FastQCFastQC Report
Mon 15 Aug 2016
SRR2921474_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921474_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3865697
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57650.045.6955761
GGTATCA33050.037.4757421
TCAACGC73700.034.932914
CAACGCA76450.033.659765
GTACCTG45900.033.3150671
TATCAAC78800.033.0324252
ATCAACG77950.033.0282943
AACGCAG84650.030.7364736
TACCTGG51600.029.7330112
ACGCAGA102450.024.9797747
GTACATG118300.024.7247561
CGCAGAG108250.023.4659188
ACCTGGG64100.023.1947293
TACATGG123300.023.1524772
ACATGGG125650.021.7752423
GAGTACA93400.019.6364171
GCAGAGT128300.019.5762489
CATGGGG96350.019.5722944
GTATAGC8900.018.7338221
AGTACAT89300.018.669892
Produced by FastQC (version 0.11.5)