##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921473_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2227565
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.951217585120972	33.0	31.0	34.0	30.0	34.0
2	32.05574158329836	34.0	31.0	34.0	30.0	34.0
3	32.18515464195209	34.0	31.0	34.0	30.0	34.0
4	35.722546367894985	37.0	35.0	37.0	35.0	37.0
5	35.52924426447713	37.0	35.0	37.0	33.0	37.0
6	35.56652308686839	37.0	35.0	37.0	33.0	37.0
7	35.498380518638065	37.0	35.0	37.0	33.0	37.0
8	35.44973637132923	37.0	35.0	37.0	33.0	37.0
9	37.0736095243012	39.0	37.0	39.0	34.0	39.0
10-11	36.98412594020826	39.0	37.0	39.0	33.5	39.0
12-13	36.86440193664382	39.0	37.0	39.0	33.0	39.0
14-15	38.16783662878524	40.0	38.0	41.0	33.0	41.0
16-17	38.07458345772177	40.0	38.0	41.0	33.0	41.0
18-19	37.96496555656064	40.0	38.0	41.0	33.0	41.0
20-21	37.85009258989075	40.0	38.0	41.0	33.0	41.0
22-23	37.81208898505768	40.0	38.0	41.0	33.0	41.0
24-25	37.86789184602918	40.0	38.0	41.0	33.0	41.0
26-27	37.75822187006889	40.0	38.0	41.0	33.0	41.0
28-29	37.62296655765376	40.0	38.0	41.0	32.0	41.0
30-31	37.47986815199557	40.0	37.5	41.0	32.0	41.0
32-33	37.312354072720666	40.0	37.0	41.0	32.0	41.0
34-35	37.163819237598005	40.0	37.0	41.0	31.0	41.0
36-37	37.06195307432107	40.0	37.0	41.0	31.0	41.0
38-39	37.030353996404145	40.0	37.0	41.0	31.0	41.0
40-41	36.860019348481416	40.0	36.5	41.0	31.0	41.0
42-43	36.61634924233412	39.5	36.0	41.0	30.0	41.0
44-45	36.401700511545116	39.0	36.0	41.0	30.0	41.0
46-47	36.1198833704067	39.0	35.0	40.5	29.5	41.0
48-49	35.777696722654554	39.0	35.0	40.0	28.5	41.0
50-51	35.49484257473968	38.5	35.0	40.0	28.0	41.0
52-53	35.17890005454386	38.0	34.5	40.0	27.0	41.0
54-55	34.84928228805894	38.0	34.0	40.0	26.5	41.0
56-57	34.468859494560206	38.0	34.0	40.0	26.0	41.0
58-59	34.08033929425179	37.0	33.0	40.0	25.0	41.0
60-61	33.65057697530712	36.5	33.0	39.0	24.0	41.0
62-63	33.203322461970814	36.0	32.5	39.0	22.5	40.0
64-65	32.690052815518285	35.5	32.0	39.0	22.0	40.0
66-67	32.164992491801584	35.0	31.0	38.0	20.0	40.0
68-69	31.66145769034798	35.0	31.0	37.5	18.5	39.5
70-71	31.114737617084124	34.5	30.0	37.0	17.5	39.0
72-73	30.57056000610532	34.0	30.0	36.0	13.0	39.0
74-75	30.050326926486996	34.0	29.0	35.5	9.0	38.0
76-77	28.780229308684596	32.5	28.0	34.5	8.5	36.0
78-79	29.7117673333887	34.0	29.5	35.0	7.5	37.0
80-81	29.745158278209615	34.0	30.0	35.0	7.0	36.5
82-83	29.40965605941914	34.0	29.5	35.0	3.5	36.0
84-85	28.920019617833823	34.0	29.0	35.0	2.0	36.0
86-87	28.397674366404573	33.5	28.5	35.0	2.0	35.0
88-89	27.852286689726228	33.0	27.0	35.0	2.0	35.0
90-91	27.380757239407156	33.0	26.0	35.0	2.0	35.0
92-93	26.945684862170125	33.0	25.5	34.0	2.0	35.0
94-95	26.443848327658227	32.0	25.0	34.0	2.0	35.0
96-97	25.677676745684188	32.0	23.5	34.0	2.0	35.0
98-99	24.66976429419568	32.0	11.0	34.0	2.0	35.0
100-101	21.11738535126921	27.5	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	14493.0
3	7647.0
4	4512.0
5	3497.0
6	3061.0
7	3052.0
8	3597.0
9	4338.0
10	5080.0
11	5952.0
12	7128.0
13	8689.0
14	9550.0
15	10847.0
16	12466.0
17	14750.0
18	14823.0
19	13018.0
20	12846.0
21	13566.0
22	14426.0
23	15964.0
24	18231.0
25	21295.0
