493254E-4	2.5868556174992505E-5	0.3633109371996822	9.571365784747227E-4
84-85	1.2934278087496252E-4	2.3281700557493254E-4	2.5868556174992505E-5	0.3823113917102142	9.571365784747227E-4
86	1.2934278087496252E-4	2.3281700557493254E-4	2.5868556174992505E-5	0.396720177499685	9.571365784747227E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5785	0.0	44.80042	1
GGTATCA	3255	0.0	37.05334	1
TCAACGC	7235	0.0	35.54894	4
CAACGCA	7610	0.0	33.73694	5
ATCAACG	7675	0.0	33.571125	3
GTACCTG	4585	0.0	33.433506	1
TATCAAC	7800	0.0	33.209457	2
AACGCAG	8175	0.0	31.679527	6
TACCTGG	4960	0.0	29.855804	2
ACGCAGA	9650	0.0	26.639046	7
GTACATG	12005	0.0	25.576477	1
CGCAGAG	10285	0.0	24.899755	8
TACATGG	12300	0.0	24.37702	2
ACCTGGG	6315	0.0	23.08669	3
ACATGGG	12820	0.0	22.422676	3
GAGTACA	9175	0.0	21.97582	1
GCAGAGT	11935	0.0	21.111378	9
AGTACAT	8740	0.0	20.982464	2
CATGGGG	9855	0.0	18.88738	4
AGAGTAC	10385	0.0	17.910013	10-11
>>END_MODULE