##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921473_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2227565
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.190679059870305	31.0	31.0	34.0	27.0	34.0
2	30.274889846087543	31.0	31.0	34.0	26.0	34.0
3	30.40963563352809	31.0	31.0	34.0	26.0	34.0
4	33.91093548336412	37.0	35.0	37.0	30.0	37.0
5	33.88819405943261	37.0	35.0	37.0	30.0	37.0
6	33.984437715622214	37.0	35.0	37.0	30.0	37.0
7	33.86842493933959	37.0	35.0	37.0	30.0	37.0
8	33.847192337821795	37.0	35.0	37.0	30.0	37.0
9	35.27664333027319	39.0	35.0	39.0	30.0	39.0
10-11	35.14505390415094	39.0	35.0	39.0	29.0	39.0
12-13	34.96885253628963	39.0	35.0	39.0	28.0	39.0
14-15	35.95354164749401	40.0	36.0	41.0	27.0	41.0
16-17	35.793963139122766	39.0	36.0	41.0	27.0	41.0
18-19	35.60197188409766	39.0	36.0	41.0	27.0	41.0
20-21	35.47809087501375	39.0	36.0	40.0	27.0	41.0
22-23	35.5153369261952	39.0	36.0	41.0	27.0	41.0
24-25	35.3692291807422	39.0	36.0	41.0	26.0	41.0
26-27	35.078986696235575	39.0	35.0	40.0	24.5	41.0
28-29	34.83038654315362	39.0	35.0	40.0	24.0	41.0
30-31	34.5570001324316	38.5	34.0	40.0	23.5	41.0
32-33	34.258293023997055	38.0	34.0	40.0	21.5	41.0
34-35	33.81550235346668	38.0	33.5	40.0	17.5	41.0
36-37	33.413397364386675	38.0	33.0	40.0	15.0	41.0
38-39	33.159005685580446	38.0	33.0	40.0	12.5	41.0
40-41	32.870952811702466	38.0	32.0	40.0	9.0	41.0
42-43	32.560563889269226	38.0	31.5	40.0	8.5	41.0
44-45	32.21427702446393	37.0	31.0	40.0	8.0	41.0
46-47	31.898816869541406	37.0	31.0	40.0	6.5	41.0
48-49	31.657872834238283	37.0	30.0	40.0	2.0	41.0
50-51	30.923146574847422	36.0	29.5	38.5	2.0	40.0
52-53	31.463084354440838	36.5	30.5	39.0	2.0	40.0
54-55	31.882776035716127	37.0	31.0	39.5	2.0	40.5
56-57	31.722292952169745	36.5	31.0	40.0	2.0	41.0
58-59	31.376106645597325	36.0	31.0	39.0	2.0	41.0
60-61	30.911299333577247	35.5	30.0	39.0	2.0	40.0
62-63	30.406147070904776	35.0	29.0	39.0	2.0	40.0
64-65	29.84206521470754	35.0	28.5	38.0	2.0	40.0
66-67	29.33689275060436	34.5	28.0	37.5	2.0	40.0
68-69	28.801881875500825	34.0	27.5	37.0	2.0	39.0
70-71	28.273500436575365	34.0	26.0	36.0	2.0	39.0
72-73	27.701998370417922	33.5	26.0	36.0	2.0	39.0
74-75	27.08774715889323	33.0	25.0	35.0	2.0	37.5
76-77	26.489671232938207	33.0	23.5	35.0	2.0	37.0
78-79	25.90996469238833	32.0	21.5	35.0	2.0	36.5
80-81	25.310512151160573	32.0	19.0	35.0	2.0	36.0
82-83	24.660937391277024	31.0	16.5	34.0	2.0	35.5
84-85	24.017928545294975	31.0	8.5	34.0	2.0	35.0
86-87	23.370115574629697	30.5	3.5	34.0	2.0	35.0
88-89	22.696494827311437	30.0	2.0	34.0	2.0	35.0
90-91	21.927660472309448	29.0	2.0	34.0	2.0	35.0
92-93	21.078196371374123	29.0	2.0	34.0	2.0	35.0
94-95	20.147118714829872	28.0	2.0	33.5	2.0	34.5
96-97	19.245934237609227	26.5	2.0	33.0	2.0	34.0
98	18.629265139288865	25.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	79286.0
3	13362.0
4	10091.0
5	8610.0
6	8132.0
7	8604.0
8	9784.0
9	11372.0
10	12638.0
11	13842.0
12	15037.0
13	17171.0
14	14859.0
15	14769.0
16	16773.0
17	19146.0
18	21323.0
19	20888.0
20	21574.0
21	23088.0
22	25280.0
23	27796.0
24	31227.0
25	34445.0
26	39016.0
27	44492.0
28	50602.0
29	58919.0
30	69305.0
