FastQCFastQC Report
Mon 15 Aug 2016
SRR2921473_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921473_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227565
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35990.1615665536134748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG28450.036.8969271
GTATCAA44600.035.407631
TACCTGG29900.034.034742
TCAACGC51800.029.0258354
CAACGCA53350.028.0966135
GTACATG60350.027.5402281
ATCAACG55450.027.528553
ACCTGGG36550.027.4661243
TATCAAC56050.027.3156452
AACGCAG56250.026.9661716
GGTATCA27900.026.5680261
TACATGG64250.025.4703982
ACATGGG66200.024.0276993
ACGCAGA65000.023.119937
CGCAGAG69100.021.8758268
GAGTACA44950.021.3044951
GTATAGA7000.021.0469681
TATAACG1807.0375118E-620.3731462
AGTACAT43150.020.184292
CATGGGG51350.019.9962354
Produced by FastQC (version 0.11.5)