26	25180.0
27	30521.0
28	37335.0
29	45296.0
30	55457.0
31	68952.0
32	89018.0
33	118778.0
34	169012.0
35	255491.0
36	394148.0
37	480416.0
38	208331.0
39	6802.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.578845254855125	16.85946646562525	12.455695678059914	25.105992601459715
2	16.72130779573211	18.882456853110906	38.063894880732995	26.332340470423983
3	21.018246695612003	22.190725849717406	27.287660999265068	29.503366455405523
4	14.251050189914583	14.429735197622565	34.08364865981752	37.23556595264533
5	15.595471963741234	35.21335605491968	32.6972665859799	16.493905395359192
6	35.495339118387875	32.756804446918146	16.40874703121764	15.339109403476334
7	31.971503971060095	29.640174063527976	18.932104262004607	19.456217703407322
8	28.484758604883176	33.42883079647132	18.456446415811683	19.629964182833824
9	27.01305248854627	16.674185657360493	18.527092630969737	37.785669223123506
10-11	27.03970874174691	24.70430879414248	25.715866825381205	22.540115638729404
12-13	27.145569021582467	21.587296095141784	26.524847616905678	24.742287266370074
14-15	23.987254785912793	23.293055695624236	23.994037978240325	28.725651540222646
16-17	24.58086521491155	26.441441036856737	24.576733595900716	24.400960152330995
18-19	24.54545025860232	25.58594482416996	26.226022895506162	23.642582021721562
20-21	24.888135278122114	24.819879218207664	26.23428398607307	24.057701517597152
22-23	24.608275103526932	24.598259808869553	26.202121670560132	24.591343417043383
24-25	24.427301454287516	24.853705640997315	26.29335305705306	24.425639847662108
26-27	24.478915029698026	24.99501590420478	26.243105334149945	24.282963731947245
28-29	24.400340991267992	25.153943368238462	26.206687676650116	24.23902796384343
30-31	24.562407702082357	25.080454491276438	26.06485337944778	24.29228442719343
32-33	24.299091853709815	25.020791717163508	26.06930033655084	24.610816092575835
34-35	24.429916242852627	25.08743409332529	26.27808665919605	24.20456300462604
36-37	24.497563016758974	25.16075199879318	26.051252074219338	24.290432910228503
38-39	24.453497537189108	25.12204052430113	26.087409413884455	24.337052524625303
40-41	24.56626499261945	25.05188835444601	26.05642893624664	24.325417716687898
42-43	24.554715918082408	25.06262114292705	25.99690457725257	24.38575836173797
44-45	24.46862389077763	25.062229597581936	26.027776131492125	24.441370380148307
46-47	24.566137277472546	25.132841236452347	25.964363276972772	24.336658209102332
48-49	24.559472700573423	25.313411756044108	25.711189355246077	24.415926188136392
50-51	24.47742639613166	25.411738772347388	25.671793764381874	24.439041067139083
52-53	24.60801557971405	25.39025603453539	25.61979464083695	24.38193374491361
54-55	24.576755699805137	25.165518763132916	25.75113924272262	24.506586294339332
56-57	24.535356539598986	25.359199546574335	25.663264103614498	24.442179810212178
58-59	24.650635251485607	25.296705832390217	25.64742857938096	24.40523033674322
60-61	24.71513095603133	25.168299567168756	25.695320205780032	24.42124927101988
62-63	24.447299986350867	25.35999583342313	25.789472030056825	24.403232150169178
64-65	24.561033317021266	25.157067240133067	25.742820071922743	24.53907937092292