31	83306.0
32	102792.0
33	131684.0
34	174228.0
35	237220.0
36	309831.0
37	310599.0
38	130571.0
39	5903.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.27857744424802	16.40389619938768	12.08533638393975	25.23218997242455
2	16.547801431053895	18.651264714429246	38.386666612769716	26.41426724174714
3	21.079747634862898	22.097401156538794	27.084469374719827	29.738381833878485
4	14.06771659838652	14.172191736582512	34.2208629574549	37.539228707576065
5	15.570656958963871	35.245330923178095	32.56571027107059	16.618301846787435
6	35.32205783529124	32.65529713626547	16.606758470997903	15.415886557445384
7	31.83035261169061	29.72363765706387	18.919341322044588	19.52666840920094
8	28.387437441797186	33.271265298864996	18.660461958595967	19.680835300741855
9	27.057491917296694	16.661211599582813	18.598635918945337	37.68266056417516
10-11	27.06984170243794	24.565631875834978	25.711180639444652	22.65334578228243
12-13	27.23263412012591	21.58571785824444	26.433779176972433	24.747868844657216
14-15	24.038459379867305	23.150384355835246	23.95407216038915	28.857084103908303
16-17	24.60322751910706	26.429266551502355	24.468756910139287	24.4987490192513
18-19	24.592335709213472	25.472769305467157	26.195212636705932	23.739682348613435
20-21	24.923055772518694	24.807838797771858	26.134395243419167	24.13471018629028
22-23	24.65298100110159	24.58822913532893	26.01649981789942	24.742290045670064
24-25	24.432462275146214	24.80958166251925	26.19876909489775	24.559186967436787
26-27	24.547537181631647	24.935628815826302	26.156558447597522	24.36027555494453
28-29	24.516495570950937	25.167817725945973	26.00263242610707	24.313054276996017
30-31	24.636847268336854	25.08147916576833	25.93833545340865	24.343338112486165
32-33	24.395433307878996	25.047696008309444	25.934907999524892	24.621962684286665
34-35	24.56115995124801	25.0849418726246	26.062550933571803	24.291347242555585
36-37	24.62468323576837	25.103607712488994	25.904435580241593	24.367273471501047
38-39	24.628809582889012	25.040454398799206	25.921453779932136	24.40928223837965
40-41	24.681827048290586	25.010136922963	25.99621795693817	24.311818071808247
42-43	24.646306104512195	24.943810283474242	25.876972621309523	24.53291099070404
44-45	24.561894441237413	25.11871400272339	25.849480438649426	24.469911117389767
46-47	24.754663591828812	25.103719365385963	25.768141580939012	24.373475461846212
48-49	24.743413611459765	25.210118920167783	25.56811810475904	24.47834936361341
50-51	24.561376588473294	25.39740744459877	25.532552490277073	24.508663476650867
52-53	24.638213032777294	25.391123792368454	25.540201839163736	24.430461335690516
54-55	24.57073240777283	25.153279213360875	25.65278566738885	24.62320271147745
56-57	24.6256626745025	25.341309386817755	25.582220384703835	24.450807553975913
58-59	24.62377340959811	25.233754720953012	25.661384674498766	24.481087194950117
60-61	24.69457012808863	25.129385015280235	25.658681709139103	24.517363147492034
62-63	24.511415335500033	25.294216017872213	25.698457666452345	24.495910980175413
64-65	24.59382951775995	25.12258417808051	25.727113839241877	24.556472464917665
