FastQCFastQC Report
Mon 15 Aug 2016
SRR2921473_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921473_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227565
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34960.15694267058424782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG30250.039.6815641
GTATCAA41200.039.4249151
TACCTGG31250.036.478162
GGTATCA24400.034.3586771
TCAACGC50100.031.0969374
CAACGCA51800.030.0621855
TATCAAC54600.029.1423032
ATCAACG54200.028.7445853
AACGCAG55300.028.5029226
ACCTGGG39850.028.0105083
GTACATG60150.026.7666911
TACATGG63350.024.8921952
ACGCAGA68050.023.2333647
ACATGGG63800.022.6325973
CGCAGAG72250.021.4227738
GAGTACA47300.021.3495311
AGTACAT43750.020.9532282
CATGGGG48750.019.4866354
GCAGAGT80550.019.2140439
CCTGGGG41400.019.0453624
Produced by FastQC (version 0.11.5)