66-67	24.555188191166007	25.377850891375108	25.623082339922536	24.443878577536342
68-69	24.584879975617575	25.349795514181494	25.632250745498986	24.433073764701945
70-71	24.487876298759534	25.207367137205445	25.764753659132854	24.54000290490217
72-73	24.7384389053099	25.19918520654098	25.55864366348096	24.50373222466816
74-75	24.51180353654697	25.451523057982605	25.47463201954358	24.562041385926843
76-77	24.554423880056657	25.25576399128492	25.639035667478783	24.550776461179645
78-79	24.546017549428104	25.19090725507096	25.749359411955115	24.513715783545823
80-81	24.485181864498156	25.45496718021054	25.560298574422326	24.49955238086898
82-83	24.597706156089338	25.408894898193328	25.440440752843507	24.552958192873824
84-85	24.543467875045575	25.42532584741881	25.519348790094455	24.51185748744116
86-87	24.51957607274002	25.40437590734007	25.598271390752885	24.47777662916702
88-89	24.47222088081356	25.250062394307655	25.575708779025764	24.702007945853026
90-91	24.53119784953245	25.293997997422046	25.56750384614088	24.607300306904623
92-93	24.571489526547158	25.527445443163764	25.600812948792083	24.300252081496993
94-95	24.555294588784285	25.34112058378339	25.48664890456435	24.61693592286797
96-97	24.48151156889202	25.36729607550841	25.58191267589257	24.569279679707005
98-99	24.58844353255581	25.3470071732662	25.506028978154703	24.558520316023287
100-101	25.090042616617676	24.82206411685281	25.64577588171055	24.442117384818967
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	238.0
1	213.5
2	424.0
3	621.0
4	429.0
5	318.5
6	601.0
7	1212.0
8	1759.5
9	1717.5
10	1201.5
11	797.0
12	668.0
13	664.5
14	731.0
15	783.0
16	822.5
17	893.0
18	972.5
19	1075.5
20	1149.5
21	1204.5
22	1351.0
23	1550.0
24	1751.5
25	2058.0
26	2546.5
27	3304.0
28	4290.0
29	5573.5
30	7349.5
31	9617.5
32	12449.5
33	15681.0
34	19056.5
35	23292.5
36	28387.0
37	33216.0
38	37911.5
39	42812.0
40	47906.5
41	52965.5
42	58656.0
43	65621.5
44	73114.0
45	80842.0
46	86104.0
47	91013.5
48	98305.5
49	102201.5
50	101122.5
51	99505.0
52	99420.0
53	99169.0
54	98469.0
55	95503.5
56	89184.5
57	82106.5
58	75843.0
59	69606.5
60	61194.0
61	51726.5
62	42443.0
63	32581.0
64	24141.0
65	18409.5
66	13994.0
67	10707.0
68	8320.5
69	6440.0
70	5049.0
71	3954.0
72	3019.0
73	2414.5
74	1906.5
75	1441.5
76	1044.0
77	653.0
78	403.0
79	226.0
80	131.5
81	68.0
82	31.0
83	17.5
84	8.0
85	3.5
86	2.0
87	1.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.31994577038156014
2	0.0
3	0.006644026100248477
4	0.05840458078664371
5	9.42733433143365E-4
6	0.06661982927546446
7	0.07681032876706179
8	0.10661866208169009
9	0.09377953056364237
10-11	0.09813406118339982
12-13	0.09743823412560351
14-15	0.06619335462713771
16-17	0.037484876984509995
18-19	0.03966214229438872
20-21	0.029471642802791388
22-23	0.04356775223169695
24-25	0.03611566890303987
26-27	0.021593084825807553
28-29	0.023725458067441352
30-31	0.0028506463335525563
32-33	0.005185033882288508
34-35	0.007766327806371531
36-37	0.009921147082127794
38-39	0.014971504759681535
40-41	0.019191359174704218
42-43	0.0037035956302060766
44-45	0.014769490452579387