66-67	24.54960487694739	25.293429668096636	25.615037254459246	24.541928200496727
68-69	24.54676349914918	25.317285861946008	25.58785561789385	24.548095021010962
70-71	24.505099622927677	25.042433888284005	25.773841867817392	24.678624620970922
72-73	24.667486809864723	25.16388017189179	25.497605535177264	24.671027483066226
74-75	24.473315620107215	25.374737548431785	25.571486035051265	24.580460796409735
76-77	24.643341744040438	25.13170247249532	25.56589611239513	24.659059671069112
78-79	24.575805483077882	25.04925362465412	25.673715397595192	24.70122549467281
80-81	24.468247264514655	25.308837118268844	25.53139195763025	24.691523659586252
82-83	24.690440304026268	25.23277729902329	25.395859546739157	24.68092285021129
84-85	24.59430266321062	25.22760060791192	25.489825185869226	24.688271543008238
86-87	24.530465464957672	25.173436143709065	25.620120170899703	24.67597822043356
88-89	24.510424116048085	24.989845724931794	25.57995513400373	24.919775025016392
90-91	24.584541266499464	25.021573120759644	25.52985771774308	24.864027894997808
92-93	24.54383853397012	25.267600146285524	25.576833495597228	24.611727824147124
94-95	24.69996152977113	25.017532592582235	25.51793871733131	24.76456716031532
96-97	24.64647343809965	25.045604328950056	25.503271110012747	24.804651122937546
98	24.653944598667728	25.08946754186125	25.47894897283686	24.77763888663416
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	430.0
1	337.5
2	471.5
3	689.0
4	501.0
5	385.5
6	717.5
7	1341.5
8	1912.5
9	1908.5
10	1447.0
11	1171.5
12	1165.5
13	1297.5
14	1484.5
15	1678.0
16	1831.0
17	1928.5
18	1988.0
19	1994.5
20	1931.0
21	1825.0
22	1857.5
23	2003.0
24	2203.5
25	2533.5
26	3125.0
27	3969.5
28	5113.0
29	6577.5
30	8440.5
31	11093.0
32	14396.0
33	17951.5
34	22034.5
35	26673.0
36	31702.0
37	36817.0
38	41928.5
39	47250.0
40	52340.0
41	58100.5
42	64814.5
43	71672.0
44	78032.5
45	83996.5
46	91250.0
47	98295.0
48	101466.0
49	101441.5
50	101125.0
51	100495.0
52	100297.0
53	100883.5
54	99861.0
55	96065.5
56	89917.0
57	83727.0
58	77574.0
59	70463.0
60	62071.0
61	52243.5
62	42281.0
63	32607.0
64	24489.0
65	18668.5
66	14249.0
67	10822.0
68	8347.5
69	6557.5
70	4974.5
71	3837.5
72	3135.5
73	2436.5
74	1880.5
75	1497.0
76	1099.5
77	696.0
78	410.5
79	245.0
80	150.0
81	85.5
82	53.0
83	41.0
84	28.5
85	16.0
86	12.0
87	9.5
88	7.0
89	7.5
90	6.0
91	5.5
92	4.5
93	2.5
94	2.5
95	1.5
96	0.5
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.48205102881397405
2	0.04911192265994482
3	0.05687825046631636
4	0.05378069775741673
5	0.06415076552199375
6	0.061995946246237485
7	0.04143537899006314
8	0.01970761795952082
9	0.012479994972088357
10-11	0.015061288896171381
12-13	0.01131280119772038
14-15	0.014904166657314153
16-17	0.014028771326538172
18-19	0.05216458330059953
20-21	0.07921205441816512
22-23	0.09636082448772539
24-25	0.06641781496836231
26-27	0.08089550697734971
28-29	0.08356658503792258
30-31	0.055172351873009326
32-33	0.031491785873812884
34-35	0.40353480145360515
36-37	0.636030822894057
38-39	0.5851456635384377
40-41	0.5786138676088015
42-43	0.5522622235490322
44-45	0.53628064725384
46-47	0.5265839605129368
