##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921474_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3865697
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.948026190361013	33.0	31.0	34.0	30.0	34.0
2	32.06201960474398	34.0	31.0	34.0	30.0	34.0
3	32.189622207845055	34.0	31.0	34.0	30.0	34.0
4	35.73249481270777	37.0	35.0	37.0	35.0	37.0
5	35.53459259740223	37.0	35.0	37.0	33.0	37.0
6	35.57375241773993	37.0	35.0	37.0	33.0	37.0
7	35.5070017127571	37.0	35.0	37.0	33.0	37.0
8	35.4556394357861	37.0	35.0	37.0	33.0	37.0
9	37.081971763436194	39.0	37.0	39.0	34.0	39.0
10-11	36.995048758348105	39.0	37.0	39.0	33.0	39.0
12-13	36.873962444547516	39.0	37.0	39.0	33.0	39.0
14-15	38.1695127683313	40.0	38.0	41.0	33.0	41.0
16-17	38.065805855968534	40.0	38.0	41.0	33.0	41.0
18-19	37.95386355940468	40.0	38.0	41.0	33.0	41.0
20-21	37.835094680209025	40.0	38.0	41.0	33.0	41.0
22-23	37.79702302068682	40.0	38.0	41.0	32.5	41.0
24-25	37.85150349341917	40.0	38.0	41.0	33.0	41.0
26-27	37.735466333755596	40.0	38.0	41.0	33.0	41.0
28-29	37.59720420405428	40.0	38.0	41.0	32.0	41.0
30-31	37.45424951826281	40.0	37.5	41.0	32.0	41.0
32-33	37.290053643624944	40.0	37.0	41.0	31.5	41.0
34-35	37.13677520509238	40.0	37.0	41.0	31.0	41.0
36-37	37.03372690616983	40.0	37.0	41.0	31.0	41.0
38-39	36.9985073843087	40.0	37.0	41.0	31.0	41.0
40-41	36.82991954620344	40.0	36.5	41.0	30.5	41.0
42-43	36.581757571791066	39.0	36.0	41.0	30.0	41.0
44-45	36.36298124245123	39.0	35.5	41.0	30.0	41.0
46-47	36.0839796807665	39.0	35.0	40.0	29.5	41.0
48-49	35.7400921489708	39.0	35.0	40.0	28.5	41.0
50-51	35.4509667984842	38.5	35.0	40.0	28.0	41.0
52-53	35.12492145659631	38.0	34.0	40.0	27.0	41.0
54-55	34.78240521696346	38.0	34.0	40.0	26.0	41.0
56-57	34.385440969636264	37.5	33.5	40.0	26.0	41.0
58-59	33.9763889927224	37.0	33.0	40.0	25.0	41.0
60-61	33.536839410848806	36.0	33.0	39.0	23.5	40.5
62-63	33.0756793664894	36.0	32.0	39.0	22.5	40.0
64-65	32.544955023634806	35.0	31.5	38.5	21.5	40.0
66-67	32.00134309543661	35.0	31.0	38.0	20.0	40.0
68-69	31.486976216708136	35.0	31.0	37.0	18.0	39.5
70-71	30.923718413522842	34.0	30.0	36.5	16.0	39.0
72-73	30.364849857606533	34.0	29.0	36.0	12.5	39.0
74-75	29.84134517009481	34.0	29.0	35.5	8.0	38.0
76-77	28.594852622955187	32.0	27.5	34.5	7.5	36.0
78-79	29.53515497981347	34.0	29.0	35.0	7.0	37.0
80-81	29.56184926030157	34.0	29.0	35.0	7.0	36.0
82-83	29.219023374051304	34.0	29.0	35.0	2.0	36.0
84-85	28.713722001491583	34.0	29.0	35.0	2.0	35.5
86-87	28.17894276245655	33.0	27.5	35.0	2.0	35.0
88-89	27.616533706599355	33.0	26.5	35.0	2.0	35.0
90-91	27.115735661641356	32.5	25.5	34.5	2.0	35.0
92-93	26.646025283409436	32.0	25.0	34.0	2.0	35.0
94-95	26.111457261135573	32.0	24.5	34.0	2.0	35.0
96-97	25.297470029337532	32.0	21.5	34.0	2.0	35.0
98-99	24.23832726672577	31.0	10.0	34.0	2.0	35.0
100-101	20.64595078196765	27.0	2.0	32.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	23141.0
3	12415.0
4	7673.0
5	5936.0
6	5163.0
7	5404.0
8	6254.0
9	7552.0
10	8926.0
11	10582.0
12	12144.0
13	14871.0
14	16397.0