46-47	0.02534157252425855
48-49	0.0028730923676750174
50-51	0.006801148339105705
52-53	0.0023792796169808737
54-55	0.004421868722124831
56-57	0.0034791352889814667
58-59	9.876255013882872E-4
60-61	0.006195105417799256
62-63	0.01423078563364032
64-65	0.007856111942861375
66-67	0.040088616942715476
68-69	0.06156947159791072
70-71	0.013265606166374495
72-73	0.03335480670597715
74-75	0.051895230891130004
76-77	0.3060067831915118
78-79	0.07544112068559167
80-81	0.035172935469896506
82-83	0.029090060222709553
84-85	0.02015653864197004
86-87	0.11918844119026831
88-89	0.5305344625184899
90-91	0.1316459901282342
92-93	0.04949350524002667
94-95	0.007339853158044771
96-97	0.004691221131594364
98-99	0.008866183478372125
100-101	0.006621580066126016
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2227565.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.91316401295033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.55822411350589	43.75815825612725
2	14.153691263081294	16.393700870149786
3	4.536221626556883	7.881208411736434
4	1.8890458124508673	4.376024798577764
5	1.0438445568939956	3.0226170513713715
6	0.6171171998238695	2.1443525765167446
7	0.4210030543607308	1.7067133256002236
8	0.286081390729289	1.3254302801886597
9	0.22975387360912922	1.1975196388442546
>10	1.167513871002822	12.12112355690679
>50	0.06981361230084948	2.766917966461272
>100	0.026446701296421314	2.5479962042672155
>500	8.545105168302286E-4	0.30695689347165145
>1k	3.884138712863175E-4	0.4512801697806178
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3496	0.15694267058424782	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3467620473476644E-4
2	0.0	0.0	0.0	0.0	1.3467620473476644E-4
3	0.0	0.0	0.0	0.0	1.3467620473476644E-4
4	0.0	0.0	0.0	4.489206824492215E-5	1.3467620473476644E-4
5	0.0	0.0	0.0	4.489206824492215E-5	1.795682729796886E-4
6	0.0	0.0	0.0	4.489206824492215E-5	2.2446034122461072E-4
7	0.0	0.0	0.0	4.489206824492215E-5	2.2446034122461072E-4
8	0.0	0.0	0.0	4.489206824492215E-5	2.2446034122461072E-4
9	0.0	0.0	0.0	4.489206824492215E-5	2.2446034122461072E-4
10-11	4.489206824492215E-5	0.0	0.0	4.489206824492215E-5	2.2446034122461072E-4
12-13	4.489206824492215E-5	0.0	0.0	8.97841364898443E-5	3.14244477714455E-4
14-15	4.489206824492215E-5	0.0	0.0	8.97841364898443E-5	3.591365459593772E-4
16-17	4.489206824492215E-5	0.0	0.0	1.3467620473476644E-4	4.040286142042993E-4
18-19	4.489206824492215E-5	0.0	0.0	1.795682729796886E-4	5.611508530615269E-4
20-21	4.489206824492215E-5	0.0	0.0	2.2446034122461072E-4	6.2848895542891E-4
22-23	4.489206824492215E-5	0.0	0.0	2.469063753470718E-4	6.509349895513711E-4
24-25	4.489206824492215E-5	0.0	0.0	3.591365459593771E-4	6.733810236738322E-4
26-27	4.489206824492215E-5	0.0	0.0	5.387048189390657E-4	7.856111942861376E-4
28-29	4.489206824492215E-5	0.0	0.0	0.0011447477402455146	8.080572284085986E-4
30-31	4.489206824492215E-5	0.0	0.0	0.003568919425471311	8.080572284085986E-4
32-33	4.489206824492215E-5	0.0	0.0	0.00834992469355552	8.080572284085986E-4
34-35	4.489206824492215E-5	0.0	0.0	0.015285749237395991	8.529492966535207E-4
36-37	4.489206824492215E-5	0.0	0.0	0.024825313739441947	8.529492966535207E-4