48-49	0.5161914467142372
50-51	0.5296141751194691
52-53	0.6116095377688193
54-55	0.5826541537508445
56-57	0.539445538065107
58-59	0.5168648277379112
60-61	0.5294794989147342
62-63	0.5413310049313936
64-65	0.5591082639563829
66-67	0.5865148716199078
68-69	0.5411738826925364
70-71	0.48849304060712034
72-73	0.4701321846949472
74-75	0.44950427933640547
76-77	0.4647002444373116
78-79	0.4585949231560022
80-81	0.454936219594041
82-83	0.4752049884066234
84-85	0.4403687434485638
86-87	0.44398255494228
88-89	0.4721972198342136
90-91	0.4537016877173057
92-93	0.4482024093573027
94-95	0.4608619726023708
96-97	0.45439751477510193
98	0.4489655745174664
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	2227565.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.21079829161905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.02441103632485	49.16171695094799
2	12.303253489296724	15.307904423065935
3	3.854375314150886	7.193512956265097
4	1.6292873432882846	4.054370650895814
5	0.8582166302178788	2.669517083649873
6	0.5102304807409866	1.9045107311768001
7	0.34504601037203336	1.5025911426807723
8	0.24047884426233956	1.196830469904486
9	0.18653464099205355	1.0444022032640596
>10	0.9708276337864384	10.781589566464785
>50	0.05516068226471346	2.33952005712501
>100	0.0210922445174155	2.142117284572331
>500	7.961431759186164E-4	0.31077009531857536
>1k	2.8950660942473656E-4	0.3906463846685481
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3599	0.1615665536134748	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.489206824492215E-5
2	0.0	0.0	0.0	4.489206824492215E-5	4.489206824492215E-5
3	0.0	0.0	0.0	4.489206824492215E-5	4.489206824492215E-5
4	0.0	0.0	0.0	4.489206824492215E-5	8.97841364898443E-5
5	0.0	0.0	0.0	4.489206824492215E-5	8.97841364898443E-5
6	0.0	0.0	0.0	4.489206824492215E-5	8.97841364898443E-5
7	0.0	0.0	0.0	4.489206824492215E-5	8.97841364898443E-5
8	0.0	0.0	0.0	4.489206824492215E-5	8.97841364898443E-5
9	0.0	0.0	0.0	8.97841364898443E-5	8.97841364898443E-5
10-11	4.489206824492215E-5	0.0	0.0	8.97841364898443E-5	8.97841364898443E-5
12-13	4.489206824492215E-5	0.0	0.0	1.3467620473476644E-4	1.571222388572275E-4
14-15	4.489206824492215E-5	2.2446034122461074E-5	0.0	1.795682729796886E-4	1.795682729796886E-4
16-17	4.489206824492215E-5	4.489206824492215E-5	0.0	1.795682729796886E-4	1.795682729796886E-4
18-19	4.489206824492215E-5	8.97841364898443E-5	0.0	1.795682729796886E-4	1.795682729796886E-4
20-21	4.489206824492215E-5	8.97841364898443E-5	0.0	2.6935240946953287E-4	2.2446034122461072E-4
22-23	4.489206824492215E-5	8.97841364898443E-5	0.0	3.8158258008183824E-4	2.6935240946953287E-4
24-25	4.489206824492215E-5	8.97841364898443E-5	0.0	4.938127506941436E-4	2.9179844359199393E-4
26-27	4.489206824492215E-5	8.97841364898443E-5	0.0	6.733810236738322E-4	3.14244477714455E-4
28-29	4.489206824492215E-5	8.97841364898443E-5	0.0	0.0012794239449802811	3.14244477714455E-4
30-31	4.489206824492215E-5	8.97841364898443E-5	0.0	0.0037933797666959215	3.14244477714455E-4
32-33	4.489206824492215E-5	8.97841364898443E-5	0.0	0.00857438503478013	3.14244477714455E-4