15	18849.0
16	21161.0
17	24568.0
18	25001.0
19	22929.0
20	23274.0
21	24885.0
22	26623.0
23	29789.0
24	33779.0
25	39317.0
26	46976.0
27	56447.0
28	68441.0
29	83296.0
30	102393.0
31	127261.0
32	164436.0
33	217072.0
34	306051.0
35	455152.0
36	686528.0
37	798062.0
38	318241.0
39	8708.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.87118408508602	17.08627913168166	13.04978937639116	24.992747406841154
2	16.70627573759661	18.634905943223178	37.923743117993986	26.735075201186227
3	20.843420496425537	21.887949576170996	27.004993436768686	30.26363649063478
4	14.435963685740955	14.352591224381928	33.60521055882068	37.60623453105644
5	16.216008567703483	34.999689575960765	32.14394362699448	16.640358229341278
6	36.31975812609852	32.16482836634163	16.150933042035426	15.364480465524426
7	32.35893036322461	29.33882393386841	18.682064302881503	19.620181400025473
8	28.62135892160506	33.11232331131779	18.43844559276892	19.827872174308233
9	27.01574701414686	16.493364152259026	18.56129093564715	37.92959789794696
10-11	27.278266081996268	24.52731159074108	25.45187020731459	22.74255211994806
12-13	27.23134027879373	21.41745393248826	26.354863224835867	24.996342563882138
14-15	24.190599750024884	23.11343141907926	23.77917751533741	28.91679131555845
16-17	24.898652866903408	26.21572890943873	24.339187967862372	24.546430255795492
18-19	24.83952710571181	25.44373951271724	25.76006597995344	23.956667401617512
20-21	25.24496463206885	24.66742578155475	25.76236771392349	24.325241872452917
22-23	25.061708969681472	24.31139311395702	25.708702090470602	24.918195825890905
24-25	24.782392114698123	24.522882565362586	25.803493681433494	24.891231638505797
26-27	24.733249096319337	24.814247552031578	25.77183015758411	24.68067319406498
28-29	24.742152272017485	24.914167193432178	25.728741162823976	24.614939371726365
30-31	24.917641480160032	24.797439238238823	25.569317941203213	24.715601340397924
32-33	24.755681829205905	24.780723851556168	25.54045690049353	24.923137418744393
34-35	24.861915329455147	24.815192909782265	25.719527850028793	24.60336391073379
36-37	24.856067455133417	24.838720651918994	25.589574635946743	24.715637257000843
38-39	24.84631199896405	24.84913214031005	25.610311575166662	24.694244285559233
40-41	24.977813138761675	24.778403063468655	25.58214960283578	24.661634194933892
42-43	24.95267820532886	24.705404527939713	25.53233623632312	24.80958103040831
44-45	24.782630012238926	24.85650857740361	25.544351523811702	24.816509886545756
46-47	24.881610582520956	24.84779242742422	25.560781082946853	24.70981590710797
48-49	24.96295841188435	24.91384556681229	25.348798856577424	24.77439716472593
50-51	24.83853254799138	25.158265978425305	25.25098582749684	24.752215646086473
52-53	24.961315852138405	25.01075188427985	25.250611062728527	24.777321200853216
54-55	24.92294088870918	24.914494459892918	25.351186471019954	24.81137818037795
56-57	24.807980429208577	25.09275184569156	25.338305105616747	24.76096261948312
58-59	24.933597799592437	24.962622691282636	25.32957285939807	24.774206649726857