38-39	4.489206824492215E-5	2.2446034122461074E-5	0.0	0.03656458958548908	8.978413648984429E-4
40-41	8.97841364898443E-5	4.489206824492215E-5	0.0	0.050750483150884484	8.978413648984429E-4
42-43	8.97841364898443E-5	4.489206824492215E-5	0.0	0.06462213223856543	9.20287399020904E-4
44-45	8.97841364898443E-5	4.489206824492215E-5	0.0	0.08022212595367587	9.427334331433651E-4
46-47	8.97841364898443E-5	4.489206824492215E-5	0.0	0.09723621981850136	9.427334331433651E-4
48-49	8.97841364898443E-5	4.489206824492215E-5	0.0	0.11373405489851027	9.427334331433651E-4
50-51	8.97841364898443E-5	4.489206824492215E-5	0.0	0.12994009153492714	9.876255013882872E-4
52-53	8.97841364898443E-5	4.489206824492215E-5	0.0	0.14637058851256865	9.876255013882872E-4
54-55	8.97841364898443E-5	4.489206824492215E-5	0.0	0.16399072529870062	9.876255013882872E-4
56-57	8.97841364898443E-5	4.489206824492215E-5	0.0	0.18089258899291377	0.0010100715355107481
58-59	1.3467620473476644E-4	4.489206824492215E-5	0.0	0.1996350274851688	0.0010325175696332092
60-61	1.3467620473476644E-4	4.489206824492215E-5	0.0	0.21759185478313764	0.0010325175696332092
62-63	1.3467620473476644E-4	4.489206824492215E-5	0.0	0.23485285502331021	0.0010325175696332092
64-65	1.3467620473476644E-4	4.489206824492215E-5	0.0	0.25325860300372827	0.0010774096378781315
66-67	1.3467620473476644E-4	4.489206824492215E-5	0.0	0.27242751614431004	0.0010998556720005926
68-69	1.3467620473476644E-4	4.489206824492215E-5	0.0	0.2924493785815453	0.0011223017061230537
70-71	1.3467620473476644E-4	4.489206824492215E-5	0.0	0.3138628951343732	0.0011223017061230537
72-73	1.3467620473476644E-4	4.489206824492215E-5	0.0	0.33559065616491546	0.001167193774367976
74-75	1.3467620473476644E-4	4.489206824492215E-5	0.0	0.35747553943431504	0.0012345318767353589
76-77	1.3467620473476644E-4	4.489206824492215E-5	0.0	0.37805855272461186	0.0013018699791027422
78-79	1.3467620473476644E-4	4.489206824492215E-5	0.0	0.4011555218366243	0.0013018699791027422
80-81	1.3467620473476644E-4	4.489206824492215E-5	0.0	0.4233546495837383	0.0013243160132252033
82-83	1.3467620473476644E-4	4.489206824492215E-5	0.0	0.4471698917876695	0.0013467620473476645
84-85	1.3467620473476644E-4	4.489206824492215E-5	0.0	0.4715238388105398	0.0014141001497150476
86-87	1.3467620473476644E-4	4.489206824492215E-5	0.0	0.49563087945806295	0.001548776354449814
88-89	1.3467620473476644E-4	4.489206824492215E-5	2.2446034122461074E-5	0.5218254012789749	0.0016161144568171972
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	3025	0.0	39.681564	1
GTATCAA	4120	0.0	39.424915	1
TACCTGG	3125	0.0	36.47816	2
GGTATCA	2440	0.0	34.358677	1
TCAACGC	5010	0.0	31.096937	4
CAACGCA	5180	0.0	30.062185	5
TATCAAC	5460	0.0	29.142303	2
ATCAACG	5420	0.0	28.744585	3
AACGCAG	5530	0.0	28.502922	6
ACCTGGG	3985	0.0	28.010508	3
GTACATG	6015	0.0	26.766691	1
TACATGG	6335	0.0	24.892195	2
ACGCAGA	6805	0.0	23.233364	7
ACATGGG	6380	0.0	22.632597	3
CGCAGAG	7225	0.0	21.422773	8
GAGTACA	4730	0.0	21.349531	1
AGTACAT	4375	0.0	20.953228	2
CATGGGG	4875	0.0	19.486635	4
GCAGAGT	8055	0.0	19.214043	9
CCTGGGG	4140	0.0	19.045362	4
>>END_MODULE