34-35	4.489206824492215E-5	8.97841364898443E-5	0.0	0.015487763544498141	3.591365459593772E-4
36-37	4.489206824492215E-5	8.97841364898443E-5	0.0	0.024982435978299175	3.591365459593772E-4
38-39	4.489206824492215E-5	8.97841364898443E-5	0.0	0.03665437372197893	3.591365459593772E-4
40-41	4.489206824492215E-5	8.97841364898443E-5	0.0	0.05063825298027218	3.591365459593772E-4
42-43	4.489206824492215E-5	8.97841364898443E-5	0.0	0.06442011793146328	4.040286142042993E-4
44-45	4.489206824492215E-5	8.97841364898443E-5	0.0	0.0798629894077165	4.713667165716825E-4
46-47	4.489206824492215E-5	8.97841364898443E-5	0.0	0.0968097451701746	5.835968871839879E-4
48-49	4.489206824492215E-5	8.97841364898443E-5	0.0	0.11346470248904073	6.733810236738322E-4
50-51	4.489206824492215E-5	8.97841364898443E-5	0.0	0.12958095498896777	7.182730919187544E-4
52-53	4.489206824492215E-5	8.97841364898443E-5	0.0	0.1461236821372216	7.407191260412154E-4
54-55	4.489206824492215E-5	1.3467620473476644E-4	0.0	0.1639907252987006	8.080572284085986E-4
56-57	4.489206824492215E-5	1.3467620473476644E-4	0.0	0.18125172553887314	8.080572284085986E-4
58-59	4.489206824492215E-5	1.3467620473476644E-4	0.0	0.20037574661121	8.080572284085986E-4
60-61	4.489206824492215E-5	1.3467620473476644E-4	0.0	0.21873660252338314	8.080572284085986E-4
62-63	4.489206824492215E-5	1.3467620473476644E-4	0.0	0.23626695517302526	8.529492966535207E-4
64-65	4.489206824492215E-5	1.3467620473476644E-4	0.0	0.2548073793581781	8.529492966535207E-4
66-67	4.489206824492215E-5	1.3467620473476644E-4	0.0	0.2742680909423518	8.529492966535207E-4
68-69	4.489206824492215E-5	1.3467620473476644E-4	0.0	0.2947837661302813	8.529492966535207E-4
70-71	4.489206824492215E-5	1.3467620473476644E-4	0.0	0.31653397319494603	8.529492966535207E-4
72-73	4.489206824492215E-5	1.571222388572275E-4	0.0	0.33821684215724346	0.0010100715355107483
74-75	4.489206824492215E-5	1.795682729796886E-4	0.0	0.3602812936996227	0.0011223017061230537
76-77	4.489206824492215E-5	2.0201430710214965E-4	0.0	0.38108876733114405	0.0011223017061230537
78-79	4.489206824492215E-5	2.2446034122461072E-4	0.0	0.4047019952279731	0.0011896398084904369
80-81	4.489206824492215E-5	2.2446034122461072E-4	0.0	0.4275520579646385	0.0013018699791027422
82-83	4.489206824492215E-5	2.2446034122461072E-4	0.0	0.4521304653287334	0.0014814382520824307
84-85	4.489206824492215E-5	2.6935240946953287E-4	0.0	0.47691088699993045	0.0014814382520824307
86	4.489206824492215E-5	2.6935240946953287E-4	0.0	0.4936780744894088	0.001526330320327353
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	2845	0.0	36.896927	1
GTATCAA	4460	0.0	35.40763	1
TACCTGG	2990	0.0	34.03474	2
TCAACGC	5180	0.0	29.025835	4
CAACGCA	5335	0.0	28.096613	5
GTACATG	6035	0.0	27.540228	1
ATCAACG	5545	0.0	27.52855	3
ACCTGGG	3655	0.0	27.466124	3
TATCAAC	5605	0.0	27.315645	2
AACGCAG	5625	0.0	26.966171	6
GGTATCA	2790	0.0	26.568026	1
TACATGG	6425	0.0	25.470398	2
ACATGGG	6620	0.0	24.027699	3
ACGCAGA	6500	0.0	23.11993	7
CGCAGAG	6910	0.0	21.875826	8
GAGTACA	4495	0.0	21.304495	1
GTATAGA	700	0.0	21.046968	1
TATAACG	180	7.0375118E-6	20.373146	2
AGTACAT	4315	0.0	20.18429	2
CATGGGG	5135	0.0	19.996235	4
>>END_MODULE