60-61	25.008925111331067	24.92339926188036	25.334471780867563	24.733203845921018
62-63	24.808890373389005	25.054070056475542	25.31799428453081	24.819045285604645
64-65	24.881306620596664	24.918353028466232	25.33681962797335	24.863520722963752
66-67	24.91598598015235	24.982105130224966	25.272278918782394	24.82962997084029
68-69	24.85912272943346	25.01722261113033	25.320117964047288	24.80353669538892
70-71	24.786950128627417	24.884124076850725	25.418748957857268	24.91017683666459
72-73	24.99003746946549	24.966412039912225	25.18732145075146	24.856229039870822
74-75	24.773421716420113	25.124631643677986	25.192621951629828	24.909324688272072
76-77	24.89977813807746	24.94244588823774	25.21625115880109	24.941524814883714
78-79	24.82180338546386	24.97403483629262	25.356199072813258	24.847962705430266
80-81	24.79520689951423	25.15272793518663	25.285892617407985	24.766172547891156
82-83	24.942808134195754	24.9985703650783	25.19786535452488	24.860756146201066
84-85	24.87043133151649	25.103630354838717	25.092646999085883	24.93329131455891
86-87	24.832802002374642	25.043525801654948	25.256619321040226	24.867052874930188
88-89	24.739303390993168	24.946511104211556	25.252284524520903	25.061900980274377
90-91	24.786206896551725	25.08285575522098	25.22243160110086	24.908505747126437
92-93	24.84272123069278	25.094142096981244	25.288573025798172	24.774563646527806
94-95	24.904598605285354	24.99325745201925	25.18640800635695	24.915735936338447
96-97	24.89514246990609	24.885712859169214	25.2886637969951	24.930480873929596
98-99	24.77075826634302	24.971803593384468	25.268792376265992	24.988645764006524
100-101	25.356560585419057	24.423514035538865	25.334609440363515	24.885315938678566
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	88.0
1	81.0
2	187.5
3	297.0
4	213.5
5	163.5
6	357.5
7	839.0
8	1292.0
9	1290.0
10	944.5
11	709.5
12	683.5
13	711.5
14	792.0
15	878.0
16	970.5
17	1059.0
18	1128.5
19	1258.5
20	1349.5
21	1446.0
22	1630.0
23	1827.0
24	2066.5
25	2492.5
26	3114.0
27	3912.5
28	5131.5
29	7071.0
30	9835.5
31	13235.0
32	17438.0
33	22219.5
34	27644.0
35	33882.5
36	41671.5
37	50719.5
38	60298.0
39	70744.5
40	81625.5
41	92075.5
42	102652.0
43	113462.0
44	125394.0
45	137247.0
46	148218.0
47	158769.0
48	168803.0
49	175133.0
50	176354.5
51	176987.5
52	178437.5
53	179406.0
54	178015.0
55	172228.0
56	161828.0
57	148651.0
58	136069.0
59	123619.0
60	109025.0
61	94371.0
62	78220.5
63	60157.0
64	44852.0
65	33992.0
66	26243.5
67	20836.0
68	16393.0
69	12822.0
70	10158.5
71	7968.0
72	6090.0
73	4884.5
74	3931.5
75	3038.0
76	2217.5
77	1427.5
78	971.5
79	621.0
80	386.0
81	235.5
82	128.0
83	61.5
84	31.0
85	10.0
86	4.5
87	4.5
88	3.0
89	3.5
90	3.0
91	1.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3079134241509358
2	0.0
3	0.005768688027023328
4	0.05978223332040768
5	4.397654549748726E-4
6	0.06536984145420606
7	0.07380299076725362
8	0.10430201849756977
9	0.09229900843237325
10-11	0.09550670939807232
12-13	0.09576539495982225
14-15	0.06499474738966866
16-17	0.03568567324340216
18-19	0.03862175436926381
20-21	0.02867529451997919
22-23	0.041156872874413074
24-25	0.03342217457809032
26-27	0.02091472766748144
28-29	0.023695597456293134
30-31	0.0033241094684865365
32-33	0.005031434176036042
34-35	0.00814859519512264
36-37	0.011162281989509266
38-39	0.016620547342432682
40-41	0.020617239271469027
42-43	0.0036733349768489354
44-45	0.014758011297833225
46-47	0.023514517563068188
48-49	0.0028326069011616792
50-51	0.00748894701266033
52-53	0.0024704471147117845
54-55	0.004087231875648816
56-57	0.0036345321425864468
58-59	0.001280493530662129
60-61	0.005147842678823508
62-63	0.014809748410183208
64-65	0.007113852948122939
66-67	0.03779396057166405
68-69	0.05992451037937014
70-71	0.011990075787109025
72-73	0.03148203286496588
74-75	0.049434810850410675
76-77	0.2974366589000638
78-79	0.0731821454190538
80-81	0.03386194003306519
82-83	0.02778282933194195
84-85	0.01960836558064432
86-87	0.11592993449822891
88-89	0.5183799971906748
90-91	0.131205316919562
92-93	0.04803790881696108
94-95	0.008239135141735113
96-97	0.0055358710214483955
98-99	0.010334488191909505
100-101	0.007631224071622788
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3865697.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.1951091072291
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.65166371448136	37.39866405291842
2	15.407440512943914	16.08386077272499
3	5.409866576235099	8.471057286064287
4	2.4177528983472394	5.047795052942141
5	1.333820264842755	3.480944712645041
6	0.8041351798319493	2.5183154068973925
7	0.5609644084560579	2.0495718953245317
8	0.39265001015539563	1.639552809681239
9	0.2922583190845255	1.3729009366900964
>10	1.593042075738622	15.081787292529961
>50	0.10059551814689392	3.5834326137011185
>100	0.03521547008773365	2.9163905540944572
>500	3.967011113832374E-4	0.1254257320972149
>1k	1.9835053708995256E-4	0.23030088168912274
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.760566852497751E-5
2	0.0	0.0	0.0	0.0	7.760566852497751E-5
3	0.0	0.0	0.0	0.0	7.760566852497751E-5
4	0.0	0.0	0.0	0.0	7.760566852497751E-5
5	0.0	5.173711234998501E-5	0.0	0.0	7.760566852497751E-5
6	0.0	5.173711234998501E-5	0.0	0.0	7.760566852497751E-5
7	0.0	5.173711234998501E-5	0.0	0.0	7.760566852497751E-5
8	0.0	5.173711234998501E-5	0.0	0.0	1.0347422469997002E-4
9	0.0	5.173711234998501E-5	0.0	0.0	1.0347422469997002E-4
10-11	0.0	5.173711234998501E-5	0.0	0.0	1.0347422469997002E-4
12-13	0.0	5.173711234998501E-5	0.0	0.0	1.6814561513745128E-4
14-15	0.0	5.173711234998501E-5	0.0	0.0	2.198827274874363E-4
16-17	0.0	5.173711234998501E-5	0.0	0.0	3.1042267409991004E-4
18-19	0.0	5.173711234998501E-5	0.0	1.2934278087496253E-5	3.3629123027490256E-4
20-21	0.0	5.173711234998501E-5	0.0	2.5868556174992505E-5	3.492255083623988E-4
22-23	0.0	5.173711234998501E-5	0.0	2.5868556174992505E-5	3.8802834262488756E-4
24-25	0.0	5.173711234998501E-5	0.0	9.053994661247376E-5	4.3976545497487256E-4
26-27	0.0	5.173711234998501E-5	0.0	2.198827274874363E-4	5.173711234998501E-4
28-29	0.0	5.173711234998501E-5	0.0	7.372538509872864E-4	5.432396796748426E-4
30-31	0.0	5.173711234998501E-5	0.0	0.002134155884436882	5.561739577623389E-4
32-33	0.0	5.173711234998501E-5	0.0	0.0052771854596984705	6.467139043748126E-4
34-35	0.0	6.467139043748126E-5	0.0	0.008847046211847436	6.725824605498051E-4
36-37	0.0	7.760566852497751E-5	0.0	0.016374796058770257	6.725824605498051E-4
38-39	0.0	7.760566852497751E-5	0.0	0.027110246871392144	6.725824605498051E-4
40-41	0.0	7.760566852497751E-5	0.0	0.038505345866476345	6.725824605498051E-4
42-43	0.0	7.760566852497751E-5	0.0	0.04881396550221086	7.243195728997902E-4
44-45	0.0	7.760566852497751E-5	0.0	0.06046775005904498	7.501881290747827E-4
46-47	0.0	7.760566852497751E-5	0.0	0.07254836579276647	8.14859519512264E-4
48-49	0.0	7.760566852497751E-5	0.0	0.08611642350655005	8.795309099497451E-4
50-51	0.0	7.760566852497751E-5	0.0	0.10021478662192096	8.795309099497451E-4
52-53	0.0	7.760566852497751E-5	0.0	0.11313613043132972	8.924651880372414E-4
54-55	0.0	7.760566852497751E-5	0.0	0.1280105502319504	9.053994661247376E-4
56-57	0.0	7.760566852497751E-5	0.0	0.14235466463098376	9.053994661247376E-4
58-59	0.0	7.760566852497751E-5	0.0	0.15646596202444218	9.183337442122338E-4
60-61	0.0	7.760566852497751E-5	0.0	0.17203883284178767	9.312680222997302E-4
62-63	0.0	7.760566852497751E-5	0.0	0.18722367531650824	9.312680222997302E-4
64-65	0.0	7.760566852497751E-5	0.0	0.2019299495019915	9.312680222997302E-4
66-67	0.0	7.760566852497751E-5	0.0	0.21789084866196184	9.442023003872265E-4
68-69	0.0	1.0347422469997002E-4	0.0	0.2340069591589822	9.571365784747227E-4
70-71	0.0	1.0347422469997002E-4	0.0	0.25101553484403977	0.0010476765250871964
72-73	0.0	1.0347422469997002E-4	0.0	0.2675584765179475	0.0011123479155246778
74-75	0.0	1.0347422469997002E-4	0.0	0.2858087428994047	0.0011770193059621589
76-77	0.0	1.0347422469997002E-4	0.0	0.30270091008167477	0.0012028878621371515
78-79	0.0	1.0347422469997002E-4	0.0	0.32111932207826943	0.0012158221402246477
80-81	0.0	1.0347422469997002E-4	0.0	0.3398352224708765	0.0012675592525746328
82-83	0.0	1.0347422469997002E-4	0.0	0.35822776591129624	0.001332230643012114
84-85	0.0	1.0347422469997002E-4	0.0	0.3769695348600783	0.0013451649210996103
86-87	0.0	1.0347422469997002E-4	0.0	0.39625454348853517	0.0013710334772746029
88-89	0.0	1.0347422469997002E-4	0.0	0.41790652500700387	0.0013969020334495953
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5765	0.0	45.695576	1
GGTATCA	3305	0.0	37.475742	1
TCAACGC	7370	0.0	34.93291	4
CAACGCA	7645	0.0	33.65976	5
GTACCTG	4590	0.0	33.315067	1
TATCAAC	7880	0.0	33.032425	2
ATCAACG	7795	0.0	33.028294	3
AACGCAG	8465	0.0	30.736473	6
TACCTGG	5160	0.0	29.733011	2
ACGCAGA	10245	0.0	24.979774	7
GTACATG	11830	0.0	24.724756	1
CGCAGAG	10825	0.0	23.465918	8
ACCTGGG	6410	0.0	23.194729	3
TACATGG	12330	0.0	23.152477	2
ACATGGG	12565	0.0	21.775242	3
GAGTACA	9340	0.0	19.636417	1
GCAGAGT	12830	0.0	19.576248	9
CATGGGG	9635	0.0	19.572294	4
GTATAGC	890	0.0	18.733822	1
AGTACAT	8930	0.0	18.66989	2
>>END_MODULE